The karyotype of Aegilops geniculata and its use to identify both addition and substitution lines of wheat View Full Text


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Article Info

DATE

2019-12

AUTHORS

Yingjin Yi, Ke Zheng, Shunzong Ning, Laibin Zhao, Kai Xu, Ming Hao, Lianquan Zhang, Zhongwei Yuan, Dengcai Liu

ABSTRACT

The annual allotetraploid species Aegilops geniculata harbors a number of traits relevant for wheat improvement. An effective cytogenetic method has yet to be developed to distinguish between each of its 14 chromosomes. A fluorescence in situ hybridization (FISH) approach was adopted to describe the karyotype of Ae. geniculata. Each of its 14 chromosomes was unequivocally recognized using a cocktail of three probes, namely pTa-713, (AAC)5 and pTa71. FISH karyotyping was then used to detect and characterize selections from an Ae. geniculata × bread wheat wide cross of a chromosome 1Mg disomic addition line and three 4Mg(4B) substitution lines. The identity of the addition line was confirmed by the presence of Glu-M1, detected both using an SDS-PAGE separation of endosperm proteins and by applying a PCR assay directed at the Glu-M1 locus. The status of the substitution lines was validated by genotyping using a wheat single nucleotide polymorphism chip. FISH karyotyping based on pTa-713, (AAC)5 and pTa71 will be useful for determining the contribution of Ae. geniculata to derivatives of an Ae. geniculata × wheat wide cross. SNP chip-based genotyping is effective for confirming the status of whole chromosome wheat/alien substitution lines. More... »

PAGES

15

References to SciGraph publications

  • 2018-12. Fluorescence in situ hybridization karyotyping reveals the presence of two distinct genomes in the taxon Aegilops tauschii in BMC GENOMICS
  • 2013-05. Cytogenetic analysis of Aegilops chromosomes, potentially usable in triticale (X Triticosecale Witt.) breeding in JOURNAL OF APPLIED GENETICS
  • 2015-12. Genetic analyses of Glu-1Ssh in wheat/Aegilops sharonensis hybrid progenies and development of alien HMW-GSs gene-specific markers in MOLECULAR BREEDING
  • 1989-09. C-banding pattern and powdery mildew resistance of Triticum ovatum and four T. aestivum-T. ovatum chromosome addition lines in THEORETICAL AND APPLIED GENETICS
  • 2014-12. SNP Discovery for mapping alien introgressions in wheat in BMC GENOMICS
  • 2011-07. Discovery and molecular mapping of a new gene conferring resistance to stem rust, Sr53, derived from Aegilops geniculata and characterization of spontaneous translocation stocks with reduced alien chromatin in CHROMOSOME RESEARCH
  • 2000-10. Identification of different chromatin classes in wheat using in situ hybridization with simple sequence repeat oligonucleotides in THEORETICAL AND APPLIED GENETICS
  • 1998-10. Breeding technology of alloplasmic wheat in SCIENCE CHINA LIFE SCIENCES
  • 2002-01. Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.) 7. Gene Pm29 in line Pova in EUPHYTICA
  • 2004-04. Genome differentiation in Aegilops. 4. Evolution of the U-genome cluster in PLANT SYSTEMATICS AND EVOLUTION
  • 2002-02. Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits in SCIENCE BULLETIN
  • 2014-08. Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis in JOURNAL OF APPLIED GENETICS
  • 2018-02. Interspecific and intergeneric hybridization as a source of variation for wheat grain quality improvement in THEORETICAL AND APPLIED GENETICS
  • 2007-05. Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat in THEORETICAL AND APPLIED GENETICS
  • Journal

    TITLE

    Molecular Cytogenetics

    ISSUE

    1

    VOLUME

    12

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13039-019-0428-2

    DOI

    http://dx.doi.org/10.1186/s13039-019-0428-2

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1113205209


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    39 schema:description The annual allotetraploid species Aegilops geniculata harbors a number of traits relevant for wheat improvement. An effective cytogenetic method has yet to be developed to distinguish between each of its 14 chromosomes. A fluorescence in situ hybridization (FISH) approach was adopted to describe the karyotype of Ae. geniculata. Each of its 14 chromosomes was unequivocally recognized using a cocktail of three probes, namely pTa-713, (AAC)5 and pTa71. FISH karyotyping was then used to detect and characterize selections from an Ae. geniculata × bread wheat wide cross of a chromosome 1Mg disomic addition line and three 4Mg(4B) substitution lines. The identity of the addition line was confirmed by the presence of Glu-M1, detected both using an SDS-PAGE separation of endosperm proteins and by applying a PCR assay directed at the Glu-M1 locus. The status of the substitution lines was validated by genotyping using a wheat single nucleotide polymorphism chip. FISH karyotyping based on pTa-713, (AAC)5 and pTa71 will be useful for determining the contribution of Ae. geniculata to derivatives of an Ae. geniculata × wheat wide cross. SNP chip-based genotyping is effective for confirming the status of whole chromosome wheat/alien substitution lines.
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