Relationship between insecticide resistance and kdr mutations in the dengue vector Aedes aegypti in Southern China View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-12

AUTHORS

Chun-Xiao Li, Phillip E Kaufman, Rui-De Xue, Ming-Hui Zhao, Gang Wang, Ting Yan, Xiao-Xia Guo, Ying-Mei Zhang, Yan-De Dong, Dan Xing, Heng-Duan Zhang, Tong-Yan Zhao

ABSTRACT

BACKGROUND: Aedes aegypti is an important vector for dengue virus and thus has been targeted with pyrethroid insecticides in many areas of the world. As such, resistance has been detected to several of these insecticides, including in China, but the mechanisms of the resistance are not well understood in this country. METHODS: Using the World Health Organization larval mosquito bioassay, five field populations of Aedes aegypti from Southern China were characterized for their resistance to cypermethrin and cyhalothrin. RNA extraction with PCR amplification, cloning and sequencing of the sodium channel gene was followed by comparisons of susceptible and wild mosquito strains Additionally, genomic DNA was used for Allele-specific PCR (AS-PCR) genotyping of the sodium channel genes to detect S989P, V1016G and F1534C mutations and allow for correlation analysis of resistance expression for the different mutations. RESULTS: All wild strains expressed resistance to cypermethrin and cyhalothrin and the resistance expression between the two insecticides was highly correlated suggesting cross-resistance between these two pyrethroids. The AS-PCR technique effectively distinguished individual genotypes for all three mutations. Among the five wild strains tested, two strains carried all three mutations. Although the S989P and V1016G mutations were positively correlated to resistance expression of both pyrethroids, the F1534C mutation was negatively correlated. CONCLUSIONS: Our methodology proved highly reliable and will aid future detection of kdr mutations. The three sodium channel mutations were common in the Ae. aegypti strains sampled from Southern China. The V1016G mutation appears to be the most important kdr mutation in Ae. aegypti strains in Southern China. More... »

PAGES

325

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13071-015-0933-z

DOI

http://dx.doi.org/10.1186/s13071-015-0933-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051843981

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26068925


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HOW TO GET THIS DATA PROGRAMMATICALLY:

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curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13071-015-0933-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13071-015-0933-z'

Turtle is a human-readable linked data format.

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RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13071-015-0933-z'


 

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