Melatonin in cryopreservation media improves transplantation efficiency of frozen–thawed spermatogonial stem cells into testes of azoospermic mice View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2022-07-26

AUTHORS

Shokoofeh Kazemzadeh, Shahram Mohammadpour, Soheila Madadi, Azar Babakhani, Maryam Shabani, Maryam Khanehzad

ABSTRACT

BackgroundCryostorage of spermatogonial stem cells (SSCs) is an appropriate procedure for long-term storage of SSCs for fertility preservation. However, it causes damage to cellular structures through overproduction of ROS and oxidative stress. In this study, we examined the protective effect of melatonin as a potent antioxidant in the basic freezing medium to establish an optimal cryopreservation method for SSCs.MethodsSSCs were obtained from the testes of neonatal male mice aged 3–6 days. Then, 100 μM melatonin was added to the basic freezing medium containing DMSO for cryopreservation of SSCs. Viability, apoptosis-related markers (BAX and BCL2), and intracellular ROS generation level were measured in frozen–thawed SSCs before transplantation using the MTT assay, immunocytochemistry, and flow cytometry, respectively. In addition, Western blotting and immunofluorescence were used to evaluate the expression of proliferation (PLZF and GFRα1) and differentiation (Stra8 and SCP3) proteins in frozen–thawed SSCs after transplantation into recipient testes.ResultsThe data showed that adding melatonin to the cryopreservation medium markedly increased the viability and reduced intracellular ROS generation and apoptosis (by decreasing BAX and increasing BCL2) in the frozen–thawed SSCs (p < 0.05). The expression levels of proliferation (PLZF and GFRα1) and differentiation (Stra8 and SCP3) proteins and resumption of spermatogenesis from frozen–thawed SSCs followed the same pattern after transplantation.ConclusionsThe results of this study revealed that adding melatonin as an antioxidant to the cryopreservation medium containing DMSO could be a promising strategy for cryopreservation of SSCs to maintain fertility in prepubertal male children who suffer from cancer. More... »

PAGES

346

References to SciGraph publications

  • 2021-01-22. Effect of a Freezing Medium Containing Melatonin on Markers of Pre-meiotic and Post-meiotic Spermatogonial Stem Cells (SSCs) After Transplantation in an Azoospermia Mouse Model Due to Testicular Torsion in REPRODUCTIVE SCIENCES
  • 2021-01-04. Caloric restriction in female reproduction: is it beneficial or detrimental? in REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
  • 2017-01-26. Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness in SCIENTIFIC REPORTS
  • 2005-07. Functional MT1 and MT2 melatonin receptors in mammals in ENDOCRINE
  • 2010-06-04. Methods for detection and measurement of hydrogen peroxide inside and outside of cells in MOLECULES AND CELLS
  • 2018-06-20. Oxidative damage and antioxidative indicators in 48 h germinated rice embryos during the vitrification–cryopreservation procedure in PLANT CELL REPORTS
  • 2019-05-03. Combined pre-treatments enhance antioxidant metabolism and improve survival of cryopreserved kiwifruit shoot tips in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2015-01-07. Profiling the circulating miRNAs in mice exposed to gram-positive and gram-negative bacteria by Illumina small RNA deep sequencing in JOURNAL OF BIOMEDICAL SCIENCE
  • 2018-11-21. Co-transplantation of mesenchymal stem cells improves spermatogonial stem cell transplantation efficiency in mice in STEM CELL RESEARCH & THERAPY
  • 2018-10-05. Development and Application of Cryoprotectants in SURVIVAL STRATEGIES IN EXTREME COLD AND DESICCATION
  • 2015-07-24. Radioprotective potential of melatonin against 60Co γ-ray-induced testicular injury in male C57BL/6 mice in JOURNAL OF BIOMEDICAL SCIENCE
  • 2021-06-09. MicroRNA-30a-5p promotes differentiation in neonatal mouse spermatogonial stem cells (SSCs) in REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
  • 2016-04-06. Alteration of spermatogenesis following spermatogonial stem cells transplantation in testicular torsion-detorsion mice in JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
  • 2021-09-07. Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation into Azoospermic Torsion Mouse Model in REPRODUCTIVE SCIENCES
  • 2017-08-29. Melatonin ameliorates restraint stress-induced oxidative stress and apoptosis in testicular cells via NF-κB/iNOS and Nrf2/ HO-1 signaling pathway in SCIENTIFIC REPORTS
  • 2017-10-17. The effects of melatonin on colonization of neonate spermatogonial mouse stem cells in a three-dimensional soft agar culture system in STEM CELL RESEARCH & THERAPY
  • 2017-12-01. Melatonin affects membrane integrity, intracellular reactive oxygen species, caspase3 activity and AKT phosphorylation in frozen thawed human sperm in CELL AND TISSUE RESEARCH
  • 1996-06. Reconstitution of spermatogenesis from frozen spermatogonial stem cells in NATURE MEDICINE
  • 2011-03-23. In vitro production of functional sperm in cultured neonatal mouse testes in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13287-022-03029-1

    DOI

    http://dx.doi.org/10.1186/s13287-022-03029-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1149784311

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/35883101


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1114", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Paediatrics and Reproductive Medicine", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Adult Germline Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Antioxidants", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Azoospermia", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cryopreservation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Dimethyl Sulfoxide", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Freezing", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Male", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Melatonin", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Reactive Oxygen Species", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Spermatogonia", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Testis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "bcl-2-Associated X Protein", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran", 
              "id": "http://www.grid.ac/institutes/grid.411705.6", 
              "name": [
                "Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kazemzadeh", 
            "givenName": "Shokoofeh", 
            "id": "sg:person.016471115757.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016471115757.23"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran", 
              "id": "http://www.grid.ac/institutes/grid.449129.3", 
              "name": [
                "Department of Anatomy, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran", 
                "Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mohammadpour", 
            "givenName": "Shahram", 
            "id": "sg:person.010764754255.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010764754255.04"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran", 
              "id": "http://www.grid.ac/institutes/grid.468130.8", 
              "name": [
                "Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Madadi", 
            "givenName": "Soheila", 
            "id": "sg:person.010461626602.43", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010461626602.43"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran", 
              "id": "http://www.grid.ac/institutes/grid.449129.3", 
              "name": [
                "Department of Anatomy, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran", 
                "Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Babakhani", 
            "givenName": "Azar", 
            "id": "sg:person.01037674605.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037674605.04"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran", 
              "id": "http://www.grid.ac/institutes/grid.411705.6", 
              "name": [
                "Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shabani", 
            "givenName": "Maryam", 
            "id": "sg:person.012137155411.36", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012137155411.36"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran", 
              "id": "http://www.grid.ac/institutes/grid.411036.1", 
              "name": [
                "Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Khanehzad", 
            "givenName": "Maryam", 
            "id": "sg:person.01076645050.91", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076645050.91"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nature09850", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032316133", 
              "https://doi.org/10.1038/nature09850"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12958-021-00758-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1138738901", 
              "https://doi.org/10.1186/s12958-021-00758-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10059-010-0082-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003513567", 
              "https://doi.org/10.1007/s10059-010-0082-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s43032-021-00729-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1140944338", 
              "https://doi.org/10.1007/s43032-021-00729-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11240-019-01617-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1113906470", 
              "https://doi.org/10.1007/s11240-019-01617-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10815-016-0708-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026641660", 
              "https://doi.org/10.1007/s10815-016-0708-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12958-020-00681-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1134304516", 
              "https://doi.org/10.1186/s12958-020-00681-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s43032-020-00447-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1134759487", 
              "https://doi.org/10.1007/s43032-020-00447-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13287-018-1065-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1110097364", 
              "https://doi.org/10.1186/s13287-018-1065-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13287-017-0687-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092249094", 
              "https://doi.org/10.1186/s13287-017-0687-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00441-017-2743-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1093085072", 
              "https://doi.org/10.1007/s00441-017-2743-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12929-014-0106-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013007339", 
              "https://doi.org/10.1186/s12929-014-0106-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00299-018-2315-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1105005401", 
              "https://doi.org/10.1007/s00299-018-2315-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-017-09943-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091313413", 
              "https://doi.org/10.1038/s41598-017-09943-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12929-015-0156-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000593855", 
              "https://doi.org/10.1186/s12929-015-0156-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm0696-693", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021854878", 
              "https://doi.org/10.1038/nm0696-693"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-016-0028-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1079403223", 
              "https://doi.org/10.1038/s41598-016-0028-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1385/endo:27:2:101", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010104011", 
              "https://doi.org/10.1385/endo:27:2:101"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-981-13-1244-1_18", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1107414767", 
              "https://doi.org/10.1007/978-981-13-1244-1_18"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2022-07-26", 
        "datePublishedReg": "2022-07-26", 
        "description": "BackgroundCryostorage of spermatogonial stem cells (SSCs) is an appropriate procedure for long-term storage of SSCs for fertility preservation. However, it causes damage to cellular structures through overproduction of ROS and oxidative stress. In this study, we examined the protective effect of melatonin as a potent antioxidant in the basic freezing medium to establish an optimal cryopreservation method for SSCs.MethodsSSCs were obtained from the testes of neonatal male mice aged 3\u20136\u00a0days. Then, 100\u00a0\u03bcM melatonin was added to the basic freezing medium containing DMSO for cryopreservation of SSCs. Viability, apoptosis-related markers (BAX and BCL2), and intracellular ROS generation level were measured in frozen\u2013thawed SSCs before transplantation using the MTT assay, immunocytochemistry, and flow cytometry, respectively. In addition, Western blotting and immunofluorescence were used to evaluate the expression of proliferation (PLZF and GFR\u03b11) and differentiation (Stra8 and SCP3) proteins in frozen\u2013thawed SSCs after transplantation into recipient testes.ResultsThe data showed that adding melatonin to the cryopreservation medium markedly increased the viability and reduced intracellular ROS generation and apoptosis (by decreasing BAX and increasing BCL2) in the frozen\u2013thawed SSCs (p\u2009<\u20090.05). The expression levels of proliferation (PLZF and GFR\u03b11) and differentiation (Stra8 and SCP3) proteins and resumption of spermatogenesis from frozen\u2013thawed SSCs followed the same pattern after transplantation.ConclusionsThe results of this study revealed that adding melatonin as an antioxidant to the cryopreservation medium containing DMSO could be a promising strategy for cryopreservation of SSCs to maintain fertility in prepubertal male children who suffer from cancer.", 
        "genre": "article", 
        "id": "sg:pub.10.1186/s13287-022-03029-1", 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1043222", 
            "issn": [
              "1757-6512"
            ], 
            "name": "Stem Cell Research & Therapy", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "13"
          }
        ], 
        "keywords": [
          "cryopreservation of SSCs", 
          "spermatogonial stem cells", 
          "prepubertal male children", 
          "neonatal male mice", 
          "expression of proliferation", 
          "apoptosis-related markers", 
          "ROS generation levels", 
          "stem cells", 
          "resumption of spermatogenesis", 
          "fertility preservation", 
          "male mice", 
          "intracellular ROS generation", 
          "protective effect", 
          "male children", 
          "azoospermic mice", 
          "transplantation", 
          "Western blotting", 
          "ResultsThe data", 
          "potent antioxidant", 
          "oxidative stress", 
          "overproduction of ROS", 
          "melatonin", 
          "recipient testes", 
          "transplantation efficiency", 
          "differentiation protein", 
          "MTT assay", 
          "cryopreservation medium", 
          "expression levels", 
          "ROS generation", 
          "ConclusionsThe results", 
          "mice", 
          "testis", 
          "freezing medium", 
          "proliferation", 
          "promising strategy", 
          "antioxidants", 
          "same pattern", 
          "cells", 
          "optimal cryopreservation method", 
          "appropriate procedure", 
          "\u03bcM melatonin", 
          "cancer", 
          "cytometry", 
          "immunocytochemistry", 
          "immunofluorescence", 
          "cryopreservation", 
          "blotting", 
          "levels", 
          "cryopreservation method", 
          "children", 
          "apoptosis", 
          "viability", 
          "markers", 
          "study", 
          "resumption", 
          "days", 
          "spermatogenesis", 
          "assays", 
          "ROS", 
          "overproduction", 
          "expression", 
          "damage", 
          "differentiation", 
          "protein", 
          "fertility", 
          "preservation", 
          "procedure", 
          "effect", 
          "patterns", 
          "stress", 
          "data", 
          "addition", 
          "medium", 
          "strategies", 
          "long-term storage", 
          "DMSO", 
          "results", 
          "cellular structure", 
          "method", 
          "generation", 
          "generation levels", 
          "storage", 
          "structure", 
          "efficiency"
        ], 
        "name": "Melatonin in cryopreservation media improves transplantation efficiency of frozen\u2013thawed spermatogonial stem cells into testes of azoospermic mice", 
        "pagination": "346", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1149784311"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/s13287-022-03029-1"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "35883101"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/s13287-022-03029-1", 
          "https://app.dimensions.ai/details/publication/pub.1149784311"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-09-02T16:07", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/article/article_938.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1186/s13287-022-03029-1"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13287-022-03029-1'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13287-022-03029-1'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13287-022-03029-1'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13287-022-03029-1'


     

    This table displays all metadata directly associated to this object as RDF triples.

    327 TRIPLES      21 PREDICATES      143 URIs      116 LITERALS      22 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/s13287-022-03029-1 schema:about N044cdc4b31c64334b4cb4074813955e9
    2 N0c0d48d100a9447dbe5576ecf3f22f54
    3 N138bced43edc43ab981cc23b7d25bd5d
    4 N3361eff7fb8f4dc584b419128fa594a3
    5 N4cd1485b6dfd44d9a4f1ea62ecbc4b2c
    6 N6144311d816b43c187e21c75139638b9
    7 N6ce2b3296c814d19a212562c1d57594b
    8 N6d7f68ebd98940fc93d4b169189cf1b3
    9 N83ecbb6f61044c8494af858aae54a411
    10 N9634794c44e74709a672fa69a3e82b12
    11 Nc0c7d48b5cb64f91a43251d2946f6b93
    12 Nd26b43b7cfcc4e23803fc8345dfb4e59
    13 Nd5653589880e4f84819202e1e0c251cb
    14 Nd93ea6108b6f47559d0587a3327b0986
    15 Nf5f09e3f30d54f8284ebac9b9e5e1840
    16 anzsrc-for:11
    17 anzsrc-for:1114
    18 schema:author N2da2a6670cb64df09c3deae7a5b88f4c
    19 schema:citation sg:pub.10.1007/978-981-13-1244-1_18
    20 sg:pub.10.1007/s00299-018-2315-4
    21 sg:pub.10.1007/s00441-017-2743-4
    22 sg:pub.10.1007/s10059-010-0082-3
    23 sg:pub.10.1007/s10815-016-0708-2
    24 sg:pub.10.1007/s11240-019-01617-3
    25 sg:pub.10.1007/s43032-020-00447-5
    26 sg:pub.10.1007/s43032-021-00729-6
    27 sg:pub.10.1038/nature09850
    28 sg:pub.10.1038/nm0696-693
    29 sg:pub.10.1038/s41598-016-0028-x
    30 sg:pub.10.1038/s41598-017-09943-2
    31 sg:pub.10.1186/s12929-014-0106-y
    32 sg:pub.10.1186/s12929-015-0156-9
    33 sg:pub.10.1186/s12958-020-00681-1
    34 sg:pub.10.1186/s12958-021-00758-5
    35 sg:pub.10.1186/s13287-017-0687-y
    36 sg:pub.10.1186/s13287-018-1065-0
    37 sg:pub.10.1385/endo:27:2:101
    38 schema:datePublished 2022-07-26
    39 schema:datePublishedReg 2022-07-26
    40 schema:description BackgroundCryostorage of spermatogonial stem cells (SSCs) is an appropriate procedure for long-term storage of SSCs for fertility preservation. However, it causes damage to cellular structures through overproduction of ROS and oxidative stress. In this study, we examined the protective effect of melatonin as a potent antioxidant in the basic freezing medium to establish an optimal cryopreservation method for SSCs.MethodsSSCs were obtained from the testes of neonatal male mice aged 3–6 days. Then, 100 μM melatonin was added to the basic freezing medium containing DMSO for cryopreservation of SSCs. Viability, apoptosis-related markers (BAX and BCL2), and intracellular ROS generation level were measured in frozen–thawed SSCs before transplantation using the MTT assay, immunocytochemistry, and flow cytometry, respectively. In addition, Western blotting and immunofluorescence were used to evaluate the expression of proliferation (PLZF and GFRα1) and differentiation (Stra8 and SCP3) proteins in frozen–thawed SSCs after transplantation into recipient testes.ResultsThe data showed that adding melatonin to the cryopreservation medium markedly increased the viability and reduced intracellular ROS generation and apoptosis (by decreasing BAX and increasing BCL2) in the frozen–thawed SSCs (p < 0.05). The expression levels of proliferation (PLZF and GFRα1) and differentiation (Stra8 and SCP3) proteins and resumption of spermatogenesis from frozen–thawed SSCs followed the same pattern after transplantation.ConclusionsThe results of this study revealed that adding melatonin as an antioxidant to the cryopreservation medium containing DMSO could be a promising strategy for cryopreservation of SSCs to maintain fertility in prepubertal male children who suffer from cancer.
    41 schema:genre article
    42 schema:isAccessibleForFree true
    43 schema:isPartOf N63ffdc910e944ad2bb800b4bc3296b00
    44 N6860f7004eaf4d52b50c7a46da393c20
    45 sg:journal.1043222
    46 schema:keywords ConclusionsThe results
    47 DMSO
    48 MTT assay
    49 ROS
    50 ROS generation
    51 ROS generation levels
    52 ResultsThe data
    53 Western blotting
    54 addition
    55 antioxidants
    56 apoptosis
    57 apoptosis-related markers
    58 appropriate procedure
    59 assays
    60 azoospermic mice
    61 blotting
    62 cancer
    63 cells
    64 cellular structure
    65 children
    66 cryopreservation
    67 cryopreservation medium
    68 cryopreservation method
    69 cryopreservation of SSCs
    70 cytometry
    71 damage
    72 data
    73 days
    74 differentiation
    75 differentiation protein
    76 effect
    77 efficiency
    78 expression
    79 expression levels
    80 expression of proliferation
    81 fertility
    82 fertility preservation
    83 freezing medium
    84 generation
    85 generation levels
    86 immunocytochemistry
    87 immunofluorescence
    88 intracellular ROS generation
    89 levels
    90 long-term storage
    91 male children
    92 male mice
    93 markers
    94 medium
    95 melatonin
    96 method
    97 mice
    98 neonatal male mice
    99 optimal cryopreservation method
    100 overproduction
    101 overproduction of ROS
    102 oxidative stress
    103 patterns
    104 potent antioxidant
    105 prepubertal male children
    106 preservation
    107 procedure
    108 proliferation
    109 promising strategy
    110 protective effect
    111 protein
    112 recipient testes
    113 results
    114 resumption
    115 resumption of spermatogenesis
    116 same pattern
    117 spermatogenesis
    118 spermatogonial stem cells
    119 stem cells
    120 storage
    121 strategies
    122 stress
    123 structure
    124 study
    125 testis
    126 transplantation
    127 transplantation efficiency
    128 viability
    129 μM melatonin
    130 schema:name Melatonin in cryopreservation media improves transplantation efficiency of frozen–thawed spermatogonial stem cells into testes of azoospermic mice
    131 schema:pagination 346
    132 schema:productId N0baeaaf4198142399f59e8b34a63654c
    133 N5730c6952cbb4b598ef773c36aecdcdb
    134 N83329e5cabb546e1bd564443009fc796
    135 schema:sameAs https://app.dimensions.ai/details/publication/pub.1149784311
    136 https://doi.org/10.1186/s13287-022-03029-1
    137 schema:sdDatePublished 2022-09-02T16:07
    138 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    139 schema:sdPublisher N82d09c5eec834ceaa3441c5e4d7102ea
    140 schema:url https://doi.org/10.1186/s13287-022-03029-1
    141 sgo:license sg:explorer/license/
    142 sgo:sdDataset articles
    143 rdf:type schema:ScholarlyArticle
    144 N044cdc4b31c64334b4cb4074813955e9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    145 schema:name Adult Germline Stem Cells
    146 rdf:type schema:DefinedTerm
    147 N0baeaaf4198142399f59e8b34a63654c schema:name dimensions_id
    148 schema:value pub.1149784311
    149 rdf:type schema:PropertyValue
    150 N0c0d48d100a9447dbe5576ecf3f22f54 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    151 schema:name Spermatogonia
    152 rdf:type schema:DefinedTerm
    153 N138bced43edc43ab981cc23b7d25bd5d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    154 schema:name Freezing
    155 rdf:type schema:DefinedTerm
    156 N2da2a6670cb64df09c3deae7a5b88f4c rdf:first sg:person.016471115757.23
    157 rdf:rest Ne9d6e919bb1047bc9ba7fbd63ee5d2f2
    158 N3361eff7fb8f4dc584b419128fa594a3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    159 schema:name Cryopreservation
    160 rdf:type schema:DefinedTerm
    161 N38eed13cbc914bbc839b67e7c9b943c8 rdf:first sg:person.010461626602.43
    162 rdf:rest N593ba7e5ce4e4fc5944823045fd975ea
    163 N4cd1485b6dfd44d9a4f1ea62ecbc4b2c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    164 schema:name Azoospermia
    165 rdf:type schema:DefinedTerm
    166 N5730c6952cbb4b598ef773c36aecdcdb schema:name pubmed_id
    167 schema:value 35883101
    168 rdf:type schema:PropertyValue
    169 N593ba7e5ce4e4fc5944823045fd975ea rdf:first sg:person.01037674605.04
    170 rdf:rest Na2311b09a0a94aa1b30c8986e1df25b7
    171 N6144311d816b43c187e21c75139638b9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    172 schema:name Mice
    173 rdf:type schema:DefinedTerm
    174 N63ffdc910e944ad2bb800b4bc3296b00 schema:volumeNumber 13
    175 rdf:type schema:PublicationVolume
    176 N6860f7004eaf4d52b50c7a46da393c20 schema:issueNumber 1
    177 rdf:type schema:PublicationIssue
    178 N6ce2b3296c814d19a212562c1d57594b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    179 schema:name Testis
    180 rdf:type schema:DefinedTerm
    181 N6d7f68ebd98940fc93d4b169189cf1b3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    182 schema:name Male
    183 rdf:type schema:DefinedTerm
    184 N82d09c5eec834ceaa3441c5e4d7102ea schema:name Springer Nature - SN SciGraph project
    185 rdf:type schema:Organization
    186 N83329e5cabb546e1bd564443009fc796 schema:name doi
    187 schema:value 10.1186/s13287-022-03029-1
    188 rdf:type schema:PropertyValue
    189 N83ecbb6f61044c8494af858aae54a411 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    190 schema:name Reactive Oxygen Species
    191 rdf:type schema:DefinedTerm
    192 N9634794c44e74709a672fa69a3e82b12 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    193 schema:name Antioxidants
    194 rdf:type schema:DefinedTerm
    195 Na2311b09a0a94aa1b30c8986e1df25b7 rdf:first sg:person.012137155411.36
    196 rdf:rest Nb68e93135d524b2da97939fa4d3df918
    197 Nb68e93135d524b2da97939fa4d3df918 rdf:first sg:person.01076645050.91
    198 rdf:rest rdf:nil
    199 Nc0c7d48b5cb64f91a43251d2946f6b93 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    200 schema:name Humans
    201 rdf:type schema:DefinedTerm
    202 Nd26b43b7cfcc4e23803fc8345dfb4e59 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    203 schema:name Animals
    204 rdf:type schema:DefinedTerm
    205 Nd5653589880e4f84819202e1e0c251cb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    206 schema:name Melatonin
    207 rdf:type schema:DefinedTerm
    208 Nd93ea6108b6f47559d0587a3327b0986 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    209 schema:name bcl-2-Associated X Protein
    210 rdf:type schema:DefinedTerm
    211 Ne9d6e919bb1047bc9ba7fbd63ee5d2f2 rdf:first sg:person.010764754255.04
    212 rdf:rest N38eed13cbc914bbc839b67e7c9b943c8
    213 Nf5f09e3f30d54f8284ebac9b9e5e1840 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    214 schema:name Dimethyl Sulfoxide
    215 rdf:type schema:DefinedTerm
    216 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    217 schema:name Medical and Health Sciences
    218 rdf:type schema:DefinedTerm
    219 anzsrc-for:1114 schema:inDefinedTermSet anzsrc-for:
    220 schema:name Paediatrics and Reproductive Medicine
    221 rdf:type schema:DefinedTerm
    222 sg:journal.1043222 schema:issn 1757-6512
    223 schema:name Stem Cell Research & Therapy
    224 schema:publisher Springer Nature
    225 rdf:type schema:Periodical
    226 sg:person.01037674605.04 schema:affiliation grid-institutes:grid.449129.3
    227 schema:familyName Babakhani
    228 schema:givenName Azar
    229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037674605.04
    230 rdf:type schema:Person
    231 sg:person.010461626602.43 schema:affiliation grid-institutes:grid.468130.8
    232 schema:familyName Madadi
    233 schema:givenName Soheila
    234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010461626602.43
    235 rdf:type schema:Person
    236 sg:person.010764754255.04 schema:affiliation grid-institutes:grid.449129.3
    237 schema:familyName Mohammadpour
    238 schema:givenName Shahram
    239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010764754255.04
    240 rdf:type schema:Person
    241 sg:person.01076645050.91 schema:affiliation grid-institutes:grid.411036.1
    242 schema:familyName Khanehzad
    243 schema:givenName Maryam
    244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076645050.91
    245 rdf:type schema:Person
    246 sg:person.012137155411.36 schema:affiliation grid-institutes:grid.411705.6
    247 schema:familyName Shabani
    248 schema:givenName Maryam
    249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012137155411.36
    250 rdf:type schema:Person
    251 sg:person.016471115757.23 schema:affiliation grid-institutes:grid.411705.6
    252 schema:familyName Kazemzadeh
    253 schema:givenName Shokoofeh
    254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016471115757.23
    255 rdf:type schema:Person
    256 sg:pub.10.1007/978-981-13-1244-1_18 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107414767
    257 https://doi.org/10.1007/978-981-13-1244-1_18
    258 rdf:type schema:CreativeWork
    259 sg:pub.10.1007/s00299-018-2315-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105005401
    260 https://doi.org/10.1007/s00299-018-2315-4
    261 rdf:type schema:CreativeWork
    262 sg:pub.10.1007/s00441-017-2743-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1093085072
    263 https://doi.org/10.1007/s00441-017-2743-4
    264 rdf:type schema:CreativeWork
    265 sg:pub.10.1007/s10059-010-0082-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003513567
    266 https://doi.org/10.1007/s10059-010-0082-3
    267 rdf:type schema:CreativeWork
    268 sg:pub.10.1007/s10815-016-0708-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026641660
    269 https://doi.org/10.1007/s10815-016-0708-2
    270 rdf:type schema:CreativeWork
    271 sg:pub.10.1007/s11240-019-01617-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1113906470
    272 https://doi.org/10.1007/s11240-019-01617-3
    273 rdf:type schema:CreativeWork
    274 sg:pub.10.1007/s43032-020-00447-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1134759487
    275 https://doi.org/10.1007/s43032-020-00447-5
    276 rdf:type schema:CreativeWork
    277 sg:pub.10.1007/s43032-021-00729-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1140944338
    278 https://doi.org/10.1007/s43032-021-00729-6
    279 rdf:type schema:CreativeWork
    280 sg:pub.10.1038/nature09850 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032316133
    281 https://doi.org/10.1038/nature09850
    282 rdf:type schema:CreativeWork
    283 sg:pub.10.1038/nm0696-693 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021854878
    284 https://doi.org/10.1038/nm0696-693
    285 rdf:type schema:CreativeWork
    286 sg:pub.10.1038/s41598-016-0028-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079403223
    287 https://doi.org/10.1038/s41598-016-0028-x
    288 rdf:type schema:CreativeWork
    289 sg:pub.10.1038/s41598-017-09943-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091313413
    290 https://doi.org/10.1038/s41598-017-09943-2
    291 rdf:type schema:CreativeWork
    292 sg:pub.10.1186/s12929-014-0106-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1013007339
    293 https://doi.org/10.1186/s12929-014-0106-y
    294 rdf:type schema:CreativeWork
    295 sg:pub.10.1186/s12929-015-0156-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000593855
    296 https://doi.org/10.1186/s12929-015-0156-9
    297 rdf:type schema:CreativeWork
    298 sg:pub.10.1186/s12958-020-00681-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1134304516
    299 https://doi.org/10.1186/s12958-020-00681-1
    300 rdf:type schema:CreativeWork
    301 sg:pub.10.1186/s12958-021-00758-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1138738901
    302 https://doi.org/10.1186/s12958-021-00758-5
    303 rdf:type schema:CreativeWork
    304 sg:pub.10.1186/s13287-017-0687-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1092249094
    305 https://doi.org/10.1186/s13287-017-0687-y
    306 rdf:type schema:CreativeWork
    307 sg:pub.10.1186/s13287-018-1065-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1110097364
    308 https://doi.org/10.1186/s13287-018-1065-0
    309 rdf:type schema:CreativeWork
    310 sg:pub.10.1385/endo:27:2:101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010104011
    311 https://doi.org/10.1385/endo:27:2:101
    312 rdf:type schema:CreativeWork
    313 grid-institutes:grid.411036.1 schema:alternateName Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
    314 schema:name Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
    315 rdf:type schema:Organization
    316 grid-institutes:grid.411705.6 schema:alternateName Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
    317 Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
    318 schema:name Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
    319 Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
    320 rdf:type schema:Organization
    321 grid-institutes:grid.449129.3 schema:alternateName Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran
    322 schema:name Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran
    323 Department of Anatomy, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran
    324 rdf:type schema:Organization
    325 grid-institutes:grid.468130.8 schema:alternateName Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran
    326 schema:name Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran
    327 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...