The therapeutic effect of stem cells from human exfoliated deciduous teeth on a rat model of tracheal fistula View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2022-07-15

AUTHORS

Fang Wang, Zhangwen Li, Feng-Juan Lyu, Jie Gao, Jinle Lin, Jianling Liu, Xiaowen Chen, Zhongpeng Li, Jiajie Shan, Jian Wu

ABSTRACT

BackgroundTracheal fistulas (TF) can be dangerous and even fatal in patients. The current treatment is really challenging. Previous studies reported that mesenchymal stem cells (MSCs) could be used to treat respiratory tract fistulas. Stem cells from human exfoliated deciduous teeth (SHED) are considered to be MSC-like cells that may also have the potential to treat the tracheal fistulas. In this study, we investigated the therapeutic effects of SHED in rat tracheal fistula models.MethodsA total of 80 SD rats were randomly divided into five groups: a sham-operated group, a local PBS group (L-PBS), an intravenous PBS group (I-PBS), a local SHED treatment group (L-SHED), and an intravenous SHED treatment group (I-SHED). The L-SHED and I-SHED groups were given a topical application around the fistula or an intravenous injection of 1*107 SHED via the tail vein, respectively, while the L-PBS and I-PBS groups were given an equivalent volume of PBS through local or intravenous administration. A stereomicroscope was used to observe fistula healing on the 2nd, 3rd, and 5th days following transplantation. On the 7th day, the survival of SHED was observed by immunofluorescence. The pathology of the lungs and fistulas was observed by hematoxylin and eosin (H&E) and Masson staining. The expression levels of the Toll-like receptor 4 (TLR4), interleukin (IL)-1β, IL-33, and IL-4 were measured using immunohistochemistry. The expression levels of TLR4, high mobility group box 1 (HMGB1), and myeloid differentiation factor 88 (MYD88) were studied using western blotting. On day 14, airway responsiveness of rats was detected and analyzed.ResultsFistula healing in the L-SHED and I-SHED groups was faster than that in their respective PBS groups after transplantation. The fistula diameters in the L-SHED and I-SHED groups were significantly smaller than those in the L-PBS and I-PBS groups on the 3rd day. Moreover, the phenomenon of fibroblast proliferation and new blood vessel growth around the fistula seemed more pronounced in the L-SHED and I-SHED groups. Although no discernible difference was found in airway responsiveness after SHED treatment, the degree of inflammation in the lungs was reduced by intravenous SHED treatment. However, there was no significant reduction in lung inflammation by local SHED treatment. The expression levels of IL-1β and IL-33 were decreased in the I-SHED group, while IL-4 was elevated compared with the I-PBS group. Interestingly, intravenous SHED treatment inhibited the activation of HMGB1/TLR4/MYD88 in the lung tissues of TF rats.ConclusionsSHED transplantation accelerated the rate of fistula healing in rats. Intravenous SHED treatment reduced lung inflammation. Thus, SHED may have potential in the treatment of tracheal fistula, providing hope for future therapeutic development for TF. More... »

PAGES

310

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13287-022-02994-x

DOI

http://dx.doi.org/10.1186/s13287-022-02994-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1149508667

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/35841116


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1102", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Cardiorespiratory Medicine and Haematology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HMGB1 Protein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Interleukin-33", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Interleukin-4", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Myeloid Differentiation Factor 88", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats, Sprague-Dawley", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Respiratory Tract Fistula", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Toll-Like Receptor 4", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tooth, Deciduous", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "School of Medicine, South China University of Technology, 510006, Guangzhou, China", 
            "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Fang", 
        "id": "sg:person.011460243307.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011460243307.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "School of Medicine, South China University of Technology, 510006, Guangzhou, China", 
            "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Zhangwen", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Medicine, South China University of Technology, 510006, Guangzhou, China", 
          "id": "http://www.grid.ac/institutes/grid.79703.3a", 
          "name": [
            "School of Medicine, South China University of Technology, 510006, Guangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lyu", 
        "givenName": "Feng-Juan", 
        "id": "sg:person.010005236243.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010005236243.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "The Second School of Clinical Medicine, Southern Medical University, 510515, Guangzhou, China", 
            "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gao", 
        "givenName": "Jie", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Emergency Medicine, Affiliated Baoan Hospital of Shenzhen, The second school of clinical medicine, Southern Medical University, 518101, Shenzhen, China", 
          "id": "http://www.grid.ac/institutes/grid.284723.8", 
          "name": [
            "The Second School of Clinical Medicine, Southern Medical University, 510515, Guangzhou, China", 
            "Department of Emergency Medicine, Affiliated Baoan Hospital of Shenzhen, The second school of clinical medicine, Southern Medical University, 518101, Shenzhen, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lin", 
        "givenName": "Jinle", 
        "id": "sg:person.011114345413.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011114345413.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "School of Medicine, South China University of Technology, 510006, Guangzhou, China", 
            "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Jianling", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "School of Medicine, South China University of Technology, 510006, Guangzhou, China", 
            "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "Xiaowen", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "The Second School of Clinical Medicine, Southern Medical University, 510515, Guangzhou, China", 
            "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Zhongpeng", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Medicine, South China University of Technology, 510006, Guangzhou, China", 
          "id": "http://www.grid.ac/institutes/grid.79703.3a", 
          "name": [
            "School of Medicine, South China University of Technology, 510006, Guangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shan", 
        "givenName": "Jiajie", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "School of Medicine, South China University of Technology, 510006, Guangzhou, China", 
            "Second Department of Elderly Respiratory, Guangdong Provincial People\u2019s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wu", 
        "givenName": "Jian", 
        "id": "sg:person.012050231127.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012050231127.24"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1186/s13287-017-0760-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100381874", 
          "https://doi.org/10.1186/s13287-017-0760-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12890-020-01372-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1134384808", 
          "https://doi.org/10.1186/s12890-020-01372-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri2395", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034703692", 
          "https://doi.org/10.1038/nri2395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13019-018-0799-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1109910647", 
          "https://doi.org/10.1186/s13019-018-0799-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-018-31414-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1106343733", 
          "https://doi.org/10.1038/s41598-018-31414-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002700010071", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030094948", 
          "https://doi.org/10.1007/s002700010071"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13287-015-0154-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036636774", 
          "https://doi.org/10.1186/s13287-015-0154-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13287-020-01968-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1132011998", 
          "https://doi.org/10.1186/s13287-020-01968-1"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2022-07-15", 
    "datePublishedReg": "2022-07-15", 
    "description": "BackgroundTracheal fistulas (TF) can be dangerous and even fatal in patients. The current treatment is really challenging. Previous studies reported that mesenchymal stem cells (MSCs) could be used to treat respiratory tract fistulas.\u00a0Stem cells from human exfoliated deciduous teeth (SHED) are considered to be MSC-like cells that may also have the potential to treat the tracheal fistulas. In this study, we investigated the therapeutic effects of SHED in rat tracheal fistula models.MethodsA total of 80 SD rats were randomly divided into five groups: a sham-operated group, a local PBS group (L-PBS), an intravenous PBS group (I-PBS), a local SHED treatment group (L-SHED), and an intravenous SHED treatment group (I-SHED). The L-SHED and I-SHED groups were given a topical application around the fistula or an intravenous injection of 1*107 SHED via the tail vein, respectively, while the L-PBS and I-PBS groups were given an equivalent volume of PBS through local or intravenous administration. A stereomicroscope was used to observe fistula healing on the 2nd, 3rd, and 5th days following transplantation. On the 7th day, the survival of SHED was observed by immunofluorescence. The pathology of the lungs and fistulas was observed by hematoxylin and eosin (H&E) and Masson staining. The expression levels of the Toll-like receptor 4 (TLR4), interleukin (IL)-1\u03b2, IL-33, and IL-4 were measured using immunohistochemistry. The expression levels of TLR4, high mobility group box 1 (HMGB1), and myeloid differentiation factor 88 (MYD88) were studied using western blotting. On day 14, airway responsiveness of rats was detected and analyzed.ResultsFistula healing in the L-SHED and I-SHED groups was faster than that in their respective PBS groups after transplantation. The fistula diameters in the L-SHED and I-SHED groups were significantly smaller than those in the L-PBS and I-PBS groups on the 3rd day. Moreover, the phenomenon of fibroblast proliferation and new blood vessel growth around the fistula seemed more pronounced in the L-SHED and I-SHED groups. Although no discernible difference was found in airway responsiveness after SHED treatment, the degree of inflammation in the lungs was reduced by intravenous SHED treatment. However, there was no significant reduction in lung inflammation by local SHED treatment. The expression levels of IL-1\u03b2 and IL-33 were decreased in the I-SHED group, while IL-4 was elevated compared with the I-PBS group. Interestingly, intravenous SHED treatment inhibited the activation of HMGB1/TLR4/MYD88 in the lung tissues of TF rats.ConclusionsSHED transplantation accelerated the rate of fistula healing in rats. Intravenous SHED treatment reduced lung inflammation. Thus, SHED may have potential in the treatment of tracheal fistula, providing hope for future therapeutic development for TF.", 
    "genre": "article", 
    "id": "sg:pub.10.1186/s13287-022-02994-x", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.8886604", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1043222", 
        "issn": [
          "1757-6512"
        ], 
        "name": "Stem Cell Research & Therapy", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "13"
      }
    ], 
    "keywords": [
      "Toll-like receptor 4", 
      "high mobility group box 1", 
      "PBS group", 
      "tracheal fistula", 
      "airway responsiveness", 
      "lung inflammation", 
      "IL-33", 
      "IL-4", 
      "therapeutic effect", 
      "treatment groups", 
      "deciduous teeth", 
      "mesenchymal stem cells", 
      "myeloid differentiation factor 88", 
      "expression levels", 
      "mobility group box 1", 
      "respiratory tract fistula", 
      "TLR4/MyD88", 
      "sham-operated group", 
      "differentiation factor 88", 
      "degree of inflammation", 
      "rate of fistula", 
      "stem cells", 
      "group box 1", 
      "new blood vessel growth", 
      "future therapeutic development", 
      "fistula diameter", 
      "blood vessel growth", 
      "factor 88", 
      "MSC-like cells", 
      "IL-1\u03b2", 
      "SD rats", 
      "receptor 4", 
      "lung tissue", 
      "rat model", 
      "current treatment", 
      "intravenous administration", 
      "intravenous injection", 
      "tail vein", 
      "fistula model", 
      "fistula", 
      "box 1", 
      "topical application", 
      "day 14", 
      "inflammation", 
      "rats", 
      "transplantation", 
      "fibroblast proliferation", 
      "Western blotting", 
      "equivalent volume", 
      "vessel growth", 
      "therapeutic development", 
      "L PBS", 
      "treatment", 
      "lung", 
      "significant reduction", 
      "days", 
      "group", 
      "cells", 
      "teeth", 
      "responsiveness", 
      "previous studies", 
      "patients", 
      "interleukin", 
      "MyD88", 
      "immunohistochemistry", 
      "levels", 
      "pathology", 
      "hematoxylin", 
      "administration", 
      "discernible differences", 
      "immunofluorescence", 
      "eosin", 
      "survival", 
      "vein", 
      "blotting", 
      "healing", 
      "total", 
      "injection", 
      "Masson", 
      "proliferation", 
      "tissue", 
      "study", 
      "activation", 
      "shed", 
      "effect", 
      "PBS", 
      "differences", 
      "stereomicroscope", 
      "rate", 
      "potential", 
      "reduction", 
      "volume", 
      "hope", 
      "development", 
      "TF", 
      "degree", 
      "diameter", 
      "model", 
      "growth", 
      "phenomenon", 
      "applications"
    ], 
    "name": "The therapeutic effect of stem cells from human exfoliated deciduous teeth on a rat model of tracheal fistula", 
    "pagination": "310", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1149508667"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s13287-022-02994-x"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "35841116"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s13287-022-02994-x", 
      "https://app.dimensions.ai/details/publication/pub.1149508667"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-09-02T16:08", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/article/article_950.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/s13287-022-02994-x"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13287-022-02994-x'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13287-022-02994-x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13287-022-02994-x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13287-022-02994-x'


 

This table displays all metadata directly associated to this object as RDF triples.

309 TRIPLES      21 PREDICATES      146 URIs      130 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s13287-022-02994-x schema:about N46953582220f43d08a1f5aed26fdc1d6
2 N68df23c3fe694694af19aea3a9b70d0d
3 N69f04e0807e24df1ac3ce252579540c1
4 N7dfe082b83fc4dc189fd15c1c5520520
5 N88b793cd924e42dfac0fb7b1fe373323
6 Nb5d1eaf62a874e35bab09143ea969ff6
7 Nb8cfb5fc205240bd8a64eafd0c894d2f
8 Nc9c93e542a6147c089a107e74cedd33f
9 Ncba418ca7c2840dda0a7138330f5b79d
10 Ne60810e058304696aa882e7ac0b9169f
11 Nf58361c58d9d40c3a727fb8b07bd7ba2
12 Nfe05dd6bc3c9425b916dd22e59780125
13 anzsrc-for:11
14 anzsrc-for:1102
15 schema:author N96067886dce04c52a342a43af10aec97
16 schema:citation sg:pub.10.1007/s002700010071
17 sg:pub.10.1038/nri2395
18 sg:pub.10.1038/s41598-018-31414-5
19 sg:pub.10.1186/s12890-020-01372-8
20 sg:pub.10.1186/s13019-018-0799-1
21 sg:pub.10.1186/s13287-015-0154-6
22 sg:pub.10.1186/s13287-017-0760-6
23 sg:pub.10.1186/s13287-020-01968-1
24 schema:datePublished 2022-07-15
25 schema:datePublishedReg 2022-07-15
26 schema:description BackgroundTracheal fistulas (TF) can be dangerous and even fatal in patients. The current treatment is really challenging. Previous studies reported that mesenchymal stem cells (MSCs) could be used to treat respiratory tract fistulas. Stem cells from human exfoliated deciduous teeth (SHED) are considered to be MSC-like cells that may also have the potential to treat the tracheal fistulas. In this study, we investigated the therapeutic effects of SHED in rat tracheal fistula models.MethodsA total of 80 SD rats were randomly divided into five groups: a sham-operated group, a local PBS group (L-PBS), an intravenous PBS group (I-PBS), a local SHED treatment group (L-SHED), and an intravenous SHED treatment group (I-SHED). The L-SHED and I-SHED groups were given a topical application around the fistula or an intravenous injection of 1*107 SHED via the tail vein, respectively, while the L-PBS and I-PBS groups were given an equivalent volume of PBS through local or intravenous administration. A stereomicroscope was used to observe fistula healing on the 2nd, 3rd, and 5th days following transplantation. On the 7th day, the survival of SHED was observed by immunofluorescence. The pathology of the lungs and fistulas was observed by hematoxylin and eosin (H&E) and Masson staining. The expression levels of the Toll-like receptor 4 (TLR4), interleukin (IL)-1β, IL-33, and IL-4 were measured using immunohistochemistry. The expression levels of TLR4, high mobility group box 1 (HMGB1), and myeloid differentiation factor 88 (MYD88) were studied using western blotting. On day 14, airway responsiveness of rats was detected and analyzed.ResultsFistula healing in the L-SHED and I-SHED groups was faster than that in their respective PBS groups after transplantation. The fistula diameters in the L-SHED and I-SHED groups were significantly smaller than those in the L-PBS and I-PBS groups on the 3rd day. Moreover, the phenomenon of fibroblast proliferation and new blood vessel growth around the fistula seemed more pronounced in the L-SHED and I-SHED groups. Although no discernible difference was found in airway responsiveness after SHED treatment, the degree of inflammation in the lungs was reduced by intravenous SHED treatment. However, there was no significant reduction in lung inflammation by local SHED treatment. The expression levels of IL-1β and IL-33 were decreased in the I-SHED group, while IL-4 was elevated compared with the I-PBS group. Interestingly, intravenous SHED treatment inhibited the activation of HMGB1/TLR4/MYD88 in the lung tissues of TF rats.ConclusionsSHED transplantation accelerated the rate of fistula healing in rats. Intravenous SHED treatment reduced lung inflammation. Thus, SHED may have potential in the treatment of tracheal fistula, providing hope for future therapeutic development for TF.
27 schema:genre article
28 schema:isAccessibleForFree true
29 schema:isPartOf N280da067fb804fc3a86075b489bb6174
30 Nb6bacce5e9644cd5a4bc8f1fbc48090f
31 sg:journal.1043222
32 schema:keywords IL-1β
33 IL-33
34 IL-4
35 L PBS
36 MSC-like cells
37 Masson
38 MyD88
39 PBS
40 PBS group
41 SD rats
42 TF
43 TLR4/MyD88
44 Toll-like receptor 4
45 Western blotting
46 activation
47 administration
48 airway responsiveness
49 applications
50 blood vessel growth
51 blotting
52 box 1
53 cells
54 current treatment
55 day 14
56 days
57 deciduous teeth
58 degree
59 degree of inflammation
60 development
61 diameter
62 differences
63 differentiation factor 88
64 discernible differences
65 effect
66 eosin
67 equivalent volume
68 expression levels
69 factor 88
70 fibroblast proliferation
71 fistula
72 fistula diameter
73 fistula model
74 future therapeutic development
75 group
76 group box 1
77 growth
78 healing
79 hematoxylin
80 high mobility group box 1
81 hope
82 immunofluorescence
83 immunohistochemistry
84 inflammation
85 injection
86 interleukin
87 intravenous administration
88 intravenous injection
89 levels
90 lung
91 lung inflammation
92 lung tissue
93 mesenchymal stem cells
94 mobility group box 1
95 model
96 myeloid differentiation factor 88
97 new blood vessel growth
98 pathology
99 patients
100 phenomenon
101 potential
102 previous studies
103 proliferation
104 rat model
105 rate
106 rate of fistula
107 rats
108 receptor 4
109 reduction
110 respiratory tract fistula
111 responsiveness
112 sham-operated group
113 shed
114 significant reduction
115 stem cells
116 stereomicroscope
117 study
118 survival
119 tail vein
120 teeth
121 therapeutic development
122 therapeutic effect
123 tissue
124 topical application
125 total
126 tracheal fistula
127 transplantation
128 treatment
129 treatment groups
130 vein
131 vessel growth
132 volume
133 schema:name The therapeutic effect of stem cells from human exfoliated deciduous teeth on a rat model of tracheal fistula
134 schema:pagination 310
135 schema:productId N87b11ab939ab4f6089492ad27bf4058e
136 Nd2d422dadfcd446ea53aae2b173daa82
137 Ne679eec8ca1b46029511d48e44d194c0
138 schema:sameAs https://app.dimensions.ai/details/publication/pub.1149508667
139 https://doi.org/10.1186/s13287-022-02994-x
140 schema:sdDatePublished 2022-09-02T16:08
141 schema:sdLicense https://scigraph.springernature.com/explorer/license/
142 schema:sdPublisher Na9e982f9dc66480bb3a2ba44f3054299
143 schema:url https://doi.org/10.1186/s13287-022-02994-x
144 sgo:license sg:explorer/license/
145 sgo:sdDataset articles
146 rdf:type schema:ScholarlyArticle
147 N280da067fb804fc3a86075b489bb6174 schema:volumeNumber 13
148 rdf:type schema:PublicationVolume
149 N32fc082726c54c7390265976d291a421 rdf:first N7c5eb11a1541492c9e8d4560df370527
150 rdf:rest N50ecdf3fc2f44d7c88fff8b1308eccb3
151 N4227a99d9c264389b27438e7484aefc6 rdf:first N4d2ff23e828442f3a900b5e6212317b7
152 rdf:rest Nd59d389b78904d8ca44bb657842b3345
153 N46953582220f43d08a1f5aed26fdc1d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Interleukin-4
155 rdf:type schema:DefinedTerm
156 N4d2ff23e828442f3a900b5e6212317b7 schema:affiliation grid-institutes:None
157 schema:familyName Gao
158 schema:givenName Jie
159 rdf:type schema:Person
160 N50c215151f59480caded98241e1235ad rdf:first sg:person.012050231127.24
161 rdf:rest rdf:nil
162 N50ecdf3fc2f44d7c88fff8b1308eccb3 rdf:first Neb855698effc4deba5276369f4540aca
163 rdf:rest N50c215151f59480caded98241e1235ad
164 N68df23c3fe694694af19aea3a9b70d0d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Humans
166 rdf:type schema:DefinedTerm
167 N69f04e0807e24df1ac3ce252579540c1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Respiratory Tract Fistula
169 rdf:type schema:DefinedTerm
170 N6c62915a609445c5a2f8c6a16543c69a schema:affiliation grid-institutes:None
171 schema:familyName Chen
172 schema:givenName Xiaowen
173 rdf:type schema:Person
174 N6fcd520ff48942be918a10c106d9b765 rdf:first Nc76ca58f27354c2ea8c3a2b4c2561bb0
175 rdf:rest Ne022bf2f4860465fbec4db864649f60e
176 N7c5eb11a1541492c9e8d4560df370527 schema:affiliation grid-institutes:None
177 schema:familyName Li
178 schema:givenName Zhongpeng
179 rdf:type schema:Person
180 N7dfe082b83fc4dc189fd15c1c5520520 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Stem Cells
182 rdf:type schema:DefinedTerm
183 N87b11ab939ab4f6089492ad27bf4058e schema:name dimensions_id
184 schema:value pub.1149508667
185 rdf:type schema:PropertyValue
186 N88b793cd924e42dfac0fb7b1fe373323 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
187 schema:name Rats, Sprague-Dawley
188 rdf:type schema:DefinedTerm
189 N96067886dce04c52a342a43af10aec97 rdf:first sg:person.011460243307.00
190 rdf:rest Nf1cc0f5a652046cd8192c956395df749
191 Na9e982f9dc66480bb3a2ba44f3054299 schema:name Springer Nature - SN SciGraph project
192 rdf:type schema:Organization
193 Nb5d1eaf62a874e35bab09143ea969ff6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
194 schema:name Animals
195 rdf:type schema:DefinedTerm
196 Nb6bacce5e9644cd5a4bc8f1fbc48090f schema:issueNumber 1
197 rdf:type schema:PublicationIssue
198 Nb8cfb5fc205240bd8a64eafd0c894d2f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
199 schema:name Tooth, Deciduous
200 rdf:type schema:DefinedTerm
201 Nbfa61f21119a4b0191560284f7b086d6 rdf:first sg:person.010005236243.33
202 rdf:rest N4227a99d9c264389b27438e7484aefc6
203 Nc0335b0d3e964a7898928bc8a4cf17b1 schema:affiliation grid-institutes:None
204 schema:familyName Li
205 schema:givenName Zhangwen
206 rdf:type schema:Person
207 Nc76ca58f27354c2ea8c3a2b4c2561bb0 schema:affiliation grid-institutes:None
208 schema:familyName Liu
209 schema:givenName Jianling
210 rdf:type schema:Person
211 Nc9c93e542a6147c089a107e74cedd33f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
212 schema:name Rats
213 rdf:type schema:DefinedTerm
214 Ncba418ca7c2840dda0a7138330f5b79d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
215 schema:name Myeloid Differentiation Factor 88
216 rdf:type schema:DefinedTerm
217 Nd2d422dadfcd446ea53aae2b173daa82 schema:name pubmed_id
218 schema:value 35841116
219 rdf:type schema:PropertyValue
220 Nd59d389b78904d8ca44bb657842b3345 rdf:first sg:person.011114345413.61
221 rdf:rest N6fcd520ff48942be918a10c106d9b765
222 Ne022bf2f4860465fbec4db864649f60e rdf:first N6c62915a609445c5a2f8c6a16543c69a
223 rdf:rest N32fc082726c54c7390265976d291a421
224 Ne60810e058304696aa882e7ac0b9169f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
225 schema:name Toll-Like Receptor 4
226 rdf:type schema:DefinedTerm
227 Ne679eec8ca1b46029511d48e44d194c0 schema:name doi
228 schema:value 10.1186/s13287-022-02994-x
229 rdf:type schema:PropertyValue
230 Neb855698effc4deba5276369f4540aca schema:affiliation grid-institutes:grid.79703.3a
231 schema:familyName Shan
232 schema:givenName Jiajie
233 rdf:type schema:Person
234 Nf1cc0f5a652046cd8192c956395df749 rdf:first Nc0335b0d3e964a7898928bc8a4cf17b1
235 rdf:rest Nbfa61f21119a4b0191560284f7b086d6
236 Nf58361c58d9d40c3a727fb8b07bd7ba2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
237 schema:name HMGB1 Protein
238 rdf:type schema:DefinedTerm
239 Nfe05dd6bc3c9425b916dd22e59780125 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
240 schema:name Interleukin-33
241 rdf:type schema:DefinedTerm
242 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
243 schema:name Medical and Health Sciences
244 rdf:type schema:DefinedTerm
245 anzsrc-for:1102 schema:inDefinedTermSet anzsrc-for:
246 schema:name Cardiorespiratory Medicine and Haematology
247 rdf:type schema:DefinedTerm
248 sg:grant.8886604 http://pending.schema.org/fundedItem sg:pub.10.1186/s13287-022-02994-x
249 rdf:type schema:MonetaryGrant
250 sg:journal.1043222 schema:issn 1757-6512
251 schema:name Stem Cell Research & Therapy
252 schema:publisher Springer Nature
253 rdf:type schema:Periodical
254 sg:person.010005236243.33 schema:affiliation grid-institutes:grid.79703.3a
255 schema:familyName Lyu
256 schema:givenName Feng-Juan
257 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010005236243.33
258 rdf:type schema:Person
259 sg:person.011114345413.61 schema:affiliation grid-institutes:grid.284723.8
260 schema:familyName Lin
261 schema:givenName Jinle
262 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011114345413.61
263 rdf:type schema:Person
264 sg:person.011460243307.00 schema:affiliation grid-institutes:None
265 schema:familyName Wang
266 schema:givenName Fang
267 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011460243307.00
268 rdf:type schema:Person
269 sg:person.012050231127.24 schema:affiliation grid-institutes:None
270 schema:familyName Wu
271 schema:givenName Jian
272 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012050231127.24
273 rdf:type schema:Person
274 sg:pub.10.1007/s002700010071 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030094948
275 https://doi.org/10.1007/s002700010071
276 rdf:type schema:CreativeWork
277 sg:pub.10.1038/nri2395 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034703692
278 https://doi.org/10.1038/nri2395
279 rdf:type schema:CreativeWork
280 sg:pub.10.1038/s41598-018-31414-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1106343733
281 https://doi.org/10.1038/s41598-018-31414-5
282 rdf:type schema:CreativeWork
283 sg:pub.10.1186/s12890-020-01372-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1134384808
284 https://doi.org/10.1186/s12890-020-01372-8
285 rdf:type schema:CreativeWork
286 sg:pub.10.1186/s13019-018-0799-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1109910647
287 https://doi.org/10.1186/s13019-018-0799-1
288 rdf:type schema:CreativeWork
289 sg:pub.10.1186/s13287-015-0154-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036636774
290 https://doi.org/10.1186/s13287-015-0154-6
291 rdf:type schema:CreativeWork
292 sg:pub.10.1186/s13287-017-0760-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100381874
293 https://doi.org/10.1186/s13287-017-0760-6
294 rdf:type schema:CreativeWork
295 sg:pub.10.1186/s13287-020-01968-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1132011998
296 https://doi.org/10.1186/s13287-020-01968-1
297 rdf:type schema:CreativeWork
298 grid-institutes:None schema:alternateName Second Department of Elderly Respiratory, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China
299 schema:name School of Medicine, South China University of Technology, 510006, Guangzhou, China
300 Second Department of Elderly Respiratory, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Geriatrics Institute, 510080, Guangzhou, China
301 The Second School of Clinical Medicine, Southern Medical University, 510515, Guangzhou, China
302 rdf:type schema:Organization
303 grid-institutes:grid.284723.8 schema:alternateName Department of Emergency Medicine, Affiliated Baoan Hospital of Shenzhen, The second school of clinical medicine, Southern Medical University, 518101, Shenzhen, China
304 schema:name Department of Emergency Medicine, Affiliated Baoan Hospital of Shenzhen, The second school of clinical medicine, Southern Medical University, 518101, Shenzhen, China
305 The Second School of Clinical Medicine, Southern Medical University, 510515, Guangzhou, China
306 rdf:type schema:Organization
307 grid-institutes:grid.79703.3a schema:alternateName School of Medicine, South China University of Technology, 510006, Guangzhou, China
308 schema:name School of Medicine, South China University of Technology, 510006, Guangzhou, China
309 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...