Ontology type: schema:ScholarlyArticle Open Access: True
2019-12
AUTHORSAmaranta Kahn, Sarah Moraïs, Anastasia P. Galanopoulou, Daehwan Chung, Nicholas S. Sarai, Neal Hengge, Dimitris G. Hatzinikolaou, Michael E. Himmel, Yannick J. Bomble, Edward A. Bayer
ABSTRACTBackground: Renewable energy has become a field of high interest over the past decade, and production of biofuels from cellulosic substrates has a particularly high potential as an alternative source of energy. Industrial deconstruction of biomass, however, is an onerous, exothermic process, the cost of which could be decreased significantly by use of hyperthermophilic enzymes. An efficient way of breaking down cellulosic substrates can also be achieved by highly efficient enzymatic complexes called cellulosomes. The modular architecture of these multi-enzyme complexes results in substrate targeting and proximity-based synergy among the resident enzymes. However, cellulosomes have not been observed in hyperthermophilic bacteria. Results: Here, we report the design and function of a novel hyperthermostable "designer cellulosome" system, which is stable and active at 75 °C. Enzymes from Caldicellulosiruptor bescii, a highly cellulolytic hyperthermophilic anaerobic bacterium, were selected and successfully converted to the cellulosomal mode by grafting onto them divergent dockerin modules that can be inserted in a precise manner into a thermostable chimaeric scaffoldin by virtue of their matching cohesins. Three pairs of cohesins and dockerins, selected from thermophilic microbes, were examined for their stability at extreme temperatures and were determined stable at 75 °C for at least 72 h. The resultant hyperthermostable cellulosome complex exhibited the highest levels of enzymatic activity on microcrystalline cellulose at 75 °C, compared to those of previously reported designer cellulosome systems and the native cellulosome from Clostridium thermocellum. Conclusion: The functional hyperthermophilic platform fulfills the appropriate physico-chemical properties required for exothermic processes. This system can thus be adapted for other types of thermostable enzyme systems and could serve as a basis for a variety of cellulolytic and non-cellulolytic industrial objectives at high temperatures. More... »
PAGES44
http://scigraph.springernature.com/pub.10.1186/s13068-019-1386-y
DOIhttp://dx.doi.org/10.1186/s13068-019-1386-y
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1112463869
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/30858881
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Weizmann Institute of Science",
"id": "https://www.grid.ac/institutes/grid.13992.30",
"name": [
"Department of Biomolecular Sciences, The Weizmann Institute of Science, 7610001, Rehovot, Israel"
],
"type": "Organization"
},
"familyName": "Kahn",
"givenName": "Amaranta",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Ben-Gurion University of the Negev",
"id": "https://www.grid.ac/institutes/grid.7489.2",
"name": [
"Department of Biomolecular Sciences, The Weizmann Institute of Science, 7610001, Rehovot, Israel",
"Faculty of Natural Sciences, Ben-Gurion University of the Negev, 8499000, Beer-Sheva, Israel"
],
"type": "Organization"
},
"familyName": "Mora\u00efs",
"givenName": "Sarah",
"type": "Person"
},
{
"affiliation": {
"alternateName": "National and Kapodistrian University of Athens",
"id": "https://www.grid.ac/institutes/grid.5216.0",
"name": [
"Microbiology Group, Faculty of Biology, National and Kapodistrian University of Athens, Zografou Campus, 15784, Athens, Greece"
],
"type": "Organization"
},
"familyName": "Galanopoulou",
"givenName": "Anastasia P.",
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Renewable Energy Laboratory",
"id": "https://www.grid.ac/institutes/grid.419357.d",
"name": [
"Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA"
],
"type": "Organization"
},
"familyName": "Chung",
"givenName": "Daehwan",
"type": "Person"
},
{
"affiliation": {
"alternateName": "California Institute of Technology",
"id": "https://www.grid.ac/institutes/grid.20861.3d",
"name": [
"Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA",
"Division of Chemistry and Chemical Engineering, California Institute of Technology, 91125, Pasadena, CA, USA"
],
"type": "Organization"
},
"familyName": "Sarai",
"givenName": "Nicholas S.",
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Renewable Energy Laboratory",
"id": "https://www.grid.ac/institutes/grid.419357.d",
"name": [
"Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA"
],
"type": "Organization"
},
"familyName": "Hengge",
"givenName": "Neal",
"type": "Person"
},
{
"affiliation": {
"alternateName": "National and Kapodistrian University of Athens",
"id": "https://www.grid.ac/institutes/grid.5216.0",
"name": [
"Microbiology Group, Faculty of Biology, National and Kapodistrian University of Athens, Zografou Campus, 15784, Athens, Greece"
],
"type": "Organization"
},
"familyName": "Hatzinikolaou",
"givenName": "Dimitris G.",
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Renewable Energy Laboratory",
"id": "https://www.grid.ac/institutes/grid.419357.d",
"name": [
"Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA"
],
"type": "Organization"
},
"familyName": "Himmel",
"givenName": "Michael E.",
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Renewable Energy Laboratory",
"id": "https://www.grid.ac/institutes/grid.419357.d",
"name": [
"Biosciences Center, National Renewable Energy Laboratory, Golden, CO, USA"
],
"type": "Organization"
},
"familyName": "Bomble",
"givenName": "Yannick J.",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Weizmann Institute of Science",
"id": "https://www.grid.ac/institutes/grid.13992.30",
"name": [
"Department of Biomolecular Sciences, The Weizmann Institute of Science, 7610001, Rehovot, Israel"
],
"type": "Organization"
},
"familyName": "Bayer",
"givenName": "Edward A.",
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/978-3-642-30141-4_67",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000389523",
"https://doi.org/10.1007/978-3-642-30141-4_67"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/cctc.201000112",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001346850"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/cctc.201000112",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001346850"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.m414449200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003210169"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aem.00236-09",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003844052"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s12010-015-1711-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003846358",
"https://doi.org/10.1007/s12010-015-1711-2"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.1608012113",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004511020"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/b978-0-12-415931-0.00014-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004864959"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gkq1281",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005671183"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/b978-0-08-088504-9.00167-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006813934"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/ijs.0.017731-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007318682"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/ijs.0.017731-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007318682"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1754-6834-6-182",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008001964",
"https://doi.org/10.1186/1754-6834-6-182"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aem.00398-07",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008404424"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/tcr.20002",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009221343"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0065-2164(10)70001-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009461086"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s13068-014-0142-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010076274",
"https://doi.org/10.1186/s13068-014-0142-6"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s13068-014-0142-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010076274",
"https://doi.org/10.1186/s13068-014-0142-6"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jbiotec.2010.04.012",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010790587"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1471-2164-13-210",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011742550",
"https://doi.org/10.1186/1471-2164-13-210"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.copbio.2009.05.007",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012697130"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/1574-6976.12044",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015234288"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrmicro.2016.164",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015346200",
"https://doi.org/10.1038/nrmicro.2016.164"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mbio.00233-11",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015827600"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.molcatb.2012.09.016",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016767868"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mbio.00083-16",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017866201"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.biortech.2014.07.022",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018405608"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/pmic.200700486",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019025332"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/febs.12032",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019251333"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.copbio.2007.04.004",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020166067"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mmbr.65.1.1-43.2001",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020213941"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0014-5793(95)00074-j",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020411958"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0167-7799(94)90039-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022397567"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1146/annurev.micro.57.030502.091022",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024787833"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aem.07985-11",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024806035"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0012476",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024846309"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mbio.00411-15",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025193940"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gkn663",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025440024"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.1404148111",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026067264"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.2019",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026706357",
"https://doi.org/10.1038/nmeth.2019"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1574-6968.1994.tb07043.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026841852"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1574-6968.1994.tb07043.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026841852"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/0-387-30742-7_19",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029060369",
"https://doi.org/10.1007/0-387-30742-7_19"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00253-016-7594-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030616010",
"https://doi.org/10.1007/s00253-016-7594-5"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1754-6834-7-100",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031120062",
"https://doi.org/10.1186/1754-6834-7-100"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0006650",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031323610"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jbiosc.2012.07.019",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031706059"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0141-0229(92)90153-f",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032199022"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0141-0229(92)90153-f",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032199022"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0966-842x(99)01533-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032593505"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.wasman.2015.07.031",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032636617"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aem.01400-10",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033343050"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/bbb.1724",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033436403"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jmr.749",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035469442"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jmr.749",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035469442"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00172827",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036245456",
"https://doi.org/10.1007/bf00172827"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00172827",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036245456",
"https://doi.org/10.1007/bf00172827"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/1758-2229.12489",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039364103"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4137/ebo.s12539",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039862451"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.biortech.2013.11.024",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039946467"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jb.00256-09",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040391025"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jb.01515-10",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041506613"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aem.01241-09",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042570024"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/srep28504",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042939795",
"https://doi.org/10.1038/srep28504"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s13068-016-0577-z",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043799502",
"https://doi.org/10.1186/s13068-016-0577-z"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s13068-016-0577-z",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043799502",
"https://doi.org/10.1186/s13068-016-0577-z"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4155/bfs.09.25",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044019973"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nsb1097-810",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044672988",
"https://doi.org/10.1038/nsb1097-810"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt0208-169",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045419892",
"https://doi.org/10.1038/nbt0208-169"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/b978-0-12-415931-0.00023-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045527495"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0127326",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045571420"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0119508",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045906425"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aem.02811-10",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046644869"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.copbio.2009.05.005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047141821"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jbiotec.2008.05.003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048048960"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/adma.201503948",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048453928"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.1137016",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049275105"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0084172",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050865671"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/00221287-144-2-457",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060369765"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/ijsem.0.001324",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060389342"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/ijsem.0.001374",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060389392"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.1244273",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062469016"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jb.179.1.46-52.1997",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062725855"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.7868/s0026898413040150",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1074093660"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074253396",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1078336874",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1078587243",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1081613115",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1081868452",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082448044",
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41598-017-08985-w",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1091313366",
"https://doi.org/10.1038/s41598-017-08985-w"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41598-017-08985-w",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1091313366",
"https://doi.org/10.1038/s41598-017-08985-w"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.ijbiomac.2017.08.165",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1091487126"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s13068-017-0898-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1091501464",
"https://doi.org/10.1186/s13068-017-0898-6"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s13068-018-1014-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1100744541",
"https://doi.org/10.1186/s13068-018-1014-2"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.syapm.2018.01.010",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1100960120"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-4939-7877-9_11",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1104335319",
"https://doi.org/10.1007/978-1-4939-7877-9_11"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-4939-7877-9_11",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1104335319",
"https://doi.org/10.1007/978-1-4939-7877-9_11"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-4939-7877-9_12",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1104335439",
"https://doi.org/10.1007/978-1-4939-7877-9_12"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-4939-7877-9_12",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1104335439",
"https://doi.org/10.1007/978-1-4939-7877-9_12"
],
"type": "CreativeWork"
}
],
"datePublished": "2019-12",
"datePublishedReg": "2019-12-01",
"description": "Background: Renewable energy has become a field of high interest over the past decade, and production of biofuels from cellulosic substrates has a particularly high potential as an alternative source of energy. Industrial deconstruction of biomass, however, is an onerous, exothermic process, the cost of which could be decreased significantly by use of hyperthermophilic enzymes. An efficient way of breaking down cellulosic substrates can also be achieved by highly efficient enzymatic complexes called cellulosomes. The modular architecture of these multi-enzyme complexes results in substrate targeting and proximity-based synergy among the resident enzymes. However, cellulosomes have not been observed in hyperthermophilic bacteria.\nResults: Here, we report the design and function of a novel hyperthermostable \"designer cellulosome\" system, which is stable and active at 75\u00a0\u00b0C. Enzymes from Caldicellulosiruptor bescii, a highly cellulolytic hyperthermophilic anaerobic bacterium, were selected and successfully converted to the cellulosomal mode by grafting onto them divergent dockerin modules that can be inserted in a precise manner into a thermostable chimaeric scaffoldin by virtue of their matching cohesins. Three pairs of cohesins and dockerins, selected from thermophilic microbes, were examined for their stability at extreme temperatures and were determined stable at 75\u00a0\u00b0C for at least 72\u00a0h. The resultant hyperthermostable cellulosome complex exhibited the highest levels of enzymatic activity on microcrystalline cellulose at 75\u00a0\u00b0C, compared to those of previously reported designer cellulosome systems and the native cellulosome from Clostridium thermocellum.\nConclusion: The functional hyperthermophilic platform fulfills the appropriate physico-chemical properties required for exothermic processes. This system can thus be adapted for other types of thermostable enzyme systems and could serve as a basis for a variety of cellulolytic and non-cellulolytic industrial objectives at high temperatures.",
"genre": "research_article",
"id": "sg:pub.10.1186/s13068-019-1386-y",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.7173033",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1039046",
"issn": [
"1754-6834"
],
"name": "Biotechnology for Biofuels",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "12"
}
],
"name": "Creation of a functional hyperthermostable designer cellulosome",
"pagination": "44",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"c5f15943e75213aadc5b6f745ecac6d50ba26123343d4857fdf943fa55bb88c9"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"30858881"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"101316935"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1186/s13068-019-1386-y"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1112463869"
]
}
],
"sameAs": [
"https://doi.org/10.1186/s13068-019-1386-y",
"https://app.dimensions.ai/details/publication/pub.1112463869"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T13:19",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000368_0000000368/records_78956_00000001.jsonl",
"type": "ScholarlyArticle",
"url": "https://link.springer.com/10.1186%2Fs13068-019-1386-y"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13068-019-1386-y'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13068-019-1386-y'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13068-019-1386-y'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13068-019-1386-y'
This table displays all metadata directly associated to this object as RDF triples.
418 TRIPLES
21 PREDICATES
118 URIs
21 LITERALS
9 BLANK NODES