Tracking of epigenetic changes during hematopoietic differentiation of induced pluripotent stem cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Olivia Cypris, Joana Frobel, Shivam Rai, Julia Franzen, Stephanie Sontag, Roman Goetzke, Marcelo A. Szymanski de Toledo, Martin Zenke, Wolfgang Wagner

ABSTRACT

BACKGROUND: Differentiation of induced pluripotent stem cells (iPSCs) toward hematopoietic progenitor cells (HPCs) raises high hopes for disease modeling, drug screening, and cellular therapy. Various differentiation protocols have been established to generate iPSC-derived HPCs (iHPCs) that resemble their primary counterparts in morphology and immunophenotype, whereas a systematic epigenetic comparison was yet elusive. RESULTS: In this study, we compared genome-wide DNA methylation (DNAm) patterns of iHPCs with various different hematopoietic subsets. After 20 days of in vitro differentiation, cells revealed typical hematopoietic morphology, CD45 expression, and colony-forming unit (CFU) potential. DNAm changes were particularly observed in genes that are associated with hematopoietic differentiation. On the other hand, the epigenetic profiles of iHPCs remained overall distinct from natural HPCs. Furthermore, we analyzed if additional co-culture for 2 weeks with syngenic primary mesenchymal stromal cells (MSCs) or iPSC-derived MSCs (iMSCs) further supports epigenetic maturation toward the hematopoietic lineage. Proliferation of iHPCs and maintenance of CFU potential was enhanced upon co-culture. However, DNAm profiles support the notion that additional culture expansion with stromal support did not increase epigenetic maturation of iHPCs toward natural HPCs. CONCLUSION: Differentiation of iPSCs toward the hematopoietic lineage remains epigenetically incomplete. These results substantiate the need to elaborate advanced differentiation regimen while DNAm profiles provide a suitable measure to track this process. More... »

PAGES

19

References to SciGraph publications

  • 2011-05. A more efficient method to generate integration-free human iPS cells in NATURE METHODS
  • 2010-08. Mesenchymal and haematopoietic stem cells form a unique bone marrow niche in NATURE
  • 2009-11. DNA methylation protects hematopoietic stem cell multipotency from myeloerythroid restriction in NATURE GENETICS
  • 2015-12. The idiopathic preterm delivery methylation profile in umbilical cord blood DNA in BMC GENOMICS
  • 2008-08. Genome-scale DNA methylation maps of pluripotent and differentiated cells in NATURE
  • 2013-08. Co-transplantation of third-party umbilical cord blood-derived MSCs promotes engraftment in children undergoing unrelated umbilical cord blood transplantation in BONE MARROW TRANSPLANTATION
  • 2015-08. Epigenetic Biomarker to Support Classification into Pluripotent and Non-Pluripotent Cells in SCIENTIFIC REPORTS
  • 2012-12. DNA methylation arrays as surrogate measures of cell mixture distribution in BMC BIOINFORMATICS
  • 2017-05. Haematopoietic stem and progenitor cells from human pluripotent stem cells in NATURE
  • 2014-02. The relationship between DNA methylation, genetic and expression inter-individual variation in untransformed human fibroblasts in GENOME BIOLOGY
  • 2016-12. iPSC-derived mesenchymal stromal cells are less supportive than primary MSCs for co-culture of hematopoietic progenitor cells in JOURNAL OF HEMATOLOGY & ONCOLOGY
  • 2014-02. Aging of blood can be tracked by DNA methylation changes at just three CpG sites in GENOME BIOLOGY
  • 2013-12. Hematopoietic Stem and Progenitor Cells Acquire Distinct DNA-Hypermethylation During in vitro Culture in SCIENTIFIC REPORTS
  • 2005-12. High-Throughput GoMiner, an 'industrial-strength' integrative gene ontology tool for interpretation of multiple-microarray experiments, with application to studies of Common Variable Immune Deficiency (CVID) in BMC BIOINFORMATICS
  • 2012-11. Epigenomic analysis detects widespread gene-body DNA hypomethylation in chronic lymphocytic leukemia in NATURE GENETICS
  • 2017-01. A view of human haematopoietic development from the Petri dish in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2006-02. Superior ex vivo cord blood expansion following co-culture with bone marrow-derived mesenchymal stem cells in BONE MARROW TRANSPLANTATION
  • 2016-01. Generation of HLA-Universal iPSC-Derived Megakaryocytes and Platelets for Survival Under Refractoriness Conditions in MOLECULAR MEDICINE
  • Journal

    TITLE

    Clinical Epigenetics

    ISSUE

    1

    VOLUME

    11

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13148-019-0617-1

    DOI

    http://dx.doi.org/10.1186/s13148-019-0617-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1111907200

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30717806


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