Two-stage steam explosion pretreatment of softwood with 2-naphthol as carbocation scavenger View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Christoph-Maximilian Seidel, Simone Brethauer, László Gyenge, Philipp Rudolf von Rohr, Michael H. Studer

ABSTRACT

Background: Lignocellulosic biomass is considered as a potential source for sustainable biofuels. In the conversion process, a pretreatment step is necessary in order to overcome the biomass recalcitrance and allow for sufficient fermentable sugar yields in enzymatic hydrolysis. Steam explosion is a well known pretreatment method working without additional chemicals and allowing for efficient particle size reduction. However, it is not effective for the pretreatment of softwood and the harsh conditions necessary to achieve a highly digestible cellulose fraction lead to the partial degradation of the hemicellulosic sugars. Previous studies showed that the autohydrolysis pretreatreatment of softwood can benefit from the addition of 2-naphthol. This carbocation scavenger prevents lignin repolymerisation leading to an enhanced glucose yield in the subsequent enzymatic hydrolysis. Results: In order to prevent the degradation of the hemicellulose, we investigated in this study a two-stage 2-naphthol steam explosion pretreatment. In the first stage, spruce wood is pretreated at a severity which is optimal for the autocatalytic hydrolysis of the hemicellulose. The hydrolyzate containing the solubilized sugars is withdrawn from the reactor and the remaining solids are pretreated with different amounts of 2-naphthol in a second stage at a severity that allows for high glucose yields in enzymatic hydrolysis. The pretreated spruce was subjected to enzymatic hydrolysis and to simultaneous saccharification and fermentation (SSF). In the first stage, the maximal yield of hemicellulosic sugars was 47.5% at a pretreatment severity of log R 0 = 3.75 at 180 °C. In the second stage, a 2-naphthol dosage of 0.205 mol/mol lignin C9-unit increased the ethanol yield in SSF with a cellulose loading of 1% using the whole second stage pretreatment slurry by 17% from 73.6% for the control without 2-naphthol to 90.4%. At a higher solid loading corresponding to 5% w/w cellulose, the yields decreased due to higher concentrations of residual 2-naphthol in the biomass and the pretreatment liquor, but also due to higher concentrations of potential inhibitors like HMF, furfural and acetic acid. Experiments with washed solids, vacuum filtered solids and the whole slurry showed that residual 2-naphthol can inhibit the fermentation as a single inhibitor but also synergistically together with HMF, furfural and acetic acid. Conclusions: This work shows that a two-stage pretreatment greatly enhances the recovery of hemicellulosic sugars from spruce wood. The presence of 2-naphthol in the second pretreatment stage can enhance the ethanol yield in SSF of steam explosion pretreated softwood at low cellulose concentrations of 1% w/w. However, with higher solid loadings of 5% w/w cellulose, the ethanol yields were in general lower due to the solid effect and a synergistic inhibition of HMF, furfural, acetic acid with residual 2-naphthol. The concentration of residual 2-naphthol tolerated by the yeast decreased with increasing concentrations of HMF, furfural, and acetic acid. More... »

PAGES

37

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13068-019-1373-3

DOI

http://dx.doi.org/10.1186/s13068-019-1373-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112292059

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30828382


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0904", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/09", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Engineering", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Swiss Federal Institute of Technology in Zurich", 
          "id": "https://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Institute of Process Engineering, ETH Z\u00fcrich, Sonneggstrasse 3, 8092, Zurich, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Seidel", 
        "givenName": "Christoph-Maximilian", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Bern University of Applied Sciences", 
          "id": "https://www.grid.ac/institutes/grid.424060.4", 
          "name": [
            "School of Agricultural, Forest and Food Science, Bern University of Applied Science, L\u00e4nggasse 85, 3052, Zollikofen, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brethauer", 
        "givenName": "Simone", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sapientia Hungarian University of Transylvania", 
          "id": "https://www.grid.ac/institutes/grid.270794.f", 
          "name": [
            "School of Agricultural, Forest and Food Science, Bern University of Applied Science, L\u00e4nggasse 85, 3052, Zollikofen, Switzerland", 
            "Faculty of Economics and Socio-Human Sciences and Engineering, Sapientia - Hungarian University of Transylvania, Miercurea Ciuc, Pia\u0163a Libert\u0103\u0163ii nr. 1, 530104, Cluj-Napoca, Romania"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gyenge", 
        "givenName": "L\u00e1szl\u00f3", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Swiss Federal Institute of Technology in Zurich", 
          "id": "https://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Institute of Process Engineering, ETH Z\u00fcrich, Sonneggstrasse 3, 8092, Zurich, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rudolf von Rohr", 
        "givenName": "Philipp", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Bern University of Applied Sciences", 
          "id": "https://www.grid.ac/institutes/grid.424060.4", 
          "name": [
            "School of Agricultural, Forest and Food Science, Bern University of Applied Science, L\u00e4nggasse 85, 3052, Zollikofen, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Studer", 
        "givenName": "Michael H.", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.biortech.2006.10.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001941342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/10_2007_064", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002100978", 
          "https://doi.org/10.1007/10_2007_064"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bp00029a017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002547038"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-002-1058-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002571461", 
          "https://doi.org/10.1007/s00253-002-1058-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/abab:79:1-3:867", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005592131", 
          "https://doi.org/10.1385/abab:79:1-3:867"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.21115", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005729407"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2015.10.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006572531"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13068-016-0567-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008771781", 
          "https://doi.org/10.1186/s13068-016-0567-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13068-016-0567-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008771781", 
          "https://doi.org/10.1186/s13068-016-0567-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ie801542g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010493930"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ie801542g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010493930"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1754-6834-2-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012494166", 
          "https://doi.org/10.1186/1754-6834-2-11"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/hf.2011.071", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014690964"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2007.11.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016138081"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2009.11.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017457802"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1152747", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018211100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4612-1392-5_44", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020369156", 
          "https://doi.org/10.1007/978-1-4612-1392-5_44"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/02773810500191807", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022722809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.procbio.2007.03.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023931982"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2009.11.093", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024630230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260330210", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024682926"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2533/chimia.2015.572", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028881716"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0141-0229(01)00342-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030414743"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12010-011-9221-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034921030", 
          "https://doi.org/10.1007/s12010-011-9221-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c4gc02381a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037443614"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1098/rsta.1987.0029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042130494"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4612-0119-9_1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043742850", 
          "https://doi.org/10.1007/978-1-4612-0119-9_1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4612-0119-9_1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043742850", 
          "https://doi.org/10.1007/978-1-4612-0119-9_1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pecs.2012.03.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045133439"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2004.06.025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045833251"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1137016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049275105"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2009.12.044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049315146"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/abab:84-86:1-9:561", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053030799", 
          "https://doi.org/10.1385/abab:84-86:1-9:561"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13068-017-0816-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085436346", 
          "https://doi.org/10.1186/s13068-017-0816-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13068-017-0816-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085436346", 
          "https://doi.org/10.1186/s13068-017-0816-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/sciadv.1602624", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1086058512"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c7fd00066a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1090281116"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "Background: \u00a0Lignocellulosic biomass is considered as a potential source for sustainable biofuels. In the conversion process, a pretreatment step is necessary in order to overcome the biomass recalcitrance and allow for sufficient fermentable sugar yields in enzymatic hydrolysis. Steam explosion is a well known pretreatment method working without additional chemicals and allowing for efficient particle size reduction. However, it is not effective for the pretreatment of softwood and the harsh conditions necessary to achieve a highly digestible cellulose fraction lead to the partial degradation of the hemicellulosic sugars. Previous studies showed that the autohydrolysis pretreatreatment of softwood can benefit from the addition of 2-naphthol. This carbocation scavenger prevents lignin repolymerisation leading to an enhanced glucose yield in the subsequent enzymatic hydrolysis.\nResults: \u00a0In order to prevent the degradation of the hemicellulose, we investigated in this study a two-stage 2-naphthol steam explosion pretreatment. In the first stage, spruce wood is pretreated at a severity which is optimal for the autocatalytic hydrolysis of the hemicellulose. The hydrolyzate containing the solubilized sugars is withdrawn from the reactor and the remaining solids are pretreated with different amounts of 2-naphthol in a second stage at a severity that allows for high glucose yields in enzymatic hydrolysis. The pretreated spruce was subjected to enzymatic hydrolysis and to simultaneous saccharification and fermentation (SSF). In the first stage, the maximal yield of hemicellulosic sugars was 47.5% at a pretreatment severity of log R 0  \u2009=\u20093.75 at 180\u00a0\u00b0C. In the second stage, a 2-naphthol dosage of 0.205\u00a0mol/mol lignin C9-unit increased the ethanol yield in SSF with a cellulose loading of 1% using the whole second stage pretreatment slurry by 17% from 73.6% for the control without 2-naphthol to 90.4%. At a higher solid loading corresponding to 5% w/w cellulose, the yields decreased due to higher concentrations of residual 2-naphthol in the biomass and the pretreatment liquor, but also due to higher concentrations of potential inhibitors like HMF, furfural and acetic acid. Experiments with washed solids, vacuum filtered solids and the whole slurry showed that residual 2-naphthol can inhibit the fermentation as a single inhibitor but also synergistically together with HMF, furfural and acetic acid.\nConclusions: \u00a0This work shows that a two-stage pretreatment greatly enhances the recovery of hemicellulosic sugars from spruce wood. The presence of 2-naphthol in the second pretreatment stage can enhance the ethanol yield in SSF of steam explosion pretreated softwood at low cellulose concentrations of 1% w/w. However, with higher solid loadings of 5% w/w cellulose, the ethanol yields were in general lower due to the solid effect and a synergistic inhibition of HMF, furfural, acetic acid with residual 2-naphthol. The concentration of residual 2-naphthol tolerated by the yeast decreased with increasing concentrations of HMF, furfural, and acetic acid.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s13068-019-1373-3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1039046", 
        "issn": [
          "1754-6834"
        ], 
        "name": "Biotechnology for Biofuels", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "12"
      }
    ], 
    "name": "Two-stage steam explosion pretreatment of softwood with 2-naphthol as carbocation scavenger", 
    "pagination": "37", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "c436194660c998227987f1393fbd61cbdd8b342a5db037e6b382a8c06b379e8a"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30828382"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101316935"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s13068-019-1373-3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112292059"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s13068-019-1373-3", 
      "https://app.dimensions.ai/details/publication/pub.1112292059"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000360_0000000360/records_118309_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs13068-019-1373-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13068-019-1373-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13068-019-1373-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13068-019-1373-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13068-019-1373-3'


 

This table displays all metadata directly associated to this object as RDF triples.

207 TRIPLES      21 PREDICATES      62 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s13068-019-1373-3 schema:about anzsrc-for:09
2 anzsrc-for:0904
3 schema:author Ne3e4ae2b13fa4e90a1ee9e5146c2e89c
4 schema:citation sg:pub.10.1007/10_2007_064
5 sg:pub.10.1007/978-1-4612-0119-9_1
6 sg:pub.10.1007/978-1-4612-1392-5_44
7 sg:pub.10.1007/s00253-002-1058-9
8 sg:pub.10.1007/s12010-011-9221-3
9 sg:pub.10.1186/1754-6834-2-11
10 sg:pub.10.1186/s13068-016-0567-1
11 sg:pub.10.1186/s13068-017-0816-y
12 sg:pub.10.1385/abab:79:1-3:867
13 sg:pub.10.1385/abab:84-86:1-9:561
14 https://doi.org/10.1002/bit.21115
15 https://doi.org/10.1002/bit.260330210
16 https://doi.org/10.1016/j.biortech.2004.06.025
17 https://doi.org/10.1016/j.biortech.2006.10.018
18 https://doi.org/10.1016/j.biortech.2007.11.013
19 https://doi.org/10.1016/j.biortech.2009.11.007
20 https://doi.org/10.1016/j.biortech.2009.11.093
21 https://doi.org/10.1016/j.biortech.2009.12.044
22 https://doi.org/10.1016/j.biortech.2015.10.009
23 https://doi.org/10.1016/j.pecs.2012.03.002
24 https://doi.org/10.1016/j.procbio.2007.03.012
25 https://doi.org/10.1016/s0141-0229(01)00342-8
26 https://doi.org/10.1021/bp00029a017
27 https://doi.org/10.1021/ie801542g
28 https://doi.org/10.1039/c4gc02381a
29 https://doi.org/10.1039/c7fd00066a
30 https://doi.org/10.1080/02773810500191807
31 https://doi.org/10.1098/rsta.1987.0029
32 https://doi.org/10.1126/sciadv.1602624
33 https://doi.org/10.1126/science.1137016
34 https://doi.org/10.1126/science.1152747
35 https://doi.org/10.1515/hf.2011.071
36 https://doi.org/10.2533/chimia.2015.572
37 schema:datePublished 2019-12
38 schema:datePublishedReg 2019-12-01
39 schema:description Background:  Lignocellulosic biomass is considered as a potential source for sustainable biofuels. In the conversion process, a pretreatment step is necessary in order to overcome the biomass recalcitrance and allow for sufficient fermentable sugar yields in enzymatic hydrolysis. Steam explosion is a well known pretreatment method working without additional chemicals and allowing for efficient particle size reduction. However, it is not effective for the pretreatment of softwood and the harsh conditions necessary to achieve a highly digestible cellulose fraction lead to the partial degradation of the hemicellulosic sugars. Previous studies showed that the autohydrolysis pretreatreatment of softwood can benefit from the addition of 2-naphthol. This carbocation scavenger prevents lignin repolymerisation leading to an enhanced glucose yield in the subsequent enzymatic hydrolysis. Results:  In order to prevent the degradation of the hemicellulose, we investigated in this study a two-stage 2-naphthol steam explosion pretreatment. In the first stage, spruce wood is pretreated at a severity which is optimal for the autocatalytic hydrolysis of the hemicellulose. The hydrolyzate containing the solubilized sugars is withdrawn from the reactor and the remaining solids are pretreated with different amounts of 2-naphthol in a second stage at a severity that allows for high glucose yields in enzymatic hydrolysis. The pretreated spruce was subjected to enzymatic hydrolysis and to simultaneous saccharification and fermentation (SSF). In the first stage, the maximal yield of hemicellulosic sugars was 47.5% at a pretreatment severity of log R 0  = 3.75 at 180 °C. In the second stage, a 2-naphthol dosage of 0.205 mol/mol lignin C9-unit increased the ethanol yield in SSF with a cellulose loading of 1% using the whole second stage pretreatment slurry by 17% from 73.6% for the control without 2-naphthol to 90.4%. At a higher solid loading corresponding to 5% w/w cellulose, the yields decreased due to higher concentrations of residual 2-naphthol in the biomass and the pretreatment liquor, but also due to higher concentrations of potential inhibitors like HMF, furfural and acetic acid. Experiments with washed solids, vacuum filtered solids and the whole slurry showed that residual 2-naphthol can inhibit the fermentation as a single inhibitor but also synergistically together with HMF, furfural and acetic acid. Conclusions:  This work shows that a two-stage pretreatment greatly enhances the recovery of hemicellulosic sugars from spruce wood. The presence of 2-naphthol in the second pretreatment stage can enhance the ethanol yield in SSF of steam explosion pretreated softwood at low cellulose concentrations of 1% w/w. However, with higher solid loadings of 5% w/w cellulose, the ethanol yields were in general lower due to the solid effect and a synergistic inhibition of HMF, furfural, acetic acid with residual 2-naphthol. The concentration of residual 2-naphthol tolerated by the yeast decreased with increasing concentrations of HMF, furfural, and acetic acid.
40 schema:genre research_article
41 schema:inLanguage en
42 schema:isAccessibleForFree true
43 schema:isPartOf Nd940cdeb281742ffb85112d502ac2783
44 Ne4ff1ee4897f4cec831d2b1468d16caf
45 sg:journal.1039046
46 schema:name Two-stage steam explosion pretreatment of softwood with 2-naphthol as carbocation scavenger
47 schema:pagination 37
48 schema:productId N08bcffce04c94c5a9bea87b93273353f
49 N41b36b55773c414e8692b44418bfa2e4
50 Nbb70e47f8ce44377a203ead1db048610
51 Nd4abed6f5649428abf45aca80fef7d8f
52 Nf665eb6f29ae4920bf0fd5aee41e59c2
53 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112292059
54 https://doi.org/10.1186/s13068-019-1373-3
55 schema:sdDatePublished 2019-04-11T12:04
56 schema:sdLicense https://scigraph.springernature.com/explorer/license/
57 schema:sdPublisher Na879ad3c4a2e4898bad657de199ba0ae
58 schema:url https://link.springer.com/10.1186%2Fs13068-019-1373-3
59 sgo:license sg:explorer/license/
60 sgo:sdDataset articles
61 rdf:type schema:ScholarlyArticle
62 N08bcffce04c94c5a9bea87b93273353f schema:name pubmed_id
63 schema:value 30828382
64 rdf:type schema:PropertyValue
65 N155ac799379e42ef885a083e3e5167d7 schema:affiliation https://www.grid.ac/institutes/grid.424060.4
66 schema:familyName Brethauer
67 schema:givenName Simone
68 rdf:type schema:Person
69 N41b36b55773c414e8692b44418bfa2e4 schema:name dimensions_id
70 schema:value pub.1112292059
71 rdf:type schema:PropertyValue
72 N61d404f1efd34e43b4ad56626bc2909c schema:affiliation https://www.grid.ac/institutes/grid.5801.c
73 schema:familyName Seidel
74 schema:givenName Christoph-Maximilian
75 rdf:type schema:Person
76 Na0d91188e481449fa9df67a3b0242bd2 schema:affiliation https://www.grid.ac/institutes/grid.424060.4
77 schema:familyName Studer
78 schema:givenName Michael H.
79 rdf:type schema:Person
80 Na879ad3c4a2e4898bad657de199ba0ae schema:name Springer Nature - SN SciGraph project
81 rdf:type schema:Organization
82 Nb0f72231d1f24007ac9200ea5f687ed6 rdf:first N155ac799379e42ef885a083e3e5167d7
83 rdf:rest Nbc3874b8cd9640f69707f5962fe41727
84 Nbb70e47f8ce44377a203ead1db048610 schema:name nlm_unique_id
85 schema:value 101316935
86 rdf:type schema:PropertyValue
87 Nbc3874b8cd9640f69707f5962fe41727 rdf:first Nfe61182a5c314994a561864f2551d76e
88 rdf:rest Neabff5bec3b74065b73f4b0d0d9b55b9
89 Nd4abed6f5649428abf45aca80fef7d8f schema:name doi
90 schema:value 10.1186/s13068-019-1373-3
91 rdf:type schema:PropertyValue
92 Nd940cdeb281742ffb85112d502ac2783 schema:volumeNumber 12
93 rdf:type schema:PublicationVolume
94 Ne3e4ae2b13fa4e90a1ee9e5146c2e89c rdf:first N61d404f1efd34e43b4ad56626bc2909c
95 rdf:rest Nb0f72231d1f24007ac9200ea5f687ed6
96 Ne4ff1ee4897f4cec831d2b1468d16caf schema:issueNumber 1
97 rdf:type schema:PublicationIssue
98 Neabff5bec3b74065b73f4b0d0d9b55b9 rdf:first Nf213fc0fb42642e293641ac6a737cba1
99 rdf:rest Neee4f23837ce4f87a6555494e05fc817
100 Neee4f23837ce4f87a6555494e05fc817 rdf:first Na0d91188e481449fa9df67a3b0242bd2
101 rdf:rest rdf:nil
102 Nf213fc0fb42642e293641ac6a737cba1 schema:affiliation https://www.grid.ac/institutes/grid.5801.c
103 schema:familyName Rudolf von Rohr
104 schema:givenName Philipp
105 rdf:type schema:Person
106 Nf665eb6f29ae4920bf0fd5aee41e59c2 schema:name readcube_id
107 schema:value c436194660c998227987f1393fbd61cbdd8b342a5db037e6b382a8c06b379e8a
108 rdf:type schema:PropertyValue
109 Nfe61182a5c314994a561864f2551d76e schema:affiliation https://www.grid.ac/institutes/grid.270794.f
110 schema:familyName Gyenge
111 schema:givenName László
112 rdf:type schema:Person
113 anzsrc-for:09 schema:inDefinedTermSet anzsrc-for:
114 schema:name Engineering
115 rdf:type schema:DefinedTerm
116 anzsrc-for:0904 schema:inDefinedTermSet anzsrc-for:
117 schema:name Chemical Engineering
118 rdf:type schema:DefinedTerm
119 sg:journal.1039046 schema:issn 1754-6834
120 schema:name Biotechnology for Biofuels
121 rdf:type schema:Periodical
122 sg:pub.10.1007/10_2007_064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002100978
123 https://doi.org/10.1007/10_2007_064
124 rdf:type schema:CreativeWork
125 sg:pub.10.1007/978-1-4612-0119-9_1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043742850
126 https://doi.org/10.1007/978-1-4612-0119-9_1
127 rdf:type schema:CreativeWork
128 sg:pub.10.1007/978-1-4612-1392-5_44 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020369156
129 https://doi.org/10.1007/978-1-4612-1392-5_44
130 rdf:type schema:CreativeWork
131 sg:pub.10.1007/s00253-002-1058-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002571461
132 https://doi.org/10.1007/s00253-002-1058-9
133 rdf:type schema:CreativeWork
134 sg:pub.10.1007/s12010-011-9221-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034921030
135 https://doi.org/10.1007/s12010-011-9221-3
136 rdf:type schema:CreativeWork
137 sg:pub.10.1186/1754-6834-2-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012494166
138 https://doi.org/10.1186/1754-6834-2-11
139 rdf:type schema:CreativeWork
140 sg:pub.10.1186/s13068-016-0567-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008771781
141 https://doi.org/10.1186/s13068-016-0567-1
142 rdf:type schema:CreativeWork
143 sg:pub.10.1186/s13068-017-0816-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1085436346
144 https://doi.org/10.1186/s13068-017-0816-y
145 rdf:type schema:CreativeWork
146 sg:pub.10.1385/abab:79:1-3:867 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005592131
147 https://doi.org/10.1385/abab:79:1-3:867
148 rdf:type schema:CreativeWork
149 sg:pub.10.1385/abab:84-86:1-9:561 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053030799
150 https://doi.org/10.1385/abab:84-86:1-9:561
151 rdf:type schema:CreativeWork
152 https://doi.org/10.1002/bit.21115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005729407
153 rdf:type schema:CreativeWork
154 https://doi.org/10.1002/bit.260330210 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024682926
155 rdf:type schema:CreativeWork
156 https://doi.org/10.1016/j.biortech.2004.06.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045833251
157 rdf:type schema:CreativeWork
158 https://doi.org/10.1016/j.biortech.2006.10.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001941342
159 rdf:type schema:CreativeWork
160 https://doi.org/10.1016/j.biortech.2007.11.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016138081
161 rdf:type schema:CreativeWork
162 https://doi.org/10.1016/j.biortech.2009.11.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017457802
163 rdf:type schema:CreativeWork
164 https://doi.org/10.1016/j.biortech.2009.11.093 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024630230
165 rdf:type schema:CreativeWork
166 https://doi.org/10.1016/j.biortech.2009.12.044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049315146
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1016/j.biortech.2015.10.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006572531
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1016/j.pecs.2012.03.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045133439
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1016/j.procbio.2007.03.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023931982
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1016/s0141-0229(01)00342-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030414743
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1021/bp00029a017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002547038
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1021/ie801542g schema:sameAs https://app.dimensions.ai/details/publication/pub.1010493930
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1039/c4gc02381a schema:sameAs https://app.dimensions.ai/details/publication/pub.1037443614
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1039/c7fd00066a schema:sameAs https://app.dimensions.ai/details/publication/pub.1090281116
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1080/02773810500191807 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022722809
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1098/rsta.1987.0029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042130494
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1126/sciadv.1602624 schema:sameAs https://app.dimensions.ai/details/publication/pub.1086058512
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1126/science.1137016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049275105
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1126/science.1152747 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018211100
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1515/hf.2011.071 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014690964
195 rdf:type schema:CreativeWork
196 https://doi.org/10.2533/chimia.2015.572 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028881716
197 rdf:type schema:CreativeWork
198 https://www.grid.ac/institutes/grid.270794.f schema:alternateName Sapientia Hungarian University of Transylvania
199 schema:name Faculty of Economics and Socio-Human Sciences and Engineering, Sapientia - Hungarian University of Transylvania, Miercurea Ciuc, Piaţa Libertăţii nr. 1, 530104, Cluj-Napoca, Romania
200 School of Agricultural, Forest and Food Science, Bern University of Applied Science, Länggasse 85, 3052, Zollikofen, Switzerland
201 rdf:type schema:Organization
202 https://www.grid.ac/institutes/grid.424060.4 schema:alternateName Bern University of Applied Sciences
203 schema:name School of Agricultural, Forest and Food Science, Bern University of Applied Science, Länggasse 85, 3052, Zollikofen, Switzerland
204 rdf:type schema:Organization
205 https://www.grid.ac/institutes/grid.5801.c schema:alternateName Swiss Federal Institute of Technology in Zurich
206 schema:name Institute of Process Engineering, ETH Zürich, Sonneggstrasse 3, 8092, Zurich, Switzerland
207 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...