Isolation of polysaccharides from Dendrobium officinale leaves and anti-inflammatory activity in LPS-stimulated THP-1 cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Min Zhang, Junwen Wu, Juanjuan Han, Hongmei Shu, Kehai Liu

ABSTRACT

Dendrobium officinale stem is rich in polysaccharides, which play a great role in the medicinal effects of this plant. However, little was known about the polysaccharides from Dendrobium officinale leaves. Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column). The average molecular weights were determined as 28,342 Da and 41,143 Da, respectively. Monosaccharide compositions were analyzed using gas chromatography-mass spectrometer. DLP-1 was composed of D-(+)-galactose, DL-arabinose, and L-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of D-xylose, D-glucose, and D-(+)-mannose. DLP-2 was consisted of D-glucose and D-(+)-galactose with a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose. Then, we established inflammatory cell model by LPS acting THP-1 cells to investigate the anti-inflammatory effects of DLP-1 and DLP-2. The results indicated that DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) were effective in protecting THP-1 cells from LPS-stimulated cytotoxicity, as well as inhibiting reactive oxygen species formation. In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells. More... »

PAGES

109

Journal

TITLE

Chemistry Central Journal

ISSUE

1

VOLUME

12

Author Affiliations

From Grant

  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13065-018-0480-8

    DOI

    http://dx.doi.org/10.1186/s13065-018-0480-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1107931528

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30377844


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Shanghai Ocean University", 
              "id": "https://www.grid.ac/institutes/grid.412514.7", 
              "name": [
                "Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, 999 Hucheng Ring Road, Lingang New City, 201306, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Min", 
            "id": "sg:person.016055775037.93", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016055775037.93"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Shanghai Ocean University", 
              "id": "https://www.grid.ac/institutes/grid.412514.7", 
              "name": [
                "Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, 999 Hucheng Ring Road, Lingang New City, 201306, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wu", 
            "givenName": "Junwen", 
            "id": "sg:person.014463034037.52", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014463034037.52"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Shanghai Ocean University", 
              "id": "https://www.grid.ac/institutes/grid.412514.7", 
              "name": [
                "Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, 999 Hucheng Ring Road, Lingang New City, 201306, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Han", 
            "givenName": "Juanjuan", 
            "id": "sg:person.07356564737.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07356564737.39"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Shanghai Ocean University", 
              "id": "https://www.grid.ac/institutes/grid.412514.7", 
              "name": [
                "Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, 999 Hucheng Ring Road, Lingang New City, 201306, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shu", 
            "givenName": "Hongmei", 
            "id": "sg:person.014613330015.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014613330015.13"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Shanghai Ocean University", 
              "id": "https://www.grid.ac/institutes/grid.412514.7", 
              "name": [
                "Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, 999 Hucheng Ring Road, Lingang New City, 201306, Shanghai, China", 
                "National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, 201306, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Liu", 
            "givenName": "Kehai", 
            "id": "sg:person.01144310126.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144310126.10"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/s11427-012-4419-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003350962", 
              "https://doi.org/10.1007/s11427-012-4419-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11557-011-0754-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006656578", 
              "https://doi.org/10.1007/s11557-011-0754-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.foodres.2015.01.035", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007126181"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/intimm/dxh186", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009264145"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bcdf.2014.12.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009364874"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bcdf.2014.12.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011769883"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.intimp.2014.08.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013267497"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jff.2011.12.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016712715"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molimm.2014.06.033", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019799403"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-011-3829-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025736393", 
              "https://doi.org/10.1007/s00253-011-3829-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09121", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028162986", 
              "https://doi.org/10.1038/nature09121"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09121", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028162986", 
              "https://doi.org/10.1038/nature09121"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/c0fo00113a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030195397"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/c0fo00113a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030195397"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.carbpol.2015.05.079", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030269541"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.carbpol.2015.05.079", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030269541"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.carbpol.2015.05.079", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030269541"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.carbpol.2015.05.079", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030269541"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jep.2008.11.029", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033462545"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1124/pr.109.001073", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034974999"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m007386200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037409651"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/shk.0000000000000042", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039807186"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/shk.0000000000000042", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039807186"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ijbiomac.2015.06.023", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042201079"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00005-013-0243-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043410833", 
              "https://doi.org/10.1007/s00005-013-0243-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00005-013-0243-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043410833", 
              "https://doi.org/10.1007/s00005-013-0243-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ijbiomac.2015.11.038", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043994437"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.intimp.2016.08.026", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044758320"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ijbiomac.2016.04.067", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049767504"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.intimp.2014.08.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051163784"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/acs.jafc.5b05180", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055095633"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4268/cjcmm20140508", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072428446"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1078432181", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1934578x1100600627", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1078432181"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1934578x1100600627", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1078432181"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jep.2017.08.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090954000"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13065-017-0349-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092827141", 
              "https://doi.org/10.1186/s13065-017-0349-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3390/md15120388", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1099831001"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13065-018-0468-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1107224898", 
              "https://doi.org/10.1186/s13065-018-0468-4"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2018-12", 
        "datePublishedReg": "2018-12-01", 
        "description": "Dendrobium officinale stem is rich in polysaccharides, which play a great role in the medicinal effects of this plant. However, little was known about the polysaccharides from Dendrobium officinale leaves. Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column). The average molecular weights were determined as 28,342\u00a0Da and 41,143\u00a0Da, respectively. Monosaccharide compositions were analyzed using gas chromatography-mass spectrometer. DLP-1 was composed of D-(+)-galactose, DL-arabinose, and L-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of D-xylose, D-glucose, and D-(+)-mannose. DLP-2 was consisted of D-glucose and D-(+)-galactose with a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose. Then, we established inflammatory\u00a0cell\u00a0model by\u00a0LPS acting THP-1 cells to investigate the anti-inflammatory effects of DLP-1 and DLP-2. The results indicated that DLP-1 (5\u00a0\u03bcg/mL) and DLP-2 (50\u00a0\u03bcg/mL) were effective in protecting THP-1 cells from LPS-stimulated cytotoxicity, as well as inhibiting reactive oxygen species formation. In addition, both DLP-1 (5\u00a0\u03bcg/mL) and DLP-2 (50\u00a0\u03bcg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1186/s13065-018-0480-8", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.5015126", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1039133", 
            "issn": [
              "1864-6158", 
              "2191-2858"
            ], 
            "name": "Chemistry Central Journal", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "12"
          }
        ], 
        "name": "Isolation of polysaccharides from Dendrobium officinale leaves and anti-inflammatory activity in LPS-stimulated THP-1 cells", 
        "pagination": "109", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "6a84f0ebde355d3bfe83dc9e76facb44ae8d36e84ab9b499251019b4b3d04e15"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30377844"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101314213"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/s13065-018-0480-8"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1107931528"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/s13065-018-0480-8", 
          "https://app.dimensions.ai/details/publication/pub.1107931528"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T17:41", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8672_00000573.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://link.springer.com/10.1186%2Fs13065-018-0480-8"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13065-018-0480-8'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13065-018-0480-8'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13065-018-0480-8'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13065-018-0480-8'


     

    This table displays all metadata directly associated to this object as RDF triples.

    199 TRIPLES      21 PREDICATES      60 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/s13065-018-0480-8 schema:about anzsrc-for:06
    2 anzsrc-for:0604
    3 schema:author N9787fe34e3284a60bb65a49eccf7ce5a
    4 schema:citation sg:pub.10.1007/s00005-013-0243-0
    5 sg:pub.10.1007/s00253-011-3829-7
    6 sg:pub.10.1007/s11427-012-4419-3
    7 sg:pub.10.1007/s11557-011-0754-1
    8 sg:pub.10.1038/nature09121
    9 sg:pub.10.1186/s13065-017-0349-2
    10 sg:pub.10.1186/s13065-018-0468-4
    11 https://app.dimensions.ai/details/publication/pub.1078432181
    12 https://doi.org/10.1016/j.bcdf.2014.12.001
    13 https://doi.org/10.1016/j.bcdf.2014.12.002
    14 https://doi.org/10.1016/j.carbpol.2015.05.079
    15 https://doi.org/10.1016/j.foodres.2015.01.035
    16 https://doi.org/10.1016/j.ijbiomac.2015.06.023
    17 https://doi.org/10.1016/j.ijbiomac.2015.11.038
    18 https://doi.org/10.1016/j.ijbiomac.2016.04.067
    19 https://doi.org/10.1016/j.intimp.2014.08.002
    20 https://doi.org/10.1016/j.intimp.2014.08.011
    21 https://doi.org/10.1016/j.intimp.2016.08.026
    22 https://doi.org/10.1016/j.jep.2008.11.029
    23 https://doi.org/10.1016/j.jep.2017.08.001
    24 https://doi.org/10.1016/j.jff.2011.12.006
    25 https://doi.org/10.1016/j.molimm.2014.06.033
    26 https://doi.org/10.1021/acs.jafc.5b05180
    27 https://doi.org/10.1039/c0fo00113a
    28 https://doi.org/10.1074/jbc.m007386200
    29 https://doi.org/10.1093/intimm/dxh186
    30 https://doi.org/10.1097/shk.0000000000000042
    31 https://doi.org/10.1124/pr.109.001073
    32 https://doi.org/10.1177/1934578x1100600627
    33 https://doi.org/10.3390/md15120388
    34 https://doi.org/10.4268/cjcmm20140508
    35 schema:datePublished 2018-12
    36 schema:datePublishedReg 2018-12-01
    37 schema:description Dendrobium officinale stem is rich in polysaccharides, which play a great role in the medicinal effects of this plant. However, little was known about the polysaccharides from Dendrobium officinale leaves. Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column). The average molecular weights were determined as 28,342 Da and 41,143 Da, respectively. Monosaccharide compositions were analyzed using gas chromatography-mass spectrometer. DLP-1 was composed of D-(+)-galactose, DL-arabinose, and L-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of D-xylose, D-glucose, and D-(+)-mannose. DLP-2 was consisted of D-glucose and D-(+)-galactose with a molar ratio of 3.23:1.02, and traces of D-xylose, DL-arabinose. Then, we established inflammatory cell model by LPS acting THP-1 cells to investigate the anti-inflammatory effects of DLP-1 and DLP-2. The results indicated that DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) were effective in protecting THP-1 cells from LPS-stimulated cytotoxicity, as well as inhibiting reactive oxygen species formation. In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
    38 schema:genre research_article
    39 schema:inLanguage en
    40 schema:isAccessibleForFree true
    41 schema:isPartOf N9c7d2bfd198c4edb8c40d8ef1e66088c
    42 Ndaa4eca69dcf49dc8ccec119cbfb96ce
    43 sg:journal.1039133
    44 schema:name Isolation of polysaccharides from Dendrobium officinale leaves and anti-inflammatory activity in LPS-stimulated THP-1 cells
    45 schema:pagination 109
    46 schema:productId N03212d151b764778a387b5634dcf01cd
    47 N1c701331de0f4029824fd3f2b60706e4
    48 N3853a8c5da074aefb1c302bbac53631d
    49 N44661d98f51a47f482152f02110cb786
    50 Nb8f5d43b0a0445b588e161d8aa24ef82
    51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107931528
    52 https://doi.org/10.1186/s13065-018-0480-8
    53 schema:sdDatePublished 2019-04-10T17:41
    54 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    55 schema:sdPublisher N6b60a553cc0349129b53b02e47ccdb39
    56 schema:url https://link.springer.com/10.1186%2Fs13065-018-0480-8
    57 sgo:license sg:explorer/license/
    58 sgo:sdDataset articles
    59 rdf:type schema:ScholarlyArticle
    60 N03212d151b764778a387b5634dcf01cd schema:name nlm_unique_id
    61 schema:value 101314213
    62 rdf:type schema:PropertyValue
    63 N05d77ad851034b21950589616eb0b51c rdf:first sg:person.014463034037.52
    64 rdf:rest Nfb2a5d038e9446cd97c0df272747b3fe
    65 N1c701331de0f4029824fd3f2b60706e4 schema:name pubmed_id
    66 schema:value 30377844
    67 rdf:type schema:PropertyValue
    68 N3853a8c5da074aefb1c302bbac53631d schema:name doi
    69 schema:value 10.1186/s13065-018-0480-8
    70 rdf:type schema:PropertyValue
    71 N44661d98f51a47f482152f02110cb786 schema:name dimensions_id
    72 schema:value pub.1107931528
    73 rdf:type schema:PropertyValue
    74 N4dce14b3e9534a9897c18dd32a4ee72f rdf:first sg:person.01144310126.10
    75 rdf:rest rdf:nil
    76 N6b60a553cc0349129b53b02e47ccdb39 schema:name Springer Nature - SN SciGraph project
    77 rdf:type schema:Organization
    78 N9787fe34e3284a60bb65a49eccf7ce5a rdf:first sg:person.016055775037.93
    79 rdf:rest N05d77ad851034b21950589616eb0b51c
    80 N9c7d2bfd198c4edb8c40d8ef1e66088c schema:volumeNumber 12
    81 rdf:type schema:PublicationVolume
    82 Nb8f5d43b0a0445b588e161d8aa24ef82 schema:name readcube_id
    83 schema:value 6a84f0ebde355d3bfe83dc9e76facb44ae8d36e84ab9b499251019b4b3d04e15
    84 rdf:type schema:PropertyValue
    85 Ndaa4eca69dcf49dc8ccec119cbfb96ce schema:issueNumber 1
    86 rdf:type schema:PublicationIssue
    87 Nf49a3e8a791d4adf8a0cd6ffc749676a rdf:first sg:person.014613330015.13
    88 rdf:rest N4dce14b3e9534a9897c18dd32a4ee72f
    89 Nfb2a5d038e9446cd97c0df272747b3fe rdf:first sg:person.07356564737.39
    90 rdf:rest Nf49a3e8a791d4adf8a0cd6ffc749676a
    91 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    92 schema:name Biological Sciences
    93 rdf:type schema:DefinedTerm
    94 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    95 schema:name Genetics
    96 rdf:type schema:DefinedTerm
    97 sg:grant.5015126 http://pending.schema.org/fundedItem sg:pub.10.1186/s13065-018-0480-8
    98 rdf:type schema:MonetaryGrant
    99 sg:journal.1039133 schema:issn 1864-6158
    100 2191-2858
    101 schema:name Chemistry Central Journal
    102 rdf:type schema:Periodical
    103 sg:person.01144310126.10 schema:affiliation https://www.grid.ac/institutes/grid.412514.7
    104 schema:familyName Liu
    105 schema:givenName Kehai
    106 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144310126.10
    107 rdf:type schema:Person
    108 sg:person.014463034037.52 schema:affiliation https://www.grid.ac/institutes/grid.412514.7
    109 schema:familyName Wu
    110 schema:givenName Junwen
    111 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014463034037.52
    112 rdf:type schema:Person
    113 sg:person.014613330015.13 schema:affiliation https://www.grid.ac/institutes/grid.412514.7
    114 schema:familyName Shu
    115 schema:givenName Hongmei
    116 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014613330015.13
    117 rdf:type schema:Person
    118 sg:person.016055775037.93 schema:affiliation https://www.grid.ac/institutes/grid.412514.7
    119 schema:familyName Zhang
    120 schema:givenName Min
    121 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016055775037.93
    122 rdf:type schema:Person
    123 sg:person.07356564737.39 schema:affiliation https://www.grid.ac/institutes/grid.412514.7
    124 schema:familyName Han
    125 schema:givenName Juanjuan
    126 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07356564737.39
    127 rdf:type schema:Person
    128 sg:pub.10.1007/s00005-013-0243-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043410833
    129 https://doi.org/10.1007/s00005-013-0243-0
    130 rdf:type schema:CreativeWork
    131 sg:pub.10.1007/s00253-011-3829-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025736393
    132 https://doi.org/10.1007/s00253-011-3829-7
    133 rdf:type schema:CreativeWork
    134 sg:pub.10.1007/s11427-012-4419-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003350962
    135 https://doi.org/10.1007/s11427-012-4419-3
    136 rdf:type schema:CreativeWork
    137 sg:pub.10.1007/s11557-011-0754-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006656578
    138 https://doi.org/10.1007/s11557-011-0754-1
    139 rdf:type schema:CreativeWork
    140 sg:pub.10.1038/nature09121 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028162986
    141 https://doi.org/10.1038/nature09121
    142 rdf:type schema:CreativeWork
    143 sg:pub.10.1186/s13065-017-0349-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092827141
    144 https://doi.org/10.1186/s13065-017-0349-2
    145 rdf:type schema:CreativeWork
    146 sg:pub.10.1186/s13065-018-0468-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107224898
    147 https://doi.org/10.1186/s13065-018-0468-4
    148 rdf:type schema:CreativeWork
    149 https://app.dimensions.ai/details/publication/pub.1078432181 schema:CreativeWork
    150 https://doi.org/10.1016/j.bcdf.2014.12.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009364874
    151 rdf:type schema:CreativeWork
    152 https://doi.org/10.1016/j.bcdf.2014.12.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011769883
    153 rdf:type schema:CreativeWork
    154 https://doi.org/10.1016/j.carbpol.2015.05.079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030269541
    155 rdf:type schema:CreativeWork
    156 https://doi.org/10.1016/j.foodres.2015.01.035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007126181
    157 rdf:type schema:CreativeWork
    158 https://doi.org/10.1016/j.ijbiomac.2015.06.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042201079
    159 rdf:type schema:CreativeWork
    160 https://doi.org/10.1016/j.ijbiomac.2015.11.038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043994437
    161 rdf:type schema:CreativeWork
    162 https://doi.org/10.1016/j.ijbiomac.2016.04.067 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049767504
    163 rdf:type schema:CreativeWork
    164 https://doi.org/10.1016/j.intimp.2014.08.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051163784
    165 rdf:type schema:CreativeWork
    166 https://doi.org/10.1016/j.intimp.2014.08.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013267497
    167 rdf:type schema:CreativeWork
    168 https://doi.org/10.1016/j.intimp.2016.08.026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044758320
    169 rdf:type schema:CreativeWork
    170 https://doi.org/10.1016/j.jep.2008.11.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033462545
    171 rdf:type schema:CreativeWork
    172 https://doi.org/10.1016/j.jep.2017.08.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090954000
    173 rdf:type schema:CreativeWork
    174 https://doi.org/10.1016/j.jff.2011.12.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016712715
    175 rdf:type schema:CreativeWork
    176 https://doi.org/10.1016/j.molimm.2014.06.033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019799403
    177 rdf:type schema:CreativeWork
    178 https://doi.org/10.1021/acs.jafc.5b05180 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055095633
    179 rdf:type schema:CreativeWork
    180 https://doi.org/10.1039/c0fo00113a schema:sameAs https://app.dimensions.ai/details/publication/pub.1030195397
    181 rdf:type schema:CreativeWork
    182 https://doi.org/10.1074/jbc.m007386200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037409651
    183 rdf:type schema:CreativeWork
    184 https://doi.org/10.1093/intimm/dxh186 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009264145
    185 rdf:type schema:CreativeWork
    186 https://doi.org/10.1097/shk.0000000000000042 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039807186
    187 rdf:type schema:CreativeWork
    188 https://doi.org/10.1124/pr.109.001073 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034974999
    189 rdf:type schema:CreativeWork
    190 https://doi.org/10.1177/1934578x1100600627 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078432181
    191 rdf:type schema:CreativeWork
    192 https://doi.org/10.3390/md15120388 schema:sameAs https://app.dimensions.ai/details/publication/pub.1099831001
    193 rdf:type schema:CreativeWork
    194 https://doi.org/10.4268/cjcmm20140508 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072428446
    195 rdf:type schema:CreativeWork
    196 https://www.grid.ac/institutes/grid.412514.7 schema:alternateName Shanghai Ocean University
    197 schema:name Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, 999 Hucheng Ring Road, Lingang New City, 201306, Shanghai, China
    198 National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, 201306, Shanghai, China
    199 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...