Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Xiaolu Li, Yucai He, Libing Zhang, Zhangyang Xu, Haoxi Ben, Matthew J. Gaffrey, Yongfu Yang, Shihui Yang, Joshua S. Yuan, Wei-Jun Qian, Bin Yang

ABSTRACT

Background: Biological routes for utilizing both carbohydrates and lignin are important to reach the ultimate goal of bioconversion of full carbon in biomass into biofuels and biochemicals. Recent biotechnology advances have shown promises toward facilitating biological transformation of lignin into lipids. Natural and engineered Rhodococcus strains (e.g., R. opacus PD630, R. jostii RHA1, and R. jostii RHA1 VanA-) have been demonstrated to utilize lignin for lipid production, and co-culture of them can promote lipid production from lignin. Results: In this study, a co-fermentation module of natural and engineered Rhodococcus strains with significant improved lignin degradation and/or lipid biosynthesis capacities was established, which enabled simultaneous conversion of glucose, lignin, and its derivatives into lipids. Although Rhodococci sp. showed preference to glucose over lignin, nearly half of the lignin was quickly depolymerized to monomers by these strains for cell growth and lipid synthesis after glucose was nearly consumed up. Profiles of metabolites produced by Rhodococcus strains growing on different carbon sources (e.g., glucose, alkali lignin, and dilute acid flowthrough-pretreated poplar wood slurry) confirmed lignin conversion during co-fermentation, and indicated novel metabolic capacities and unexplored metabolic pathways in these organisms. Proteome profiles suggested that lignin depolymerization by Rhodococci sp. involved multiple peroxidases with accessory oxidases. Besides the β-ketoadipate pathway, the phenylacetic acid (PAA) pathway was another potential route for the in vivo ring cleavage activity. In addition, deficiency of reducing power and cellular oxidative stress probably led to lower lipid production using lignin as the sole carbon source than that using glucose. Conclusions: This work demonstrated a potential strategy for efficient bioconversion of both lignin and glucose into lipids by co-culture of multiple natural and engineered Rhodococcus strains. In addition, the involvement of PAA pathway in lignin degradation can help to further improve lignin utilization, and the combinatory proteomics and bioinformatics strategies used in this study can also be applied into other systems to reveal the metabolic and regulatory pathways for balanced cellular metabolism and to select genetic targets for efficient conversion of both lignin and carbohydrates into biofuels. More... »

PAGES

60

References to SciGraph publications

  • 2014-10. Induction and carbon catabolite repression of phenol degradation genes in Rhodococcus erythropolis and Rhodococcus jostii in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2008-09. Structural basis of enzyme encapsulation into a bacterial nanocompartment in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2007-03. Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry in NATURE METHODS
  • 2013-09. Lignin: untapped biopolymers in biomass conversion technologies in BIOMASS CONVERSION AND BIOREFINERY
  • 2010. Biology of Rhodococcus in NONE
  • 2001-05. High cell density cultivation of Rhodococcus opacus for lipid production at a pilot-plant scale in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2015-12. Characterization of lignin derived from water-only and dilute acid flowthrough pretreatment of poplar wood at elevated temperatures in BIOTECHNOLOGY FOR BIOFUELS
  • 2014-12. MS-GF+ makes progress towards a universal database search tool for proteomics in NATURE COMMUNICATIONS
  • 2014-12. Enhancement of total sugar and lignin yields through dissolution of poplar wood by hot water and dilute acid flowthrough pretreatment in BIOTECHNOLOGY FOR BIOFUELS
  • 2012-01. Bioconversion of lignin model compounds with oleaginous Rhodococci in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2016-07. Characterization of key triacylglycerol biosynthesis processes in rhodococci in SCIENTIFIC REPORTS
  • 2013-12. Engineering xylose metabolism in triacylglycerol-producing Rhodococcus opacusfor lignocellulosic fuel production in BIOTECHNOLOGY FOR BIOFUELS
  • 2017-12. Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus in AMB EXPRESS
  • 2015-12. Designer synthetic media for studying microbial-catalyzed biofuel production in BIOTECHNOLOGY FOR BIOFUELS
  • 2008-12. Biosynthesis of storage compounds by Rhodococcus jostii RHA1 and global identification of genes involved in their metabolism in BMC GENOMICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13068-019-1395-x

    DOI

    http://dx.doi.org/10.1186/s13068-019-1395-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112876744

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30923568


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Washington State University", 
              "id": "https://www.grid.ac/institutes/grid.30064.31", 
              "name": [
                "Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, 2710 Crimson Way, 99354, Richland, WA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Xiaolu", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Washington State University", 
              "id": "https://www.grid.ac/institutes/grid.30064.31", 
              "name": [
                "Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, 2710 Crimson Way, 99354, Richland, WA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "He", 
            "givenName": "Yucai", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Washington State University", 
              "id": "https://www.grid.ac/institutes/grid.30064.31", 
              "name": [
                "Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, 2710 Crimson Way, 99354, Richland, WA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Libing", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Washington State University", 
              "id": "https://www.grid.ac/institutes/grid.30064.31", 
              "name": [
                "Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, 2710 Crimson Way, 99354, Richland, WA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xu", 
            "givenName": "Zhangyang", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Washington State University", 
              "id": "https://www.grid.ac/institutes/grid.30064.31", 
              "name": [
                "Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, 2710 Crimson Way, 99354, Richland, WA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ben", 
            "givenName": "Haoxi", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Pacific Northwest National Laboratory", 
              "id": "https://www.grid.ac/institutes/grid.451303.0", 
              "name": [
                "Biological Sciences Division, Pacific Northwest National Laboratory, 99352, Richland, WA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gaffrey", 
            "givenName": "Matthew J.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hubei University", 
              "id": "https://www.grid.ac/institutes/grid.34418.3a", 
              "name": [
                "State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei University, 430062, Wuhan, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yang", 
            "givenName": "Yongfu", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hubei University", 
              "id": "https://www.grid.ac/institutes/grid.34418.3a", 
              "name": [
                "State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei University, 430062, Wuhan, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yang", 
            "givenName": "Shihui", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Texas A&M University", 
              "id": "https://www.grid.ac/institutes/grid.264756.4", 
              "name": [
                "Department of Plant Pathology and Microbiology, Texas A&M University, 77840, College Station, TX, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yuan", 
            "givenName": "Joshua S.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Pacific Northwest National Laboratory", 
              "id": "https://www.grid.ac/institutes/grid.451303.0", 
              "name": [
                "Biological Sciences Division, Pacific Northwest National Laboratory, 99352, Richland, WA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Qian", 
            "givenName": "Wei-Jun", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Pacific Northwest National Laboratory", 
              "id": "https://www.grid.ac/institutes/grid.451303.0", 
              "name": [
                "Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, 2710 Crimson Way, 99354, Richland, WA, USA", 
                "Biological Sciences Division, Pacific Northwest National Laboratory, 99352, Richland, WA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yang", 
            "givenName": "Bin", 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1128/jb.01122-07", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000647102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1754-6834-6-134", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003271190", 
              "https://doi.org/10.1186/1754-6834-6-134"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.algal.2012.11.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003830628"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/c1np00042j", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003872213"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.micres.2009.11.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004112531"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bbb.1422", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005703083"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1410657111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005848879"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jbiotec.2016.08.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005978720"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biotechadv.2016.10.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007863920"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/febs.13224", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009194241"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth1019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009487848", 
              "https://doi.org/10.1038/nmeth1019"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13068-015-0377-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009517344", 
              "https://doi.org/10.1186/s13068-015-0377-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2164-9-600", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009630723", 
              "https://doi.org/10.1186/1471-2164-9-600"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cbpa.2015.10.018", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009846593"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cbpa.2015.10.018", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009846593"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms6277", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010070877", 
              "https://doi.org/10.1038/ncomms6277"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0607048103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010227561"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.186.15.5062-5077.2004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014327724"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jbiotec.2010.04.003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017837139"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/c5gc01955a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017933176"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/c5gc00422e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018057940"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0134752", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018296689"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.187.13.4497-4504.2005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019615033"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.micro.50.1.553", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022415050"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.1473", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022431961", 
              "https://doi.org/10.1038/nsmb.1473"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-014-5881-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024427611", 
              "https://doi.org/10.1007/s00253-014-5881-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/c5gc01165e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024913992"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.copbio.2016.02.030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025330486"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.copbio.2016.02.030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025330486"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.copbio.2016.02.030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025330486"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.copbio.2016.02.030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025330486"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.copbio.2016.02.030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025330486"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/lipi.19970990704", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027711938"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0378-1119(96)00748-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028705166"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-011-3743-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028778554", 
              "https://doi.org/10.1007/s00253-011-3743-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002530000576", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028924211", 
              "https://doi.org/10.1007/s002530000576"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jaap.2015.10.016", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029318069"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep24985", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032761077", 
              "https://doi.org/10.1038/srep24985"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ja203972q", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032863343"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ja203972q", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032863343"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1754-6834-7-76", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032917259", 
              "https://doi.org/10.1186/1754-6834-7-76"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/c4ee03230f", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032971596"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.183.24.7182-7189.2001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033933720"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.25702", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034505181"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pgen.1002219", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034574117"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbamem.2006.02.015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034888104"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/b908966g", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035013165"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/b908966g", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035013165"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s13399-013-0084-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035149590", 
              "https://doi.org/10.1007/s13399-013-0084-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13068-014-0179-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035594727", 
              "https://doi.org/10.1186/s13068-014-0179-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13068-014-0179-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035594727", 
              "https://doi.org/10.1186/s13068-014-0179-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1214/aos/1013699998", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036427477"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/aem.06876-11", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036598022"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.187.12.4050-4063.2005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039524787"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/c3gc42041h", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041334294"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-12937-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041900422", 
              "https://doi.org/10.1007/978-3-642-12937-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-12937-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041900422", 
              "https://doi.org/10.1007/978-3-642-12937-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biortech.2014.02.133", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042409326"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biortech.2016.10.059", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046080462"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biortech.2016.10.059", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046080462"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biortech.2016.10.059", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046080462"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/aem.69.11.6500-6506.2003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046241380"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/c6gc01131d", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047918353"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/c3gc40434j", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049858896"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.183.18.5426-5430.2001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051333022"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkt932", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053682623"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ac60252a045", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055054007"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/acschembio.5b00298", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055134966"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/acssuschemeng.6b02401", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055140655"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/acssuschemeng.6b02627", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055140695"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi101892z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055203865"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi101892z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055203865"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/cb400505a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055226508"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/pr050300l", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056290830"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/00221287-146-5-1143", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060370362"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/mic.0.000028", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060393943"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/mic.0.000424", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060394325"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1246843", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062469181"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.171.11.6251-6258.1989", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062717497"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077121078", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077121078", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1078353149", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082595945", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083265365", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083268378", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/c7gc00346c", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084967573"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/acssynbio.7b00192", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090907548"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13568-017-0484-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091995532", 
              "https://doi.org/10.1186/s13568-017-0484-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bbb.1825", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092164704"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biortech.2018.11.046", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1109812224"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biortech.2018.11.046", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1109812224"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biortech.2018.11.046", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1109812224"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.2517-6161.1995.tb02031.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1110458929"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.2517-6161.1995.tb02031.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1110458929"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.rser.2019.02.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1112193853"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.rser.2019.02.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1112193853"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.rser.2019.02.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1112193853"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.rser.2019.02.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1112193853"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2019-12", 
        "datePublishedReg": "2019-12-01", 
        "description": "Background: Biological routes for utilizing both carbohydrates and lignin are important to reach the ultimate goal of bioconversion of full carbon in biomass into biofuels and biochemicals.\u00a0Recent biotechnology advances have shown promises toward facilitating biological transformation of lignin into lipids. Natural and engineered\u00a0Rhodococcus strains\u00a0(e.g.,\u00a0R. opacus PD630, R. jostii\u00a0RHA1, and R. jostii\u00a0RHA1 VanA-) have been demonstrated to utilize lignin for lipid production, and co-culture of them can promote lipid production from lignin.\nResults: In this study, a co-fermentation module of natural and engineered\u00a0Rhodococcus strains\u00a0with significant improved lignin degradation and/or lipid biosynthesis capacities was established, which enabled simultaneous conversion of glucose, lignin, and its derivatives into lipids. Although\u00a0Rhodococci\u00a0sp. showed preference to glucose over lignin, nearly half of the lignin was quickly depolymerized to monomers by these strains for cell growth and lipid synthesis after glucose was nearly consumed up. Profiles of metabolites produced by Rhodococcus\u00a0strains\u00a0growing on different carbon sources (e.g., glucose, alkali lignin, and dilute acid flowthrough-pretreated poplar wood slurry)\u00a0confirmed lignin conversion during co-fermentation, and indicated novel metabolic capacities and unexplored metabolic pathways in these organisms.\u00a0Proteome profiles suggested that lignin depolymerization by\u00a0Rhodococci sp. involved multiple peroxidases with accessory oxidases. Besides the \u03b2-ketoadipate pathway, the\u00a0phenylacetic acid (PAA) pathway was another potential route for the\u00a0in vivo\u00a0ring cleavage activity. In addition,\u00a0deficiency of reducing power and cellular oxidative stress probably led to lower lipid production using lignin as the sole carbon source than that using glucose.\nConclusions: This work demonstrated a potential strategy for efficient bioconversion of both lignin and glucose into lipids by co-culture of multiple natural and engineered Rhodococcus strains. In addition, the involvement of PAA pathway in lignin degradation can help to further improve lignin utilization, and the combinatory proteomics and bioinformatics strategies used in this study can also be applied into other systems to reveal the metabolic and regulatory pathways for balanced cellular metabolism and to select genetic targets for efficient conversion\u00a0of both lignin and carbohydrates into biofuels.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1186/s13068-019-1395-x", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1039046", 
            "issn": [
              "1754-6834"
            ], 
            "name": "Biotechnology for Biofuels", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "12"
          }
        ], 
        "name": "Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains", 
        "pagination": "60", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "8142040e80ec7b2679b585c49968b35992820e1f33402568547f2450c60dfa01"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30923568"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101316935"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/s13068-019-1395-x"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1112876744"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/s13068-019-1395-x", 
          "https://app.dimensions.ai/details/publication/pub.1112876744"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:48", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130792_00000006.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://link.springer.com/10.1186%2Fs13068-019-1395-x"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13068-019-1395-x'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13068-019-1395-x'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13068-019-1395-x'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13068-019-1395-x'


     

    This table displays all metadata directly associated to this object as RDF triples.

    384 TRIPLES      21 PREDICATES      108 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/s13068-019-1395-x schema:about anzsrc-for:06
    2 anzsrc-for:0601
    3 schema:author N06c480e11d0943169c54a7b6ca57754a
    4 schema:citation sg:pub.10.1007/978-3-642-12937-7
    5 sg:pub.10.1007/s00253-011-3743-z
    6 sg:pub.10.1007/s00253-014-5881-6
    7 sg:pub.10.1007/s002530000576
    8 sg:pub.10.1007/s13399-013-0084-4
    9 sg:pub.10.1038/ncomms6277
    10 sg:pub.10.1038/nmeth1019
    11 sg:pub.10.1038/nsmb.1473
    12 sg:pub.10.1038/srep24985
    13 sg:pub.10.1186/1471-2164-9-600
    14 sg:pub.10.1186/1754-6834-6-134
    15 sg:pub.10.1186/1754-6834-7-76
    16 sg:pub.10.1186/s13068-014-0179-6
    17 sg:pub.10.1186/s13068-015-0377-x
    18 sg:pub.10.1186/s13568-017-0484-0
    19 https://app.dimensions.ai/details/publication/pub.1077121078
    20 https://app.dimensions.ai/details/publication/pub.1078353149
    21 https://app.dimensions.ai/details/publication/pub.1082595945
    22 https://app.dimensions.ai/details/publication/pub.1083265365
    23 https://app.dimensions.ai/details/publication/pub.1083268378
    24 https://doi.org/10.1002/bbb.1422
    25 https://doi.org/10.1002/bbb.1825
    26 https://doi.org/10.1002/bit.25702
    27 https://doi.org/10.1002/lipi.19970990704
    28 https://doi.org/10.1016/j.algal.2012.11.004
    29 https://doi.org/10.1016/j.bbamem.2006.02.015
    30 https://doi.org/10.1016/j.biortech.2014.02.133
    31 https://doi.org/10.1016/j.biortech.2016.10.059
    32 https://doi.org/10.1016/j.biortech.2018.11.046
    33 https://doi.org/10.1016/j.biotechadv.2016.10.001
    34 https://doi.org/10.1016/j.cbpa.2015.10.018
    35 https://doi.org/10.1016/j.copbio.2016.02.030
    36 https://doi.org/10.1016/j.jaap.2015.10.016
    37 https://doi.org/10.1016/j.jbiotec.2010.04.003
    38 https://doi.org/10.1016/j.jbiotec.2016.08.011
    39 https://doi.org/10.1016/j.micres.2009.11.011
    40 https://doi.org/10.1016/j.rser.2019.02.009
    41 https://doi.org/10.1016/s0378-1119(96)00748-2
    42 https://doi.org/10.1021/ac60252a045
    43 https://doi.org/10.1021/acschembio.5b00298
    44 https://doi.org/10.1021/acssuschemeng.6b02401
    45 https://doi.org/10.1021/acssuschemeng.6b02627
    46 https://doi.org/10.1021/acssynbio.7b00192
    47 https://doi.org/10.1021/bi101892z
    48 https://doi.org/10.1021/cb400505a
    49 https://doi.org/10.1021/ja203972q
    50 https://doi.org/10.1021/pr050300l
    51 https://doi.org/10.1039/b908966g
    52 https://doi.org/10.1039/c1np00042j
    53 https://doi.org/10.1039/c3gc40434j
    54 https://doi.org/10.1039/c3gc42041h
    55 https://doi.org/10.1039/c4ee03230f
    56 https://doi.org/10.1039/c5gc00422e
    57 https://doi.org/10.1039/c5gc01165e
    58 https://doi.org/10.1039/c5gc01955a
    59 https://doi.org/10.1039/c6gc01131d
    60 https://doi.org/10.1039/c7gc00346c
    61 https://doi.org/10.1073/pnas.0607048103
    62 https://doi.org/10.1073/pnas.1410657111
    63 https://doi.org/10.1093/nar/gkt932
    64 https://doi.org/10.1099/00221287-146-5-1143
    65 https://doi.org/10.1099/mic.0.000028
    66 https://doi.org/10.1099/mic.0.000424
    67 https://doi.org/10.1111/febs.13224
    68 https://doi.org/10.1111/j.2517-6161.1995.tb02031.x
    69 https://doi.org/10.1126/science.1246843
    70 https://doi.org/10.1128/aem.06876-11
    71 https://doi.org/10.1128/aem.69.11.6500-6506.2003
    72 https://doi.org/10.1128/jb.01122-07
    73 https://doi.org/10.1128/jb.171.11.6251-6258.1989
    74 https://doi.org/10.1128/jb.183.18.5426-5430.2001
    75 https://doi.org/10.1128/jb.183.24.7182-7189.2001
    76 https://doi.org/10.1128/jb.186.15.5062-5077.2004
    77 https://doi.org/10.1128/jb.187.12.4050-4063.2005
    78 https://doi.org/10.1128/jb.187.13.4497-4504.2005
    79 https://doi.org/10.1146/annurev.micro.50.1.553
    80 https://doi.org/10.1214/aos/1013699998
    81 https://doi.org/10.1371/journal.pgen.1002219
    82 https://doi.org/10.1371/journal.pone.0134752
    83 schema:datePublished 2019-12
    84 schema:datePublishedReg 2019-12-01
    85 schema:description Background: Biological routes for utilizing both carbohydrates and lignin are important to reach the ultimate goal of bioconversion of full carbon in biomass into biofuels and biochemicals. Recent biotechnology advances have shown promises toward facilitating biological transformation of lignin into lipids. Natural and engineered Rhodococcus strains (e.g., R. opacus PD630, R. jostii RHA1, and R. jostii RHA1 VanA-) have been demonstrated to utilize lignin for lipid production, and co-culture of them can promote lipid production from lignin. Results: In this study, a co-fermentation module of natural and engineered Rhodococcus strains with significant improved lignin degradation and/or lipid biosynthesis capacities was established, which enabled simultaneous conversion of glucose, lignin, and its derivatives into lipids. Although Rhodococci sp. showed preference to glucose over lignin, nearly half of the lignin was quickly depolymerized to monomers by these strains for cell growth and lipid synthesis after glucose was nearly consumed up. Profiles of metabolites produced by Rhodococcus strains growing on different carbon sources (e.g., glucose, alkali lignin, and dilute acid flowthrough-pretreated poplar wood slurry) confirmed lignin conversion during co-fermentation, and indicated novel metabolic capacities and unexplored metabolic pathways in these organisms. Proteome profiles suggested that lignin depolymerization by Rhodococci sp. involved multiple peroxidases with accessory oxidases. Besides the β-ketoadipate pathway, the phenylacetic acid (PAA) pathway was another potential route for the in vivo ring cleavage activity. In addition, deficiency of reducing power and cellular oxidative stress probably led to lower lipid production using lignin as the sole carbon source than that using glucose. Conclusions: This work demonstrated a potential strategy for efficient bioconversion of both lignin and glucose into lipids by co-culture of multiple natural and engineered Rhodococcus strains. In addition, the involvement of PAA pathway in lignin degradation can help to further improve lignin utilization, and the combinatory proteomics and bioinformatics strategies used in this study can also be applied into other systems to reveal the metabolic and regulatory pathways for balanced cellular metabolism and to select genetic targets for efficient conversion of both lignin and carbohydrates into biofuels.
    86 schema:genre research_article
    87 schema:inLanguage en
    88 schema:isAccessibleForFree true
    89 schema:isPartOf N8d7ec962442f46518977cebdc069c372
    90 Na09ad6ce98974498825a527bc525e714
    91 sg:journal.1039046
    92 schema:name Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains
    93 schema:pagination 60
    94 schema:productId N2bc8f5997b304199b0eb7fd0f7114935
    95 N6e26acbcf6fb4a0f99b748a5980ef52f
    96 N82da9583131c4212a6816e18d82168a2
    97 Na0b13115e87644dfbe6940df30d06607
    98 Ndb3516e1f57247559b436d8621a5ac49
    99 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112876744
    100 https://doi.org/10.1186/s13068-019-1395-x
    101 schema:sdDatePublished 2019-04-11T13:48
    102 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    103 schema:sdPublisher Nd5772dd131eb4469aa96e9e1ef2c47ae
    104 schema:url https://link.springer.com/10.1186%2Fs13068-019-1395-x
    105 sgo:license sg:explorer/license/
    106 sgo:sdDataset articles
    107 rdf:type schema:ScholarlyArticle
    108 N06c480e11d0943169c54a7b6ca57754a rdf:first N473c8bec349f41c5b1d66baddc16b468
    109 rdf:rest N335cd92cdecc4d82a0d6149c50b35f56
    110 N0e7d381dfe3c47e38b5e05ba7f1383c5 schema:affiliation https://www.grid.ac/institutes/grid.451303.0
    111 schema:familyName Yang
    112 schema:givenName Bin
    113 rdf:type schema:Person
    114 N12341e78d51d436cb9fa2e4caff83e6d schema:affiliation https://www.grid.ac/institutes/grid.451303.0
    115 schema:familyName Gaffrey
    116 schema:givenName Matthew J.
    117 rdf:type schema:Person
    118 N1cf96f3d66c04c0c8176387bf079f458 schema:affiliation https://www.grid.ac/institutes/grid.264756.4
    119 schema:familyName Yuan
    120 schema:givenName Joshua S.
    121 rdf:type schema:Person
    122 N2bc8f5997b304199b0eb7fd0f7114935 schema:name readcube_id
    123 schema:value 8142040e80ec7b2679b585c49968b35992820e1f33402568547f2450c60dfa01
    124 rdf:type schema:PropertyValue
    125 N335cd92cdecc4d82a0d6149c50b35f56 rdf:first N505440e45efb404eb300959e51a6c628
    126 rdf:rest N45617da1c9354120b5b285072e9d9cb5
    127 N45617da1c9354120b5b285072e9d9cb5 rdf:first N8742483d03144001a0fbc5975697ece3
    128 rdf:rest N9da8655852bb4f6694414805d8aec452
    129 N473c8bec349f41c5b1d66baddc16b468 schema:affiliation https://www.grid.ac/institutes/grid.30064.31
    130 schema:familyName Li
    131 schema:givenName Xiaolu
    132 rdf:type schema:Person
    133 N49728480a04e4c679ebdf51dcddc3ae5 schema:affiliation https://www.grid.ac/institutes/grid.30064.31
    134 schema:familyName Xu
    135 schema:givenName Zhangyang
    136 rdf:type schema:Person
    137 N4c24d7dba6104ce3995c9a430e403c91 rdf:first N1cf96f3d66c04c0c8176387bf079f458
    138 rdf:rest N99ce52f20a7647eaae3b9416713aca70
    139 N505440e45efb404eb300959e51a6c628 schema:affiliation https://www.grid.ac/institutes/grid.30064.31
    140 schema:familyName He
    141 schema:givenName Yucai
    142 rdf:type schema:Person
    143 N57a15491b77b4c7cab9bbdc1f672ad2a schema:affiliation https://www.grid.ac/institutes/grid.451303.0
    144 schema:familyName Qian
    145 schema:givenName Wei-Jun
    146 rdf:type schema:Person
    147 N631b2ea474e64af1a29a4c24808c619c schema:affiliation https://www.grid.ac/institutes/grid.30064.31
    148 schema:familyName Ben
    149 schema:givenName Haoxi
    150 rdf:type schema:Person
    151 N6e26acbcf6fb4a0f99b748a5980ef52f schema:name dimensions_id
    152 schema:value pub.1112876744
    153 rdf:type schema:PropertyValue
    154 N82da9583131c4212a6816e18d82168a2 schema:name doi
    155 schema:value 10.1186/s13068-019-1395-x
    156 rdf:type schema:PropertyValue
    157 N8742483d03144001a0fbc5975697ece3 schema:affiliation https://www.grid.ac/institutes/grid.30064.31
    158 schema:familyName Zhang
    159 schema:givenName Libing
    160 rdf:type schema:Person
    161 N8d7ec962442f46518977cebdc069c372 schema:volumeNumber 12
    162 rdf:type schema:PublicationVolume
    163 N99ce52f20a7647eaae3b9416713aca70 rdf:first N57a15491b77b4c7cab9bbdc1f672ad2a
    164 rdf:rest Nf0d02ecdf3c34d179e2131c9b1c1c6b3
    165 N9da8655852bb4f6694414805d8aec452 rdf:first N49728480a04e4c679ebdf51dcddc3ae5
    166 rdf:rest Nb1dce194d7ea4181a867d8c010bc5494
    167 N9e473f47dbd3418a913935150fcba151 rdf:first N12341e78d51d436cb9fa2e4caff83e6d
    168 rdf:rest Necedd6fd93604d94a507749e61664327
    169 N9f71771539564196aa4445cdc3869c53 schema:affiliation https://www.grid.ac/institutes/grid.34418.3a
    170 schema:familyName Yang
    171 schema:givenName Shihui
    172 rdf:type schema:Person
    173 Na09ad6ce98974498825a527bc525e714 schema:issueNumber 1
    174 rdf:type schema:PublicationIssue
    175 Na0b13115e87644dfbe6940df30d06607 schema:name pubmed_id
    176 schema:value 30923568
    177 rdf:type schema:PropertyValue
    178 Na4769289773f4cdab8e70498afe605b8 rdf:first N9f71771539564196aa4445cdc3869c53
    179 rdf:rest N4c24d7dba6104ce3995c9a430e403c91
    180 Nb1dce194d7ea4181a867d8c010bc5494 rdf:first N631b2ea474e64af1a29a4c24808c619c
    181 rdf:rest N9e473f47dbd3418a913935150fcba151
    182 Nd5772dd131eb4469aa96e9e1ef2c47ae schema:name Springer Nature - SN SciGraph project
    183 rdf:type schema:Organization
    184 Ndb3516e1f57247559b436d8621a5ac49 schema:name nlm_unique_id
    185 schema:value 101316935
    186 rdf:type schema:PropertyValue
    187 Ndf532a6042b344c28e59fce49e6a4925 schema:affiliation https://www.grid.ac/institutes/grid.34418.3a
    188 schema:familyName Yang
    189 schema:givenName Yongfu
    190 rdf:type schema:Person
    191 Necedd6fd93604d94a507749e61664327 rdf:first Ndf532a6042b344c28e59fce49e6a4925
    192 rdf:rest Na4769289773f4cdab8e70498afe605b8
    193 Nf0d02ecdf3c34d179e2131c9b1c1c6b3 rdf:first N0e7d381dfe3c47e38b5e05ba7f1383c5
    194 rdf:rest rdf:nil
    195 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    196 schema:name Biological Sciences
    197 rdf:type schema:DefinedTerm
    198 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    199 schema:name Biochemistry and Cell Biology
    200 rdf:type schema:DefinedTerm
    201 sg:journal.1039046 schema:issn 1754-6834
    202 schema:name Biotechnology for Biofuels
    203 rdf:type schema:Periodical
    204 sg:pub.10.1007/978-3-642-12937-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041900422
    205 https://doi.org/10.1007/978-3-642-12937-7
    206 rdf:type schema:CreativeWork
    207 sg:pub.10.1007/s00253-011-3743-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1028778554
    208 https://doi.org/10.1007/s00253-011-3743-z
    209 rdf:type schema:CreativeWork
    210 sg:pub.10.1007/s00253-014-5881-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024427611
    211 https://doi.org/10.1007/s00253-014-5881-6
    212 rdf:type schema:CreativeWork
    213 sg:pub.10.1007/s002530000576 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028924211
    214 https://doi.org/10.1007/s002530000576
    215 rdf:type schema:CreativeWork
    216 sg:pub.10.1007/s13399-013-0084-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035149590
    217 https://doi.org/10.1007/s13399-013-0084-4
    218 rdf:type schema:CreativeWork
    219 sg:pub.10.1038/ncomms6277 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010070877
    220 https://doi.org/10.1038/ncomms6277
    221 rdf:type schema:CreativeWork
    222 sg:pub.10.1038/nmeth1019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009487848
    223 https://doi.org/10.1038/nmeth1019
    224 rdf:type schema:CreativeWork
    225 sg:pub.10.1038/nsmb.1473 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022431961
    226 https://doi.org/10.1038/nsmb.1473
    227 rdf:type schema:CreativeWork
    228 sg:pub.10.1038/srep24985 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032761077
    229 https://doi.org/10.1038/srep24985
    230 rdf:type schema:CreativeWork
    231 sg:pub.10.1186/1471-2164-9-600 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009630723
    232 https://doi.org/10.1186/1471-2164-9-600
    233 rdf:type schema:CreativeWork
    234 sg:pub.10.1186/1754-6834-6-134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003271190
    235 https://doi.org/10.1186/1754-6834-6-134
    236 rdf:type schema:CreativeWork
    237 sg:pub.10.1186/1754-6834-7-76 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032917259
    238 https://doi.org/10.1186/1754-6834-7-76
    239 rdf:type schema:CreativeWork
    240 sg:pub.10.1186/s13068-014-0179-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035594727
    241 https://doi.org/10.1186/s13068-014-0179-6
    242 rdf:type schema:CreativeWork
    243 sg:pub.10.1186/s13068-015-0377-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009517344
    244 https://doi.org/10.1186/s13068-015-0377-x
    245 rdf:type schema:CreativeWork
    246 sg:pub.10.1186/s13568-017-0484-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091995532
    247 https://doi.org/10.1186/s13568-017-0484-0
    248 rdf:type schema:CreativeWork
    249 https://app.dimensions.ai/details/publication/pub.1077121078 schema:CreativeWork
    250 https://app.dimensions.ai/details/publication/pub.1078353149 schema:CreativeWork
    251 https://app.dimensions.ai/details/publication/pub.1082595945 schema:CreativeWork
    252 https://app.dimensions.ai/details/publication/pub.1083265365 schema:CreativeWork
    253 https://app.dimensions.ai/details/publication/pub.1083268378 schema:CreativeWork
    254 https://doi.org/10.1002/bbb.1422 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005703083
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1002/bbb.1825 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092164704
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1002/bit.25702 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034505181
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1002/lipi.19970990704 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027711938
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1016/j.algal.2012.11.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003830628
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1016/j.bbamem.2006.02.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034888104
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1016/j.biortech.2014.02.133 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042409326
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1016/j.biortech.2016.10.059 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046080462
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1016/j.biortech.2018.11.046 schema:sameAs https://app.dimensions.ai/details/publication/pub.1109812224
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1016/j.biotechadv.2016.10.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007863920
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1016/j.cbpa.2015.10.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009846593
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1016/j.copbio.2016.02.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025330486
    277 rdf:type schema:CreativeWork
    278 https://doi.org/10.1016/j.jaap.2015.10.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029318069
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1016/j.jbiotec.2010.04.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017837139
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1016/j.jbiotec.2016.08.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005978720
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1016/j.micres.2009.11.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004112531
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1016/j.rser.2019.02.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112193853
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1016/s0378-1119(96)00748-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028705166
    289 rdf:type schema:CreativeWork
    290 https://doi.org/10.1021/ac60252a045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055054007
    291 rdf:type schema:CreativeWork
    292 https://doi.org/10.1021/acschembio.5b00298 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055134966
    293 rdf:type schema:CreativeWork
    294 https://doi.org/10.1021/acssuschemeng.6b02401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055140655
    295 rdf:type schema:CreativeWork
    296 https://doi.org/10.1021/acssuschemeng.6b02627 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055140695
    297 rdf:type schema:CreativeWork
    298 https://doi.org/10.1021/acssynbio.7b00192 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090907548
    299 rdf:type schema:CreativeWork
    300 https://doi.org/10.1021/bi101892z schema:sameAs https://app.dimensions.ai/details/publication/pub.1055203865
    301 rdf:type schema:CreativeWork
    302 https://doi.org/10.1021/cb400505a schema:sameAs https://app.dimensions.ai/details/publication/pub.1055226508
    303 rdf:type schema:CreativeWork
    304 https://doi.org/10.1021/ja203972q schema:sameAs https://app.dimensions.ai/details/publication/pub.1032863343
    305 rdf:type schema:CreativeWork
    306 https://doi.org/10.1021/pr050300l schema:sameAs https://app.dimensions.ai/details/publication/pub.1056290830
    307 rdf:type schema:CreativeWork
    308 https://doi.org/10.1039/b908966g schema:sameAs https://app.dimensions.ai/details/publication/pub.1035013165
    309 rdf:type schema:CreativeWork
    310 https://doi.org/10.1039/c1np00042j schema:sameAs https://app.dimensions.ai/details/publication/pub.1003872213
    311 rdf:type schema:CreativeWork
    312 https://doi.org/10.1039/c3gc40434j schema:sameAs https://app.dimensions.ai/details/publication/pub.1049858896
    313 rdf:type schema:CreativeWork
    314 https://doi.org/10.1039/c3gc42041h schema:sameAs https://app.dimensions.ai/details/publication/pub.1041334294
    315 rdf:type schema:CreativeWork
    316 https://doi.org/10.1039/c4ee03230f schema:sameAs https://app.dimensions.ai/details/publication/pub.1032971596
    317 rdf:type schema:CreativeWork
    318 https://doi.org/10.1039/c5gc00422e schema:sameAs https://app.dimensions.ai/details/publication/pub.1018057940
    319 rdf:type schema:CreativeWork
    320 https://doi.org/10.1039/c5gc01165e schema:sameAs https://app.dimensions.ai/details/publication/pub.1024913992
    321 rdf:type schema:CreativeWork
    322 https://doi.org/10.1039/c5gc01955a schema:sameAs https://app.dimensions.ai/details/publication/pub.1017933176
    323 rdf:type schema:CreativeWork
    324 https://doi.org/10.1039/c6gc01131d schema:sameAs https://app.dimensions.ai/details/publication/pub.1047918353
    325 rdf:type schema:CreativeWork
    326 https://doi.org/10.1039/c7gc00346c schema:sameAs https://app.dimensions.ai/details/publication/pub.1084967573
    327 rdf:type schema:CreativeWork
    328 https://doi.org/10.1073/pnas.0607048103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010227561
    329 rdf:type schema:CreativeWork
    330 https://doi.org/10.1073/pnas.1410657111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005848879
    331 rdf:type schema:CreativeWork
    332 https://doi.org/10.1093/nar/gkt932 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053682623
    333 rdf:type schema:CreativeWork
    334 https://doi.org/10.1099/00221287-146-5-1143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060370362
    335 rdf:type schema:CreativeWork
    336 https://doi.org/10.1099/mic.0.000028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060393943
    337 rdf:type schema:CreativeWork
    338 https://doi.org/10.1099/mic.0.000424 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060394325
    339 rdf:type schema:CreativeWork
    340 https://doi.org/10.1111/febs.13224 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009194241
    341 rdf:type schema:CreativeWork
    342 https://doi.org/10.1111/j.2517-6161.1995.tb02031.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1110458929
    343 rdf:type schema:CreativeWork
    344 https://doi.org/10.1126/science.1246843 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062469181
    345 rdf:type schema:CreativeWork
    346 https://doi.org/10.1128/aem.06876-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036598022
    347 rdf:type schema:CreativeWork
    348 https://doi.org/10.1128/aem.69.11.6500-6506.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046241380
    349 rdf:type schema:CreativeWork
    350 https://doi.org/10.1128/jb.01122-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000647102
    351 rdf:type schema:CreativeWork
    352 https://doi.org/10.1128/jb.171.11.6251-6258.1989 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062717497
    353 rdf:type schema:CreativeWork
    354 https://doi.org/10.1128/jb.183.18.5426-5430.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051333022
    355 rdf:type schema:CreativeWork
    356 https://doi.org/10.1128/jb.183.24.7182-7189.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033933720
    357 rdf:type schema:CreativeWork
    358 https://doi.org/10.1128/jb.186.15.5062-5077.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014327724
    359 rdf:type schema:CreativeWork
    360 https://doi.org/10.1128/jb.187.12.4050-4063.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039524787
    361 rdf:type schema:CreativeWork
    362 https://doi.org/10.1128/jb.187.13.4497-4504.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019615033
    363 rdf:type schema:CreativeWork
    364 https://doi.org/10.1146/annurev.micro.50.1.553 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022415050
    365 rdf:type schema:CreativeWork
    366 https://doi.org/10.1214/aos/1013699998 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036427477
    367 rdf:type schema:CreativeWork
    368 https://doi.org/10.1371/journal.pgen.1002219 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034574117
    369 rdf:type schema:CreativeWork
    370 https://doi.org/10.1371/journal.pone.0134752 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018296689
    371 rdf:type schema:CreativeWork
    372 https://www.grid.ac/institutes/grid.264756.4 schema:alternateName Texas A&M University
    373 schema:name Department of Plant Pathology and Microbiology, Texas A&M University, 77840, College Station, TX, USA
    374 rdf:type schema:Organization
    375 https://www.grid.ac/institutes/grid.30064.31 schema:alternateName Washington State University
    376 schema:name Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, 2710 Crimson Way, 99354, Richland, WA, USA
    377 rdf:type schema:Organization
    378 https://www.grid.ac/institutes/grid.34418.3a schema:alternateName Hubei University
    379 schema:name State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei University, 430062, Wuhan, China
    380 rdf:type schema:Organization
    381 https://www.grid.ac/institutes/grid.451303.0 schema:alternateName Pacific Northwest National Laboratory
    382 schema:name Biological Sciences Division, Pacific Northwest National Laboratory, 99352, Richland, WA, USA
    383 Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, 2710 Crimson Way, 99354, Richland, WA, USA
    384 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...