Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Wei-Lun Hwang, Hsin-Yi Lan, Wei-Chung Cheng, Shih-Ching Huang, Muh-Hwa Yang

ABSTRACT

BACKGROUND: Cell-cell interactions maintain tissue homeostasis and contribute to dynamic alteration of the tumor microenvironment (TME). Communication between cancer and host cells not only promotes advanced disease aggression but also determines therapeutic response in cancer patients. Despite accumulating evidence supporting the role of tumor-infiltrating immunocytes in modulating tumor immunity, the interplay between heterogeneous tumor subpopulations and immunocytes is elusive. METHODS: We expanded colorectal cancer stem cells (CRCSCs) as cancer spheroids from the murine colorectal cancer (CRC) cell line CT26 to interrogate tumor-host interactions using a syngeneic tumor model. RNA-sequencing analysis of host cells and tumor exosomes was performed to identify molecular determinants that mediate the crosstalk between CRCSCs and immunocytes. The Cancer Genome Atlas (TCGA) database was used to validate the clinical significance in CRC patients. RESULTS: The expanded CT26 cancer spheroids showed increased stemness gene expression, enhanced spheroid and clonogenicity potential, and an elevated tumor-initiating ability, characteristic of CRCSCs. By examining immune cell composition in syngeneic tumor-bearing mice, a systemic increase in CD11b+/Ly6GHigh/Ly6CLow neutrophils was observed in mice bearing CRCSC-derived tumors. An increased secretion of CRCSC exosomes was observed in vitro, and through in vivo tracking, CRCSC exosomes were found to be transported to the bone marrow. Moreover, CRCSC exosomes prolonged the survival of bone marrow-derived neutrophils and engendered a protumoral phenotype in neutrophils. Mechanistically, tumor exosomal tri-phosphate RNAs induced the expression of interleukin-1β (IL-1β) through a pattern recognition-NF-κB signaling axis to sustain neutrophil survival. CRCSC-secreted CXCL1 and CXCL2 then attracted CRCSC-primed neutrophils to promote tumorigenesis of CRC cells via IL-1β. Moreover, neutrophil depletion using a Ly6G-specific antibody (clone 1A8) attenuated the tumorigenicity of CRCSCs. In human specimens, CRC patients exhibiting an active CRCSC signal (Snail+IL8+) showed elevated tumor infiltration of MPO+ neutrophils, and high (in the top 10%) MPO expression predicted poor survival of CRC patients. CONCLUSIONS: This study elucidates a multistep CRCSC-neutrophil interaction during advanced cancer progression. Strategies targeting aberrant neutrophil activation may be developed for combating CSC-related malignancy. More... »

PAGES

10

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13045-019-0699-4

DOI

http://dx.doi.org/10.1186/s13045-019-0699-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111656941

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30683126


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "National Yang Ming University", 
          "id": "https://www.grid.ac/institutes/grid.260770.4", 
          "name": [
            "Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, No. 155, Sec. 2, Li-Nong St., 11221, Taipei, Taiwan", 
            "The Ph.D. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, No.250, Wuxing St., 11031, Taipei, Taiwan", 
            "Cancer Progression Research Center, National Yang-Ming University, No. 155, Sec. 2, Li-Nong Street, 11221, Taipei, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hwang", 
        "givenName": "Wei-Lun", 
        "id": "sg:person.01231257400.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231257400.70"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Yang Ming University", 
          "id": "https://www.grid.ac/institutes/grid.260770.4", 
          "name": [
            "Institute of Clinical Medicine, National Yang-Ming University, No. 155, Sec. 2, Li-Nong Street, 11221, Taipei, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lan", 
        "givenName": "Hsin-Yi", 
        "id": "sg:person.0760206621.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760206621.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "China Medical University", 
          "id": "https://www.grid.ac/institutes/grid.254145.3", 
          "name": [
            "Graduate Institute of Biomedical Science, Research Center for Tumor Medical Science, and Drug Development Center, China Medical University, No.6, Hsueh-Shih Road, 40403, Taichung, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cheng", 
        "givenName": "Wei-Chung", 
        "id": "sg:person.0622547475.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622547475.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Yang Ming University", 
          "id": "https://www.grid.ac/institutes/grid.260770.4", 
          "name": [
            "Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, No. 155, Sec. 2, Li-Nong St., 11221, Taipei, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huang", 
        "givenName": "Shih-Ching", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Yang Ming University", 
          "id": "https://www.grid.ac/institutes/grid.260770.4", 
          "name": [
            "Institute of Clinical Medicine, National Yang-Ming University, No. 155, Sec. 2, Li-Nong Street, 11221, Taipei, Taiwan", 
            "Division of Medical Oncology, Department of Oncology, Taipei Veterans General Hospital, No.201, Sec. 2, Shipai Rd., 11217, Taipei, Taiwan", 
            "Cancer Progression Research Center, National Yang-Ming University, No. 155, Sec. 2, Li-Nong Street, 11221, Taipei, Taiwan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yang", 
        "givenName": "Muh-Hwa", 
        "id": "sg:person.01270363147.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270363147.69"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1172/jci61067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001453830"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb2910", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002357372", 
          "https://doi.org/10.1038/ncb2910"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb2910", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002357372", 
          "https://doi.org/10.1038/ncb2910"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb2910", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002357372", 
          "https://doi.org/10.1038/ncb2910"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri855", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002497020", 
          "https://doi.org/10.1038/nri855"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri855", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002497020", 
          "https://doi.org/10.1038/nri855"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2249.2002.01836.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002645474"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.176.1.284", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007173345"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2011.02.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010215023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-06-3126", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010617336"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/bts587", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012255299"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ccr.2006.03.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013645548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb3346", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014129034", 
          "https://doi.org/10.1038/ncb3346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.it.2016.03.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014821554"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0437939100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018569245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature15756", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018970538", 
          "https://doi.org/10.1038/nature15756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2147/ijn.s13402", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019482319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1132505", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020542785"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07602", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020963310", 
          "https://doi.org/10.1038/nature07602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature14971", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021631986", 
          "https://doi.org/10.1038/nature14971"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.13.7556", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022907036"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2011.02.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023540354"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2010-09-308387", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023718905"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.apsb.2016.02.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024172511"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1189/jlb.0306164", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024353994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ccell.2014.12.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025732101"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/1078-0432.ccr-15-2463", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025747537"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm.2084", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026729604", 
          "https://doi.org/10.1038/nm.2084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm.2084", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026729604", 
          "https://doi.org/10.1038/nm.2084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg1840", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026833222", 
          "https://doi.org/10.1038/nrg1840"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg1840", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026833222", 
          "https://doi.org/10.1038/nrg1840"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood.v98.10.3143", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028513687"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.20040624", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030179768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc.2016.54", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030891341", 
          "https://doi.org/10.1038/nrc.2016.54"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0030806", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032689931"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni1087", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032695282", 
          "https://doi.org/10.1038/ni1087"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni1087", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032695282", 
          "https://doi.org/10.1038/ni1087"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.it.2016.01.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033522829"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.97.2.329", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036056299"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2010/201026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036440340"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3402/jev.v5.31292", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036595987"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/j.gastro.2016.02.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036979729"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neo.2014.08.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039223131"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb2048", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041002524", 
          "https://doi.org/10.1038/ncb2048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb2048", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041002524", 
          "https://doi.org/10.1038/ncb2048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbagrm.2012.09.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041862047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.94.7.3052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042255660"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btu170", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042720804"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-065x.2012.01146.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046391288"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.23.15023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046467972"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-12-3970", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046968399"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/eji.201041037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048006180"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/eji.201041037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048006180"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature17173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048251040", 
          "https://doi.org/10.1038/nature17173"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gku1063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048670772"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ccr.2008.10.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050078697"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/j.gastro.2011.04.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051659712"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1074-7613(02)00424-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053009400"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm.3394", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053059226", 
          "https://doi.org/10.1038/nm.3394"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/infdis/162.1.215", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059702085"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12974-017-0799-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074209901", 
          "https://doi.org/10.1186/s12974-017-0799-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12974-017-0799-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074209901", 
          "https://doi.org/10.1186/s12974-017-0799-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.168.7.3570", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1075018080"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076045225", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.175.12.8200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077154046"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083293473", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm.4409", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092113398", 
          "https://doi.org/10.1038/nm.4409"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm.4409", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092113398", 
          "https://doi.org/10.1038/nm.4409"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2017.11.052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1099659881"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2017.11.052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1099659881"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41467-018-03966-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103613894", 
          "https://doi.org/10.1038/s41467-018-03966-7"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "BACKGROUND: Cell-cell interactions maintain tissue homeostasis and contribute to dynamic alteration of the tumor microenvironment (TME). Communication between cancer and host cells not only promotes advanced disease aggression but also determines therapeutic response in cancer patients. Despite accumulating evidence supporting the role of tumor-infiltrating immunocytes in modulating tumor immunity, the interplay between heterogeneous tumor subpopulations and immunocytes is elusive.\nMETHODS: We expanded colorectal cancer stem cells (CRCSCs) as cancer spheroids from the murine colorectal cancer (CRC) cell line CT26 to interrogate tumor-host interactions using a syngeneic tumor model. RNA-sequencing analysis of host cells and tumor exosomes was performed to identify molecular determinants that mediate the crosstalk between CRCSCs and immunocytes. The Cancer Genome Atlas (TCGA) database was used to validate the clinical significance in CRC patients.\nRESULTS: The expanded CT26 cancer spheroids showed increased stemness gene expression, enhanced spheroid and clonogenicity potential, and an elevated tumor-initiating ability, characteristic of CRCSCs. By examining immune cell composition in syngeneic tumor-bearing mice, a systemic increase in CD11b+/Ly6GHigh/Ly6CLow neutrophils was observed in mice bearing CRCSC-derived tumors. An increased secretion of CRCSC exosomes was observed in vitro, and through in vivo tracking, CRCSC exosomes were found to be transported to the bone marrow. Moreover, CRCSC exosomes prolonged the survival of bone marrow-derived neutrophils and engendered a protumoral phenotype in neutrophils. Mechanistically, tumor exosomal tri-phosphate RNAs induced the expression of interleukin-1\u03b2 (IL-1\u03b2) through a pattern recognition-NF-\u03baB signaling axis to sustain neutrophil survival. CRCSC-secreted CXCL1 and CXCL2 then attracted CRCSC-primed neutrophils to promote tumorigenesis of CRC cells via IL-1\u03b2. Moreover, neutrophil depletion using a Ly6G-specific antibody (clone 1A8) attenuated the tumorigenicity of CRCSCs. In human specimens, CRC patients exhibiting an active CRCSC signal (Snail+IL8+) showed elevated tumor infiltration of MPO+ neutrophils, and high (in the top 10%) MPO expression predicted poor survival of CRC patients.\nCONCLUSIONS: This study elucidates a multistep CRCSC-neutrophil interaction during advanced cancer progression. Strategies targeting aberrant neutrophil activation may be developed for combating CSC-related malignancy.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s13045-019-0699-4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1039771", 
        "issn": [
          "1756-8722"
        ], 
        "name": "Journal of Hematology & Oncology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "12"
      }
    ], 
    "name": "Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer", 
    "pagination": "10", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "4329bfea955b3f8729d802a3a10ed7af8adf4fc0cca569136e1eadfcb3264dff"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30683126"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101468937"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s13045-019-0699-4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1111656941"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s13045-019-0699-4", 
      "https://app.dimensions.ai/details/publication/pub.1111656941"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T08:58", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000326_0000000326/records_68451_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs13045-019-0699-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13045-019-0699-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13045-019-0699-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13045-019-0699-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13045-019-0699-4'


 

This table displays all metadata directly associated to this object as RDF triples.

296 TRIPLES      21 PREDICATES      89 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s13045-019-0699-4 schema:about anzsrc-for:11
2 anzsrc-for:1112
3 schema:author Nb8761224d22549a083664f752711c5fb
4 schema:citation sg:pub.10.1038/nature07602
5 sg:pub.10.1038/nature14971
6 sg:pub.10.1038/nature15756
7 sg:pub.10.1038/nature17173
8 sg:pub.10.1038/ncb2048
9 sg:pub.10.1038/ncb2910
10 sg:pub.10.1038/ncb3346
11 sg:pub.10.1038/ni1087
12 sg:pub.10.1038/nm.2084
13 sg:pub.10.1038/nm.3394
14 sg:pub.10.1038/nm.4409
15 sg:pub.10.1038/nrc.2016.54
16 sg:pub.10.1038/nrg1840
17 sg:pub.10.1038/nri855
18 sg:pub.10.1038/s41467-018-03966-7
19 sg:pub.10.1186/s12974-017-0799-4
20 https://app.dimensions.ai/details/publication/pub.1076045225
21 https://app.dimensions.ai/details/publication/pub.1083293473
22 https://doi.org/10.1002/eji.201041037
23 https://doi.org/10.1016/j.apsb.2016.02.001
24 https://doi.org/10.1016/j.bbagrm.2012.09.010
25 https://doi.org/10.1016/j.ccell.2014.12.001
26 https://doi.org/10.1016/j.ccr.2006.03.030
27 https://doi.org/10.1016/j.ccr.2008.10.011
28 https://doi.org/10.1016/j.cell.2011.02.013
29 https://doi.org/10.1016/j.celrep.2017.11.052
30 https://doi.org/10.1016/j.it.2016.01.004
31 https://doi.org/10.1016/j.it.2016.03.005
32 https://doi.org/10.1016/j.neo.2014.08.013
33 https://doi.org/10.1016/j.stem.2011.02.020
34 https://doi.org/10.1016/s1074-7613(02)00424-7
35 https://doi.org/10.1046/j.1365-2249.2002.01836.x
36 https://doi.org/10.1053/j.gastro.2011.04.008
37 https://doi.org/10.1053/j.gastro.2016.02.040
38 https://doi.org/10.1073/pnas.0437939100
39 https://doi.org/10.1073/pnas.94.7.3052
40 https://doi.org/10.1073/pnas.95.13.7556
41 https://doi.org/10.1074/jbc.272.23.15023
42 https://doi.org/10.1083/jcb.97.2.329
43 https://doi.org/10.1084/jem.20040624
44 https://doi.org/10.1093/bioinformatics/bts587
45 https://doi.org/10.1093/bioinformatics/btu170
46 https://doi.org/10.1093/infdis/162.1.215
47 https://doi.org/10.1093/nar/gku1063
48 https://doi.org/10.1111/j.1600-065x.2012.01146.x
49 https://doi.org/10.1126/science.1132505
50 https://doi.org/10.1155/2010/201026
51 https://doi.org/10.1158/0008-5472.can-06-3126
52 https://doi.org/10.1158/0008-5472.can-12-3970
53 https://doi.org/10.1158/1078-0432.ccr-15-2463
54 https://doi.org/10.1172/jci61067
55 https://doi.org/10.1182/blood-2010-09-308387
56 https://doi.org/10.1182/blood.v98.10.3143
57 https://doi.org/10.1189/jlb.0306164
58 https://doi.org/10.1371/journal.pone.0030806
59 https://doi.org/10.2147/ijn.s13402
60 https://doi.org/10.3402/jev.v5.31292
61 https://doi.org/10.4049/jimmunol.168.7.3570
62 https://doi.org/10.4049/jimmunol.175.12.8200
63 https://doi.org/10.4049/jimmunol.176.1.284
64 schema:datePublished 2019-12
65 schema:datePublishedReg 2019-12-01
66 schema:description BACKGROUND: Cell-cell interactions maintain tissue homeostasis and contribute to dynamic alteration of the tumor microenvironment (TME). Communication between cancer and host cells not only promotes advanced disease aggression but also determines therapeutic response in cancer patients. Despite accumulating evidence supporting the role of tumor-infiltrating immunocytes in modulating tumor immunity, the interplay between heterogeneous tumor subpopulations and immunocytes is elusive. METHODS: We expanded colorectal cancer stem cells (CRCSCs) as cancer spheroids from the murine colorectal cancer (CRC) cell line CT26 to interrogate tumor-host interactions using a syngeneic tumor model. RNA-sequencing analysis of host cells and tumor exosomes was performed to identify molecular determinants that mediate the crosstalk between CRCSCs and immunocytes. The Cancer Genome Atlas (TCGA) database was used to validate the clinical significance in CRC patients. RESULTS: The expanded CT26 cancer spheroids showed increased stemness gene expression, enhanced spheroid and clonogenicity potential, and an elevated tumor-initiating ability, characteristic of CRCSCs. By examining immune cell composition in syngeneic tumor-bearing mice, a systemic increase in CD11b+/Ly6GHigh/Ly6CLow neutrophils was observed in mice bearing CRCSC-derived tumors. An increased secretion of CRCSC exosomes was observed in vitro, and through in vivo tracking, CRCSC exosomes were found to be transported to the bone marrow. Moreover, CRCSC exosomes prolonged the survival of bone marrow-derived neutrophils and engendered a protumoral phenotype in neutrophils. Mechanistically, tumor exosomal tri-phosphate RNAs induced the expression of interleukin-1β (IL-1β) through a pattern recognition-NF-κB signaling axis to sustain neutrophil survival. CRCSC-secreted CXCL1 and CXCL2 then attracted CRCSC-primed neutrophils to promote tumorigenesis of CRC cells via IL-1β. Moreover, neutrophil depletion using a Ly6G-specific antibody (clone 1A8) attenuated the tumorigenicity of CRCSCs. In human specimens, CRC patients exhibiting an active CRCSC signal (Snail+IL8+) showed elevated tumor infiltration of MPO+ neutrophils, and high (in the top 10%) MPO expression predicted poor survival of CRC patients. CONCLUSIONS: This study elucidates a multistep CRCSC-neutrophil interaction during advanced cancer progression. Strategies targeting aberrant neutrophil activation may be developed for combating CSC-related malignancy.
67 schema:genre research_article
68 schema:inLanguage en
69 schema:isAccessibleForFree true
70 schema:isPartOf N7a94ff46a08a43fead10a763291bc2b6
71 Ncbf7b8b8e9284e428b241438ab945705
72 sg:journal.1039771
73 schema:name Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer
74 schema:pagination 10
75 schema:productId N1992e91647574dcfbc62a4833bff960f
76 N60b6fb7e19104f4ea5d7e1591d4b8059
77 N634b84a1000149f6b47c3e0452ba7cdf
78 Nd24466a9f42c4323bdbff1922a258149
79 Ne5081d16823541bab591922ac0353bf4
80 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111656941
81 https://doi.org/10.1186/s13045-019-0699-4
82 schema:sdDatePublished 2019-04-11T08:58
83 schema:sdLicense https://scigraph.springernature.com/explorer/license/
84 schema:sdPublisher N7500b6c0a73d484b8d424d7d043af8eb
85 schema:url https://link.springer.com/10.1186%2Fs13045-019-0699-4
86 sgo:license sg:explorer/license/
87 sgo:sdDataset articles
88 rdf:type schema:ScholarlyArticle
89 N1992e91647574dcfbc62a4833bff960f schema:name doi
90 schema:value 10.1186/s13045-019-0699-4
91 rdf:type schema:PropertyValue
92 N2ac2d6578b2e419da540cb056aa97d56 rdf:first Na0e480502fa6493ab405620e0ce43e52
93 rdf:rest Nc33fc7fb3f734434aa395d0630e33960
94 N60b6fb7e19104f4ea5d7e1591d4b8059 schema:name pubmed_id
95 schema:value 30683126
96 rdf:type schema:PropertyValue
97 N634b84a1000149f6b47c3e0452ba7cdf schema:name readcube_id
98 schema:value 4329bfea955b3f8729d802a3a10ed7af8adf4fc0cca569136e1eadfcb3264dff
99 rdf:type schema:PropertyValue
100 N7500b6c0a73d484b8d424d7d043af8eb schema:name Springer Nature - SN SciGraph project
101 rdf:type schema:Organization
102 N7a94ff46a08a43fead10a763291bc2b6 schema:issueNumber 1
103 rdf:type schema:PublicationIssue
104 Na0e480502fa6493ab405620e0ce43e52 schema:affiliation https://www.grid.ac/institutes/grid.260770.4
105 schema:familyName Huang
106 schema:givenName Shih-Ching
107 rdf:type schema:Person
108 Nb8761224d22549a083664f752711c5fb rdf:first sg:person.01231257400.70
109 rdf:rest Nba8f463fb3c34ae78a31073f4527e347
110 Nba8f463fb3c34ae78a31073f4527e347 rdf:first sg:person.0760206621.37
111 rdf:rest Nfa0d53852b8f42819c0dc1d3da777ae4
112 Nc33fc7fb3f734434aa395d0630e33960 rdf:first sg:person.01270363147.69
113 rdf:rest rdf:nil
114 Ncbf7b8b8e9284e428b241438ab945705 schema:volumeNumber 12
115 rdf:type schema:PublicationVolume
116 Nd24466a9f42c4323bdbff1922a258149 schema:name nlm_unique_id
117 schema:value 101468937
118 rdf:type schema:PropertyValue
119 Ne5081d16823541bab591922ac0353bf4 schema:name dimensions_id
120 schema:value pub.1111656941
121 rdf:type schema:PropertyValue
122 Nfa0d53852b8f42819c0dc1d3da777ae4 rdf:first sg:person.0622547475.21
123 rdf:rest N2ac2d6578b2e419da540cb056aa97d56
124 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
125 schema:name Medical and Health Sciences
126 rdf:type schema:DefinedTerm
127 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
128 schema:name Oncology and Carcinogenesis
129 rdf:type schema:DefinedTerm
130 sg:journal.1039771 schema:issn 1756-8722
131 schema:name Journal of Hematology & Oncology
132 rdf:type schema:Periodical
133 sg:person.01231257400.70 schema:affiliation https://www.grid.ac/institutes/grid.260770.4
134 schema:familyName Hwang
135 schema:givenName Wei-Lun
136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231257400.70
137 rdf:type schema:Person
138 sg:person.01270363147.69 schema:affiliation https://www.grid.ac/institutes/grid.260770.4
139 schema:familyName Yang
140 schema:givenName Muh-Hwa
141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270363147.69
142 rdf:type schema:Person
143 sg:person.0622547475.21 schema:affiliation https://www.grid.ac/institutes/grid.254145.3
144 schema:familyName Cheng
145 schema:givenName Wei-Chung
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622547475.21
147 rdf:type schema:Person
148 sg:person.0760206621.37 schema:affiliation https://www.grid.ac/institutes/grid.260770.4
149 schema:familyName Lan
150 schema:givenName Hsin-Yi
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760206621.37
152 rdf:type schema:Person
153 sg:pub.10.1038/nature07602 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020963310
154 https://doi.org/10.1038/nature07602
155 rdf:type schema:CreativeWork
156 sg:pub.10.1038/nature14971 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021631986
157 https://doi.org/10.1038/nature14971
158 rdf:type schema:CreativeWork
159 sg:pub.10.1038/nature15756 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018970538
160 https://doi.org/10.1038/nature15756
161 rdf:type schema:CreativeWork
162 sg:pub.10.1038/nature17173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048251040
163 https://doi.org/10.1038/nature17173
164 rdf:type schema:CreativeWork
165 sg:pub.10.1038/ncb2048 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041002524
166 https://doi.org/10.1038/ncb2048
167 rdf:type schema:CreativeWork
168 sg:pub.10.1038/ncb2910 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002357372
169 https://doi.org/10.1038/ncb2910
170 rdf:type schema:CreativeWork
171 sg:pub.10.1038/ncb3346 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014129034
172 https://doi.org/10.1038/ncb3346
173 rdf:type schema:CreativeWork
174 sg:pub.10.1038/ni1087 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032695282
175 https://doi.org/10.1038/ni1087
176 rdf:type schema:CreativeWork
177 sg:pub.10.1038/nm.2084 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026729604
178 https://doi.org/10.1038/nm.2084
179 rdf:type schema:CreativeWork
180 sg:pub.10.1038/nm.3394 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053059226
181 https://doi.org/10.1038/nm.3394
182 rdf:type schema:CreativeWork
183 sg:pub.10.1038/nm.4409 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092113398
184 https://doi.org/10.1038/nm.4409
185 rdf:type schema:CreativeWork
186 sg:pub.10.1038/nrc.2016.54 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030891341
187 https://doi.org/10.1038/nrc.2016.54
188 rdf:type schema:CreativeWork
189 sg:pub.10.1038/nrg1840 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026833222
190 https://doi.org/10.1038/nrg1840
191 rdf:type schema:CreativeWork
192 sg:pub.10.1038/nri855 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002497020
193 https://doi.org/10.1038/nri855
194 rdf:type schema:CreativeWork
195 sg:pub.10.1038/s41467-018-03966-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103613894
196 https://doi.org/10.1038/s41467-018-03966-7
197 rdf:type schema:CreativeWork
198 sg:pub.10.1186/s12974-017-0799-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074209901
199 https://doi.org/10.1186/s12974-017-0799-4
200 rdf:type schema:CreativeWork
201 https://app.dimensions.ai/details/publication/pub.1076045225 schema:CreativeWork
202 https://app.dimensions.ai/details/publication/pub.1083293473 schema:CreativeWork
203 https://doi.org/10.1002/eji.201041037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048006180
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/j.apsb.2016.02.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024172511
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/j.bbagrm.2012.09.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041862047
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/j.ccell.2014.12.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025732101
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/j.ccr.2006.03.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013645548
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/j.ccr.2008.10.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050078697
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/j.cell.2011.02.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023540354
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/j.celrep.2017.11.052 schema:sameAs https://app.dimensions.ai/details/publication/pub.1099659881
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/j.it.2016.01.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033522829
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/j.it.2016.03.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014821554
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/j.neo.2014.08.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039223131
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/j.stem.2011.02.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010215023
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/s1074-7613(02)00424-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053009400
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1046/j.1365-2249.2002.01836.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002645474
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1053/j.gastro.2011.04.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051659712
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1053/j.gastro.2016.02.040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036979729
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1073/pnas.0437939100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018569245
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1073/pnas.94.7.3052 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042255660
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1073/pnas.95.13.7556 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022907036
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1074/jbc.272.23.15023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046467972
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1083/jcb.97.2.329 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036056299
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1084/jem.20040624 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030179768
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1093/bioinformatics/bts587 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012255299
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1093/bioinformatics/btu170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042720804
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1093/infdis/162.1.215 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059702085
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1093/nar/gku1063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048670772
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1111/j.1600-065x.2012.01146.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1046391288
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1126/science.1132505 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020542785
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1155/2010/201026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036440340
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1158/0008-5472.can-06-3126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010617336
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1158/0008-5472.can-12-3970 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046968399
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1158/1078-0432.ccr-15-2463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025747537
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1172/jci61067 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001453830
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1182/blood-2010-09-308387 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023718905
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1182/blood.v98.10.3143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028513687
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1189/jlb.0306164 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024353994
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1371/journal.pone.0030806 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032689931
276 rdf:type schema:CreativeWork
277 https://doi.org/10.2147/ijn.s13402 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019482319
278 rdf:type schema:CreativeWork
279 https://doi.org/10.3402/jev.v5.31292 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036595987
280 rdf:type schema:CreativeWork
281 https://doi.org/10.4049/jimmunol.168.7.3570 schema:sameAs https://app.dimensions.ai/details/publication/pub.1075018080
282 rdf:type schema:CreativeWork
283 https://doi.org/10.4049/jimmunol.175.12.8200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077154046
284 rdf:type schema:CreativeWork
285 https://doi.org/10.4049/jimmunol.176.1.284 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007173345
286 rdf:type schema:CreativeWork
287 https://www.grid.ac/institutes/grid.254145.3 schema:alternateName China Medical University
288 schema:name Graduate Institute of Biomedical Science, Research Center for Tumor Medical Science, and Drug Development Center, China Medical University, No.6, Hsueh-Shih Road, 40403, Taichung, Taiwan
289 rdf:type schema:Organization
290 https://www.grid.ac/institutes/grid.260770.4 schema:alternateName National Yang Ming University
291 schema:name Cancer Progression Research Center, National Yang-Ming University, No. 155, Sec. 2, Li-Nong Street, 11221, Taipei, Taiwan
292 Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, No. 155, Sec. 2, Li-Nong St., 11221, Taipei, Taiwan
293 Division of Medical Oncology, Department of Oncology, Taipei Veterans General Hospital, No.201, Sec. 2, Shipai Rd., 11217, Taipei, Taiwan
294 Institute of Clinical Medicine, National Yang-Ming University, No. 155, Sec. 2, Li-Nong Street, 11221, Taipei, Taiwan
295 The Ph.D. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, No.250, Wuxing St., 11031, Taipei, Taiwan
296 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...