The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-12

AUTHORS

Judith Rumin, Hubert Bonnefond, Bruno Saint-Jean, Catherine Rouxel, Antoine Sciandra, Olivier Bernard, Jean-Paul Cadoret, Gaël Bougaran

ABSTRACT

Microalgae are currently emerging as one of the most promising alternative sources for the next generation of food, feed, cosmetics and renewable energy in the form of biofuel. Microalgae constitute a diverse group of microorganisms with advantages like fast and efficient growth. In addition, they do not compete for arable land and offer very high lipid yield potential. Major challenges for the development of this resource are to select lipid-rich strains using high-throughput staining for neutral lipid content in microalgae species. For this purpose, the fluorescent dyes most commonly used to quantify lipids are Nile red and BODIPY 505/515. Their fluorescent staining for lipids offers a rapid and inexpensive analysis tool to measure neutral lipid content, avoiding time-consuming and costly gravimetric analysis. This review collates and presents recent advances in algal lipid staining and focuses on Nile red and BODIPY 505/515 staining characteristics. The available literature addresses the limitations of fluorescent dyes under certain conditions, such as spectral properties, dye concentrations, cell concentrations, temperature and incubation duration. Moreover, the overall conclusion of the present review study gives limitations on the use of fluorochrome for screening of lipid-rich microalgae species and suggests improved protocols for staining recalcitrant microalgae and recommendations for the staining quantification. More... »

PAGES

42

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13068-015-0220-4

    DOI

    http://dx.doi.org/10.1186/s13068-015-0220-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1025480930

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/25788982


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