An effective ex-vivo approach for inducing endothelial progenitor cells from umbilical cord blood CD34+ cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Meng Qin, Xin Guan, Huihui Wang, Yu Zhang, Bin Shen, Qingyu Zhang, Wei Dai, Yupo Ma, Yongping Jiang

ABSTRACT

BACKGROUND: Transplantation of endothelial progenitor cells (EPCs)/endothelial cells (ECs) has been used for the treatment of ischemic diseases and hemophilia A, due to their great capacity for producing factor VIII and for repairing vascular damage. We established an effective approach to stimulate the expansion and differentiation of EPCs for potential therapeutic applications. METHODS: CD34+ cells isolated from human cord blood were cultured in a two-step system for 21 days. The generated adherent cells were characterized via flow cytometry and immunofluorescent staining. Moreover, single-cell clonogenic and tube-forming assays were carried out to evaluate their potential to proliferate and form vessel networks. Furthermore, these cells were transplanted into a mouse model of hepatic sinusoidal endothelium injury by hepatic portal vein injection to investigate their in-vivo behavior. RESULTS: The two-step culture protocol promoted the expansion and differentiation of human cord blood CD34+ cells efficiently, resulting in a large number of adherent cells within 3 weeks. The generated adherent cells were identified as EPCs/ECs based on the expression of CD31, CD144, vWF, and FVIII, and cell numbers showed a 1400-fold increase compared with the initial number. Moreover, these EPCs/ECs were capable of proliferating and establishing colonies as individual cells, and forming tube-like structures. More significantly, tissue examination of mice after transplantation revealed that the injected EPCs/ECs migrated and integrated into the liver, reconstituting the sinusoidal endothelial compartment. CONCLUSIONS: We developed an approach for the generation of cord blood-derived EPCs/ECs on a large scale, characterized them phenotypically, and demonstrated their in-vivo functional capacity. Our approach provides an excellent source of healthy EPCs/ECs for use in cell therapy in a clinical setting. More... »

PAGES

25

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13287-017-0482-9

DOI

http://dx.doi.org/10.1186/s13287-017-0482-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1083720092

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28173870


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, CD", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cadherins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Adhesion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Movement", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Proliferation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chemical and Drug Induced Liver Injury", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Media", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endothelial Progenitor Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Factor VIII", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fetal Blood", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Liver", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred NOD", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, SCID", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Monocrotaline", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Platelet Endothelial Cell Adhesion Molecule-1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Primary Cell Culture", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transplantation, Heterologous", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "von Willebrand Factor", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China", 
            "Biopharmagen Corp., Suzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Qin", 
        "givenName": "Meng", 
        "id": "sg:person.012072654527.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012072654527.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Guan", 
        "givenName": "Xin", 
        "id": "sg:person.014021677077.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014021677077.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China", 
            "Biopharmagen Corp., Suzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Huihui", 
        "id": "sg:person.013465615527.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013465615527.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Yu", 
        "id": "sg:person.015414640077.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015414640077.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shen", 
        "givenName": "Bin", 
        "id": "sg:person.07507147277.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07507147277.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University at Albany, State University of New York", 
          "id": "https://www.grid.ac/institutes/grid.265850.c", 
          "name": [
            "Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China", 
            "School of Public Health, University at Albany, Albany, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Qingyu", 
        "id": "sg:person.015656137127.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015656137127.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "New York University Langone Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.240324.3", 
          "name": [
            "Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China", 
            "Department of Environmental Medicine, New York University Langone Medical Center, Tuxedo, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dai", 
        "givenName": "Wei", 
        "id": "sg:person.01110505215.87", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110505215.87"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Stony Brook University", 
          "id": "https://www.grid.ac/institutes/grid.36425.36", 
          "name": [
            "Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China", 
            "Department of Pathology, BST-9C, The State University of New York at Stony Brook, Stony Brook, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ma", 
        "givenName": "Yupo", 
        "id": "sg:person.0714543217.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714543217.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China", 
            "Biopharmagen Corp., Suzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jiang", 
        "givenName": "Yongping", 
        "id": "sg:person.01211174562.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211174562.47"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1161/01.atv.0000100403.78731.9f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001887533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.atv.0000100403.78731.9f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001887533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2006-12-062471", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003836824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11596-006-0408-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005724413", 
          "https://doi.org/10.1007/s11596-006-0408-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0042-6989(01)00237-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009075507"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10016-006-9047-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009525033", 
          "https://doi.org/10.1007/s10016-006-9047-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3727/096368912x657594", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010835317"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3727/096368912x657594", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010835317"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci32748", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017461161"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2141.2008.07519.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020719847"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2004-04-1396", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027487069"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/10428199309087003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027515426"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2013/190486", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028730127"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0023210", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029033431"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.atv.0000073832.49290.b5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036331493"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.atv.0000073832.49290.b5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036331493"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0812090106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036690415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.res.0000137877.89448.78", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039040939"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.res.0000137877.89448.78", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039040939"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci8296", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041932678"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0609.2005.00579.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043310088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0609.2005.00579.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043310088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1538-7836.2005.01508.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043416355"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1538-7836.2005.01508.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043416355"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.10-162040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046262425"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13287-016-0413-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046831389", 
          "https://doi.org/10.1186/s13287-016-0413-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13287-016-0413-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046831389", 
          "https://doi.org/10.1186/s13287-016-0413-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymthe.2005.11.021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050299059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12185-012-1015-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051844585", 
          "https://doi.org/10.1007/s12185-012-1015-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/10245330500093468", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058357976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.275.5302.964", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062555783"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3791/1524", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071422298"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.5966/sctm.2016-0224", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1073577816"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078649505", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078657795", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078774201", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-12", 
    "datePublishedReg": "2017-12-01", 
    "description": "BACKGROUND: Transplantation of endothelial progenitor cells (EPCs)/endothelial cells (ECs) has been used for the treatment of ischemic diseases and hemophilia A, due to their great capacity for producing factor VIII and for repairing vascular damage. We established an effective approach to stimulate the expansion and differentiation of EPCs for potential therapeutic applications.\nMETHODS: CD34+ cells isolated from human cord blood were cultured in a two-step system for 21\u00a0days. The generated adherent cells were characterized via flow cytometry and immunofluorescent staining. Moreover, single-cell clonogenic and tube-forming assays were carried out to evaluate their potential to proliferate and form vessel networks. Furthermore, these cells were transplanted into a mouse model of hepatic sinusoidal endothelium injury by hepatic portal vein injection to investigate their in-vivo behavior.\nRESULTS: The two-step culture protocol promoted the expansion and differentiation of human cord blood CD34+ cells efficiently, resulting in a large number of adherent cells within 3\u00a0weeks. The generated adherent cells were identified as EPCs/ECs based on the expression of CD31, CD144, vWF, and FVIII, and cell numbers showed a 1400-fold increase compared with the initial number. Moreover, these EPCs/ECs were capable of proliferating and establishing colonies as individual cells, and forming tube-like structures. More significantly, tissue examination of mice after transplantation revealed that the injected EPCs/ECs migrated and integrated into the liver, reconstituting the sinusoidal endothelial compartment.\nCONCLUSIONS: We developed an approach for the generation of cord blood-derived EPCs/ECs on a large scale, characterized them phenotypically, and demonstrated their in-vivo functional capacity. Our approach provides an excellent source of healthy EPCs/ECs for use in cell therapy in a clinical setting.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s13287-017-0482-9", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1043222", 
        "issn": [
          "1757-6512"
        ], 
        "name": "Stem Cell Research & Therapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "name": "An effective ex-vivo approach for inducing endothelial progenitor cells from umbilical cord blood CD34+ cells", 
    "pagination": "25", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "3aa83abc5824e1d455dd55542e01ce5c2574484e0d7450cec21861070f133644"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28173870"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101527581"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s13287-017-0482-9"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1083720092"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s13287-017-0482-9", 
      "https://app.dimensions.ai/details/publication/pub.1083720092"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:03", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89824_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs13287-017-0482-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13287-017-0482-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13287-017-0482-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13287-017-0482-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13287-017-0482-9'


 

This table displays all metadata directly associated to this object as RDF triples.

336 TRIPLES      21 PREDICATES      83 URIs      46 LITERALS      34 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s13287-017-0482-9 schema:about N148a2d4eb3fa4ad3bce45b56cd28075c
2 N1881fa138d614fe39b5a977cfd8e3761
3 N28cf5dd06bce43939576a65b6de16213
4 N2a41d6474e524fd79a4f7b29aa0df12e
5 N3ebada7477c14a07a450c11567d1ef99
6 N49ac36a235e9437b9160711322602dd7
7 N49e0deab8b244868a75180a227efd148
8 N579d6218270e46999e841dbec5b90813
9 N59395450edb14a25aa9c681eb1f4cf65
10 N5ad83e5b4c484c10bf8064fd6e3afa8f
11 N613c0a0b26334b369f528ccf9b0b559e
12 N62d84f2d8066465c9d392f6f1e75ba7b
13 N87e1540db98e48739fea257cf9a9a87c
14 N8af74d8fdd8445308d7198c3923289b7
15 N935b246303cb45c8885a17f502c0185b
16 N9c0974f81afa4629b3b02a641a0350f9
17 Na10eb7c3693e43929f0acd20be17de92
18 Na5a5c4fa719c4e1d90696aa525ec0736
19 Ncea89b221c0841f08f25e93cd22f098e
20 Nd2238c58747842ba8b363f4a5178621e
21 Ndde511166dd34baeb7993ea169d13687
22 Ne008bb5bbbcb4244aba178eb3887893b
23 Ne1a0cb53e9c24a60afee5dc4ef4411a0
24 Nec7dc2fc6dc14c3ca9ce272a5fb90e52
25 Nf4884ba749b14ab4a70c42b4081672aa
26 anzsrc-for:11
27 anzsrc-for:1107
28 schema:author Ncb4bdf592e644078bdfbc7e482895946
29 schema:citation sg:pub.10.1007/s10016-006-9047-6
30 sg:pub.10.1007/s11596-006-0408-8
31 sg:pub.10.1007/s12185-012-1015-z
32 sg:pub.10.1186/s13287-016-0413-1
33 https://app.dimensions.ai/details/publication/pub.1078649505
34 https://app.dimensions.ai/details/publication/pub.1078657795
35 https://app.dimensions.ai/details/publication/pub.1078774201
36 https://doi.org/10.1016/j.ymthe.2005.11.021
37 https://doi.org/10.1016/s0042-6989(01)00237-1
38 https://doi.org/10.1073/pnas.0812090106
39 https://doi.org/10.1080/10245330500093468
40 https://doi.org/10.1096/fj.10-162040
41 https://doi.org/10.1111/j.1365-2141.2008.07519.x
42 https://doi.org/10.1111/j.1538-7836.2005.01508.x
43 https://doi.org/10.1111/j.1600-0609.2005.00579.x
44 https://doi.org/10.1126/science.275.5302.964
45 https://doi.org/10.1155/2013/190486
46 https://doi.org/10.1161/01.atv.0000073832.49290.b5
47 https://doi.org/10.1161/01.atv.0000100403.78731.9f
48 https://doi.org/10.1161/01.res.0000137877.89448.78
49 https://doi.org/10.1172/jci32748
50 https://doi.org/10.1172/jci8296
51 https://doi.org/10.1182/blood-2004-04-1396
52 https://doi.org/10.1182/blood-2006-12-062471
53 https://doi.org/10.1371/journal.pone.0023210
54 https://doi.org/10.3109/10428199309087003
55 https://doi.org/10.3727/096368912x657594
56 https://doi.org/10.3791/1524
57 https://doi.org/10.5966/sctm.2016-0224
58 schema:datePublished 2017-12
59 schema:datePublishedReg 2017-12-01
60 schema:description BACKGROUND: Transplantation of endothelial progenitor cells (EPCs)/endothelial cells (ECs) has been used for the treatment of ischemic diseases and hemophilia A, due to their great capacity for producing factor VIII and for repairing vascular damage. We established an effective approach to stimulate the expansion and differentiation of EPCs for potential therapeutic applications. METHODS: CD34+ cells isolated from human cord blood were cultured in a two-step system for 21 days. The generated adherent cells were characterized via flow cytometry and immunofluorescent staining. Moreover, single-cell clonogenic and tube-forming assays were carried out to evaluate their potential to proliferate and form vessel networks. Furthermore, these cells were transplanted into a mouse model of hepatic sinusoidal endothelium injury by hepatic portal vein injection to investigate their in-vivo behavior. RESULTS: The two-step culture protocol promoted the expansion and differentiation of human cord blood CD34+ cells efficiently, resulting in a large number of adherent cells within 3 weeks. The generated adherent cells were identified as EPCs/ECs based on the expression of CD31, CD144, vWF, and FVIII, and cell numbers showed a 1400-fold increase compared with the initial number. Moreover, these EPCs/ECs were capable of proliferating and establishing colonies as individual cells, and forming tube-like structures. More significantly, tissue examination of mice after transplantation revealed that the injected EPCs/ECs migrated and integrated into the liver, reconstituting the sinusoidal endothelial compartment. CONCLUSIONS: We developed an approach for the generation of cord blood-derived EPCs/ECs on a large scale, characterized them phenotypically, and demonstrated their in-vivo functional capacity. Our approach provides an excellent source of healthy EPCs/ECs for use in cell therapy in a clinical setting.
61 schema:genre research_article
62 schema:inLanguage en
63 schema:isAccessibleForFree true
64 schema:isPartOf N4d67e4029bf4460e9bb32f7765d13d71
65 Ne9545e1e045e411ca1e777245a6fa178
66 sg:journal.1043222
67 schema:name An effective ex-vivo approach for inducing endothelial progenitor cells from umbilical cord blood CD34+ cells
68 schema:pagination 25
69 schema:productId N4df2d88e1b984b6c84ea5dffcbc954cc
70 N5a76fb7d8fd34f93a89712e7eda7bb33
71 N722765a728ab4f3696333a1f09393fbf
72 N94327d1d7339443ea376ecedfbd03db3
73 Nf0fd93af88064ecb954f10f4600aa0d4
74 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083720092
75 https://doi.org/10.1186/s13287-017-0482-9
76 schema:sdDatePublished 2019-04-11T10:03
77 schema:sdLicense https://scigraph.springernature.com/explorer/license/
78 schema:sdPublisher Ndc1f5260f46645039d316cfd8876aa96
79 schema:url https://link.springer.com/10.1186%2Fs13287-017-0482-9
80 sgo:license sg:explorer/license/
81 sgo:sdDataset articles
82 rdf:type schema:ScholarlyArticle
83 N148a2d4eb3fa4ad3bce45b56cd28075c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Mice, Inbred NOD
85 rdf:type schema:DefinedTerm
86 N1881fa138d614fe39b5a977cfd8e3761 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Antigens, CD
88 rdf:type schema:DefinedTerm
89 N2017d01b102d4bc093e2d78a4799969b rdf:first sg:person.01110505215.87
90 rdf:rest Nbd8da245bd79460aab4a605368f75691
91 N28cf5dd06bce43939576a65b6de16213 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Cell Proliferation
93 rdf:type schema:DefinedTerm
94 N2a41d6474e524fd79a4f7b29aa0df12e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Cell Adhesion
96 rdf:type schema:DefinedTerm
97 N2eea38670954496185e8669435eb0bd8 rdf:first sg:person.013465615527.13
98 rdf:rest Nd721fb185dd64c08970d7e246691174e
99 N3ebada7477c14a07a450c11567d1ef99 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Biomarkers
101 rdf:type schema:DefinedTerm
102 N49ac36a235e9437b9160711322602dd7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Male
104 rdf:type schema:DefinedTerm
105 N49e0deab8b244868a75180a227efd148 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Gene Expression
107 rdf:type schema:DefinedTerm
108 N4d508bbcff2e40779f21d35df2e4dfbb schema:name Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China
109 rdf:type schema:Organization
110 N4d67e4029bf4460e9bb32f7765d13d71 schema:issueNumber 1
111 rdf:type schema:PublicationIssue
112 N4df2d88e1b984b6c84ea5dffcbc954cc schema:name doi
113 schema:value 10.1186/s13287-017-0482-9
114 rdf:type schema:PropertyValue
115 N50ba9f4b8a5f4b92a7d8be0e3590ee34 rdf:first sg:person.015656137127.48
116 rdf:rest N2017d01b102d4bc093e2d78a4799969b
117 N579d6218270e46999e841dbec5b90813 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Fetal Blood
119 rdf:type schema:DefinedTerm
120 N59395450edb14a25aa9c681eb1f4cf65 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Mice, SCID
122 rdf:type schema:DefinedTerm
123 N5a76fb7d8fd34f93a89712e7eda7bb33 schema:name nlm_unique_id
124 schema:value 101527581
125 rdf:type schema:PropertyValue
126 N5ad83e5b4c484c10bf8064fd6e3afa8f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Monocrotaline
128 rdf:type schema:DefinedTerm
129 N613c0a0b26334b369f528ccf9b0b559e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Culture Media
131 rdf:type schema:DefinedTerm
132 N62d84f2d8066465c9d392f6f1e75ba7b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Primary Cell Culture
134 rdf:type schema:DefinedTerm
135 N722765a728ab4f3696333a1f09393fbf schema:name readcube_id
136 schema:value 3aa83abc5824e1d455dd55542e01ce5c2574484e0d7450cec21861070f133644
137 rdf:type schema:PropertyValue
138 N87e1540db98e48739fea257cf9a9a87c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Cell Movement
140 rdf:type schema:DefinedTerm
141 N8af74d8fdd8445308d7198c3923289b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Platelet Endothelial Cell Adhesion Molecule-1
143 rdf:type schema:DefinedTerm
144 N935b246303cb45c8885a17f502c0185b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Factor VIII
146 rdf:type schema:DefinedTerm
147 N94327d1d7339443ea376ecedfbd03db3 schema:name pubmed_id
148 schema:value 28173870
149 rdf:type schema:PropertyValue
150 N98492e3ad1c34878b5e7e5afbb4bd5b3 schema:name Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China
151 Biopharmagen Corp., Suzhou, China
152 rdf:type schema:Organization
153 N9b4ed7663c2e4adab12aae0acd6ea7f4 schema:name Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China
154 Biopharmagen Corp., Suzhou, China
155 rdf:type schema:Organization
156 N9c0974f81afa4629b3b02a641a0350f9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Cell Differentiation
158 rdf:type schema:DefinedTerm
159 Na10eb7c3693e43929f0acd20be17de92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Transplantation, Heterologous
161 rdf:type schema:DefinedTerm
162 Na5a5c4fa719c4e1d90696aa525ec0736 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Liver
164 rdf:type schema:DefinedTerm
165 Nbd8da245bd79460aab4a605368f75691 rdf:first sg:person.0714543217.39
166 rdf:rest Nd7bd0fb6bc5446efb195dbd4f363b5e6
167 Ncb4bdf592e644078bdfbc7e482895946 rdf:first sg:person.012072654527.77
168 rdf:rest Nd5e5621cb5ae4451a6f5d51e10079d0e
169 Ncea89b221c0841f08f25e93cd22f098e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Animals
171 rdf:type schema:DefinedTerm
172 Nd2238c58747842ba8b363f4a5178621e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Endothelial Progenitor Cells
174 rdf:type schema:DefinedTerm
175 Nd5e5621cb5ae4451a6f5d51e10079d0e rdf:first sg:person.014021677077.39
176 rdf:rest N2eea38670954496185e8669435eb0bd8
177 Nd721fb185dd64c08970d7e246691174e rdf:first sg:person.015414640077.38
178 rdf:rest Ndfcb23f018014ff8861a0fef7d7b9404
179 Nd7bd0fb6bc5446efb195dbd4f363b5e6 rdf:first sg:person.01211174562.47
180 rdf:rest rdf:nil
181 Ndc1f5260f46645039d316cfd8876aa96 schema:name Springer Nature - SN SciGraph project
182 rdf:type schema:Organization
183 Ndde511166dd34baeb7993ea169d13687 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name von Willebrand Factor
185 rdf:type schema:DefinedTerm
186 Ndfcb23f018014ff8861a0fef7d7b9404 rdf:first sg:person.07507147277.39
187 rdf:rest N50ba9f4b8a5f4b92a7d8be0e3590ee34
188 Ne008bb5bbbcb4244aba178eb3887893b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Chemical and Drug Induced Liver Injury
190 rdf:type schema:DefinedTerm
191 Ne1a0cb53e9c24a60afee5dc4ef4411a0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Humans
193 rdf:type schema:DefinedTerm
194 Ne1d0fe63eac84f89980d2ca209b483bd schema:name Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China
195 rdf:type schema:Organization
196 Ne82b7fdd8e934157a7eb4e838073c741 schema:name Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China
197 Biopharmagen Corp., Suzhou, China
198 rdf:type schema:Organization
199 Ne9545e1e045e411ca1e777245a6fa178 schema:volumeNumber 8
200 rdf:type schema:PublicationVolume
201 Nec7dc2fc6dc14c3ca9ce272a5fb90e52 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
202 schema:name Mice
203 rdf:type schema:DefinedTerm
204 Nf0fd93af88064ecb954f10f4600aa0d4 schema:name dimensions_id
205 schema:value pub.1083720092
206 rdf:type schema:PropertyValue
207 Nf4884ba749b14ab4a70c42b4081672aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
208 schema:name Cadherins
209 rdf:type schema:DefinedTerm
210 Nfaba0c97a974412facf924b590725fcb schema:name Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China
211 rdf:type schema:Organization
212 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
213 schema:name Medical and Health Sciences
214 rdf:type schema:DefinedTerm
215 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
216 schema:name Immunology
217 rdf:type schema:DefinedTerm
218 sg:journal.1043222 schema:issn 1757-6512
219 schema:name Stem Cell Research & Therapy
220 rdf:type schema:Periodical
221 sg:person.01110505215.87 schema:affiliation https://www.grid.ac/institutes/grid.240324.3
222 schema:familyName Dai
223 schema:givenName Wei
224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110505215.87
225 rdf:type schema:Person
226 sg:person.012072654527.77 schema:affiliation N98492e3ad1c34878b5e7e5afbb4bd5b3
227 schema:familyName Qin
228 schema:givenName Meng
229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012072654527.77
230 rdf:type schema:Person
231 sg:person.01211174562.47 schema:affiliation Ne82b7fdd8e934157a7eb4e838073c741
232 schema:familyName Jiang
233 schema:givenName Yongping
234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211174562.47
235 rdf:type schema:Person
236 sg:person.013465615527.13 schema:affiliation N9b4ed7663c2e4adab12aae0acd6ea7f4
237 schema:familyName Wang
238 schema:givenName Huihui
239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013465615527.13
240 rdf:type schema:Person
241 sg:person.014021677077.39 schema:affiliation N4d508bbcff2e40779f21d35df2e4dfbb
242 schema:familyName Guan
243 schema:givenName Xin
244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014021677077.39
245 rdf:type schema:Person
246 sg:person.015414640077.38 schema:affiliation Ne1d0fe63eac84f89980d2ca209b483bd
247 schema:familyName Zhang
248 schema:givenName Yu
249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015414640077.38
250 rdf:type schema:Person
251 sg:person.015656137127.48 schema:affiliation https://www.grid.ac/institutes/grid.265850.c
252 schema:familyName Zhang
253 schema:givenName Qingyu
254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015656137127.48
255 rdf:type schema:Person
256 sg:person.0714543217.39 schema:affiliation https://www.grid.ac/institutes/grid.36425.36
257 schema:familyName Ma
258 schema:givenName Yupo
259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714543217.39
260 rdf:type schema:Person
261 sg:person.07507147277.39 schema:affiliation Nfaba0c97a974412facf924b590725fcb
262 schema:familyName Shen
263 schema:givenName Bin
264 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07507147277.39
265 rdf:type schema:Person
266 sg:pub.10.1007/s10016-006-9047-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009525033
267 https://doi.org/10.1007/s10016-006-9047-6
268 rdf:type schema:CreativeWork
269 sg:pub.10.1007/s11596-006-0408-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005724413
270 https://doi.org/10.1007/s11596-006-0408-8
271 rdf:type schema:CreativeWork
272 sg:pub.10.1007/s12185-012-1015-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1051844585
273 https://doi.org/10.1007/s12185-012-1015-z
274 rdf:type schema:CreativeWork
275 sg:pub.10.1186/s13287-016-0413-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046831389
276 https://doi.org/10.1186/s13287-016-0413-1
277 rdf:type schema:CreativeWork
278 https://app.dimensions.ai/details/publication/pub.1078649505 schema:CreativeWork
279 https://app.dimensions.ai/details/publication/pub.1078657795 schema:CreativeWork
280 https://app.dimensions.ai/details/publication/pub.1078774201 schema:CreativeWork
281 https://doi.org/10.1016/j.ymthe.2005.11.021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050299059
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1016/s0042-6989(01)00237-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009075507
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1073/pnas.0812090106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036690415
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1080/10245330500093468 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058357976
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1096/fj.10-162040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046262425
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1111/j.1365-2141.2008.07519.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020719847
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1111/j.1538-7836.2005.01508.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043416355
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1111/j.1600-0609.2005.00579.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043310088
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1126/science.275.5302.964 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062555783
298 rdf:type schema:CreativeWork
299 https://doi.org/10.1155/2013/190486 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028730127
300 rdf:type schema:CreativeWork
301 https://doi.org/10.1161/01.atv.0000073832.49290.b5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036331493
302 rdf:type schema:CreativeWork
303 https://doi.org/10.1161/01.atv.0000100403.78731.9f schema:sameAs https://app.dimensions.ai/details/publication/pub.1001887533
304 rdf:type schema:CreativeWork
305 https://doi.org/10.1161/01.res.0000137877.89448.78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039040939
306 rdf:type schema:CreativeWork
307 https://doi.org/10.1172/jci32748 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017461161
308 rdf:type schema:CreativeWork
309 https://doi.org/10.1172/jci8296 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041932678
310 rdf:type schema:CreativeWork
311 https://doi.org/10.1182/blood-2004-04-1396 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027487069
312 rdf:type schema:CreativeWork
313 https://doi.org/10.1182/blood-2006-12-062471 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003836824
314 rdf:type schema:CreativeWork
315 https://doi.org/10.1371/journal.pone.0023210 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029033431
316 rdf:type schema:CreativeWork
317 https://doi.org/10.3109/10428199309087003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027515426
318 rdf:type schema:CreativeWork
319 https://doi.org/10.3727/096368912x657594 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010835317
320 rdf:type schema:CreativeWork
321 https://doi.org/10.3791/1524 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071422298
322 rdf:type schema:CreativeWork
323 https://doi.org/10.5966/sctm.2016-0224 schema:sameAs https://app.dimensions.ai/details/publication/pub.1073577816
324 rdf:type schema:CreativeWork
325 https://www.grid.ac/institutes/grid.240324.3 schema:alternateName New York University Langone Medical Center
326 schema:name Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China
327 Department of Environmental Medicine, New York University Langone Medical Center, Tuxedo, NY, USA
328 rdf:type schema:Organization
329 https://www.grid.ac/institutes/grid.265850.c schema:alternateName University at Albany, State University of New York
330 schema:name Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China
331 School of Public Health, University at Albany, Albany, NY, USA
332 rdf:type schema:Organization
333 https://www.grid.ac/institutes/grid.36425.36 schema:alternateName Stony Brook University
334 schema:name Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China
335 Department of Pathology, BST-9C, The State University of New York at Stony Brook, Stony Brook, NY, USA
336 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...