The MTHFR promoter hypermethylation pattern associated with the A1298C polymorphism influences lipid parameters and glycemic control in diabetic patients View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Herlanny Santana Bezerra, Caroline Severo de Assis, Mayara Karla dos Santos Nunes, Isabella Wanderley de Queiroga Evangelista, João Modesto Filho, Cecília Neta Alves Pegado Gomes, Rayner Anderson Ferreira do Nascimento, Rafaella Cristhine Pordeus Luna, Maria José de Carvalho Costa, Naila Francis Paulo de Oliveira, Darlene Camati Persuhn

ABSTRACT

Background: Polymorphisms in the gene encoding methylenetetrahydrofolate reductase (MTHFR) have been investigated as risk factors for microvascular complications of diabetes; however, simultaneous analysis of these polymorphisms and the methylation pattern of the gene has never been conducted. The objective of the present study was to evaluate the simultaneous relationship between MTHFR methylation and MTHFR C6TT7 and A1298C polymorphisms with metabolic, inflammatory and oxidative stress parameters related to microvascular complications, diabetic retinopathy (DR) and diabetic nephropathy (DN) in diabetic patients. Methods: A total of 107 patients who were diagnosed in the previous 5 to 10 years were recruited and divided into groups with complications (DR and/or DN) or without complications. Methylation analysis of the gene promoter was conducted using the MSP technique, and analysis of the A1298C and C677T polymorphisms was conducted using the restriction fragment length polymorphism (RFLP) assay. Microalbuminuria was determined using urine samples, and other analytes of interest were determined in blood samples using commercial kits. The Mann-Whitney and Chi square statistical tests were used with significance considered at p < 0.05. Results: Subjects with a hypermethylated profile and the 1298AA genotype showed the highest levels of blood glucose (p = 0.03), total cholesterol (p = 0.0001) and LDL cholesterol (p = 0.0006). The same profile was associated with higher levels of HbA1c (p = 0.025), glycemia (p = 0.04) and total cholesterol (0.004) in the control group and total cholesterol (p = 0.005) and LDL cholesterol (p = 0.002) in the complications group. Serum creatinine was higher in subjects in the hypermethylated group with the genotype 677CC only in the control group (p = 0.0020). The methylated profile in presence of 677CC + 1298AA and the 677CT/TT +1298AA haplotypes showed higher levels of total cholesterol (p = 0.0024; 0.0031) and LDL cholesterol (p = 0.0060; 0.0125) than 1298AC/CC carriers. The fasting glycemia was higher in hypermethylated profile in the presence of 677CC/1298AA haplotype (p = 0.0077). Conclusion: The hypermethylated methylation profile associated with the 1298AA genotype appeared to be connected to higher values of glycemia, total cholesterol and LDL cholesterol. More... »

PAGES

4

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13098-019-0399-9

DOI

http://dx.doi.org/10.1186/s13098-019-0399-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111430408

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30675189


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Federal University of Para\u00edba", 
          "id": "https://www.grid.ac/institutes/grid.411216.1", 
          "name": [
            "Scientific Initiation Program, Federal University of Paraiba, Joao Pessoa, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Santana Bezerra", 
        "givenName": "Herlanny", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Para\u00edba", 
          "id": "https://www.grid.ac/institutes/grid.411216.1", 
          "name": [
            "Nutrition Sciences, Federal University of Paraiba, Joao Pessoa, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Severo de Assis", 
        "givenName": "Caroline", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Para\u00edba", 
          "id": "https://www.grid.ac/institutes/grid.411216.1", 
          "name": [
            "Development and Technological Innovation of Medicines, Federal University of Paraiba, Jo\u00e3o Pessoa, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "dos Santos Nunes", 
        "givenName": "Mayara Karla", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Para\u00edba", 
          "id": "https://www.grid.ac/institutes/grid.411216.1", 
          "name": [
            "Unidade da Vis\u00e3o, Hospital Universit\u00e1rio Lauro Wanderley, Federal University of Para\u00edba, Joao Pessoa, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wanderley de Queiroga Evangelista", 
        "givenName": "Isabella", 
        "id": "sg:person.013446272207.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013446272207.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Para\u00edba", 
          "id": "https://www.grid.ac/institutes/grid.411216.1", 
          "name": [
            "Department of Internal Medicine, Federal University of Paraiba, Joao Pessoa, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Modesto Filho", 
        "givenName": "Jo\u00e3o", 
        "id": "sg:person.014243652607.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014243652607.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Para\u00edba", 
          "id": "https://www.grid.ac/institutes/grid.411216.1", 
          "name": [
            "Nephrology Clinic, Lauro Wanderley University Hospital, Federal University of Paraiba, Joao Pessoa, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Alves Pegado Gomes", 
        "givenName": "Cec\u00edlia Neta", 
        "id": "sg:person.015041233207.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015041233207.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Faculty Mauricio of Nassau, Joao Pessoa, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ferreira do Nascimento", 
        "givenName": "Rayner Anderson", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Para\u00edba", 
          "id": "https://www.grid.ac/institutes/grid.411216.1", 
          "name": [
            "Post-Graduate Program in Nutrition Science, Federal University of Paraiba, Joao Pessoa, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pordeus Luna", 
        "givenName": "Rafaella Cristhine", 
        "id": "sg:person.01244272545.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244272545.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Para\u00edba", 
          "id": "https://www.grid.ac/institutes/grid.411216.1", 
          "name": [
            "Nutrition Science Department and Post-Graduate Program in Nutrition Science, Federal University of Paraiba, Joao Pessoa, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "de Carvalho Costa", 
        "givenName": "Maria Jos\u00e9", 
        "id": "sg:person.0754141234.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754141234.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Para\u00edba", 
          "id": "https://www.grid.ac/institutes/grid.411216.1", 
          "name": [
            "Department of Molecular Biology, Federal University of Paraiba, Joao Pessoa, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "de Oliveira", 
        "givenName": "Naila Francis Paulo", 
        "id": "sg:person.01047113467.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047113467.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Para\u00edba", 
          "id": "https://www.grid.ac/institutes/grid.411216.1", 
          "name": [
            "Department of Molecular Biology and Post-Graduate Program in Nutrition Science, Federal University of Paraiba, CEP 58051-900, Jo\u00e3o Pessoa, PB, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Camati Persuhn", 
        "givenName": "Darlene", 
        "id": "sg:person.01353724231.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01353724231.30"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/ng0595-111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007583916", 
          "https://doi.org/10.1038/ng0595-111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.clon.2011.03.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008202213"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2337/diacare.23.12.1816", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009413395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11010-011-0770-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011052190", 
          "https://doi.org/10.1007/s11010-011-0770-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m112.431627", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012055337"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/16.3.1215", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012912024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.6026/97320630009929", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017878121"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0005-2760(98)00086-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018463813"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.arcmed.2011.12.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026264002"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11239-009-0321-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026284332", 
          "https://doi.org/10.1007/s11239-009-0321-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11239-009-0321-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026284332", 
          "https://doi.org/10.1007/s11239-009-0321-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/nu7085303", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034621243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/1470320312444651", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037976186"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/1470320312444651", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037976186"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0378-1119(00)00392-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038166090"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/jmg.2005.032656", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038792307"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.metabol.2012.10.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041433059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11033-014-3729-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043027802", 
          "https://doi.org/10.1007/s11033-014-3729-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/1470320313516172", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044842297"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/1470320313516172", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044842297"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jdiacomp.2006.12.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044954413"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0026747", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046040082"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11033-013-2906-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049789268", 
          "https://doi.org/10.1007/s11033-013-2906-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11033-013-2906-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049789268", 
          "https://doi.org/10.1007/s11033-013-2906-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1139/o79-007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053084481"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/301825", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058609431"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jn/129.9.1656", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074511821"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075097685", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077466928", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079361788", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bsr20160611", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083738137"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13098-017-0284-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092270736", 
          "https://doi.org/10.1186/s13098-017-0284-3"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "Background: Polymorphisms in the gene encoding methylenetetrahydrofolate reductase (MTHFR) have been investigated as risk factors for microvascular complications of diabetes; however, simultaneous analysis of these polymorphisms and the methylation pattern of the gene has never been conducted. The objective of the present study was to evaluate the simultaneous relationship between MTHFR methylation and MTHFR C6TT7 and A1298C polymorphisms with metabolic, inflammatory and oxidative stress parameters related to microvascular complications, diabetic retinopathy (DR) and diabetic nephropathy (DN) in diabetic patients.\nMethods: A total of 107 patients who were diagnosed in the previous 5 to 10\u00a0years were recruited and divided into groups with complications (DR and/or DN) or without complications. Methylation analysis of the gene promoter was conducted using the MSP technique, and analysis of the A1298C and C677T polymorphisms was conducted using the restriction fragment length polymorphism (RFLP) assay. Microalbuminuria was determined using urine samples, and other analytes of interest were determined in blood samples using commercial kits. The Mann-Whitney and Chi square statistical tests were used with significance considered at p\u2009<\u20090.05.\nResults: Subjects with a hypermethylated profile and the 1298AA genotype showed the highest levels of blood glucose (p\u2009=\u20090.03), total cholesterol (p\u2009=\u20090.0001) and LDL cholesterol (p\u2009=\u20090.0006). The same profile was associated with higher levels of HbA1c (p\u2009=\u20090.025), glycemia (p\u2009=\u20090.04) and total cholesterol (0.004) in the control group and total cholesterol (p\u2009=\u20090.005) and LDL cholesterol (p\u2009=\u20090.002) in the complications group. Serum creatinine was higher in subjects in the hypermethylated group with the genotype 677CC only in the control group (p\u2009=\u20090.0020). The methylated profile in presence of 677CC\u2009+\u20091298AA and the 677CT/TT +1298AA haplotypes showed higher levels of total cholesterol (p\u2009=\u20090.0024; 0.0031) and LDL cholesterol (p\u2009=\u20090.0060; 0.0125) than 1298AC/CC carriers. The fasting glycemia was higher in hypermethylated profile in the presence of 677CC/1298AA haplotype (p\u2009=\u20090.0077).\nConclusion: The hypermethylated methylation profile associated with the 1298AA genotype appeared to be connected to higher values of glycemia, total cholesterol and LDL cholesterol.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s13098-019-0399-9", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1040785", 
        "issn": [
          "1758-5996"
        ], 
        "name": "Diabetology & Metabolic Syndrome", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "11"
      }
    ], 
    "name": "The MTHFR promoter hypermethylation pattern associated with the A1298C polymorphism influences lipid parameters and glycemic control in diabetic patients", 
    "pagination": "4", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "17813ed36f03a8438c9ac0aef004474a65dcabfee55f3685ebe2bfe9fe6a44a7"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30675189"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101488958"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s13098-019-0399-9"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1111430408"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s13098-019-0399-9", 
      "https://app.dimensions.ai/details/publication/pub.1111430408"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T08:57", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000325_0000000325/records_100809_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs13098-019-0399-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13098-019-0399-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13098-019-0399-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13098-019-0399-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13098-019-0399-9'


 

This table displays all metadata directly associated to this object as RDF triples.

232 TRIPLES      21 PREDICATES      57 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s13098-019-0399-9 schema:about anzsrc-for:11
2 anzsrc-for:1103
3 schema:author N17968a7afd794195a85111d97fc3a197
4 schema:citation sg:pub.10.1007/s11010-011-0770-0
5 sg:pub.10.1007/s11033-013-2906-7
6 sg:pub.10.1007/s11033-014-3729-x
7 sg:pub.10.1007/s11239-009-0321-7
8 sg:pub.10.1038/ng0595-111
9 sg:pub.10.1186/s13098-017-0284-3
10 https://app.dimensions.ai/details/publication/pub.1075097685
11 https://app.dimensions.ai/details/publication/pub.1077466928
12 https://app.dimensions.ai/details/publication/pub.1079361788
13 https://doi.org/10.1016/j.arcmed.2011.12.003
14 https://doi.org/10.1016/j.clon.2011.03.005
15 https://doi.org/10.1016/j.jdiacomp.2006.12.002
16 https://doi.org/10.1016/j.metabol.2012.10.004
17 https://doi.org/10.1016/s0005-2760(98)00086-1
18 https://doi.org/10.1016/s0378-1119(00)00392-9
19 https://doi.org/10.1042/bsr20160611
20 https://doi.org/10.1074/jbc.m112.431627
21 https://doi.org/10.1086/301825
22 https://doi.org/10.1093/jn/129.9.1656
23 https://doi.org/10.1093/nar/16.3.1215
24 https://doi.org/10.1136/jmg.2005.032656
25 https://doi.org/10.1139/o79-007
26 https://doi.org/10.1177/1470320312444651
27 https://doi.org/10.1177/1470320313516172
28 https://doi.org/10.1371/journal.pone.0026747
29 https://doi.org/10.2337/diacare.23.12.1816
30 https://doi.org/10.3390/nu7085303
31 https://doi.org/10.6026/97320630009929
32 schema:datePublished 2019-12
33 schema:datePublishedReg 2019-12-01
34 schema:description Background: Polymorphisms in the gene encoding methylenetetrahydrofolate reductase (MTHFR) have been investigated as risk factors for microvascular complications of diabetes; however, simultaneous analysis of these polymorphisms and the methylation pattern of the gene has never been conducted. The objective of the present study was to evaluate the simultaneous relationship between MTHFR methylation and MTHFR C6TT7 and A1298C polymorphisms with metabolic, inflammatory and oxidative stress parameters related to microvascular complications, diabetic retinopathy (DR) and diabetic nephropathy (DN) in diabetic patients. Methods: A total of 107 patients who were diagnosed in the previous 5 to 10 years were recruited and divided into groups with complications (DR and/or DN) or without complications. Methylation analysis of the gene promoter was conducted using the MSP technique, and analysis of the A1298C and C677T polymorphisms was conducted using the restriction fragment length polymorphism (RFLP) assay. Microalbuminuria was determined using urine samples, and other analytes of interest were determined in blood samples using commercial kits. The Mann-Whitney and Chi square statistical tests were used with significance considered at p < 0.05. Results: Subjects with a hypermethylated profile and the 1298AA genotype showed the highest levels of blood glucose (p = 0.03), total cholesterol (p = 0.0001) and LDL cholesterol (p = 0.0006). The same profile was associated with higher levels of HbA1c (p = 0.025), glycemia (p = 0.04) and total cholesterol (0.004) in the control group and total cholesterol (p = 0.005) and LDL cholesterol (p = 0.002) in the complications group. Serum creatinine was higher in subjects in the hypermethylated group with the genotype 677CC only in the control group (p = 0.0020). The methylated profile in presence of 677CC + 1298AA and the 677CT/TT +1298AA haplotypes showed higher levels of total cholesterol (p = 0.0024; 0.0031) and LDL cholesterol (p = 0.0060; 0.0125) than 1298AC/CC carriers. The fasting glycemia was higher in hypermethylated profile in the presence of 677CC/1298AA haplotype (p = 0.0077). Conclusion: The hypermethylated methylation profile associated with the 1298AA genotype appeared to be connected to higher values of glycemia, total cholesterol and LDL cholesterol.
35 schema:genre research_article
36 schema:inLanguage en
37 schema:isAccessibleForFree true
38 schema:isPartOf N956d45e25cf54c7aa9d9389b888eed59
39 Ncb6ee95ff6fb4bd2b2d6fd441f0beb6d
40 sg:journal.1040785
41 schema:name The MTHFR promoter hypermethylation pattern associated with the A1298C polymorphism influences lipid parameters and glycemic control in diabetic patients
42 schema:pagination 4
43 schema:productId N5807c8f8b34647ddab49acb5f448f565
44 Nba2fa936999f4ede97150614f38929d4
45 Nd4e7897124484516a506ebd34941b31b
46 Nd9fa43687a794ccda866220644691fb2
47 Neb86c80d8dad42ddb412de1546d3caad
48 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111430408
49 https://doi.org/10.1186/s13098-019-0399-9
50 schema:sdDatePublished 2019-04-11T08:57
51 schema:sdLicense https://scigraph.springernature.com/explorer/license/
52 schema:sdPublisher N201f9b905f624f27b02f996448d4531a
53 schema:url https://link.springer.com/10.1186%2Fs13098-019-0399-9
54 sgo:license sg:explorer/license/
55 sgo:sdDataset articles
56 rdf:type schema:ScholarlyArticle
57 N17968a7afd794195a85111d97fc3a197 rdf:first N4f158a97306742b3a6e41526a21b360a
58 rdf:rest Ncea4bda246174fb480d77e8ae2921a0b
59 N201f9b905f624f27b02f996448d4531a schema:name Springer Nature - SN SciGraph project
60 rdf:type schema:Organization
61 N24226d9fc26942eca063444bcaefe6b4 rdf:first sg:person.01244272545.21
62 rdf:rest Nb1c0408c583440d5b1041580114217ed
63 N250f191510134d45947d963353d160a8 rdf:first sg:person.01353724231.30
64 rdf:rest rdf:nil
65 N2ef2958b3e9445b9b930c793ae82b2ae rdf:first N93bd9e23b93642999f217c02974cdab2
66 rdf:rest N24226d9fc26942eca063444bcaefe6b4
67 N361dac4824bd4586be917e87b1f72657 schema:name Faculty Mauricio of Nassau, Joao Pessoa, Brazil
68 rdf:type schema:Organization
69 N48cb3cf3d8a24e7fb3ad4b0028386b0b rdf:first sg:person.014243652607.75
70 rdf:rest N6243c88722b64b128409e414be3e0079
71 N4f158a97306742b3a6e41526a21b360a schema:affiliation https://www.grid.ac/institutes/grid.411216.1
72 schema:familyName Santana Bezerra
73 schema:givenName Herlanny
74 rdf:type schema:Person
75 N5807c8f8b34647ddab49acb5f448f565 schema:name doi
76 schema:value 10.1186/s13098-019-0399-9
77 rdf:type schema:PropertyValue
78 N5a2431f1b2b0406f868daa9efc3c9f3c rdf:first sg:person.01047113467.35
79 rdf:rest N250f191510134d45947d963353d160a8
80 N6243c88722b64b128409e414be3e0079 rdf:first sg:person.015041233207.00
81 rdf:rest N2ef2958b3e9445b9b930c793ae82b2ae
82 N6c563a899b024f4f80ec3305e30cf8ac rdf:first sg:person.013446272207.03
83 rdf:rest N48cb3cf3d8a24e7fb3ad4b0028386b0b
84 N7af522a0d35e43439c11081e8f3856fc schema:affiliation https://www.grid.ac/institutes/grid.411216.1
85 schema:familyName Severo de Assis
86 schema:givenName Caroline
87 rdf:type schema:Person
88 N8baf85d46f5d41f29c1b0a5f9530350c schema:affiliation https://www.grid.ac/institutes/grid.411216.1
89 schema:familyName dos Santos Nunes
90 schema:givenName Mayara Karla
91 rdf:type schema:Person
92 N93bd9e23b93642999f217c02974cdab2 schema:affiliation N361dac4824bd4586be917e87b1f72657
93 schema:familyName Ferreira do Nascimento
94 schema:givenName Rayner Anderson
95 rdf:type schema:Person
96 N956d45e25cf54c7aa9d9389b888eed59 schema:issueNumber 1
97 rdf:type schema:PublicationIssue
98 Nb1c0408c583440d5b1041580114217ed rdf:first sg:person.0754141234.45
99 rdf:rest N5a2431f1b2b0406f868daa9efc3c9f3c
100 Nba2fa936999f4ede97150614f38929d4 schema:name pubmed_id
101 schema:value 30675189
102 rdf:type schema:PropertyValue
103 Nc015e9bd184647b3ab87dca74101bccd rdf:first N8baf85d46f5d41f29c1b0a5f9530350c
104 rdf:rest N6c563a899b024f4f80ec3305e30cf8ac
105 Ncb6ee95ff6fb4bd2b2d6fd441f0beb6d schema:volumeNumber 11
106 rdf:type schema:PublicationVolume
107 Ncea4bda246174fb480d77e8ae2921a0b rdf:first N7af522a0d35e43439c11081e8f3856fc
108 rdf:rest Nc015e9bd184647b3ab87dca74101bccd
109 Nd4e7897124484516a506ebd34941b31b schema:name nlm_unique_id
110 schema:value 101488958
111 rdf:type schema:PropertyValue
112 Nd9fa43687a794ccda866220644691fb2 schema:name dimensions_id
113 schema:value pub.1111430408
114 rdf:type schema:PropertyValue
115 Neb86c80d8dad42ddb412de1546d3caad schema:name readcube_id
116 schema:value 17813ed36f03a8438c9ac0aef004474a65dcabfee55f3685ebe2bfe9fe6a44a7
117 rdf:type schema:PropertyValue
118 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
119 schema:name Medical and Health Sciences
120 rdf:type schema:DefinedTerm
121 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
122 schema:name Clinical Sciences
123 rdf:type schema:DefinedTerm
124 sg:journal.1040785 schema:issn 1758-5996
125 schema:name Diabetology & Metabolic Syndrome
126 rdf:type schema:Periodical
127 sg:person.01047113467.35 schema:affiliation https://www.grid.ac/institutes/grid.411216.1
128 schema:familyName de Oliveira
129 schema:givenName Naila Francis Paulo
130 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047113467.35
131 rdf:type schema:Person
132 sg:person.01244272545.21 schema:affiliation https://www.grid.ac/institutes/grid.411216.1
133 schema:familyName Pordeus Luna
134 schema:givenName Rafaella Cristhine
135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244272545.21
136 rdf:type schema:Person
137 sg:person.013446272207.03 schema:affiliation https://www.grid.ac/institutes/grid.411216.1
138 schema:familyName Wanderley de Queiroga Evangelista
139 schema:givenName Isabella
140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013446272207.03
141 rdf:type schema:Person
142 sg:person.01353724231.30 schema:affiliation https://www.grid.ac/institutes/grid.411216.1
143 schema:familyName Camati Persuhn
144 schema:givenName Darlene
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01353724231.30
146 rdf:type schema:Person
147 sg:person.014243652607.75 schema:affiliation https://www.grid.ac/institutes/grid.411216.1
148 schema:familyName Modesto Filho
149 schema:givenName João
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014243652607.75
151 rdf:type schema:Person
152 sg:person.015041233207.00 schema:affiliation https://www.grid.ac/institutes/grid.411216.1
153 schema:familyName Alves Pegado Gomes
154 schema:givenName Cecília Neta
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015041233207.00
156 rdf:type schema:Person
157 sg:person.0754141234.45 schema:affiliation https://www.grid.ac/institutes/grid.411216.1
158 schema:familyName de Carvalho Costa
159 schema:givenName Maria José
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754141234.45
161 rdf:type schema:Person
162 sg:pub.10.1007/s11010-011-0770-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011052190
163 https://doi.org/10.1007/s11010-011-0770-0
164 rdf:type schema:CreativeWork
165 sg:pub.10.1007/s11033-013-2906-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049789268
166 https://doi.org/10.1007/s11033-013-2906-7
167 rdf:type schema:CreativeWork
168 sg:pub.10.1007/s11033-014-3729-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043027802
169 https://doi.org/10.1007/s11033-014-3729-x
170 rdf:type schema:CreativeWork
171 sg:pub.10.1007/s11239-009-0321-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026284332
172 https://doi.org/10.1007/s11239-009-0321-7
173 rdf:type schema:CreativeWork
174 sg:pub.10.1038/ng0595-111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007583916
175 https://doi.org/10.1038/ng0595-111
176 rdf:type schema:CreativeWork
177 sg:pub.10.1186/s13098-017-0284-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092270736
178 https://doi.org/10.1186/s13098-017-0284-3
179 rdf:type schema:CreativeWork
180 https://app.dimensions.ai/details/publication/pub.1075097685 schema:CreativeWork
181 https://app.dimensions.ai/details/publication/pub.1077466928 schema:CreativeWork
182 https://app.dimensions.ai/details/publication/pub.1079361788 schema:CreativeWork
183 https://doi.org/10.1016/j.arcmed.2011.12.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026264002
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/j.clon.2011.03.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008202213
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/j.jdiacomp.2006.12.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044954413
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/j.metabol.2012.10.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041433059
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/s0005-2760(98)00086-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018463813
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/s0378-1119(00)00392-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038166090
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1042/bsr20160611 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083738137
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1074/jbc.m112.431627 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012055337
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1086/301825 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058609431
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1093/jn/129.9.1656 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074511821
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1093/nar/16.3.1215 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012912024
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1136/jmg.2005.032656 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038792307
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1139/o79-007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053084481
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1177/1470320312444651 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037976186
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1177/1470320313516172 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044842297
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1371/journal.pone.0026747 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046040082
214 rdf:type schema:CreativeWork
215 https://doi.org/10.2337/diacare.23.12.1816 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009413395
216 rdf:type schema:CreativeWork
217 https://doi.org/10.3390/nu7085303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034621243
218 rdf:type schema:CreativeWork
219 https://doi.org/10.6026/97320630009929 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017878121
220 rdf:type schema:CreativeWork
221 https://www.grid.ac/institutes/grid.411216.1 schema:alternateName Federal University of Paraíba
222 schema:name Department of Internal Medicine, Federal University of Paraiba, Joao Pessoa, Brazil
223 Department of Molecular Biology and Post-Graduate Program in Nutrition Science, Federal University of Paraiba, CEP 58051-900, João Pessoa, PB, Brazil
224 Department of Molecular Biology, Federal University of Paraiba, Joao Pessoa, Brazil
225 Development and Technological Innovation of Medicines, Federal University of Paraiba, João Pessoa, Brazil
226 Nephrology Clinic, Lauro Wanderley University Hospital, Federal University of Paraiba, Joao Pessoa, Brazil
227 Nutrition Science Department and Post-Graduate Program in Nutrition Science, Federal University of Paraiba, Joao Pessoa, Brazil
228 Nutrition Sciences, Federal University of Paraiba, Joao Pessoa, Brazil
229 Post-Graduate Program in Nutrition Science, Federal University of Paraiba, Joao Pessoa, Brazil
230 Scientific Initiation Program, Federal University of Paraiba, Joao Pessoa, Brazil
231 Unidade da Visão, Hospital Universitário Lauro Wanderley, Federal University of Paraíba, Joao Pessoa, Brazil
232 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...