Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-12

AUTHORS

Christopher D. Herring, William R. Kenealy, A. Joe Shaw, Sean F. Covalla, Daniel G. Olson, Jiayi Zhang, W. Ryan Sillers, Vasiliki Tsakraklides, John S. Bardsley, Stephen R. Rogers, Philip G. Thorne, Jessica P. Johnson, Abigail Foster, Indraneel D. Shikhare, Dawn M. Klingeman, Steven D. Brown, Brian H. Davison, Lee R. Lynd, David A. Hogsett

ABSTRACT

BACKGROUND: The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equivalent to yeast. While saccharolytic anaerobes have been long studied as potential biomass-fermenting organisms, development efforts for commercial ethanol production have not been reported. RESULTS: Here, we describe the highest ethanol titers achieved from T. saccharolyticum during a 4-year project to develop it for industrial production of ethanol from pre-treated hardwood at 51-55 °C. We describe organism and bioprocess development efforts undertaken to improve ethanol production. The final strain M2886 was generated by removing genes for exopolysaccharide synthesis, the regulator perR, and re-introduction of phosphotransacetylase and acetate kinase into the methyglyoxal synthase gene. It was also subject to multiple rounds of adaptation and selection, resulting in mutations later identified by resequencing. The highest ethanol titer achieved was 70 g/L in batch culture with a mixture of cellobiose and maltodextrin. In a "mock hydrolysate" Simultaneous Saccharification and Fermentation (SSF) with Sigmacell-20, glucose, xylose, and acetic acid, an ethanol titer of 61 g/L was achieved, at 92 % of theoretical yield. Fungal cellulases were rapidly inactivated under these conditions and had to be supplemented with cellulosomes from C. thermocellum. Ethanol titers of 31 g/L were reached in a 100 L SSF of pre-treated hardwood and 26 g/L in a fermentation of a hardwood hemicellulose extract. CONCLUSIONS: This study demonstrates that thermophilic anaerobes are capable of producing ethanol at high yield and at titers greater than 60 g/L from purified substrates, but additional work is needed to produce the same ethanol titers from pre-treated hardwood. More... »

PAGES

125

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13068-016-0536-8

DOI

http://dx.doi.org/10.1186/s13068-016-0536-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002454126

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27313661


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    "description": "BACKGROUND: The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equivalent to yeast. While saccharolytic anaerobes have been long studied as potential biomass-fermenting organisms, development efforts for commercial ethanol production have not been reported.\nRESULTS: Here, we describe the highest ethanol titers achieved from T. saccharolyticum during a 4-year project to develop it for industrial production of ethanol from pre-treated hardwood at 51-55\u00a0\u00b0C. We describe organism and bioprocess development efforts undertaken to improve ethanol production. The final strain M2886 was generated by removing genes for exopolysaccharide synthesis, the regulator perR, and re-introduction of phosphotransacetylase and acetate kinase into the methyglyoxal synthase gene. It was also subject to multiple rounds of adaptation and selection, resulting in mutations later identified by resequencing. The highest ethanol titer achieved was 70\u00a0g/L in batch culture with a mixture of cellobiose and maltodextrin. In a \"mock hydrolysate\" Simultaneous Saccharification and Fermentation (SSF) with Sigmacell-20, glucose, xylose, and acetic acid, an ethanol titer of 61\u00a0g/L was achieved, at 92\u00a0% of theoretical yield. Fungal cellulases were rapidly inactivated under these conditions and had to be supplemented with cellulosomes from C. thermocellum. Ethanol titers of 31\u00a0g/L were reached in a 100\u00a0L SSF of pre-treated hardwood and 26\u00a0g/L in a fermentation of a hardwood hemicellulose extract.\nCONCLUSIONS: This study demonstrates that thermophilic anaerobes are capable of producing ethanol at high yield and at titers greater than 60\u00a0g/L from purified substrates, but additional work is needed to produce the same ethanol titers from pre-treated hardwood.", 
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307 Novozymes Inc, Davis, CA, USA
308 rdf:type schema:Organization
309 https://www.grid.ac/institutes/grid.439009.7 schema:alternateName Verdezyne (United States)
310 schema:name Mascoma Corporation, 67 Etna Rd, 03766, Lebanon, NH, USA
311 Verdezyne, Carlsbad, CA, USA
312 rdf:type schema:Organization
 




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