Single-cell analysis of CD4+ T-cell differentiation reveals three major cell states and progressive acceleration of proliferation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-12

AUTHORS

Valentina Proserpio, Andrea Piccolo, Liora Haim-Vilmovsky, Gozde Kar, Tapio Lönnberg, Valentine Svensson, Jhuma Pramanik, Kedar Nath Natarajan, Weichao Zhai, Xiuwei Zhang, Giacomo Donati, Melis Kayikci, Jurij Kotar, Andrew N. J. McKenzie, Ruddy Montandon, Oliver Billker, Steven Woodhouse, Pietro Cicuta, Mario Nicodemi, Sarah A. Teichmann

ABSTRACT

BACKGROUND: Differentiation of lymphocytes is frequently accompanied by cell cycle changes, interplay that is of central importance for immunity but is still incompletely understood. Here, we interrogate and quantitatively model how proliferation is linked to differentiation in CD4+ T cells. RESULTS: We perform ex vivo single-cell RNA-sequencing of CD4+ T cells during a mouse model of infection that elicits a type 2 immune response and infer that the differentiated, cytokine-producing cells cycle faster than early activated precursor cells. To dissect this phenomenon quantitatively, we determine expression profiles across consecutive generations of differentiated and undifferentiated cells during Th2 polarization in vitro. We predict three discrete cell states, which we verify by single-cell quantitative PCR. Based on these three states, we extract rates of death, division and differentiation with a branching state Markov model to describe the cell population dynamics. From this multi-scale modelling, we infer a significant acceleration in proliferation from the intermediate activated cell state to the mature cytokine-secreting effector state. We confirm this acceleration both by live imaging of single Th2 cells and in an ex vivo Th1 malaria model by single-cell RNA-sequencing. CONCLUSION: The link between cytokine secretion and proliferation rate holds both in Th1 and Th2 cells in vivo and in vitro, indicating that this is likely a general phenomenon in adaptive immunity. More... »

PAGES

103

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13059-016-0957-5

DOI

http://dx.doi.org/10.1186/s13059-016-0957-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002364913

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27176874


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CD4-Positive T-Lymphocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Proliferation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytokines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Profiling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Malaria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred C57BL", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Biological", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, RNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Single-Cell Analysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcriptome", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "EMBL, European Bioinformatics Institute (EBI), CB10 1SD, Hinxton, UK", 
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Proserpio", 
        "givenName": "Valentina", 
        "id": "sg:person.0675712501.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675712501.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Naples Federico II", 
          "id": "https://www.grid.ac/institutes/grid.4691.a", 
          "name": [
            "Department of Physics, University of Naples Federico II, CNR-Spin, Istituto Nazionale di Fisica Nucleare (INFN), Napoli, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Piccolo", 
        "givenName": "Andrea", 
        "id": "sg:person.0773644373.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0773644373.64"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "EMBL, European Bioinformatics Institute (EBI), CB10 1SD, Hinxton, UK", 
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Haim-Vilmovsky", 
        "givenName": "Liora", 
        "id": "sg:person.01346450542.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346450542.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "European Bioinformatics Institute", 
          "id": "https://www.grid.ac/institutes/grid.225360.0", 
          "name": [
            "EMBL, European Bioinformatics Institute (EBI), CB10 1SD, Hinxton, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kar", 
        "givenName": "Gozde", 
        "id": "sg:person.0755676252.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755676252.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "EMBL, European Bioinformatics Institute (EBI), CB10 1SD, Hinxton, UK", 
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "L\u00f6nnberg", 
        "givenName": "Tapio", 
        "id": "sg:person.01102667320.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102667320.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "European Bioinformatics Institute", 
          "id": "https://www.grid.ac/institutes/grid.225360.0", 
          "name": [
            "EMBL, European Bioinformatics Institute (EBI), CB10 1SD, Hinxton, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Svensson", 
        "givenName": "Valentine", 
        "id": "sg:person.01064247605.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064247605.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "EMBL, European Bioinformatics Institute (EBI), CB10 1SD, Hinxton, UK", 
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pramanik", 
        "givenName": "Jhuma", 
        "id": "sg:person.01272434573.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272434573.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "EMBL, European Bioinformatics Institute (EBI), CB10 1SD, Hinxton, UK", 
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Natarajan", 
        "givenName": "Kedar Nath", 
        "id": "sg:person.01341727415.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01341727415.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Cambridge", 
          "id": "https://www.grid.ac/institutes/grid.5335.0", 
          "name": [
            "Cavendish Laboratory, University of Cambridge, Madingley Road, CB3 0HE, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhai", 
        "givenName": "Weichao", 
        "id": "sg:person.0615143673.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615143673.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "European Bioinformatics Institute", 
          "id": "https://www.grid.ac/institutes/grid.225360.0", 
          "name": [
            "EMBL, European Bioinformatics Institute (EBI), CB10 1SD, Hinxton, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Xiuwei", 
        "id": "sg:person.0614104053.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614104053.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "King's College London", 
          "id": "https://www.grid.ac/institutes/grid.13097.3c", 
          "name": [
            "Centre for Stem Cells and Regenerative Medicine, Kings College London, SE1 9RT, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Donati", 
        "givenName": "Giacomo", 
        "id": "sg:person.0731372273.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0731372273.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "MRC Laboratory of Molecular Biology", 
          "id": "https://www.grid.ac/institutes/grid.42475.30", 
          "name": [
            "MRC Laboratory of Molecular Biology, CB2 0QH, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kayikci", 
        "givenName": "Melis", 
        "id": "sg:person.01021142577.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021142577.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Cambridge", 
          "id": "https://www.grid.ac/institutes/grid.5335.0", 
          "name": [
            "Cavendish Laboratory, University of Cambridge, Madingley Road, CB3 0HE, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kotar", 
        "givenName": "Jurij", 
        "id": "sg:person.01226730661.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01226730661.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "MRC Laboratory of Molecular Biology", 
          "id": "https://www.grid.ac/institutes/grid.42475.30", 
          "name": [
            "MRC Laboratory of Molecular Biology, CB2 0QH, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "McKenzie", 
        "givenName": "Andrew N. J.", 
        "id": "sg:person.0603056221.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603056221.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Montandon", 
        "givenName": "Ruddy", 
        "id": "sg:person.01173340505.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173340505.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Billker", 
        "givenName": "Oliver", 
        "id": "sg:person.01110401146.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110401146.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome / MRC Cambridge Stem Cell Institute", 
          "id": "https://www.grid.ac/institutes/grid.449973.4", 
          "name": [
            "Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge Biomedical Campus, Wellcome Trust/MRC Building, Hills Road, CB2 0XY, Cambridge, UK", 
            "Wellcome Trust\u2014Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Woodhouse", 
        "givenName": "Steven", 
        "id": "sg:person.01166423115.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01166423115.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Cambridge", 
          "id": "https://www.grid.ac/institutes/grid.5335.0", 
          "name": [
            "Cavendish Laboratory, University of Cambridge, Madingley Road, CB3 0HE, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cicuta", 
        "givenName": "Pietro", 
        "id": "sg:person.0654367200.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654367200.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Naples Federico II", 
          "id": "https://www.grid.ac/institutes/grid.4691.a", 
          "name": [
            "Department of Physics, University of Naples Federico II, CNR-Spin, Istituto Nazionale di Fisica Nucleare (INFN), Napoli, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nicodemi", 
        "givenName": "Mario", 
        "id": "sg:person.0714371637.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714371637.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Sanger Institute", 
          "id": "https://www.grid.ac/institutes/grid.10306.34", 
          "name": [
            "EMBL, European Bioinformatics Institute (EBI), CB10 1SD, Hinxton, UK", 
            "Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Teichmann", 
        "givenName": "Sarah A.", 
        "id": "sg:person.01122476621.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01122476621.52"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.tcb.2012.05.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002989280"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.1100179", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003782593"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11538-006-9094-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004143499", 
          "https://doi.org/10.1007/s11538-006-9094-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.20091548", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005314254"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.3154", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007848623", 
          "https://doi.org/10.1038/nbt.3154"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1074-7613(00)80605-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009513720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-immunol-030409-101212", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011226569"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.immunol.17.1.701", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018875055"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0066468", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019639004"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81299-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020092418"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btq057", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025260149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.imbio.2010.12.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027427218"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/stem.5530120502", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028656409"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/stem.5530120502", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028656409"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2015.09.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029349367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2010-11-10-r106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031289083", 
          "https://doi.org/10.1186/gb-2010-11-10-r106"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.16.9488", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037238177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.16.9488", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037238177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.180.5.1955", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038785599"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature13173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042638061", 
          "https://doi.org/10.1038/nature13173"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni.2842", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044237221", 
          "https://doi.org/10.1038/ni.2842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/328626a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044515847", 
          "https://doi.org/10.1038/328626a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pbiomolbio.2004.02.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046650723"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1254257", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046815039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13062-015-0045-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046890157", 
          "https://doi.org/10.1186/s13062-015-0045-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13062-015-0045-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046890157", 
          "https://doi.org/10.1186/s13062-015-0045-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/abbs/gmp018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047238513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/abbs/gmp018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047238513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/excr.1998.4165", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047344023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0015423", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048081110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08900", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048663015", 
          "https://doi.org/10.1038/nature08900"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08900", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048663015", 
          "https://doi.org/10.1038/nature08900"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/07853890310009999", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050819040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/annotation/38e16278-983f-4cc0-a3b6-b16d078a5d70", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1065267278"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1615/critrevimmunol.v18.i3.10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1068131672"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.164.7.3506", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074607358"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.179.10.6485", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077511688"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.181.5.3346", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077692812"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.181.9.6125", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077798845"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-12", 
    "datePublishedReg": "2016-12-01", 
    "description": "BACKGROUND: Differentiation of lymphocytes is frequently accompanied by cell cycle changes, interplay that is of central importance for immunity but is still incompletely understood. Here, we interrogate and quantitatively model how proliferation is linked to differentiation in CD4+ T cells.\nRESULTS: We perform ex vivo single-cell RNA-sequencing of CD4+ T cells during a mouse model of infection that elicits a type 2 immune response and infer that the differentiated, cytokine-producing cells cycle faster than early activated precursor cells. To dissect this phenomenon quantitatively, we determine expression profiles across consecutive generations of differentiated and undifferentiated cells during Th2 polarization in vitro. We predict three discrete cell states, which we verify by single-cell quantitative PCR. Based on these three states, we extract rates of death, division and differentiation with a branching state Markov model to describe the cell population dynamics. From this multi-scale modelling, we infer a significant acceleration in proliferation from the intermediate activated cell state to the mature cytokine-secreting effector state. We confirm this acceleration both by live imaging of single Th2 cells and in an ex vivo Th1 malaria model by single-cell RNA-sequencing.\nCONCLUSION: The link between cytokine secretion and proliferation rate holds both in Th1 and Th2 cells in vivo and in vitro, indicating that this is likely a general phenomenon in adaptive immunity.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s13059-016-0957-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2753701", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5212449", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5236522", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.4455054", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2779972", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3782946", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3640455", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1023439", 
        "issn": [
          "1474-760X", 
          "1465-6906"
        ], 
        "name": "Genome Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "17"
      }
    ], 
    "name": "Single-cell analysis of CD4+ T-cell differentiation reveals three major cell states and progressive acceleration of proliferation", 
    "pagination": "103", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "0b4294509519521d53bada4e5e46367941be577b837cc112c759d012b9c9f824"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27176874"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100960660"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s13059-016-0957-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1002364913"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s13059-016-0957-5", 
      "https://app.dimensions.ai/details/publication/pub.1002364913"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:53", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000549.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2Fs13059-016-0957-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13059-016-0957-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13059-016-0957-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13059-016-0957-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13059-016-0957-5'


 

This table displays all metadata directly associated to this object as RDF triples.

406 TRIPLES      21 PREDICATES      78 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s13059-016-0957-5 schema:about N074dd7b79f98415ca3f5979d63ec932e
2 N1278dfde735f47e9a4e05ae6c983682a
3 N5881972af7064dfb8185fbb87b20e9e4
4 N6aad8b71fae040aa9250f214a849193f
5 N8445e4d362324c72afce44e12d6d13de
6 N870e06657aaf487ca1a774f227a2ed55
7 N8b5a2a8a76644c33a62697ac96e6c620
8 N925e2f34298d45c59bbef8bd21987de9
9 N9dfa1e8a26f44052bc04e5e2230308d2
10 N9f872eb4190a4a26bd9cc83c3f6ba2f4
11 N9ff3e59d814341e8a6a349afe6649028
12 Nba057f2c34694b168f29533abbb0baeb
13 Nca347c3b4be04f8da54e467d84fc676d
14 Nee1ee7ebee094682955a6f81d67def86
15 Nf4fb28ac00024c14a4e51d21a2fbf72a
16 anzsrc-for:11
17 anzsrc-for:1107
18 schema:author N768befc22d9640629d3a7f093b8d9fd8
19 schema:citation sg:pub.10.1007/s11538-006-9094-8
20 sg:pub.10.1038/328626a0
21 sg:pub.10.1038/nature08900
22 sg:pub.10.1038/nature13173
23 sg:pub.10.1038/nbt.3154
24 sg:pub.10.1038/ni.2842
25 sg:pub.10.1186/gb-2010-11-10-r106
26 sg:pub.10.1186/s13062-015-0045-x
27 https://doi.org/10.1002/stem.5530120502
28 https://doi.org/10.1006/excr.1998.4165
29 https://doi.org/10.1016/j.imbio.2010.12.003
30 https://doi.org/10.1016/j.pbiomolbio.2004.02.007
31 https://doi.org/10.1016/j.stem.2015.09.011
32 https://doi.org/10.1016/j.tcb.2012.05.004
33 https://doi.org/10.1016/s0092-8674(00)81299-4
34 https://doi.org/10.1016/s1074-7613(00)80605-6
35 https://doi.org/10.1073/pnas.95.16.9488
36 https://doi.org/10.1080/07853890310009999
37 https://doi.org/10.1084/jem.180.5.1955
38 https://doi.org/10.1084/jem.20091548
39 https://doi.org/10.1093/abbs/gmp018
40 https://doi.org/10.1093/bioinformatics/btq057
41 https://doi.org/10.1126/science.1254257
42 https://doi.org/10.1146/annurev-immunol-030409-101212
43 https://doi.org/10.1146/annurev.immunol.17.1.701
44 https://doi.org/10.1371/annotation/38e16278-983f-4cc0-a3b6-b16d078a5d70
45 https://doi.org/10.1371/journal.pone.0015423
46 https://doi.org/10.1371/journal.pone.0066468
47 https://doi.org/10.1615/critrevimmunol.v18.i3.10
48 https://doi.org/10.4049/jimmunol.1100179
49 https://doi.org/10.4049/jimmunol.164.7.3506
50 https://doi.org/10.4049/jimmunol.179.10.6485
51 https://doi.org/10.4049/jimmunol.181.5.3346
52 https://doi.org/10.4049/jimmunol.181.9.6125
53 schema:datePublished 2016-12
54 schema:datePublishedReg 2016-12-01
55 schema:description BACKGROUND: Differentiation of lymphocytes is frequently accompanied by cell cycle changes, interplay that is of central importance for immunity but is still incompletely understood. Here, we interrogate and quantitatively model how proliferation is linked to differentiation in CD4+ T cells. RESULTS: We perform ex vivo single-cell RNA-sequencing of CD4+ T cells during a mouse model of infection that elicits a type 2 immune response and infer that the differentiated, cytokine-producing cells cycle faster than early activated precursor cells. To dissect this phenomenon quantitatively, we determine expression profiles across consecutive generations of differentiated and undifferentiated cells during Th2 polarization in vitro. We predict three discrete cell states, which we verify by single-cell quantitative PCR. Based on these three states, we extract rates of death, division and differentiation with a branching state Markov model to describe the cell population dynamics. From this multi-scale modelling, we infer a significant acceleration in proliferation from the intermediate activated cell state to the mature cytokine-secreting effector state. We confirm this acceleration both by live imaging of single Th2 cells and in an ex vivo Th1 malaria model by single-cell RNA-sequencing. CONCLUSION: The link between cytokine secretion and proliferation rate holds both in Th1 and Th2 cells in vivo and in vitro, indicating that this is likely a general phenomenon in adaptive immunity.
56 schema:genre research_article
57 schema:inLanguage en
58 schema:isAccessibleForFree true
59 schema:isPartOf N083fcede3fbd45ce887ca137c04b07a1
60 N0e60fcfa1bf2490698f170ece0c08905
61 sg:journal.1023439
62 schema:name Single-cell analysis of CD4+ T-cell differentiation reveals three major cell states and progressive acceleration of proliferation
63 schema:pagination 103
64 schema:productId N194641e18ddc42e9be74020f61cf3725
65 N9dabfd62e51d4a05b85fe753d6cd1dd0
66 Naff1e9a3cc31406894a10639541fc087
67 Nd17afb10171f438b85629221eed31d6c
68 Ne528b8e84f8346fab0529ae2c048c25e
69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002364913
70 https://doi.org/10.1186/s13059-016-0957-5
71 schema:sdDatePublished 2019-04-10T20:53
72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
73 schema:sdPublisher N736c96f50bee47329f43b307030f2bda
74 schema:url http://link.springer.com/10.1186%2Fs13059-016-0957-5
75 sgo:license sg:explorer/license/
76 sgo:sdDataset articles
77 rdf:type schema:ScholarlyArticle
78 N02b834779a484e8c8a00a30defd4fabd rdf:first sg:person.01064247605.85
79 rdf:rest N96c086c57b35487c97cb1522e63a5d36
80 N074dd7b79f98415ca3f5979d63ec932e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Cytokines
82 rdf:type schema:DefinedTerm
83 N083fcede3fbd45ce887ca137c04b07a1 schema:volumeNumber 17
84 rdf:type schema:PublicationVolume
85 N0e60fcfa1bf2490698f170ece0c08905 schema:issueNumber 1
86 rdf:type schema:PublicationIssue
87 N1278dfde735f47e9a4e05ae6c983682a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Models, Biological
89 rdf:type schema:DefinedTerm
90 N194641e18ddc42e9be74020f61cf3725 schema:name readcube_id
91 schema:value 0b4294509519521d53bada4e5e46367941be577b837cc112c759d012b9c9f824
92 rdf:type schema:PropertyValue
93 N31b2e14a27b04bf891b7fdc750be33da rdf:first sg:person.01173340505.39
94 rdf:rest Ne56e35dc4c1f4a81a9422ee2edca829c
95 N342385135f114f1c9c1a6dcd20262d8c rdf:first sg:person.01122476621.52
96 rdf:rest rdf:nil
97 N4193e777ffb546048941aeab768caab6 rdf:first sg:person.0614104053.75
98 rdf:rest Nda0ab0fe9fa4482fa7acdc7da75d1ecd
99 N4aa20209a36444bfbe8f2cfd5a84b45f rdf:first sg:person.01021142577.23
100 rdf:rest Ne36cb9008a724b85a0efd312c80dd292
101 N518cfc393632455cad255e7955144b65 rdf:first sg:person.0773644373.64
102 rdf:rest Nf2208f41de424322932bf69a916c4823
103 N585dc5ec54f24e6ebb54543c3fab1422 rdf:first sg:person.0654367200.21
104 rdf:rest Nafbaa0b9ac744204b1f3231022e718ee
105 N5881972af7064dfb8185fbb87b20e9e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Sequence Analysis, RNA
107 rdf:type schema:DefinedTerm
108 N68293ee3847e40d2b37c2d71c07797b1 rdf:first sg:person.0603056221.77
109 rdf:rest N31b2e14a27b04bf891b7fdc750be33da
110 N6aad8b71fae040aa9250f214a849193f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Cells, Cultured
112 rdf:type schema:DefinedTerm
113 N736c96f50bee47329f43b307030f2bda schema:name Springer Nature - SN SciGraph project
114 rdf:type schema:Organization
115 N768befc22d9640629d3a7f093b8d9fd8 rdf:first sg:person.0675712501.33
116 rdf:rest N518cfc393632455cad255e7955144b65
117 N7fe660f8ca4a471e909cb02aa24c4f71 rdf:first sg:person.0755676252.02
118 rdf:rest Nb8f58fa762b642ee82af5e1162aa25e2
119 N8445e4d362324c72afce44e12d6d13de schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Single-Cell Analysis
121 rdf:type schema:DefinedTerm
122 N870e06657aaf487ca1a774f227a2ed55 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name CD4-Positive T-Lymphocytes
124 rdf:type schema:DefinedTerm
125 N89d1491a3aba4d6086eff44619107e4d rdf:first sg:person.01341727415.33
126 rdf:rest Nc66aad53f7a64338b98fbf57e5b8d72c
127 N8b5a2a8a76644c33a62697ac96e6c620 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Cell Differentiation
129 rdf:type schema:DefinedTerm
130 N925e2f34298d45c59bbef8bd21987de9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Malaria
132 rdf:type schema:DefinedTerm
133 N96c086c57b35487c97cb1522e63a5d36 rdf:first sg:person.01272434573.97
134 rdf:rest N89d1491a3aba4d6086eff44619107e4d
135 N9dabfd62e51d4a05b85fe753d6cd1dd0 schema:name nlm_unique_id
136 schema:value 100960660
137 rdf:type schema:PropertyValue
138 N9dfa1e8a26f44052bc04e5e2230308d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Transcriptome
140 rdf:type schema:DefinedTerm
141 N9f872eb4190a4a26bd9cc83c3f6ba2f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Animals
143 rdf:type schema:DefinedTerm
144 N9ff3e59d814341e8a6a349afe6649028 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Gene Expression Profiling
146 rdf:type schema:DefinedTerm
147 Nafbaa0b9ac744204b1f3231022e718ee rdf:first sg:person.0714371637.39
148 rdf:rest N342385135f114f1c9c1a6dcd20262d8c
149 Naff1e9a3cc31406894a10639541fc087 schema:name dimensions_id
150 schema:value pub.1002364913
151 rdf:type schema:PropertyValue
152 Nb8f58fa762b642ee82af5e1162aa25e2 rdf:first sg:person.01102667320.32
153 rdf:rest N02b834779a484e8c8a00a30defd4fabd
154 Nba057f2c34694b168f29533abbb0baeb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Mice
156 rdf:type schema:DefinedTerm
157 Nc66aad53f7a64338b98fbf57e5b8d72c rdf:first sg:person.0615143673.72
158 rdf:rest N4193e777ffb546048941aeab768caab6
159 Nc9d3a6fb33eb43d49c95fe5eefa9765c rdf:first sg:person.01166423115.58
160 rdf:rest N585dc5ec54f24e6ebb54543c3fab1422
161 Nca347c3b4be04f8da54e467d84fc676d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Female
163 rdf:type schema:DefinedTerm
164 Nd17afb10171f438b85629221eed31d6c schema:name pubmed_id
165 schema:value 27176874
166 rdf:type schema:PropertyValue
167 Nda0ab0fe9fa4482fa7acdc7da75d1ecd rdf:first sg:person.0731372273.05
168 rdf:rest N4aa20209a36444bfbe8f2cfd5a84b45f
169 Ne36cb9008a724b85a0efd312c80dd292 rdf:first sg:person.01226730661.05
170 rdf:rest N68293ee3847e40d2b37c2d71c07797b1
171 Ne528b8e84f8346fab0529ae2c048c25e schema:name doi
172 schema:value 10.1186/s13059-016-0957-5
173 rdf:type schema:PropertyValue
174 Ne56e35dc4c1f4a81a9422ee2edca829c rdf:first sg:person.01110401146.53
175 rdf:rest Nc9d3a6fb33eb43d49c95fe5eefa9765c
176 Nee1ee7ebee094682955a6f81d67def86 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
177 schema:name Cell Proliferation
178 rdf:type schema:DefinedTerm
179 Nf2208f41de424322932bf69a916c4823 rdf:first sg:person.01346450542.55
180 rdf:rest N7fe660f8ca4a471e909cb02aa24c4f71
181 Nf4fb28ac00024c14a4e51d21a2fbf72a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
182 schema:name Mice, Inbred C57BL
183 rdf:type schema:DefinedTerm
184 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
185 schema:name Medical and Health Sciences
186 rdf:type schema:DefinedTerm
187 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
188 schema:name Immunology
189 rdf:type schema:DefinedTerm
190 sg:grant.2753701 http://pending.schema.org/fundedItem sg:pub.10.1186/s13059-016-0957-5
191 rdf:type schema:MonetaryGrant
192 sg:grant.2779972 http://pending.schema.org/fundedItem sg:pub.10.1186/s13059-016-0957-5
193 rdf:type schema:MonetaryGrant
194 sg:grant.3640455 http://pending.schema.org/fundedItem sg:pub.10.1186/s13059-016-0957-5
195 rdf:type schema:MonetaryGrant
196 sg:grant.3782946 http://pending.schema.org/fundedItem sg:pub.10.1186/s13059-016-0957-5
197 rdf:type schema:MonetaryGrant
198 sg:grant.4455054 http://pending.schema.org/fundedItem sg:pub.10.1186/s13059-016-0957-5
199 rdf:type schema:MonetaryGrant
200 sg:grant.5212449 http://pending.schema.org/fundedItem sg:pub.10.1186/s13059-016-0957-5
201 rdf:type schema:MonetaryGrant
202 sg:grant.5236522 http://pending.schema.org/fundedItem sg:pub.10.1186/s13059-016-0957-5
203 rdf:type schema:MonetaryGrant
204 sg:journal.1023439 schema:issn 1465-6906
205 1474-760X
206 schema:name Genome Biology
207 rdf:type schema:Periodical
208 sg:person.01021142577.23 schema:affiliation https://www.grid.ac/institutes/grid.42475.30
209 schema:familyName Kayikci
210 schema:givenName Melis
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021142577.23
212 rdf:type schema:Person
213 sg:person.01064247605.85 schema:affiliation https://www.grid.ac/institutes/grid.225360.0
214 schema:familyName Svensson
215 schema:givenName Valentine
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064247605.85
217 rdf:type schema:Person
218 sg:person.01102667320.32 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
219 schema:familyName Lönnberg
220 schema:givenName Tapio
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102667320.32
222 rdf:type schema:Person
223 sg:person.01110401146.53 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
224 schema:familyName Billker
225 schema:givenName Oliver
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110401146.53
227 rdf:type schema:Person
228 sg:person.01122476621.52 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
229 schema:familyName Teichmann
230 schema:givenName Sarah A.
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01122476621.52
232 rdf:type schema:Person
233 sg:person.01166423115.58 schema:affiliation https://www.grid.ac/institutes/grid.449973.4
234 schema:familyName Woodhouse
235 schema:givenName Steven
236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01166423115.58
237 rdf:type schema:Person
238 sg:person.01173340505.39 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
239 schema:familyName Montandon
240 schema:givenName Ruddy
241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173340505.39
242 rdf:type schema:Person
243 sg:person.01226730661.05 schema:affiliation https://www.grid.ac/institutes/grid.5335.0
244 schema:familyName Kotar
245 schema:givenName Jurij
246 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01226730661.05
247 rdf:type schema:Person
248 sg:person.01272434573.97 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
249 schema:familyName Pramanik
250 schema:givenName Jhuma
251 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272434573.97
252 rdf:type schema:Person
253 sg:person.01341727415.33 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
254 schema:familyName Natarajan
255 schema:givenName Kedar Nath
256 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01341727415.33
257 rdf:type schema:Person
258 sg:person.01346450542.55 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
259 schema:familyName Haim-Vilmovsky
260 schema:givenName Liora
261 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346450542.55
262 rdf:type schema:Person
263 sg:person.0603056221.77 schema:affiliation https://www.grid.ac/institutes/grid.42475.30
264 schema:familyName McKenzie
265 schema:givenName Andrew N. J.
266 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603056221.77
267 rdf:type schema:Person
268 sg:person.0614104053.75 schema:affiliation https://www.grid.ac/institutes/grid.225360.0
269 schema:familyName Zhang
270 schema:givenName Xiuwei
271 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614104053.75
272 rdf:type schema:Person
273 sg:person.0615143673.72 schema:affiliation https://www.grid.ac/institutes/grid.5335.0
274 schema:familyName Zhai
275 schema:givenName Weichao
276 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615143673.72
277 rdf:type schema:Person
278 sg:person.0654367200.21 schema:affiliation https://www.grid.ac/institutes/grid.5335.0
279 schema:familyName Cicuta
280 schema:givenName Pietro
281 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654367200.21
282 rdf:type schema:Person
283 sg:person.0675712501.33 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
284 schema:familyName Proserpio
285 schema:givenName Valentina
286 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675712501.33
287 rdf:type schema:Person
288 sg:person.0714371637.39 schema:affiliation https://www.grid.ac/institutes/grid.4691.a
289 schema:familyName Nicodemi
290 schema:givenName Mario
291 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714371637.39
292 rdf:type schema:Person
293 sg:person.0731372273.05 schema:affiliation https://www.grid.ac/institutes/grid.13097.3c
294 schema:familyName Donati
295 schema:givenName Giacomo
296 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0731372273.05
297 rdf:type schema:Person
298 sg:person.0755676252.02 schema:affiliation https://www.grid.ac/institutes/grid.225360.0
299 schema:familyName Kar
300 schema:givenName Gozde
301 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755676252.02
302 rdf:type schema:Person
303 sg:person.0773644373.64 schema:affiliation https://www.grid.ac/institutes/grid.4691.a
304 schema:familyName Piccolo
305 schema:givenName Andrea
306 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0773644373.64
307 rdf:type schema:Person
308 sg:pub.10.1007/s11538-006-9094-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004143499
309 https://doi.org/10.1007/s11538-006-9094-8
310 rdf:type schema:CreativeWork
311 sg:pub.10.1038/328626a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044515847
312 https://doi.org/10.1038/328626a0
313 rdf:type schema:CreativeWork
314 sg:pub.10.1038/nature08900 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048663015
315 https://doi.org/10.1038/nature08900
316 rdf:type schema:CreativeWork
317 sg:pub.10.1038/nature13173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042638061
318 https://doi.org/10.1038/nature13173
319 rdf:type schema:CreativeWork
320 sg:pub.10.1038/nbt.3154 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007848623
321 https://doi.org/10.1038/nbt.3154
322 rdf:type schema:CreativeWork
323 sg:pub.10.1038/ni.2842 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044237221
324 https://doi.org/10.1038/ni.2842
325 rdf:type schema:CreativeWork
326 sg:pub.10.1186/gb-2010-11-10-r106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031289083
327 https://doi.org/10.1186/gb-2010-11-10-r106
328 rdf:type schema:CreativeWork
329 sg:pub.10.1186/s13062-015-0045-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1046890157
330 https://doi.org/10.1186/s13062-015-0045-x
331 rdf:type schema:CreativeWork
332 https://doi.org/10.1002/stem.5530120502 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028656409
333 rdf:type schema:CreativeWork
334 https://doi.org/10.1006/excr.1998.4165 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047344023
335 rdf:type schema:CreativeWork
336 https://doi.org/10.1016/j.imbio.2010.12.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027427218
337 rdf:type schema:CreativeWork
338 https://doi.org/10.1016/j.pbiomolbio.2004.02.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046650723
339 rdf:type schema:CreativeWork
340 https://doi.org/10.1016/j.stem.2015.09.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029349367
341 rdf:type schema:CreativeWork
342 https://doi.org/10.1016/j.tcb.2012.05.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002989280
343 rdf:type schema:CreativeWork
344 https://doi.org/10.1016/s0092-8674(00)81299-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020092418
345 rdf:type schema:CreativeWork
346 https://doi.org/10.1016/s1074-7613(00)80605-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009513720
347 rdf:type schema:CreativeWork
348 https://doi.org/10.1073/pnas.95.16.9488 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037238177
349 rdf:type schema:CreativeWork
350 https://doi.org/10.1080/07853890310009999 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050819040
351 rdf:type schema:CreativeWork
352 https://doi.org/10.1084/jem.180.5.1955 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038785599
353 rdf:type schema:CreativeWork
354 https://doi.org/10.1084/jem.20091548 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005314254
355 rdf:type schema:CreativeWork
356 https://doi.org/10.1093/abbs/gmp018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047238513
357 rdf:type schema:CreativeWork
358 https://doi.org/10.1093/bioinformatics/btq057 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025260149
359 rdf:type schema:CreativeWork
360 https://doi.org/10.1126/science.1254257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046815039
361 rdf:type schema:CreativeWork
362 https://doi.org/10.1146/annurev-immunol-030409-101212 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011226569
363 rdf:type schema:CreativeWork
364 https://doi.org/10.1146/annurev.immunol.17.1.701 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018875055
365 rdf:type schema:CreativeWork
366 https://doi.org/10.1371/annotation/38e16278-983f-4cc0-a3b6-b16d078a5d70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1065267278
367 rdf:type schema:CreativeWork
368 https://doi.org/10.1371/journal.pone.0015423 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048081110
369 rdf:type schema:CreativeWork
370 https://doi.org/10.1371/journal.pone.0066468 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019639004
371 rdf:type schema:CreativeWork
372 https://doi.org/10.1615/critrevimmunol.v18.i3.10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068131672
373 rdf:type schema:CreativeWork
374 https://doi.org/10.4049/jimmunol.1100179 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003782593
375 rdf:type schema:CreativeWork
376 https://doi.org/10.4049/jimmunol.164.7.3506 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074607358
377 rdf:type schema:CreativeWork
378 https://doi.org/10.4049/jimmunol.179.10.6485 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077511688
379 rdf:type schema:CreativeWork
380 https://doi.org/10.4049/jimmunol.181.5.3346 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077692812
381 rdf:type schema:CreativeWork
382 https://doi.org/10.4049/jimmunol.181.9.6125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077798845
383 rdf:type schema:CreativeWork
384 https://www.grid.ac/institutes/grid.10306.34 schema:alternateName Wellcome Sanger Institute
385 schema:name EMBL, European Bioinformatics Institute (EBI), CB10 1SD, Hinxton, UK
386 Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Hinxton, Cambridge, UK
387 rdf:type schema:Organization
388 https://www.grid.ac/institutes/grid.13097.3c schema:alternateName King's College London
389 schema:name Centre for Stem Cells and Regenerative Medicine, Kings College London, SE1 9RT, London, UK
390 rdf:type schema:Organization
391 https://www.grid.ac/institutes/grid.225360.0 schema:alternateName European Bioinformatics Institute
392 schema:name EMBL, European Bioinformatics Institute (EBI), CB10 1SD, Hinxton, UK
393 rdf:type schema:Organization
394 https://www.grid.ac/institutes/grid.42475.30 schema:alternateName MRC Laboratory of Molecular Biology
395 schema:name MRC Laboratory of Molecular Biology, CB2 0QH, Cambridge, UK
396 rdf:type schema:Organization
397 https://www.grid.ac/institutes/grid.449973.4 schema:alternateName Wellcome / MRC Cambridge Stem Cell Institute
398 schema:name Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge Biomedical Campus, Wellcome Trust/MRC Building, Hills Road, CB2 0XY, Cambridge, UK
399 Wellcome Trust—Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK
400 rdf:type schema:Organization
401 https://www.grid.ac/institutes/grid.4691.a schema:alternateName University of Naples Federico II
402 schema:name Department of Physics, University of Naples Federico II, CNR-Spin, Istituto Nazionale di Fisica Nucleare (INFN), Napoli, Italy
403 rdf:type schema:Organization
404 https://www.grid.ac/institutes/grid.5335.0 schema:alternateName University of Cambridge
405 schema:name Cavendish Laboratory, University of Cambridge, Madingley Road, CB3 0HE, Cambridge, UK
406 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...