Effects of Escherichia coli Nissle 1917 and Ciprofloxacin on small intestinal epithelial cell mRNA expression in the neonatal piglet model ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-12

AUTHORS

Francine C. Paim, Stephanie N. Langel, David D. Fischer, Sukumar Kandasamy, Lulu Shao, Moyasar A. Alhamo, Huang-Chi Huang, Anand Kumar, Gireesh Rajashekara, Linda J. Saif, Anastasia N. Vlasova

ABSTRACT

We evaluated the effects of the probiotic Escherichia coli Nissle 1917 (EcN) and the antibiotic Ciprofloxacin (Cipro) on mRNA expression of intestinal epithelial cells (IEC) in gnotobiotic (Gn) piglets colonized with a defined commensal microflora (DMF) and inoculated with human rotavirus (HRV) that infects IECs. We analyzed mRNA levels of IEC genes for enteroendocrine cells [chromogranin A (CgA)], goblet cells [mucin 2 (MUC2)], transient amplifying progenitor cell [proliferating cell nuclear antigen (PCNA)], intestinal epithelial stem cell (SOX9) and enterocytes (villin). Cipro treatment enhanced HRV diarrhea and decreased the mRNA levels of MUC2 and villin but increased PCNA. These results suggest that Cipro alters the epithelial barrier, potentially decreasing the numbers of mature enterocytes (villin) and goblet cells secreting protective mucin (MUC2). These alterations may induce increased IEC proliferation (PCNA expression) to restore the integrity of the epithelial layer. Coincidental with decreased diarrhea severity in EcN treated groups, the expression of CgA and villin was increased, while SOX9 expression was decreased representing higher epithelial integrity indicative of inhibition of cellular proliferation. Thus, EcN protects the intestinal epithelium from damage by increasing the gene expression of enterocytes and enteroendocrine cells, maintaining the absorptive function and, consequently, decreasing the severity of diarrhea in HRV infection. More... »

PAGES

66

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13099-016-0148-7

DOI

http://dx.doi.org/10.1186/s13099-016-0148-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046258710

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27999620


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278 schema:name Food Animal Health Research Program (FAHRP), The Ohio Agricultural Research and Development Center, Veterinary Preventive Medicine Department, The Ohio State University, 1680 Madison Avenue, 44691, Wooster, OH, USA
279 rdf:type schema:Organization
 




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