Characterization of carp seminal plasma Wap65-2 and its participation in the testicular immune response and temperature acclimation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2020-11-25

AUTHORS

Mariola A. Dietrich, Mikołaj Adamek, Verena Jung-Schroers, Krzysztof Rakus, Magdalena Chadzińska, Anna Hejmej, Piotr Hliwa, Barbara Bilińska, Halina Karol, Andrzej Ciereszko

ABSTRACT

Two functionally distinct isoforms of warm-temperature acclimation related 65-kDa protein (Wap65-1 and Wap65-2) with a role in the immune response are present in fish. To our knowledge, contrary to Wap65-1, Wap65-2 has neither been isolated nor functionally characterized in carp especially in reproductive system. The aim of this study was to characterize Wap65-2 and ascertain its functions in immune response and temperature acclimation within reproductive system. Wap65-2 corresponded to one of the most abundant proteins in carp seminal plasma, with a high immunologic similarity to their counterparts in seminal plasma of other fish species and a wide tissue distribution, with predominant expression in the liver. The immunohistochemical localization of Wap65-2 to spermatogonia, Leydig cells, and the epithelium of blood vessels within the testis suggests its role in iron metabolism during spermatogenesis and maintenance of blood-testis barrier integrity. Wap65-2 secretion by the epithelial cells of the spermatic duct and its presence around spermatozoa suggests its involvement in the protection of spermatozoa against damage caused by heme released from erythrocytes following hemorrhage and inflammation. Our results revealed an isoform-specific response of Wap65 to temperature acclimation and Aeromonas salmonicida infection which alters blood-testis barrier integrity. Wap65-2 seems to be related to the immune response against bacteria, while Wap65-1 seems to be involved in temperature acclimation. This study expands the understanding of the mechanism of carp testicular immunity against bacterial challenge and temperature changes, in which Wap65-2 seems to be involved and highlights their potential usefulness as biomarkers of inflammation and temperature acclimation. More... »

PAGES

142

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13567-020-00858-x

DOI

http://dx.doi.org/10.1186/s13567-020-00858-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1132925010

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/33239112


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29 schema:description Two functionally distinct isoforms of warm-temperature acclimation related 65-kDa protein (Wap65-1 and Wap65-2) with a role in the immune response are present in fish. To our knowledge, contrary to Wap65-1, Wap65-2 has neither been isolated nor functionally characterized in carp especially in reproductive system. The aim of this study was to characterize Wap65-2 and ascertain its functions in immune response and temperature acclimation within reproductive system. Wap65-2 corresponded to one of the most abundant proteins in carp seminal plasma, with a high immunologic similarity to their counterparts in seminal plasma of other fish species and a wide tissue distribution, with predominant expression in the liver. The immunohistochemical localization of Wap65-2 to spermatogonia, Leydig cells, and the epithelium of blood vessels within the testis suggests its role in iron metabolism during spermatogenesis and maintenance of blood-testis barrier integrity. Wap65-2 secretion by the epithelial cells of the spermatic duct and its presence around spermatozoa suggests its involvement in the protection of spermatozoa against damage caused by heme released from erythrocytes following hemorrhage and inflammation. Our results revealed an isoform-specific response of Wap65 to temperature acclimation and Aeromonas salmonicida infection which alters blood-testis barrier integrity. Wap65-2 seems to be related to the immune response against bacteria, while Wap65-1 seems to be involved in temperature acclimation. This study expands the understanding of the mechanism of carp testicular immunity against bacterial challenge and temperature changes, in which Wap65-2 seems to be involved and highlights their potential usefulness as biomarkers of inflammation and temperature acclimation.
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36 schema:keywords Aeromonas salmonicida infection
37 Immunohistochemical localization
38 Leydig cells
39 Wap65
40 Wap65-1
41 Wap65-2
42 Wap65-2 secretion
43 abundant proteins
44 acclimation
45 aim
46 alters blood-testis barrier integrity
47 bacteria
48 bacterial challenge
49 barrier integrity
50 biomarkers
51 biomarkers of inflammation
52 blood vessels
53 blood-testis barrier integrity
54 carp
55 carp seminal plasma
56 carp seminal plasma Wap65-2
57 carp testicular immunity
58 cells
59 challenges
60 changes
61 characterization
62 counterparts
63 damage
64 distinct isoforms
65 distribution
66 duct
67 epithelial cells
68 epithelium
69 erythrocytes
70 expression
71 fish
72 fish species
73 function
74 heme
75 hemorrhage
76 high immunologic similarity
77 immune response
78 immunity
79 immunologic similarities
80 infection
81 inflammation
82 integrity
83 involvement
84 iron metabolism
85 isoform-specific responses
86 isoforms
87 knowledge
88 liver
89 localization
90 maintenance
91 mechanism
92 metabolism
93 participation
94 plasma
95 plasma Wap65-2
96 potential usefulness
97 predominant expression
98 presence
99 protection
100 protection of spermatozoa
101 protein
102 reproductive system
103 response
104 results
105 role
106 salmonicida infection
107 secretion
108 seminal plasma
109 seminal plasma Wap65-2
110 similarity
111 species
112 spermatic duct
113 spermatogenesis
114 spermatogonia
115 spermatozoa
116 study
117 system
118 temperature acclimation
119 temperature changes
120 testicular immune response
121 testicular immunity
122 testis
123 tissue distribution
124 understanding
125 usefulness
126 vessels
127 warm temperature acclimation
128 wide tissue distribution
129 schema:name Characterization of carp seminal plasma Wap65-2 and its participation in the testicular immune response and temperature acclimation
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