Insights into the roles of sperm in animal cloning View Full Text


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Article Info

DATE

2020-02-18

AUTHORS

Pengxiang Qu, Yongsheng Wang, Chengsheng Zhang, Enqi Liu

ABSTRACT

Somatic cell nuclear transfer (SCNT) has shown a wide application in the generation of transgenic animals, protection of endangered animals, and therapeutic cloning. However, the efficiency of SCNT remains very low due to some poorly characterized key factors. Compared with fertilized embryos, somatic donor cells lack some important components of sperm, such as sperm small noncoding RNA (sncRNA) and proteins. Loss of these factors is considered an important reason for the abnormal development of SCNT embryo. This study focused on recent advances of SCNT and the roles of sperm in development. Sperm-derived factors play an important role in nucleus reprogramming and cytoskeleton remodeling during SCNT embryo development. Hence, considering the role of sperm may provide a new strategy for improving cloning efficiency. More... »

PAGES

65

References to SciGraph publications

  • 2006-05. RNA-mediated non-mendelian inheritance of an epigenetic change in the mouse in NATURE
  • 2017-11-10. Choosing a culture medium for SCNT and iSCNT reconstructed embryos: from domestic to wildlife species in JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
  • 2019-08-06. Successful cloning of a superior buffalo bull in SCIENTIFIC REPORTS
  • 2005-08-03. Dogs cloned from adult somatic cells in NATURE
  • 2016-10-06. Remodeling somatic nuclei via exogenous expression of protamine 1 to create spermatid-like structures for somatic nuclear transfer in NATURE PROTOCOLS
  • 2002-04. Cloned rabbits produced by nuclear transfer from adult somatic cells in NATURE BIOTECHNOLOGY
  • 2003-08. A cloned horse born to its dam twin in NATURE
  • 1998-07-23. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei in NATURE
  • 1997-02. Viable offspring derived from fetal and adult mammalian cells in NATURE
  • 2000-09. Cloned pigs produced by nuclear transfer from adult somatic cells in NATURE
  • 1999-05. Production of goats by somatic cell nuclear transfer in NATURE BIOTECHNOLOGY
  • 2002-02-14. A cat cloned by nuclear transplantation in NATURE
  • 2001-10. Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells in NATURE BIOTECHNOLOGY
  • 2016-02-03. Gene targeting, genome editing: from Dolly to editors in TRANSGENIC RESEARCH
  • 2018-07-09. Cold-induced epigenetic programming of the sperm enhances brown adipose tissue activity in the offspring in NATURE MEDICINE
  • 2011-05-18. Global quantification of mammalian gene expression control in NATURE
  • 2014-05-24. Posttranslationally Modified Tubulins and Other Cytoskeletal Proteins: Their Role in Gametogenesis, Oocyte Maturation, Fertilization and Pre-implantation Embryo Development in POSTTRANSLATIONAL PROTEIN MODIFICATIONS IN THE REPRODUCTIVE SYSTEM
  • 2010-10-20. Chronic high-fat diet in fathers programs β-cell dysfunction in female rat offspring in NATURE
  • 2015-01-10. Movement of chromosomes with severed kinetochore microtubules in PROTOPLASMA
  • 1996-01. Requirement for Xist in X chromosome inactivation in NATURE
  • 2017-10-17. Sperm-borne miR-449b influences cleavage, epigenetic reprogramming and apoptosis of SCNT embryos in bovine in SCIENTIFIC REPORTS
  • 2014-04-16. Juno is the egg Izumo receptor and is essential for mammalian fertilization in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13287-020-01599-6

    DOI

    http://dx.doi.org/10.1186/s13287-020-01599-6

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/32070430


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