Nck-associated protein 1 associates with HSP90 to drive metastasis in human non-small-cell lung cancer View Full Text


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Article Info

DATE

2019-12

AUTHORS

Yuanping Xiong, Leilei He, Chloe Shay, Liwei Lang, Jenni Loveless, Jieqing Yu, Ron Chemmalakuzhy, Hongqun Jiang, Manran Liu, Yong Teng

ABSTRACT

BACKGROUND: Metastatic lung cancer is a life-threatening condition that develops when cancer in another area of the body metastasizes, or spreads, to the lung. Despite advances in our understanding of primary lung oncogenesis, the biological basis driving the progression from primary to metastatic lung cancer remains poorly characterized. METHODS: Genetic knockdown of the particular genes in cancer cells were achieved by lentiviral-mediated interference. Invasion potential was determined by Matrigel and three-dimensional invasion. The secretion of matrix metalloproteinase 2 (MMP2) and MMP9 were measured by ELISA. Protein levels were assessed by Western blotting and immunohistochemistry. Protein-protein interactions were determined by immunoprecipitation. An experimental mouse model was generated to investigate the gene regulation in tumor growth and metastasis. RESULTS: Nck-associated protein 1 (NAP1/NCKAP1) is highly expressed in primary non-small-cell lung cancer (NSCLC) when compared with adjacent normal lung tissues, and its expression levels are strongly associated with the histologic tumor grade, metastasis and poor survival rate of NSCLC patients. Overexpression of NAP1 in lowly invasive NSCLC cells enhances MMP9 secretion and invasion potential, whereas NAP1 silencing in highly invasive NSCLC cells produces opposing effects in comparison. Mechanistic studies further reveal that the binding of NAP1 to the cellular chaperone heat shock protein 90 (HSP90) is required for its protein stabilization, and NAP1 plays an essential role in HSP90-mediated invasion and metastasis by provoking MMP9 activation and the epithelial-to-mesenchymal transition in NSCLC cells. CONCLUSIONS: Our insights demonstrate the importance and functional regulation of the HSP90-NAP1 protein complex in cancer metastatic signaling, which spur new avenues to target this interaction as a novel approach to block NSCLC metastasis. More... »

PAGES

122

References to SciGraph publications

  • 2016-03. Arpin downregulation in breast cancer is associated with poor prognosis in BRITISH JOURNAL OF CANCER
  • 2010-10. An online survival analysis tool to rapidly assess the effect of 22,277 genes on breast cancer prognosis using microarray data of 1,809 patients in BREAST CANCER RESEARCH AND TREATMENT
  • 2010-07. HSP90 at the hub of protein homeostasis: emerging mechanistic insights in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2017-04-21. The HSP90 chaperone machinery in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2016-01. Mitochondrial ATAD3A combines with GRP78 to regulate the WASF3 metastasis-promoting protein in ONCOGENE
  • 2012-12. Upregulation of Hsp90-beta and annexin A1 correlates with poor survival and lymphatic metastasis in lung cancer patients in JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
  • 2017-02. MicroRNA-34c-3p promotes cell proliferation and invasion in hepatocellular carcinoma by regulation of NCKAP1 expression in JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
  • 2017-11. Expanding the genetic heterogeneity of intellectual disability in HUMAN GENETICS
  • 2016-03. Progression and metastasis of lung cancer in CANCER AND METASTASIS REVIEWS
  • 2008-07. WAVE and Arp2/3 jointly inhibit filopodium formation by entering into a complex with mDia2 in NATURE CELL BIOLOGY
  • 2017-12. Augmentation of the anticancer activity of CYT997 in human prostate cancer by inhibiting Src activity in JOURNAL OF HEMATOLOGY & ONCOLOGY
  • 2017-12. Combined targeting of Arf1 and Ras potentiates anticancer activity for prostate cancer therapeutics in JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
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    http://scigraph.springernature.com/pub.10.1186/s13046-019-1124-0

    DOI

    http://dx.doi.org/10.1186/s13046-019-1124-0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112739188

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30867003


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