Neem oil increases the persistence of the entomopathogenic fungus Metarhizium anisopliae for the control of Aedes aegypti (Diptera: Culicidae) larvae View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-04-11

AUTHORS

Adriano R. Paula, Anderson Ribeiro, Francisco José Alves Lemos, Carlos P. Silva, Richard I. Samuels

ABSTRACT

BACKGROUND: The entomopathogenic fungus Metarhizium anisopliae is a candidate for the integrated management of the disease vector mosquito Aedes aegypti. Metarhizium anisopliae is pathogenic and virulent against Ae. aegypti larvae; however, its half-life is short without employing adjuvants. Here, we investigated the use of neem oil to increase virulence and persistence of the fungus under laboratory and simulated field conditions. METHODS: Neem was mixed with M. anisopliae and added to recipients. Larvae were then placed in recipients at 5-day intervals for up to 50 days. Survival rates were evaluated 7 days after exposing larvae to each treatment. The effect of neem on conidial germination following exposure to ultraviolet radiation was evaluated under laboratory conditions. Statistical tests were carried out using ANOVA and regression analysis. RESULTS: Laboratory bioassays showed that the fungus alone reduced survival to 30% when larvae were exposed to the treatment as soon as the suspension had been prepared (time zero). A mixture of fungus + neem resulted in 11% survival at time zero. The combination of fungus + neem significantly reduced larval survival rates even when suspensions had been maintained for up to 45 days before adding larvae. For simulated-field experiments 1% neem was used, even though this concentration is insecticidal, resulting in 20% survival at time zero. However, this toxic effect was reduced over time. When used alone under simulated-field conditions the fungus rapidly lost virulence. The formulation fungus + neem effectively maintained fungal virulence, with larval survival rates significantly reduced for up to 45 days after preparation of the suspensions. The effective half-life of the fungus or neem when used separately was 6 and 13 days, respectively. The half-life of fungus formulated in 1% neem was 34 days. Conidia suspended in neem maintained high levels of germination even following a 2-h exposure to ultraviolet radiation. CONCLUSIONS: A combination of the entomopathogenic fungus M. anisopliae with neem oil effectively increases the half-life and virulence of the fungus when tested against Ae. aegypti larvae, even under simulated field conditions. Neem oil also protected the fungus from the damaging effects of ultraviolet radiation. More... »

PAGES

163

References to SciGraph publications

  • 2007-04-03. Selection of entomopathogenic fungi for use in combination with sub-lethal doses of imidacloprid: perspectives for the control of the leaf-cutting ant Atta sexdensrubropilosa Forel (Hymenoptera: Formicidae) in MYCOPATHOLOGIA
  • 2015-05-19. Tolerance of entomopathogenic fungi to ultraviolet radiation: a review on screening of strains and their formulation in CURRENT GENETICS
  • 2014-08-06. Compatibility between entomopathogenic fungi and biorational insecticides in toxicity against Ronderosia bergi under laboratory conditions in BIOCONTROL
  • 2007-05-22. Larvicidal effects of a neem (Azadirachta indica) oil formulation on the malaria vector Anopheles gambiae in MALARIA JOURNAL
  • 2011-02-22. Development of Metarhizium anisopliae and Beauveria bassiana formulations for control of malaria mosquito larvae in PARASITES & VECTORS
  • 2004-09. Mediation of oviposition site selection in the African malaria mosquito Anopheles gambiae (Diptera: Culicidae) by semiochemicals of microbial origin in INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE
  • 2009-06-08. Larvicidal activity of neem oil (Azadirachta indica) formulation against mosquitoes in MALARIA JOURNAL
  • 2005-08. Compatibility of the fungus Beauveria Bassiana (Bals.) Vuill. (Deuteromycetes) with extracts of neem seeds and leaves and the emulsible oil in NEOTROPICAL ENTOMOLOGY
  • 2013-09-08. Testing fungus impregnated cloths for the control of adult Aedes aegypti under natural conditions in PARASITES & VECTORS
  • 2015-12-30. Neem oil increases the efficiency of the entomopathogenic fungus Metarhizium anisopliae for the control of Aedes aegypti (Diptera: Culicidae) larvae in PARASITES & VECTORS
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    http://scigraph.springernature.com/pub.10.1186/s13071-019-3415-x

    DOI

    http://dx.doi.org/10.1186/s13071-019-3415-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1113377920

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30975207


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