Ontology type: schema:ScholarlyArticle Open Access: True
2018-12
AUTHORSMatthieu Le Gallo, Thibault de la Motte Rouge, Amanda Poissonnier, Vincent Lavoué, Patrick Tas, Jean Leveque, Florence Godey, Patrick Legembre
ABSTRACTOBJECTIVE: The use of novel methods to characterize living tumor cells relies on well-conceived biobanks. Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling. RESULTS: Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors. We examined living cells dissociated from TNBC and found that a classical freeze/thaw protocol leads to a marked reduction in the number of CD45-CD44LowCD24Low tumor cells. This, in turn, changed the percentage of tumor cells with certain CD44/CD24 expression patterns and changed the percentage of tumor-infiltrating immune cells. These cryopreservation-driven alterations in cellular phenotype make it impossible to compare fresh and frozen samples from the same patient directly. Moreover, the freeze/thaw process changed the transcriptomic signatures of triple-negative cancer stem cells in such a manner that hierarchical clustering no longer ranked them according to expected inter-individual differences. Overall, this study suggests that all analyses of living tumor cells should be conducted only using freshly dissociated tumors if we are to generate a robust scoring system for prognostic/predictive markers. More... »
PAGES401
http://scigraph.springernature.com/pub.10.1186/s13104-018-3504-5
DOIhttp://dx.doi.org/10.1186/s13104-018-3504-5
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1105008573
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/29925435
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Oncology and Carcinogenesis",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Biomarkers, Tumor",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "CD24 Antigen",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cryopreservation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Female",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Hyaluronan Receptors",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Leukocyte Common Antigens",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Specimen Handling",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Triple Negative Breast Neoplasms",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Rennes 1",
"id": "https://www.grid.ac/institutes/grid.410368.8",
"name": [
"COSS (Chemistry Oncogenesis Stress Signaling)-UMR 1242, Inserm, Univ Rennes, CLCC Eug\u00e8ne Marquis, Rue Bataille Flandres Dunkerque, 35042, Rennes, France",
"Equipe Labellis\u00e9e Ligue Contre Le Cancer, Rue Bataille Flandres Dunkerque, 35042, Rennes, France"
],
"type": "Organization"
},
"familyName": "Le Gallo",
"givenName": "Matthieu",
"id": "sg:person.0716452477.75",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716452477.75"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Rennes 1",
"id": "https://www.grid.ac/institutes/grid.410368.8",
"name": [
"COSS (Chemistry Oncogenesis Stress Signaling)-UMR 1242, Inserm, Univ Rennes, CLCC Eug\u00e8ne Marquis, Rue Bataille Flandres Dunkerque, 35042, Rennes, France",
"Equipe Labellis\u00e9e Ligue Contre Le Cancer, Rue Bataille Flandres Dunkerque, 35042, Rennes, France"
],
"type": "Organization"
},
"familyName": "de la Motte Rouge",
"givenName": "Thibault",
"id": "sg:person.01167112202.32",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167112202.32"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Oregon Health & Science University",
"id": "https://www.grid.ac/institutes/grid.5288.7",
"name": [
"COSS (Chemistry Oncogenesis Stress Signaling)-UMR 1242, Inserm, Univ Rennes, CLCC Eug\u00e8ne Marquis, Rue Bataille Flandres Dunkerque, 35042, Rennes, France",
"Equipe Labellis\u00e9e Ligue Contre Le Cancer, Rue Bataille Flandres Dunkerque, 35042, Rennes, France",
"Oregon Health and Science University, Portland, USA"
],
"type": "Organization"
},
"familyName": "Poissonnier",
"givenName": "Amanda",
"id": "sg:person.016165375651.39",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016165375651.39"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "l'H\u00f4pital Pontchaillou",
"id": "https://www.grid.ac/institutes/grid.414271.5",
"name": [
"COSS (Chemistry Oncogenesis Stress Signaling)-UMR 1242, Inserm, Univ Rennes, CLCC Eug\u00e8ne Marquis, Rue Bataille Flandres Dunkerque, 35042, Rennes, France",
"Equipe Labellis\u00e9e Ligue Contre Le Cancer, Rue Bataille Flandres Dunkerque, 35042, Rennes, France",
"CHU Pontchaillou, Rennes, France"
],
"type": "Organization"
},
"familyName": "Lavou\u00e9",
"givenName": "Vincent",
"id": "sg:person.01370764031.08",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01370764031.08"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "l'H\u00f4pital Pontchaillou",
"id": "https://www.grid.ac/institutes/grid.414271.5",
"name": [
"COSS (Chemistry Oncogenesis Stress Signaling)-UMR 1242, Inserm, Univ Rennes, CLCC Eug\u00e8ne Marquis, Rue Bataille Flandres Dunkerque, 35042, Rennes, France",
"Equipe Labellis\u00e9e Ligue Contre Le Cancer, Rue Bataille Flandres Dunkerque, 35042, Rennes, France",
"CHU Pontchaillou, Rennes, France"
],
"type": "Organization"
},
"familyName": "Tas",
"givenName": "Patrick",
"id": "sg:person.01335350344.07",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335350344.07"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "l'H\u00f4pital Pontchaillou",
"id": "https://www.grid.ac/institutes/grid.414271.5",
"name": [
"COSS (Chemistry Oncogenesis Stress Signaling)-UMR 1242, Inserm, Univ Rennes, CLCC Eug\u00e8ne Marquis, Rue Bataille Flandres Dunkerque, 35042, Rennes, France",
"Equipe Labellis\u00e9e Ligue Contre Le Cancer, Rue Bataille Flandres Dunkerque, 35042, Rennes, France",
"CHU Pontchaillou, Rennes, France"
],
"type": "Organization"
},
"familyName": "Leveque",
"givenName": "Jean",
"id": "sg:person.01206012023.14",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206012023.14"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Rennes 1",
"id": "https://www.grid.ac/institutes/grid.410368.8",
"name": [
"COSS (Chemistry Oncogenesis Stress Signaling)-UMR 1242, Inserm, Univ Rennes, CLCC Eug\u00e8ne Marquis, Rue Bataille Flandres Dunkerque, 35042, Rennes, France",
"Equipe Labellis\u00e9e Ligue Contre Le Cancer, Rue Bataille Flandres Dunkerque, 35042, Rennes, France"
],
"type": "Organization"
},
"familyName": "Godey",
"givenName": "Florence",
"id": "sg:person.01251751107.45",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01251751107.45"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Rennes 1",
"id": "https://www.grid.ac/institutes/grid.410368.8",
"name": [
"COSS (Chemistry Oncogenesis Stress Signaling)-UMR 1242, Inserm, Univ Rennes, CLCC Eug\u00e8ne Marquis, Rue Bataille Flandres Dunkerque, 35042, Rennes, France",
"Equipe Labellis\u00e9e Ligue Contre Le Cancer, Rue Bataille Flandres Dunkerque, 35042, Rennes, France"
],
"type": "Organization"
},
"familyName": "Legembre",
"givenName": "Patrick",
"id": "sg:person.0702316632.19",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702316632.19"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1016/j.theriogenology.2006.09.012",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007030281"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pmed.1002194",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010085602"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.stem.2014.02.002",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011388399"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.0530291100",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018724794"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.stem.2007.08.014",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045510569"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4161/cc.7.6.5591",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1072299890"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/cncr.31549",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1104131718"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/cncr.31551",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1104138692"
],
"type": "CreativeWork"
}
],
"datePublished": "2018-12",
"datePublishedReg": "2018-12-01",
"description": "OBJECTIVE: The use of novel methods to characterize living tumor cells relies on well-conceived biobanks. Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.\nRESULTS: Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors. We examined living cells dissociated from TNBC and found that a classical freeze/thaw protocol leads to a marked reduction in the number of CD45-CD44LowCD24Low tumor cells. This, in turn, changed the percentage of tumor cells with certain CD44/CD24 expression patterns and changed the percentage of tumor-infiltrating immune cells. These cryopreservation-driven alterations in cellular phenotype make it impossible to compare fresh and frozen samples from the same patient directly. Moreover, the freeze/thaw process changed the transcriptomic signatures of triple-negative cancer stem cells in such a manner that hierarchical clustering no longer ranked them according to expected inter-individual differences. Overall, this study suggests that all analyses of living tumor cells should be conducted only using freshly dissociated tumors if we are to generate a robust scoring system for prognostic/predictive markers.",
"genre": "research_article",
"id": "sg:pub.10.1186/s13104-018-3504-5",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1039457",
"issn": [
"1756-0500"
],
"name": "BMC Research Notes",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "11"
}
],
"name": "Tumor analysis: freeze\u2013thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells",
"pagination": "401",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"4842ef69359c4af2f903660d5ad1010aaef2cfdb34d6071e2bdd3ecf6e4a5a65"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"29925435"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"101462768"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1186/s13104-018-3504-5"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1105008573"
]
}
],
"sameAs": [
"https://doi.org/10.1186/s13104-018-3504-5",
"https://app.dimensions.ai/details/publication/pub.1105008573"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T22:42",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8690_00000571.jsonl",
"type": "ScholarlyArticle",
"url": "https://link.springer.com/10.1186%2Fs13104-018-3504-5"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13104-018-3504-5'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13104-018-3504-5'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13104-018-3504-5'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13104-018-3504-5'
This table displays all metadata directly associated to this object as RDF triples.
188 TRIPLES
21 PREDICATES
46 URIs
30 LITERALS
18 BLANK NODES