Application of qPCR in conjunctival swab samples for the evaluation of canine leishmaniasis in borderline cases or disease relapse and ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-12

AUTHORS

Marcello Ceccarelli, Luca Galluzzi, Davide Sisti, Barbara Bianchi, Mauro Magnani

ABSTRACT

BACKGROUND: In leishmaniasis caused by Leishmania infantum, the dog acts as the main reservoir for the disease. Non-invasive sampling for Leishmania detection is pivotal for rapid and affordable diagnosis. Recently, the use of conjunctival swab (CS) has been evaluated as a non-invasive sampling technique for quantitative real-time PCR (qPCR). However, few investigations have been made on the applicability of CS qPCR in particular cases such as dogs with borderline IFAT titres, suspected disease relapse with comorbidity and therapy monitoring. The aims of this study were i) to confirm the efficacy of CS, comparing these samples to buffy coat (BC) samples, as effective non-invasive samples for Leishmania quantitative detection by qPCR and ii) to verify the usefulness of qPCR compared to conventional laboratory and clinical parameters to assist in therapeutic decision making regarding dogs with complex clinical cases. METHODS: Eighty dogs were divided into 4 groups based on their IFAT titres and clinical histories. Two qPCR assays were performed both on CS raw lysates and on purified DNA from BC samples. The assays were then compared. Z tests for difference of proportion, with Bonferroni correction, were carried out to evaluate the qPCR results. Logistic regression with backward stepwise elimination was performed to detect the subset of haematochemical variables significantly associated with PCR positivity. RESULTS: The qPCR performed on CS samples showed better sensitivity (87%) and specificity (96%) than assays carried out using BC samples, regardless of the primers used. The haematochemical parameters haemoglobin and globulins were found to be significantly associated with qPCR positivity. Pearson correlations between Leishmania kDNA load in CS and body condition scores or IFAT titres were calculated in dogs with new leishmaniasis diagnoses. The Leishmania kDNA load in CS correlated moderately with IFAT titres (R = 0.59) but a very weak correlation (R = 0.37) with body condition score (BCS) was found. CONCLUSIONS: The applicability of CS for Leishmania detection in dogs was confirmed, revealing the usefulness of raw lysates for quantitative purposes. Moreover, the qPCR was found to be particularly useful in cases lacking a clear clinical diagnosis, where the haematochemical values cannot be predictive. More... »

PAGES

460

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13071-014-0460-3

DOI

http://dx.doi.org/10.1186/s13071-014-0460-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1053214130

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25331737


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antiprotozoal Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Conjunctiva", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dog Diseases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dogs", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leishmaniasis, Visceral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recurrence", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Urbino", 
          "id": "https://www.grid.ac/institutes/grid.12711.34", 
          "name": [
            "Department of Biomolecular Sciences, University of Urbino \u201cCarlo Bo\u201d, via Saffi 2, 61029, Urbino, PU, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ceccarelli", 
        "givenName": "Marcello", 
        "id": "sg:person.01101522732.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101522732.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Urbino", 
          "id": "https://www.grid.ac/institutes/grid.12711.34", 
          "name": [
            "Department of Biomolecular Sciences, University of Urbino \u201cCarlo Bo\u201d, via Saffi 2, 61029, Urbino, PU, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Galluzzi", 
        "givenName": "Luca", 
        "id": "sg:person.0617701361.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617701361.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Urbino", 
          "id": "https://www.grid.ac/institutes/grid.12711.34", 
          "name": [
            "Department of Biomolecular Sciences, University of Urbino \u201cCarlo Bo\u201d, via Saffi 2, 61029, Urbino, PU, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sisti", 
        "givenName": "Davide", 
        "id": "sg:person.01331435666.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01331435666.80"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Veterinary Clinic \u201cSanta Teresa\u201d, via Piave 23, 61032, Fano, PU, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bianchi", 
        "givenName": "Barbara", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Urbino", 
          "id": "https://www.grid.ac/institutes/grid.12711.34", 
          "name": [
            "Department of Biomolecular Sciences, University of Urbino \u201cCarlo Bo\u201d, via Saffi 2, 61029, Urbino, PU, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Magnani", 
        "givenName": "Mauro", 
        "id": "sg:person.0752252542.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752252542.98"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1371/journal.pone.0088845", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000320145"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pntd.0001955", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002506093"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1756-3305-4-86", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004739227", 
          "https://doi.org/10.1186/1756-3305-4-86"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.39.2.613-617.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007412134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jcpa.2007.09.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009764493"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1007526401175", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009777883", 
          "https://doi.org/10.1023/a:1007526401175"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.40.1.210-215.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012163138"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pntd.0001596", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012642431"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jac/dkr185", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014130960"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetpar.2011.08.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014283057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/cdli.9.5.1137-1141.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014499409"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0304-4017(97)00208-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017215367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0103635", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020871602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0091009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022084754"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.rvsc.2011.01.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022257053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetpar.2010.03.025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026119281"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biopsych.2005.09.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026396817"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/383281", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027085287"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4269/ajtmh.2010.09-0366", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029306635"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetpar.2012.10.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029856185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0035671", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033992319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.00558-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036249656"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.rvsc.2009.02.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036576844"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1756-3305-5-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037470936", 
          "https://doi.org/10.1186/1756-3305-5-15"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0035-9203(92)90295-n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037529058"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tvjl.2013.07.025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041981153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0304-4017(03)00061-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043119459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0304-4017(03)00061-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043119459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetpar.2009.05.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046148433"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetpar.2008.07.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046598503"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.micinf.2007.06.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047612873"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tvjl.2007.04.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049931092"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.42.11.5249-5255.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050729371"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074507808", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4269/ajtmh.2006.75.858", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077327855"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00937115", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082500267", 
          "https://doi.org/10.1007/bf00937115"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4269/ajtmh.1996.55.273", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082972767"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-12", 
    "datePublishedReg": "2014-12-01", 
    "description": "BACKGROUND: In leishmaniasis caused by Leishmania infantum, the dog acts as the main reservoir for the disease. Non-invasive sampling for Leishmania detection is pivotal for rapid and affordable diagnosis. Recently, the use of conjunctival swab (CS) has been evaluated as a non-invasive sampling technique for quantitative real-time PCR (qPCR). However, few investigations have been made on the applicability of CS qPCR in particular cases such as dogs with borderline IFAT titres, suspected disease relapse with comorbidity and therapy monitoring. The aims of this study were i) to confirm the efficacy of CS, comparing these samples to buffy coat (BC) samples, as effective non-invasive samples for Leishmania quantitative detection by qPCR and ii) to verify the usefulness of qPCR compared to conventional laboratory and clinical parameters to assist in therapeutic decision making regarding dogs with complex clinical cases.\nMETHODS: Eighty dogs were divided into 4 groups based on their IFAT titres and clinical histories. Two qPCR assays were performed both on CS raw lysates and on purified DNA from BC samples. The assays were then compared. Z tests for difference of proportion, with Bonferroni correction, were carried out to evaluate the qPCR results. Logistic regression with backward stepwise elimination was performed to detect the subset of haematochemical variables significantly associated with PCR positivity.\nRESULTS: The qPCR performed on CS samples showed better sensitivity (87%) and specificity (96%) than assays carried out using BC samples, regardless of the primers used. The haematochemical parameters haemoglobin and globulins were found to be significantly associated with qPCR positivity. Pearson correlations between Leishmania kDNA load in CS and body condition scores or IFAT titres were calculated in dogs with new leishmaniasis diagnoses. The Leishmania kDNA load in CS correlated moderately with IFAT titres (R\u2009=\u20090.59) but a very weak correlation (R\u2009=\u20090.37) with body condition score (BCS) was found.\nCONCLUSIONS: The applicability of CS for Leishmania detection in dogs was confirmed, revealing the usefulness of raw lysates for quantitative purposes. Moreover, the qPCR was found to be particularly useful in cases lacking a clear clinical diagnosis, where the haematochemical values cannot be predictive.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s13071-014-0460-3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1039458", 
        "issn": [
          "1756-3305"
        ], 
        "name": "Filaria Journal", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "name": "Application of qPCR in conjunctival swab samples for the evaluation of canine leishmaniasis in borderline cases or disease relapse and correlation with clinical parameters", 
    "pagination": "460", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "fd7879920ed9af88b881099b0c827b3ae86d6fe502525c3a4b1181092c45dad6"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "25331737"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101462774"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s13071-014-0460-3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1053214130"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s13071-014-0460-3", 
      "https://app.dimensions.ai/details/publication/pub.1053214130"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:10", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000367_0000000367/records_88242_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2Fs13071-014-0460-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13071-014-0460-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13071-014-0460-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13071-014-0460-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13071-014-0460-3'


 

This table displays all metadata directly associated to this object as RDF triples.

248 TRIPLES      21 PREDICATES      75 URIs      31 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s13071-014-0460-3 schema:about N00dd7e8e86974d16aaf233366a84788e
2 N14fef08003444781a81f732b899de329
3 N22885372a82f4a96a6397155593af5d6
4 N3b114752dc4b4f2397d04e06d7267ef0
5 N5e739e414ebf47e690ac07b06c9019b0
6 N82bdd5e901bc4c3dacee632a770e0665
7 Na560a7ea18ec4515af68ae1053c151e8
8 Nabcee16b8fc949d8bebaa9b73f63c701
9 Nb7f28eb8cfd14ce4bd2c9e5eedba2275
10 Nc2df932c6c174444b7f0dc1fb70dffdf
11 anzsrc-for:11
12 anzsrc-for:1103
13 schema:author Nb462d52003d043759bf2ad8fb111051b
14 schema:citation sg:pub.10.1007/bf00937115
15 sg:pub.10.1023/a:1007526401175
16 sg:pub.10.1186/1756-3305-4-86
17 sg:pub.10.1186/1756-3305-5-15
18 https://app.dimensions.ai/details/publication/pub.1074507808
19 https://doi.org/10.1016/0035-9203(92)90295-n
20 https://doi.org/10.1016/j.biopsych.2005.09.014
21 https://doi.org/10.1016/j.jcpa.2007.09.004
22 https://doi.org/10.1016/j.micinf.2007.06.005
23 https://doi.org/10.1016/j.rvsc.2009.02.005
24 https://doi.org/10.1016/j.rvsc.2011.01.004
25 https://doi.org/10.1016/j.tvjl.2007.04.013
26 https://doi.org/10.1016/j.tvjl.2013.07.025
27 https://doi.org/10.1016/j.vetpar.2008.07.028
28 https://doi.org/10.1016/j.vetpar.2009.05.022
29 https://doi.org/10.1016/j.vetpar.2010.03.025
30 https://doi.org/10.1016/j.vetpar.2011.08.010
31 https://doi.org/10.1016/j.vetpar.2012.10.013
32 https://doi.org/10.1016/s0304-4017(03)00061-x
33 https://doi.org/10.1016/s0304-4017(97)00208-2
34 https://doi.org/10.1086/383281
35 https://doi.org/10.1093/jac/dkr185
36 https://doi.org/10.1128/cdli.9.5.1137-1141.2002
37 https://doi.org/10.1128/jcm.00558-12
38 https://doi.org/10.1128/jcm.39.2.613-617.2001
39 https://doi.org/10.1128/jcm.40.1.210-215.2002
40 https://doi.org/10.1128/jcm.42.11.5249-5255.2004
41 https://doi.org/10.1371/journal.pntd.0001596
42 https://doi.org/10.1371/journal.pntd.0001955
43 https://doi.org/10.1371/journal.pone.0035671
44 https://doi.org/10.1371/journal.pone.0088845
45 https://doi.org/10.1371/journal.pone.0091009
46 https://doi.org/10.1371/journal.pone.0103635
47 https://doi.org/10.4269/ajtmh.1996.55.273
48 https://doi.org/10.4269/ajtmh.2006.75.858
49 https://doi.org/10.4269/ajtmh.2010.09-0366
50 schema:datePublished 2014-12
51 schema:datePublishedReg 2014-12-01
52 schema:description BACKGROUND: In leishmaniasis caused by Leishmania infantum, the dog acts as the main reservoir for the disease. Non-invasive sampling for Leishmania detection is pivotal for rapid and affordable diagnosis. Recently, the use of conjunctival swab (CS) has been evaluated as a non-invasive sampling technique for quantitative real-time PCR (qPCR). However, few investigations have been made on the applicability of CS qPCR in particular cases such as dogs with borderline IFAT titres, suspected disease relapse with comorbidity and therapy monitoring. The aims of this study were i) to confirm the efficacy of CS, comparing these samples to buffy coat (BC) samples, as effective non-invasive samples for Leishmania quantitative detection by qPCR and ii) to verify the usefulness of qPCR compared to conventional laboratory and clinical parameters to assist in therapeutic decision making regarding dogs with complex clinical cases. METHODS: Eighty dogs were divided into 4 groups based on their IFAT titres and clinical histories. Two qPCR assays were performed both on CS raw lysates and on purified DNA from BC samples. The assays were then compared. Z tests for difference of proportion, with Bonferroni correction, were carried out to evaluate the qPCR results. Logistic regression with backward stepwise elimination was performed to detect the subset of haematochemical variables significantly associated with PCR positivity. RESULTS: The qPCR performed on CS samples showed better sensitivity (87%) and specificity (96%) than assays carried out using BC samples, regardless of the primers used. The haematochemical parameters haemoglobin and globulins were found to be significantly associated with qPCR positivity. Pearson correlations between Leishmania kDNA load in CS and body condition scores or IFAT titres were calculated in dogs with new leishmaniasis diagnoses. The Leishmania kDNA load in CS correlated moderately with IFAT titres (R = 0.59) but a very weak correlation (R = 0.37) with body condition score (BCS) was found. CONCLUSIONS: The applicability of CS for Leishmania detection in dogs was confirmed, revealing the usefulness of raw lysates for quantitative purposes. Moreover, the qPCR was found to be particularly useful in cases lacking a clear clinical diagnosis, where the haematochemical values cannot be predictive.
53 schema:genre research_article
54 schema:inLanguage en
55 schema:isAccessibleForFree true
56 schema:isPartOf N44f1452b9d734b519d9ba7f301dbc3d8
57 N50b77716a6504a76994c41bf917cb81f
58 sg:journal.1039458
59 schema:name Application of qPCR in conjunctival swab samples for the evaluation of canine leishmaniasis in borderline cases or disease relapse and correlation with clinical parameters
60 schema:pagination 460
61 schema:productId N7c52e46f261a435ea79211023d1a5a9d
62 N9b15eefb05fc40d397ccff3519249ddb
63 Naef0a158e959494c8513ac8ed50bb441
64 Nf3009ad8c928409b881907a879b4747e
65 Nf708fa76aadd4ceaab878cb2ecf3a853
66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053214130
67 https://doi.org/10.1186/s13071-014-0460-3
68 schema:sdDatePublished 2019-04-11T13:10
69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
70 schema:sdPublisher Nb7cb10063c394430a85f97400ddd3b31
71 schema:url http://link.springer.com/10.1186%2Fs13071-014-0460-3
72 sgo:license sg:explorer/license/
73 sgo:sdDataset articles
74 rdf:type schema:ScholarlyArticle
75 N00dd7e8e86974d16aaf233366a84788e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Recurrence
77 rdf:type schema:DefinedTerm
78 N14fef08003444781a81f732b899de329 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Animals
80 rdf:type schema:DefinedTerm
81 N22885372a82f4a96a6397155593af5d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Polymerase Chain Reaction
83 rdf:type schema:DefinedTerm
84 N3122bdd332ee4ae7932270ccd1670532 schema:name Veterinary Clinic “Santa Teresa”, via Piave 23, 61032, Fano, PU, Italy
85 rdf:type schema:Organization
86 N3b114752dc4b4f2397d04e06d7267ef0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Dog Diseases
88 rdf:type schema:DefinedTerm
89 N44f1452b9d734b519d9ba7f301dbc3d8 schema:volumeNumber 7
90 rdf:type schema:PublicationVolume
91 N50b77716a6504a76994c41bf917cb81f schema:issueNumber 1
92 rdf:type schema:PublicationIssue
93 N5507cc537cdd4a778927f14bad72ffba schema:affiliation N3122bdd332ee4ae7932270ccd1670532
94 schema:familyName Bianchi
95 schema:givenName Barbara
96 rdf:type schema:Person
97 N5e739e414ebf47e690ac07b06c9019b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Antiprotozoal Agents
99 rdf:type schema:DefinedTerm
100 N7c52e46f261a435ea79211023d1a5a9d schema:name nlm_unique_id
101 schema:value 101462774
102 rdf:type schema:PropertyValue
103 N7db2c567ff174a23ac2e6995db705f72 rdf:first N5507cc537cdd4a778927f14bad72ffba
104 rdf:rest N7edb865e8707405fb5b488bb42db43ab
105 N7edb865e8707405fb5b488bb42db43ab rdf:first sg:person.0752252542.98
106 rdf:rest rdf:nil
107 N82bdd5e901bc4c3dacee632a770e0665 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Dogs
109 rdf:type schema:DefinedTerm
110 N9b15eefb05fc40d397ccff3519249ddb schema:name dimensions_id
111 schema:value pub.1053214130
112 rdf:type schema:PropertyValue
113 Na560a7ea18ec4515af68ae1053c151e8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Female
115 rdf:type schema:DefinedTerm
116 Nabcee16b8fc949d8bebaa9b73f63c701 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Male
118 rdf:type schema:DefinedTerm
119 Naef0a158e959494c8513ac8ed50bb441 schema:name readcube_id
120 schema:value fd7879920ed9af88b881099b0c827b3ae86d6fe502525c3a4b1181092c45dad6
121 rdf:type schema:PropertyValue
122 Nb462d52003d043759bf2ad8fb111051b rdf:first sg:person.01101522732.44
123 rdf:rest Ne3b88ee9aa914bfd8233c80479d80d3e
124 Nb7cb10063c394430a85f97400ddd3b31 schema:name Springer Nature - SN SciGraph project
125 rdf:type schema:Organization
126 Nb7f28eb8cfd14ce4bd2c9e5eedba2275 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Leishmaniasis, Visceral
128 rdf:type schema:DefinedTerm
129 Nbf2fda3879b44a658a18a7b51267a4da rdf:first sg:person.01331435666.80
130 rdf:rest N7db2c567ff174a23ac2e6995db705f72
131 Nc2df932c6c174444b7f0dc1fb70dffdf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Conjunctiva
133 rdf:type schema:DefinedTerm
134 Ne3b88ee9aa914bfd8233c80479d80d3e rdf:first sg:person.0617701361.11
135 rdf:rest Nbf2fda3879b44a658a18a7b51267a4da
136 Nf3009ad8c928409b881907a879b4747e schema:name doi
137 schema:value 10.1186/s13071-014-0460-3
138 rdf:type schema:PropertyValue
139 Nf708fa76aadd4ceaab878cb2ecf3a853 schema:name pubmed_id
140 schema:value 25331737
141 rdf:type schema:PropertyValue
142 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
143 schema:name Medical and Health Sciences
144 rdf:type schema:DefinedTerm
145 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
146 schema:name Clinical Sciences
147 rdf:type schema:DefinedTerm
148 sg:journal.1039458 schema:issn 1756-3305
149 schema:name Filaria Journal
150 rdf:type schema:Periodical
151 sg:person.01101522732.44 schema:affiliation https://www.grid.ac/institutes/grid.12711.34
152 schema:familyName Ceccarelli
153 schema:givenName Marcello
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101522732.44
155 rdf:type schema:Person
156 sg:person.01331435666.80 schema:affiliation https://www.grid.ac/institutes/grid.12711.34
157 schema:familyName Sisti
158 schema:givenName Davide
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01331435666.80
160 rdf:type schema:Person
161 sg:person.0617701361.11 schema:affiliation https://www.grid.ac/institutes/grid.12711.34
162 schema:familyName Galluzzi
163 schema:givenName Luca
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617701361.11
165 rdf:type schema:Person
166 sg:person.0752252542.98 schema:affiliation https://www.grid.ac/institutes/grid.12711.34
167 schema:familyName Magnani
168 schema:givenName Mauro
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752252542.98
170 rdf:type schema:Person
171 sg:pub.10.1007/bf00937115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082500267
172 https://doi.org/10.1007/bf00937115
173 rdf:type schema:CreativeWork
174 sg:pub.10.1023/a:1007526401175 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009777883
175 https://doi.org/10.1023/a:1007526401175
176 rdf:type schema:CreativeWork
177 sg:pub.10.1186/1756-3305-4-86 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004739227
178 https://doi.org/10.1186/1756-3305-4-86
179 rdf:type schema:CreativeWork
180 sg:pub.10.1186/1756-3305-5-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037470936
181 https://doi.org/10.1186/1756-3305-5-15
182 rdf:type schema:CreativeWork
183 https://app.dimensions.ai/details/publication/pub.1074507808 schema:CreativeWork
184 https://doi.org/10.1016/0035-9203(92)90295-n schema:sameAs https://app.dimensions.ai/details/publication/pub.1037529058
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1016/j.biopsych.2005.09.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026396817
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1016/j.jcpa.2007.09.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009764493
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/j.micinf.2007.06.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047612873
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/j.rvsc.2009.02.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036576844
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/j.rvsc.2011.01.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022257053
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/j.tvjl.2007.04.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049931092
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/j.tvjl.2013.07.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041981153
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/j.vetpar.2008.07.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046598503
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1016/j.vetpar.2009.05.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046148433
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/j.vetpar.2010.03.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026119281
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/j.vetpar.2011.08.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014283057
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/j.vetpar.2012.10.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029856185
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/s0304-4017(03)00061-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043119459
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/s0304-4017(97)00208-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017215367
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1086/383281 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027085287
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1093/jac/dkr185 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014130960
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1128/cdli.9.5.1137-1141.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014499409
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1128/jcm.00558-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036249656
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1128/jcm.39.2.613-617.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007412134
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1128/jcm.40.1.210-215.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012163138
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1128/jcm.42.11.5249-5255.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050729371
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1371/journal.pntd.0001596 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012642431
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1371/journal.pntd.0001955 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002506093
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1371/journal.pone.0035671 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033992319
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1371/journal.pone.0088845 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000320145
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1371/journal.pone.0091009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022084754
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1371/journal.pone.0103635 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020871602
239 rdf:type schema:CreativeWork
240 https://doi.org/10.4269/ajtmh.1996.55.273 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082972767
241 rdf:type schema:CreativeWork
242 https://doi.org/10.4269/ajtmh.2006.75.858 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077327855
243 rdf:type schema:CreativeWork
244 https://doi.org/10.4269/ajtmh.2010.09-0366 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029306635
245 rdf:type schema:CreativeWork
246 https://www.grid.ac/institutes/grid.12711.34 schema:alternateName University of Urbino
247 schema:name Department of Biomolecular Sciences, University of Urbino “Carlo Bo”, via Saffi 2, 61029, Urbino, PU, Italy
248 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...