Signal peptide replacement resulted in recombinant homologous expression of laccase Lcc8 in Coprinopsis cinerea View Full Text


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Article Info

DATE

2019-12

AUTHORS

Marcus Schulze, Lukas Geisler, Andrzej Majcherczyk, Martin Rühl

ABSTRACT

Although the model agaricomycete Coprinopsis cinerea possess 17 different laccase genes, up to now only four C. cinerea laccases have been purified and characterized to some degree. By exchanging the nucleotide sequence of the deduced signal peptide of Lcc8 it was possible to homologously express lcc8 in C. cinerea under control of the Agaricus bisporus gdpII promoter and the C. cinerea lcc1 terminator. The purified Lcc8 showed two bands in the SDS-PAGE with a molecular weight of 64 kDa and 77 kDa, respectively. The IEF determined pI values of 3.3 and 3.4 for both bands. The optimal pH for oxidation of the substrates ABTS, 2,6-dimethoxyphenol, guaiacol and syringaldazine was pH 4.0, pH 5.0, pH 4.5 and pH 5.0, respectively. Best pH for enzyme storage was pH 8.0. The optimal temperature for oxidation of ABTS was 63 °C, while Lcc8 showed activity of at least 50% over 300 min at 50 °C. The comparable high stability of Lcc8 at alkaline pH and higher temperatures can be of interest for biotechnical applications. More... »

PAGES

36

References to SciGraph publications

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  • Journal

    TITLE

    AMB Express

    ISSUE

    1

    VOLUME

    9

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13568-019-0761-1

    DOI

    http://dx.doi.org/10.1186/s13568-019-0761-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112770924

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30874916


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    JSON-LD is a popular format for linked data which is fully compatible with JSON.

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    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13568-019-0761-1'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13568-019-0761-1'

    RDF/XML is a standard XML format for linked data.

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