Local intra-articular injection of rapamycin delays articular cartilage degeneration in a murine model of osteoarthritis View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-12

AUTHORS

Koji Takayama, Yohei Kawakami, Makoto Kobayashi, Nick Greco, James H Cummins, Takehiko Matsushita, Ryosuke Kuroda, Masahiro Kurosaka, Freddie H Fu, Johnny Huard

ABSTRACT

INTRODUCTION: Recent studies have revealed that rapamycin activates autophagy in human chondrocytes preventing the development of osteoarthritis (OA) like changes in vitro, while the systemic injection of rapamycin reduces the severity of experimental osteoarthritis in a murine model of OA in vivo. Since the systemic use of rapamycin is associated with numerous side effects, the goal of the current study was to examine the beneficial effect of local intra-articular injection of rapamycin in a murine model of OA and to elucidate the mechanism of action of rapamycin on articular cartilage. METHODS: Destabilization of the medial meniscus (DMM) was performed on 10-week-old male mice to induce OA. Intra-articular injections of 10 μl of rapamycin (10 μM) were administered twice weekly for 8 weeks. Articular cartilage damage was analyzed by histology using a semi-quantitative scoring system at 8 and 12 weeks after surgery. Mammalian target of rapamycin (mTOR), light chain 3 (LC3), vascular endothelial growth factor (VEGF), collagen, type X alpha 1 (COL10A1), and matrix metallopeptidase 13 (MMP13) expressions were analyzed by immunohistochemistry. VEGF, COL10A1, and MMP13 expressions were further examined via quantitative RT-PCR (qPCR). RESULTS: Intra-articular injection of rapamycin significantly reduced the severity of articular cartilage degradation at 8 and 12 weeks after DMM surgery. A reduction in mTOR expression and the activation of LC3 (an autophagy marker) in the chondrocytes was observed in the rapamycin treated mice. Rapamycin treatment also reduced VEGF, COL10A1, and MMP13 expressions at 8 and 12 weeks after DMM surgery. CONCLUSION: These results demonstrate that the intra-articular injection of rapamycin could reduce mTOR expression, leading to a delay in articular cartilage degradation in our OA murine model. Our observations suggest that local intra-articular injection of rapamycin could represent a potential therapeutic approach to prevent OA. More... »

PAGES

482

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13075-014-0482-4

DOI

http://dx.doi.org/10.1186/s13075-014-0482-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1008661599

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25403236


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cartilage, Articular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disease Models, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunosuppressive Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Injections, Intra-Articular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Osteoarthritis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sirolimus", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Stem Cell Research Center, University of Pittsburgh, 15219, Pittsburgh, PA, USA", 
            "Department of Orthopaedic Surgery, University of Pittsburgh, 15260, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Takayama", 
        "givenName": "Koji", 
        "id": "sg:person.0602131621.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602131621.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Stem Cell Research Center, University of Pittsburgh, 15219, Pittsburgh, PA, USA", 
            "Department of Orthopaedic Surgery, University of Pittsburgh, 15260, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kawakami", 
        "givenName": "Yohei", 
        "id": "sg:person.01347361670.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347361670.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Stem Cell Research Center, University of Pittsburgh, 15219, Pittsburgh, PA, USA", 
            "Department of Orthopaedic Surgery, University of Pittsburgh, 15260, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kobayashi", 
        "givenName": "Makoto", 
        "id": "sg:person.0660106520.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660106520.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Stem Cell Research Center, University of Pittsburgh, 15219, Pittsburgh, PA, USA", 
            "Department of Orthopaedic Surgery, University of Pittsburgh, 15260, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Greco", 
        "givenName": "Nick", 
        "id": "sg:person.01214217530.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01214217530.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Department of Orthopaedic Surgery, University of Pittsburgh, 15260, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cummins", 
        "givenName": "James H", 
        "id": "sg:person.0702520553.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702520553.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kobe University", 
          "id": "https://www.grid.ac/institutes/grid.31432.37", 
          "name": [
            "Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, 650-0017, Kobe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matsushita", 
        "givenName": "Takehiko", 
        "id": "sg:person.01056105637.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01056105637.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kobe University", 
          "id": "https://www.grid.ac/institutes/grid.31432.37", 
          "name": [
            "Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, 650-0017, Kobe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kuroda", 
        "givenName": "Ryosuke", 
        "id": "sg:person.01025022257.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025022257.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kobe University", 
          "id": "https://www.grid.ac/institutes/grid.31432.37", 
          "name": [
            "Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, 650-0017, Kobe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kurosaka", 
        "givenName": "Masahiro", 
        "id": "sg:person.0646741520.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646741520.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Stem Cell Research Center, University of Pittsburgh, 15219, Pittsburgh, PA, USA", 
            "Department of Orthopaedic Surgery, University of Pittsburgh, 15260, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fu", 
        "givenName": "Freddie H", 
        "id": "sg:person.0706577466.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706577466.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pittsburgh", 
          "id": "https://www.grid.ac/institutes/grid.21925.3d", 
          "name": [
            "Stem Cell Research Center, University of Pittsburgh, 15219, Pittsburgh, PA, USA", 
            "Department of Orthopaedic Surgery, University of Pittsburgh, 15260, Pittsburgh, PA, USA", 
            "Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, School of medicine, 450 Technology Drive, Bridgeside Point II, Suite 206, 15219, Pittsburgh, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huard", 
        "givenName": "Johnny", 
        "id": "sg:person.0603751545.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603751545.18"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1042/bj20041636", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004120010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20041636", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004120010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/art.33444", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004643549"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007890-200405150-00002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005016963"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007890-200405150-00002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005016963"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1209990", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008091156", 
          "https://doi.org/10.1038/sj.onc.1209990"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1209990", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008091156", 
          "https://doi.org/10.1038/sj.onc.1209990"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/art.24225", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008263485"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m109.056838", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008288904"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4402120", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009439832", 
          "https://doi.org/10.1038/sj.cdd.4402120"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cdd.4402120", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009439832", 
          "https://doi.org/10.1038/sj.cdd.4402120"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0169-6009(92)90851-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009720369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2474-10-166", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012508828", 
          "https://doi.org/10.1186/1471-2474-10-166"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(09)60709-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012551394"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/art.34323", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013670928"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/art.24443", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013984680"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/art.24443", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013984680"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0202-128", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015189512", 
          "https://doi.org/10.1038/nm0202-128"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0202-128", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015189512", 
          "https://doi.org/10.1038/nm0202-128"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2007.12.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015281436"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.joca.2011.11.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017307030"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.joca.2010.04.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018018752"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00467-007-0456-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023329790", 
          "https://doi.org/10.1007/s00467-007-0456-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00467-007-0456-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023329790", 
          "https://doi.org/10.1007/s00467-007-0456-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature06639", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025718085", 
          "https://doi.org/10.1038/nature06639"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/annrheumdis-2011-200557", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026439690"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.cir.0000130641.08705.45", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027905907"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0177(199607)206:3<260::aid-aja4>3.0.co;2-g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028033983"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m511621200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030584444"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1359/jbmr.2000.15.1.82", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030748552"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamcr.2008.07.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033100595"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jbspin.2010.07.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037203213"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa066838", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037798278"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01657", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038561598", 
          "https://doi.org/10.1038/nature01657"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01657", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038561598", 
          "https://doi.org/10.1038/nature01657"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1534-5807(04)00099-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040769254"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.joca.2007.03.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042632632"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.joca.2011.12.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043267875"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.yexcr.2005.05.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044471210"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.joca.2013.08.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045901564"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc1362", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046070024", 
          "https://doi.org/10.1038/nrc1362"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc1362", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046070024", 
          "https://doi.org/10.1038/nrc1362"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ki.2010.173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047971822", 
          "https://doi.org/10.1038/ki.2010.173"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2005.12.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050656487"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/art.24774", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051424371"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/art.24774", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051424371"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.joca.2010.05.025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051478671"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00167-012-2333-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053629202", 
          "https://doi.org/10.1007/s00167-012-2333-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00003086-197409000-00088", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060156876"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00003086-197409000-00088", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060156876"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00003086-197409000-00088", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060156876"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-12", 
    "datePublishedReg": "2014-12-01", 
    "description": "INTRODUCTION: Recent studies have revealed that rapamycin activates autophagy in human chondrocytes preventing the development of osteoarthritis (OA) like changes in vitro, while the systemic injection of rapamycin reduces the severity of experimental osteoarthritis in a murine model of OA in vivo. Since the systemic use of rapamycin is associated with numerous side effects, the goal of the current study was to examine the beneficial effect of local intra-articular injection of rapamycin in a murine model of OA and to elucidate the mechanism of action of rapamycin on articular cartilage.\nMETHODS: Destabilization of the medial meniscus (DMM) was performed on 10-week-old male mice to induce OA. Intra-articular injections of 10\u00a0\u03bcl of rapamycin (10\u00a0\u03bcM) were administered twice weekly for 8\u00a0weeks. Articular cartilage damage was analyzed by histology using a semi-quantitative scoring system at 8 and 12\u00a0weeks after surgery. Mammalian target of rapamycin (mTOR), light chain 3 (LC3), vascular endothelial growth factor (VEGF), collagen, type X alpha 1 (COL10A1), and matrix metallopeptidase 13 (MMP13) expressions were analyzed by immunohistochemistry. VEGF, COL10A1, and MMP13 expressions were further examined via quantitative RT-PCR (qPCR).\nRESULTS: Intra-articular injection of rapamycin significantly reduced the severity of articular cartilage degradation at 8 and 12\u00a0weeks after DMM surgery. A reduction in mTOR expression and the activation of LC3 (an autophagy marker) in the chondrocytes was observed in the rapamycin treated mice. Rapamycin treatment also reduced VEGF, COL10A1, and MMP13 expressions at 8 and 12\u00a0weeks after DMM surgery.\nCONCLUSION: These results demonstrate that the intra-articular injection of rapamycin could reduce mTOR expression, leading to a delay in articular cartilage degradation in our OA murine model. Our observations suggest that local intra-articular injection of rapamycin could represent a potential therapeutic approach to prevent OA.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s13075-014-0482-4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.7132113", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6135575", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1297499", 
        "issn": [
          "1478-6354", 
          "1478-6362"
        ], 
        "name": "Arthritis Research & Therapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "16"
      }
    ], 
    "name": "Local intra-articular injection of rapamycin delays articular cartilage degeneration in a murine model of osteoarthritis", 
    "pagination": "482", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "294413e35e2f3f9db8cc87ef29d0a89be0ed52b70a856d37ef273a2f8ca1af3d"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "25403236"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101154438"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s13075-014-0482-4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1008661599"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s13075-014-0482-4", 
      "https://app.dimensions.ai/details/publication/pub.1008661599"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:11", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000367_0000000367/records_88255_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2Fs13075-014-0482-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13075-014-0482-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13075-014-0482-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13075-014-0482-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13075-014-0482-4'


 

This table displays all metadata directly associated to this object as RDF triples.

304 TRIPLES      21 PREDICATES      77 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s13075-014-0482-4 schema:about N1b21776fe08a40eaac00caa890c23aac
2 N27014b6f7c7b4f87994a7e51a06c9453
3 N2d6630d4a0ba483cbbf8de3008f47dfb
4 N2e20415afd044816baf1a5ea992ca9a5
5 N4df65927969240ffbc4dc7ad4405f497
6 N68713e424bf942d88a8e0d2ca86b80a1
7 N7906900069e54ecb99210730c6d52a8c
8 Nb1079bff8ab24f00bc87de768c5f6008
9 Nfa1de44362ff4e6993a773e79151777c
10 anzsrc-for:11
11 anzsrc-for:1103
12 schema:author N15c324eb35d942c2b426dd50ef6d68ad
13 schema:citation sg:pub.10.1007/s00167-012-2333-7
14 sg:pub.10.1007/s00467-007-0456-8
15 sg:pub.10.1038/ki.2010.173
16 sg:pub.10.1038/nature01657
17 sg:pub.10.1038/nature06639
18 sg:pub.10.1038/nm0202-128
19 sg:pub.10.1038/nrc1362
20 sg:pub.10.1038/sj.cdd.4402120
21 sg:pub.10.1038/sj.onc.1209990
22 sg:pub.10.1186/1471-2474-10-166
23 https://doi.org/10.1002/(sici)1097-0177(199607)206:3<260::aid-aja4>3.0.co;2-g
24 https://doi.org/10.1002/art.24225
25 https://doi.org/10.1002/art.24443
26 https://doi.org/10.1002/art.24774
27 https://doi.org/10.1002/art.33444
28 https://doi.org/10.1002/art.34323
29 https://doi.org/10.1016/0169-6009(92)90851-4
30 https://doi.org/10.1016/j.bbamcr.2008.07.014
31 https://doi.org/10.1016/j.bbrc.2005.12.012
32 https://doi.org/10.1016/j.cell.2007.12.018
33 https://doi.org/10.1016/j.jbspin.2010.07.001
34 https://doi.org/10.1016/j.joca.2007.03.006
35 https://doi.org/10.1016/j.joca.2010.04.012
36 https://doi.org/10.1016/j.joca.2010.05.025
37 https://doi.org/10.1016/j.joca.2011.11.015
38 https://doi.org/10.1016/j.joca.2011.12.003
39 https://doi.org/10.1016/j.joca.2013.08.018
40 https://doi.org/10.1016/j.yexcr.2005.05.017
41 https://doi.org/10.1016/s0140-6736(09)60709-1
42 https://doi.org/10.1016/s1534-5807(04)00099-1
43 https://doi.org/10.1042/bj20041636
44 https://doi.org/10.1056/nejmoa066838
45 https://doi.org/10.1074/jbc.m109.056838
46 https://doi.org/10.1074/jbc.m511621200
47 https://doi.org/10.1097/00003086-197409000-00088
48 https://doi.org/10.1097/00007890-200405150-00002
49 https://doi.org/10.1136/annrheumdis-2011-200557
50 https://doi.org/10.1161/01.cir.0000130641.08705.45
51 https://doi.org/10.1359/jbmr.2000.15.1.82
52 schema:datePublished 2014-12
53 schema:datePublishedReg 2014-12-01
54 schema:description INTRODUCTION: Recent studies have revealed that rapamycin activates autophagy in human chondrocytes preventing the development of osteoarthritis (OA) like changes in vitro, while the systemic injection of rapamycin reduces the severity of experimental osteoarthritis in a murine model of OA in vivo. Since the systemic use of rapamycin is associated with numerous side effects, the goal of the current study was to examine the beneficial effect of local intra-articular injection of rapamycin in a murine model of OA and to elucidate the mechanism of action of rapamycin on articular cartilage. METHODS: Destabilization of the medial meniscus (DMM) was performed on 10-week-old male mice to induce OA. Intra-articular injections of 10 μl of rapamycin (10 μM) were administered twice weekly for 8 weeks. Articular cartilage damage was analyzed by histology using a semi-quantitative scoring system at 8 and 12 weeks after surgery. Mammalian target of rapamycin (mTOR), light chain 3 (LC3), vascular endothelial growth factor (VEGF), collagen, type X alpha 1 (COL10A1), and matrix metallopeptidase 13 (MMP13) expressions were analyzed by immunohistochemistry. VEGF, COL10A1, and MMP13 expressions were further examined via quantitative RT-PCR (qPCR). RESULTS: Intra-articular injection of rapamycin significantly reduced the severity of articular cartilage degradation at 8 and 12 weeks after DMM surgery. A reduction in mTOR expression and the activation of LC3 (an autophagy marker) in the chondrocytes was observed in the rapamycin treated mice. Rapamycin treatment also reduced VEGF, COL10A1, and MMP13 expressions at 8 and 12 weeks after DMM surgery. CONCLUSION: These results demonstrate that the intra-articular injection of rapamycin could reduce mTOR expression, leading to a delay in articular cartilage degradation in our OA murine model. Our observations suggest that local intra-articular injection of rapamycin could represent a potential therapeutic approach to prevent OA.
55 schema:genre research_article
56 schema:inLanguage en
57 schema:isAccessibleForFree true
58 schema:isPartOf Ncce6b507529f4809a635ed1c684d1c45
59 Nedd42f2e4b914c2aae5807910aea600f
60 sg:journal.1297499
61 schema:name Local intra-articular injection of rapamycin delays articular cartilage degeneration in a murine model of osteoarthritis
62 schema:pagination 482
63 schema:productId N19631f1076e24c7488142d52fa36b194
64 N5b90fabfb93e47db9b1277b72e1ffd20
65 N70ce281a51334390a8d41b5b83853e5e
66 Ne58c6a38d5ee48658a831764801270d9
67 Nfd2791086aec4f729f4c752d662ef6e5
68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008661599
69 https://doi.org/10.1186/s13075-014-0482-4
70 schema:sdDatePublished 2019-04-11T13:11
71 schema:sdLicense https://scigraph.springernature.com/explorer/license/
72 schema:sdPublisher N41897f53b71f4fb9882f533c27f23740
73 schema:url http://link.springer.com/10.1186%2Fs13075-014-0482-4
74 sgo:license sg:explorer/license/
75 sgo:sdDataset articles
76 rdf:type schema:ScholarlyArticle
77 N056e0cbaad7147cf887ac140bbefd663 rdf:first sg:person.01214217530.53
78 rdf:rest N13253bcaeee24b7fbd1c71691fb34cfb
79 N08bcd295bac64803b6972017989457bf rdf:first sg:person.0603751545.18
80 rdf:rest rdf:nil
81 N13253bcaeee24b7fbd1c71691fb34cfb rdf:first sg:person.0702520553.08
82 rdf:rest Nde27c48cfe744474ae4aaadefea268a7
83 N15c324eb35d942c2b426dd50ef6d68ad rdf:first sg:person.0602131621.32
84 rdf:rest Nddece936ab9d4adeb53023fb0f0e6213
85 N19631f1076e24c7488142d52fa36b194 schema:name nlm_unique_id
86 schema:value 101154438
87 rdf:type schema:PropertyValue
88 N1b21776fe08a40eaac00caa890c23aac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Mice
90 rdf:type schema:DefinedTerm
91 N27014b6f7c7b4f87994a7e51a06c9453 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Disease Models, Animal
93 rdf:type schema:DefinedTerm
94 N2d6630d4a0ba483cbbf8de3008f47dfb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Male
96 rdf:type schema:DefinedTerm
97 N2e20415afd044816baf1a5ea992ca9a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Osteoarthritis
99 rdf:type schema:DefinedTerm
100 N35362ca0f88249ea950c20c856b88587 rdf:first sg:person.0646741520.97
101 rdf:rest Ne6cef1f6ab0446f29cc42941cee775bc
102 N41897f53b71f4fb9882f533c27f23740 schema:name Springer Nature - SN SciGraph project
103 rdf:type schema:Organization
104 N4df65927969240ffbc4dc7ad4405f497 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Injections, Intra-Articular
106 rdf:type schema:DefinedTerm
107 N5b90fabfb93e47db9b1277b72e1ffd20 schema:name doi
108 schema:value 10.1186/s13075-014-0482-4
109 rdf:type schema:PropertyValue
110 N68713e424bf942d88a8e0d2ca86b80a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Immunosuppressive Agents
112 rdf:type schema:DefinedTerm
113 N70ce281a51334390a8d41b5b83853e5e schema:name pubmed_id
114 schema:value 25403236
115 rdf:type schema:PropertyValue
116 N7906900069e54ecb99210730c6d52a8c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Cartilage, Articular
118 rdf:type schema:DefinedTerm
119 N9c94ea43f85a48edb5d8f948f8967f9f rdf:first sg:person.0660106520.42
120 rdf:rest N056e0cbaad7147cf887ac140bbefd663
121 Nb1079bff8ab24f00bc87de768c5f6008 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Sirolimus
123 rdf:type schema:DefinedTerm
124 Ncce6b507529f4809a635ed1c684d1c45 schema:issueNumber 6
125 rdf:type schema:PublicationIssue
126 Nddece936ab9d4adeb53023fb0f0e6213 rdf:first sg:person.01347361670.25
127 rdf:rest N9c94ea43f85a48edb5d8f948f8967f9f
128 Nde27c48cfe744474ae4aaadefea268a7 rdf:first sg:person.01056105637.26
129 rdf:rest Nfaeeaf173f61462d888d35fde5114603
130 Ne58c6a38d5ee48658a831764801270d9 schema:name dimensions_id
131 schema:value pub.1008661599
132 rdf:type schema:PropertyValue
133 Ne6cef1f6ab0446f29cc42941cee775bc rdf:first sg:person.0706577466.24
134 rdf:rest N08bcd295bac64803b6972017989457bf
135 Nedd42f2e4b914c2aae5807910aea600f schema:volumeNumber 16
136 rdf:type schema:PublicationVolume
137 Nfa1de44362ff4e6993a773e79151777c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Animals
139 rdf:type schema:DefinedTerm
140 Nfaeeaf173f61462d888d35fde5114603 rdf:first sg:person.01025022257.04
141 rdf:rest N35362ca0f88249ea950c20c856b88587
142 Nfd2791086aec4f729f4c752d662ef6e5 schema:name readcube_id
143 schema:value 294413e35e2f3f9db8cc87ef29d0a89be0ed52b70a856d37ef273a2f8ca1af3d
144 rdf:type schema:PropertyValue
145 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
146 schema:name Medical and Health Sciences
147 rdf:type schema:DefinedTerm
148 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
149 schema:name Clinical Sciences
150 rdf:type schema:DefinedTerm
151 sg:grant.6135575 http://pending.schema.org/fundedItem sg:pub.10.1186/s13075-014-0482-4
152 rdf:type schema:MonetaryGrant
153 sg:grant.7132113 http://pending.schema.org/fundedItem sg:pub.10.1186/s13075-014-0482-4
154 rdf:type schema:MonetaryGrant
155 sg:journal.1297499 schema:issn 1478-6354
156 1478-6362
157 schema:name Arthritis Research & Therapy
158 rdf:type schema:Periodical
159 sg:person.01025022257.04 schema:affiliation https://www.grid.ac/institutes/grid.31432.37
160 schema:familyName Kuroda
161 schema:givenName Ryosuke
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025022257.04
163 rdf:type schema:Person
164 sg:person.01056105637.26 schema:affiliation https://www.grid.ac/institutes/grid.31432.37
165 schema:familyName Matsushita
166 schema:givenName Takehiko
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01056105637.26
168 rdf:type schema:Person
169 sg:person.01214217530.53 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
170 schema:familyName Greco
171 schema:givenName Nick
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01214217530.53
173 rdf:type schema:Person
174 sg:person.01347361670.25 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
175 schema:familyName Kawakami
176 schema:givenName Yohei
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01347361670.25
178 rdf:type schema:Person
179 sg:person.0602131621.32 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
180 schema:familyName Takayama
181 schema:givenName Koji
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602131621.32
183 rdf:type schema:Person
184 sg:person.0603751545.18 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
185 schema:familyName Huard
186 schema:givenName Johnny
187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603751545.18
188 rdf:type schema:Person
189 sg:person.0646741520.97 schema:affiliation https://www.grid.ac/institutes/grid.31432.37
190 schema:familyName Kurosaka
191 schema:givenName Masahiro
192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646741520.97
193 rdf:type schema:Person
194 sg:person.0660106520.42 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
195 schema:familyName Kobayashi
196 schema:givenName Makoto
197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660106520.42
198 rdf:type schema:Person
199 sg:person.0702520553.08 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
200 schema:familyName Cummins
201 schema:givenName James H
202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702520553.08
203 rdf:type schema:Person
204 sg:person.0706577466.24 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
205 schema:familyName Fu
206 schema:givenName Freddie H
207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706577466.24
208 rdf:type schema:Person
209 sg:pub.10.1007/s00167-012-2333-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053629202
210 https://doi.org/10.1007/s00167-012-2333-7
211 rdf:type schema:CreativeWork
212 sg:pub.10.1007/s00467-007-0456-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023329790
213 https://doi.org/10.1007/s00467-007-0456-8
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/ki.2010.173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047971822
216 https://doi.org/10.1038/ki.2010.173
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/nature01657 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038561598
219 https://doi.org/10.1038/nature01657
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/nature06639 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025718085
222 https://doi.org/10.1038/nature06639
223 rdf:type schema:CreativeWork
224 sg:pub.10.1038/nm0202-128 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015189512
225 https://doi.org/10.1038/nm0202-128
226 rdf:type schema:CreativeWork
227 sg:pub.10.1038/nrc1362 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046070024
228 https://doi.org/10.1038/nrc1362
229 rdf:type schema:CreativeWork
230 sg:pub.10.1038/sj.cdd.4402120 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009439832
231 https://doi.org/10.1038/sj.cdd.4402120
232 rdf:type schema:CreativeWork
233 sg:pub.10.1038/sj.onc.1209990 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008091156
234 https://doi.org/10.1038/sj.onc.1209990
235 rdf:type schema:CreativeWork
236 sg:pub.10.1186/1471-2474-10-166 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012508828
237 https://doi.org/10.1186/1471-2474-10-166
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1002/(sici)1097-0177(199607)206:3<260::aid-aja4>3.0.co;2-g schema:sameAs https://app.dimensions.ai/details/publication/pub.1028033983
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1002/art.24225 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008263485
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1002/art.24443 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013984680
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1002/art.24774 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051424371
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1002/art.33444 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004643549
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1002/art.34323 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013670928
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1016/0169-6009(92)90851-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009720369
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1016/j.bbamcr.2008.07.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033100595
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1016/j.bbrc.2005.12.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050656487
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1016/j.cell.2007.12.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015281436
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1016/j.jbspin.2010.07.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037203213
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1016/j.joca.2007.03.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042632632
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1016/j.joca.2010.04.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018018752
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1016/j.joca.2010.05.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051478671
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1016/j.joca.2011.11.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017307030
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1016/j.joca.2011.12.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043267875
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1016/j.joca.2013.08.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045901564
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1016/j.yexcr.2005.05.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044471210
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1016/s0140-6736(09)60709-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012551394
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1016/s1534-5807(04)00099-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040769254
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1042/bj20041636 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004120010
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1056/nejmoa066838 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037798278
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1074/jbc.m109.056838 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008288904
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1074/jbc.m511621200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030584444
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1097/00003086-197409000-00088 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060156876
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1097/00007890-200405150-00002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005016963
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1136/annrheumdis-2011-200557 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026439690
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1161/01.cir.0000130641.08705.45 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027905907
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1359/jbmr.2000.15.1.82 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030748552
296 rdf:type schema:CreativeWork
297 https://www.grid.ac/institutes/grid.21925.3d schema:alternateName University of Pittsburgh
298 schema:name Department of Orthopaedic Surgery, University of Pittsburgh, 15260, Pittsburgh, PA, USA
299 Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, School of medicine, 450 Technology Drive, Bridgeside Point II, Suite 206, 15219, Pittsburgh, PA, USA
300 Stem Cell Research Center, University of Pittsburgh, 15219, Pittsburgh, PA, USA
301 rdf:type schema:Organization
302 https://www.grid.ac/institutes/grid.31432.37 schema:alternateName Kobe University
303 schema:name Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, 650-0017, Kobe, Japan
304 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...