Human umbilical cord stem cell conditioned medium versus serum-free culture medium in the treatment of cryopreserved human ovarian tissues in ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Yingxian Jia, Xiaohan Shi, Yidong Xie, Xiaochuan Xie, Yan Wang, Shangwei Li

ABSTRACT

BACKGROUND: To reduce young female fertility loss, the in-vitro culture of cryopreserved ovarian cortical tissues (OCTs) is considered an effective approach without delaying treatment and undergoing stimulation medicine. However, ischemic damage and follicular loss during the in-vitro culture of OCTs are major technical challenges. Human umbilical cord stem cells (HUMSCs) and their conditioned medium (HUMSC-CM) have been considered to be potential resources for regeneration medicine because they secrete cytokines and enhance cell survival and function. The aim of this study was to determine whether HUMSC-CM improves the development of frozen-thawed in-vitro cultured ovarian tissues compared with a serum-free culture medium (SF-CM). METHODS: The thawed OCTs (n = 68) were cultivated in HUMSC-CM and SF-CM in vitro for 8 days, and the ovarian tissues were processed and analyzed by a classical histological evaluation. The microvessel density (MVD) and apotosis detection during in-vitro culture of OCTs were also performed. RESULTS: A significant difference in the rate of morphologically normal primordial follicles in the HUMSC-CM group was observed compared to that in the SF-CM group (group C) from days 2 to 4 (day 2: group B 58.0 ± 2.45% vs group C 32.0 ± 5.83%, p = 0.002; day 3: group B 55.5 ± 4.20% vs group C 21.0 ± 9.80%, p = 0.048; day 4: group B 52.0 ± 4.08% vs group C 21.5 ± 8.19%, p = 0.019). The microvessel density (MVD) detection showed a time-dependent increase and peaked on day 4. There was a significant difference between groups B (49.33 ± 0.58) and C (24.33 ± 3.79) (p = 0.036). The percentage of apoptotic follicles in group B was lower than that in group C on day 1 (13.75 ± 2.50% vs 27.0 ± 10.10%, p = 0.003), day 5 (11.75 ± 1.50% vs 51.0 ± 10.5%, p = 0.019) and day 7 (15.0 ± 5.10% vs 46.5 ± 21.75%, p = 0.018). CONCLUSIONS: These data have provided the first experimental evidence of the effect of HUMSC-CM on frozen-thawed OCTs in vitro. The results showed that the HUMSC-CM group provided a better protecting effect on the in-vitro culture of the cryopreserved OCTs compared to the SF-CM group. More... »

PAGES

152

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13287-017-0604-4

DOI

http://dx.doi.org/10.1186/s13287-017-0604-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1086155944

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28646900


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adult", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cryopreservation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Media, Conditioned", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Media, Serum-Free", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Organ Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ovary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Umbilical Cord", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "West China Second University Hospital of Sichuan University", 
          "id": "https://www.grid.ac/institutes/grid.461863.e", 
          "name": [
            "Division of Reproductive Medical Center, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China", 
            "Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital of Sichuan University, Chengdu, Sichuan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jia", 
        "givenName": "Yingxian", 
        "id": "sg:person.011622774721.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011622774721.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "West China Second University Hospital of Sichuan University", 
          "id": "https://www.grid.ac/institutes/grid.461863.e", 
          "name": [
            "Division of Reproductive Medical Center, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China", 
            "Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital of Sichuan University, Chengdu, Sichuan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shi", 
        "givenName": "Xiaohan", 
        "id": "sg:person.01320136133.65", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320136133.65"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "West China Second University Hospital of Sichuan University", 
          "id": "https://www.grid.ac/institutes/grid.461863.e", 
          "name": [
            "Division of Reproductive Medical Center, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China", 
            "Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital of Sichuan University, Chengdu, Sichuan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xie", 
        "givenName": "Yidong", 
        "id": "sg:person.012100746123.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012100746123.86"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "West China Hospital of Sichuan University", 
          "id": "https://www.grid.ac/institutes/grid.412901.f", 
          "name": [
            "Department of Cardiology, West China Hospital, Sichuan University, Chengdu, Sichuan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xie", 
        "givenName": "Xiaochuan", 
        "id": "sg:person.01252022733.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01252022733.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "West China Second University Hospital of Sichuan University", 
          "id": "https://www.grid.ac/institutes/grid.461863.e", 
          "name": [
            "Division of Reproductive Medical Center, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China", 
            "Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital of Sichuan University, Chengdu, Sichuan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Yan", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "West China Second University Hospital of Sichuan University", 
          "id": "https://www.grid.ac/institutes/grid.461863.e", 
          "name": [
            "Division of Reproductive Medical Center, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China", 
            "Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital of Sichuan University, Chengdu, Sichuan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Shangwei", 
        "id": "sg:person.0612151730.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612151730.32"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1177/1043454214532025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001639308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/1043454214532025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001639308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jcyt.2014.11.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001927381"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.5582/bst.2016.01125", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003740286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10815-015-0628-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007683699", 
          "https://doi.org/10.1007/s10815-015-0628-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod60.6.1462", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010505616"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod60.6.1462", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010505616"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/jcmm.12571", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015081690"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrclinonc.2010.133", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016742348", 
          "https://doi.org/10.1038/nrclinonc.2010.133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrclinonc.2010.133", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016742348", 
          "https://doi.org/10.1038/nrclinonc.2010.133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/14653249.2012.658911", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017135765"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12265-014-9585-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017764105", 
          "https://doi.org/10.1007/s12265-014-9585-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/ta.0000000000000547", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018703916"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/ta.0000000000000547", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018703916"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humrep/der385", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024873704"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/14653240802035926", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027882790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00441-016-2405-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027901690", 
          "https://doi.org/10.1007/s00441-016-2405-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00441-016-2405-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027901690", 
          "https://doi.org/10.1007/s00441-016-2405-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humrep/den070", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028013610"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/10428194.2015.1065980", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028740755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humrep/den255", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030271625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.fertnstert.2008.05.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031094381"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humrep/14.6.1555", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031749221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s2213-8587(15)00039-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032719863"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.rbmo.2010.10.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032810637"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3727/096368915x687787", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032985390"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3727/096368915x687787", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032985390"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humrep/deq055", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038235168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humrep/deq055", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038235168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/jgh.12893", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041697450"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.taap.2011.11.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045639625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/annonc/mds514", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046711387"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1472-6483(10)60912-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053433415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/scd.2016.0244", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059311345"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humrep/dew144", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059648811"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/1933719115578922", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064079425"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/1933719115578922", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064079425"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/1574888x11308020005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069216141"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078845397", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-12", 
    "datePublishedReg": "2017-12-01", 
    "description": "BACKGROUND: To reduce young female fertility loss, the in-vitro culture of cryopreserved ovarian cortical tissues (OCTs) is considered an effective approach without delaying treatment and undergoing stimulation medicine. However, ischemic damage and follicular loss during the in-vitro culture of OCTs are major technical challenges. Human umbilical cord stem cells (HUMSCs) and their conditioned medium (HUMSC-CM) have been considered to be potential resources for regeneration medicine because they secrete cytokines and enhance cell survival and function. The aim of this study was to determine whether HUMSC-CM improves the development of frozen-thawed in-vitro cultured ovarian tissues compared with a serum-free culture medium (SF-CM).\nMETHODS: The thawed OCTs (n\u2009=\u200968) were cultivated in HUMSC-CM and SF-CM in vitro for 8\u00a0days, and the ovarian tissues were processed and analyzed by a classical histological evaluation. The microvessel density (MVD) and apotosis detection during in-vitro culture of OCTs were also performed.\nRESULTS: A significant difference in the rate of morphologically normal primordial follicles in the HUMSC-CM group was observed compared to that in the SF-CM group (group C) from days 2 to 4 (day 2: group B 58.0\u2009\u00b1\u20092.45% vs group C 32.0\u2009\u00b1\u20095.83%, p\u2009=\u20090.002; day 3: group B 55.5\u2009\u00b1\u20094.20% vs group C 21.0\u2009\u00b1\u20099.80%, p\u2009=\u20090.048; day 4: group B 52.0\u2009\u00b1\u20094.08% vs group C 21.5\u2009\u00b1\u20098.19%, p\u2009=\u20090.019). The microvessel density (MVD) detection showed a time-dependent increase and peaked on day 4. There was a significant difference between groups B (49.33\u2009\u00b1\u20090.58) and C (24.33\u2009\u00b1\u20093.79) (p\u2009=\u20090.036). The percentage of apoptotic follicles in group B was lower than that in group C on day 1 (13.75\u2009\u00b1\u20092.50% vs 27.0\u2009\u00b1\u200910.10%, p\u2009=\u20090.003), day 5 (11.75\u2009\u00b1\u20091.50% vs 51.0\u2009\u00b1\u200910.5%, p\u2009=\u20090.019) and day 7 (15.0\u2009\u00b1\u20095.10% vs 46.5\u2009\u00b1\u200921.75%, p\u2009=\u20090.018).\nCONCLUSIONS: These data have provided the first experimental evidence of the effect of HUMSC-CM on frozen-thawed OCTs in vitro. The results showed that the HUMSC-CM group provided a better protecting effect on the in-vitro culture of the cryopreserved OCTs compared to the SF-CM group.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s13287-017-0604-4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.7001041", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1043222", 
        "issn": [
          "1757-6512"
        ], 
        "name": "Stem Cell Research & Therapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "name": "Human umbilical cord stem cell conditioned medium versus serum-free culture medium in the treatment of cryopreserved human ovarian tissues in in-vitro culture: a randomized controlled trial", 
    "pagination": "152", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a92065fb2438e4b5cc24a9ccb418260a82e8b6f983dcdfb6f8aacdba50c2eab2"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28646900"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101527581"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s13287-017-0604-4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1086155944"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s13287-017-0604-4", 
      "https://app.dimensions.ai/details/publication/pub.1086155944"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:38", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000363_0000000363/records_70040_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs13287-017-0604-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13287-017-0604-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13287-017-0604-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13287-017-0604-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13287-017-0604-4'


 

This table displays all metadata directly associated to this object as RDF triples.

244 TRIPLES      21 PREDICATES      70 URIs      31 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s13287-017-0604-4 schema:about N11537fe855a54f538256e7810d8be04e
2 N1ab829f6603841ed9908aa930a63cebe
3 N2f4c4f5ee05f4cc8a26b11ab662c41ce
4 N3252bf5021c9402eb00600cf60f30a87
5 N4661d8262aa040a480ec44fd98c2823d
6 N5eb6d3d52108486da8016c744bcd6e6d
7 N7a43434a80c94755bb1033afc35c0b41
8 N9864bda45f73490cb9f3852197c1254d
9 Na95c202acddd44b79e30dc218097033e
10 Nabbed293a968419a84b9fdfc4e87ab9f
11 anzsrc-for:11
12 anzsrc-for:1103
13 schema:author N84700b2469a24428b35282f99ee331ed
14 schema:citation sg:pub.10.1007/s00441-016-2405-y
15 sg:pub.10.1007/s10815-015-0628-6
16 sg:pub.10.1007/s12265-014-9585-1
17 sg:pub.10.1038/nrclinonc.2010.133
18 https://app.dimensions.ai/details/publication/pub.1078845397
19 https://doi.org/10.1016/j.fertnstert.2008.05.012
20 https://doi.org/10.1016/j.jcyt.2014.11.009
21 https://doi.org/10.1016/j.rbmo.2010.10.015
22 https://doi.org/10.1016/j.taap.2011.11.008
23 https://doi.org/10.1016/s1472-6483(10)60912-1
24 https://doi.org/10.1016/s2213-8587(15)00039-x
25 https://doi.org/10.1080/14653240802035926
26 https://doi.org/10.1089/scd.2016.0244
27 https://doi.org/10.1093/annonc/mds514
28 https://doi.org/10.1093/humrep/14.6.1555
29 https://doi.org/10.1093/humrep/den070
30 https://doi.org/10.1093/humrep/den255
31 https://doi.org/10.1093/humrep/deq055
32 https://doi.org/10.1093/humrep/der385
33 https://doi.org/10.1093/humrep/dew144
34 https://doi.org/10.1095/biolreprod60.6.1462
35 https://doi.org/10.1097/ta.0000000000000547
36 https://doi.org/10.1111/jcmm.12571
37 https://doi.org/10.1111/jgh.12893
38 https://doi.org/10.1177/1043454214532025
39 https://doi.org/10.1177/1933719115578922
40 https://doi.org/10.2174/1574888x11308020005
41 https://doi.org/10.3109/10428194.2015.1065980
42 https://doi.org/10.3109/14653249.2012.658911
43 https://doi.org/10.3727/096368915x687787
44 https://doi.org/10.5582/bst.2016.01125
45 schema:datePublished 2017-12
46 schema:datePublishedReg 2017-12-01
47 schema:description BACKGROUND: To reduce young female fertility loss, the in-vitro culture of cryopreserved ovarian cortical tissues (OCTs) is considered an effective approach without delaying treatment and undergoing stimulation medicine. However, ischemic damage and follicular loss during the in-vitro culture of OCTs are major technical challenges. Human umbilical cord stem cells (HUMSCs) and their conditioned medium (HUMSC-CM) have been considered to be potential resources for regeneration medicine because they secrete cytokines and enhance cell survival and function. The aim of this study was to determine whether HUMSC-CM improves the development of frozen-thawed in-vitro cultured ovarian tissues compared with a serum-free culture medium (SF-CM). METHODS: The thawed OCTs (n = 68) were cultivated in HUMSC-CM and SF-CM in vitro for 8 days, and the ovarian tissues were processed and analyzed by a classical histological evaluation. The microvessel density (MVD) and apotosis detection during in-vitro culture of OCTs were also performed. RESULTS: A significant difference in the rate of morphologically normal primordial follicles in the HUMSC-CM group was observed compared to that in the SF-CM group (group C) from days 2 to 4 (day 2: group B 58.0 ± 2.45% vs group C 32.0 ± 5.83%, p = 0.002; day 3: group B 55.5 ± 4.20% vs group C 21.0 ± 9.80%, p = 0.048; day 4: group B 52.0 ± 4.08% vs group C 21.5 ± 8.19%, p = 0.019). The microvessel density (MVD) detection showed a time-dependent increase and peaked on day 4. There was a significant difference between groups B (49.33 ± 0.58) and C (24.33 ± 3.79) (p = 0.036). The percentage of apoptotic follicles in group B was lower than that in group C on day 1 (13.75 ± 2.50% vs 27.0 ± 10.10%, p = 0.003), day 5 (11.75 ± 1.50% vs 51.0 ± 10.5%, p = 0.019) and day 7 (15.0 ± 5.10% vs 46.5 ± 21.75%, p = 0.018). CONCLUSIONS: These data have provided the first experimental evidence of the effect of HUMSC-CM on frozen-thawed OCTs in vitro. The results showed that the HUMSC-CM group provided a better protecting effect on the in-vitro culture of the cryopreserved OCTs compared to the SF-CM group.
48 schema:genre research_article
49 schema:inLanguage en
50 schema:isAccessibleForFree true
51 schema:isPartOf N43e83d4094b743d9a1bb6cf28de574e9
52 Ndd9bb3d0ae04457b935aa53a9438d314
53 sg:journal.1043222
54 schema:name Human umbilical cord stem cell conditioned medium versus serum-free culture medium in the treatment of cryopreserved human ovarian tissues in in-vitro culture: a randomized controlled trial
55 schema:pagination 152
56 schema:productId N18d41e5db2324348bdd7f07b46c8b31a
57 N5ee38b9f7aa6418386918126da7ac235
58 N9d121659c32f4ec38c2240c33e846280
59 Ndcffafea1617456cb73a2b9bf518462b
60 Ned872b448d86412bb84f6c016915f4b4
61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1086155944
62 https://doi.org/10.1186/s13287-017-0604-4
63 schema:sdDatePublished 2019-04-11T12:38
64 schema:sdLicense https://scigraph.springernature.com/explorer/license/
65 schema:sdPublisher N35735fea45da408b98b2bf35beb551a1
66 schema:url https://link.springer.com/10.1186%2Fs13287-017-0604-4
67 sgo:license sg:explorer/license/
68 sgo:sdDataset articles
69 rdf:type schema:ScholarlyArticle
70 N11537fe855a54f538256e7810d8be04e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Culture Media, Conditioned
72 rdf:type schema:DefinedTerm
73 N162bdf979c714c5894b58e8519978738 rdf:first sg:person.01252022733.23
74 rdf:rest N63203541935044a8a971803ea539fd14
75 N18d41e5db2324348bdd7f07b46c8b31a schema:name doi
76 schema:value 10.1186/s13287-017-0604-4
77 rdf:type schema:PropertyValue
78 N1ab829f6603841ed9908aa930a63cebe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Cryopreservation
80 rdf:type schema:DefinedTerm
81 N2f4c4f5ee05f4cc8a26b11ab662c41ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Ovary
83 rdf:type schema:DefinedTerm
84 N3252bf5021c9402eb00600cf60f30a87 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Organ Culture Techniques
86 rdf:type schema:DefinedTerm
87 N35735fea45da408b98b2bf35beb551a1 schema:name Springer Nature - SN SciGraph project
88 rdf:type schema:Organization
89 N43e83d4094b743d9a1bb6cf28de574e9 schema:issueNumber 1
90 rdf:type schema:PublicationIssue
91 N4661d8262aa040a480ec44fd98c2823d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Female
93 rdf:type schema:DefinedTerm
94 N5eb6d3d52108486da8016c744bcd6e6d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Stem Cells
96 rdf:type schema:DefinedTerm
97 N5ee38b9f7aa6418386918126da7ac235 schema:name nlm_unique_id
98 schema:value 101527581
99 rdf:type schema:PropertyValue
100 N63203541935044a8a971803ea539fd14 rdf:first N6dda8e353bcc403aa2c2aace80d06236
101 rdf:rest N66deb3052ec34a5b9585ec1602e64ada
102 N66deb3052ec34a5b9585ec1602e64ada rdf:first sg:person.0612151730.32
103 rdf:rest rdf:nil
104 N6dda8e353bcc403aa2c2aace80d06236 schema:affiliation https://www.grid.ac/institutes/grid.461863.e
105 schema:familyName Wang
106 schema:givenName Yan
107 rdf:type schema:Person
108 N7370c9cd0d6d4a2bb27b57e40f5bcb7b rdf:first sg:person.012100746123.86
109 rdf:rest N162bdf979c714c5894b58e8519978738
110 N7a43434a80c94755bb1033afc35c0b41 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Adult
112 rdf:type schema:DefinedTerm
113 N84700b2469a24428b35282f99ee331ed rdf:first sg:person.011622774721.52
114 rdf:rest Ne2bedf00de224423b0d026adca274451
115 N9864bda45f73490cb9f3852197c1254d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Umbilical Cord
117 rdf:type schema:DefinedTerm
118 N9d121659c32f4ec38c2240c33e846280 schema:name pubmed_id
119 schema:value 28646900
120 rdf:type schema:PropertyValue
121 Na95c202acddd44b79e30dc218097033e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Culture Media, Serum-Free
123 rdf:type schema:DefinedTerm
124 Nabbed293a968419a84b9fdfc4e87ab9f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Humans
126 rdf:type schema:DefinedTerm
127 Ndcffafea1617456cb73a2b9bf518462b schema:name readcube_id
128 schema:value a92065fb2438e4b5cc24a9ccb418260a82e8b6f983dcdfb6f8aacdba50c2eab2
129 rdf:type schema:PropertyValue
130 Ndd9bb3d0ae04457b935aa53a9438d314 schema:volumeNumber 8
131 rdf:type schema:PublicationVolume
132 Ne2bedf00de224423b0d026adca274451 rdf:first sg:person.01320136133.65
133 rdf:rest N7370c9cd0d6d4a2bb27b57e40f5bcb7b
134 Ned872b448d86412bb84f6c016915f4b4 schema:name dimensions_id
135 schema:value pub.1086155944
136 rdf:type schema:PropertyValue
137 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
138 schema:name Medical and Health Sciences
139 rdf:type schema:DefinedTerm
140 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
141 schema:name Clinical Sciences
142 rdf:type schema:DefinedTerm
143 sg:grant.7001041 http://pending.schema.org/fundedItem sg:pub.10.1186/s13287-017-0604-4
144 rdf:type schema:MonetaryGrant
145 sg:journal.1043222 schema:issn 1757-6512
146 schema:name Stem Cell Research & Therapy
147 rdf:type schema:Periodical
148 sg:person.011622774721.52 schema:affiliation https://www.grid.ac/institutes/grid.461863.e
149 schema:familyName Jia
150 schema:givenName Yingxian
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011622774721.52
152 rdf:type schema:Person
153 sg:person.012100746123.86 schema:affiliation https://www.grid.ac/institutes/grid.461863.e
154 schema:familyName Xie
155 schema:givenName Yidong
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012100746123.86
157 rdf:type schema:Person
158 sg:person.01252022733.23 schema:affiliation https://www.grid.ac/institutes/grid.412901.f
159 schema:familyName Xie
160 schema:givenName Xiaochuan
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01252022733.23
162 rdf:type schema:Person
163 sg:person.01320136133.65 schema:affiliation https://www.grid.ac/institutes/grid.461863.e
164 schema:familyName Shi
165 schema:givenName Xiaohan
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320136133.65
167 rdf:type schema:Person
168 sg:person.0612151730.32 schema:affiliation https://www.grid.ac/institutes/grid.461863.e
169 schema:familyName Li
170 schema:givenName Shangwei
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612151730.32
172 rdf:type schema:Person
173 sg:pub.10.1007/s00441-016-2405-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1027901690
174 https://doi.org/10.1007/s00441-016-2405-y
175 rdf:type schema:CreativeWork
176 sg:pub.10.1007/s10815-015-0628-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007683699
177 https://doi.org/10.1007/s10815-015-0628-6
178 rdf:type schema:CreativeWork
179 sg:pub.10.1007/s12265-014-9585-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017764105
180 https://doi.org/10.1007/s12265-014-9585-1
181 rdf:type schema:CreativeWork
182 sg:pub.10.1038/nrclinonc.2010.133 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016742348
183 https://doi.org/10.1038/nrclinonc.2010.133
184 rdf:type schema:CreativeWork
185 https://app.dimensions.ai/details/publication/pub.1078845397 schema:CreativeWork
186 https://doi.org/10.1016/j.fertnstert.2008.05.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031094381
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1016/j.jcyt.2014.11.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001927381
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/j.rbmo.2010.10.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032810637
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/j.taap.2011.11.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045639625
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/s1472-6483(10)60912-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053433415
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/s2213-8587(15)00039-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1032719863
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1080/14653240802035926 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027882790
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1089/scd.2016.0244 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059311345
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1093/annonc/mds514 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046711387
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1093/humrep/14.6.1555 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031749221
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1093/humrep/den070 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028013610
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1093/humrep/den255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030271625
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1093/humrep/deq055 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038235168
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1093/humrep/der385 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024873704
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1093/humrep/dew144 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059648811
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1095/biolreprod60.6.1462 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010505616
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1097/ta.0000000000000547 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018703916
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1111/jcmm.12571 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015081690
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1111/jgh.12893 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041697450
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1177/1043454214532025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001639308
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1177/1933719115578922 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064079425
227 rdf:type schema:CreativeWork
228 https://doi.org/10.2174/1574888x11308020005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069216141
229 rdf:type schema:CreativeWork
230 https://doi.org/10.3109/10428194.2015.1065980 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028740755
231 rdf:type schema:CreativeWork
232 https://doi.org/10.3109/14653249.2012.658911 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017135765
233 rdf:type schema:CreativeWork
234 https://doi.org/10.3727/096368915x687787 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032985390
235 rdf:type schema:CreativeWork
236 https://doi.org/10.5582/bst.2016.01125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003740286
237 rdf:type schema:CreativeWork
238 https://www.grid.ac/institutes/grid.412901.f schema:alternateName West China Hospital of Sichuan University
239 schema:name Department of Cardiology, West China Hospital, Sichuan University, Chengdu, Sichuan, China
240 rdf:type schema:Organization
241 https://www.grid.ac/institutes/grid.461863.e schema:alternateName West China Second University Hospital of Sichuan University
242 schema:name Division of Reproductive Medical Center, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China
243 Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital of Sichuan University, Chengdu, Sichuan, China
244 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...