Evolution of an archaeal virus nucleocapsid protein from the CRISPR-associated Cas4 nuclease View Full Text


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Article Info

DATE

2015-10-29

AUTHORS

Mart Krupovic, Virginija Cvirkaite-Krupovic, David Prangishvili, Eugene V. Koonin

ABSTRACT

Many proteins of viruses infecting hyperthermophilic Crenarchaeota have no detectable homologs in current databases, hampering our understanding of viral evolution. We used sensitive database search methods and structural modeling to show that a nucleocapsid protein (TP1) of Thermoproteus tenax virus 1 (TTV1) is a derivative of the Cas4 nuclease, a component of the CRISPR-Cas adaptive immunity system that is encoded also by several archaeal viruses. In TTV1, the Cas4 gene was split into two, with the N-terminal portion becoming TP1, and lost some of the catalytic amino acid residues, apparently resulting in the inactivation of the nuclease. To our knowledge, this is the first described case of exaptation of an enzyme for a virus capsid protein function. More... »

PAGES

65

References to SciGraph publications

  • 1989-05. Identification and characterization of the genes encoding three structural proteins of the Thermoproteus tenax virus TTV1 in MOLECULAR GENETICS AND GENOMICS
  • 2011-07-14. Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems in BIOLOGY DIRECT
  • 2008-03-03. Redefining viruses: lessons from Mimivirus in NATURE REVIEWS MICROBIOLOGY
  • 2011-05-09. Evolution and classification of the CRISPR–Cas systems in NATURE REVIEWS MICROBIOLOGY
  • 2014-11-06. Chordopoxvirus protein F12 implicated in enveloped virion morphogenesis is an inactivated DNA polymerase in BIOLOGY DIRECT
  • 2014-05-19. Casposons: a new superfamily of self-synthesizing DNA transposons at the origin of prokaryotic CRISPR-Cas immunity in BMC BIOLOGY
  • 1983-10. TTV1, TTV2 and TTV3, a family of viruses of the extremely thermophilic, anaerobic, sulfur reducing archaebacterium Thermoproteus tenax in MOLECULAR GENETICS AND GENOMICS
  • 2011-06-11. Characterization of Sulfolobus islandicus rod-shaped virus 2 gp19, a single-strand specific endonuclease in EXTREMOPHILES
  • 2013-08-23. PROMALS3D: Multiple Protein Sequence Alignment Enhanced with Evolutionary and Three-Dimensional Structural Information in MULTIPLE SEQUENCE ALIGNMENT METHODS
  • 2014-04-29. Conservation of major and minor jelly-roll capsid proteins in Polinton (Maverick) transposons suggests that they are bona fide viruses in BIOLOGY DIRECT
  • 2014-12-09. Evolution of adaptive immunity from transposable elements combined with innate immune systems in NATURE REVIEWS GENETICS
  • 2006-09-19. The ancient Virus World and evolution of cells in BIOLOGY DIRECT
  • 2012-01-22. A new proposed taxon for double-stranded DNA viruses, the order “Ligamenvirales” in ARCHIVES OF VIROLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13062-015-0093-2

    DOI

    http://dx.doi.org/10.1186/s13062-015-0093-2

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1015978589

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26514828


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