Emergence of clonal chromosomal alterations during the mesenchymal stromal cell cultivation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-12

AUTHORS

Tamara Borgonovo, Maria Marlene Solarewicz, Isadora May Vaz, Debora Daga, Carmen Lúcia Kuniyoshi Rebelatto, Alexandra Cristina Senegaglia, Enilze Ribeiro, Iglenir João Cavalli, Paulo Slud Brofman

ABSTRACT

BACKGROUND: Use of human mesenchymal stromal cells (MSCs) is a promising strategy for cell therapy in injured tissues recovery. However, MSCs acquire genetic changes when cultivated in vitro that make them more susceptible to undergo neoplastic transformation. Therefore, genomic integrity of stem cells should be monitored carefully for the use in basic research and clinical trials, including karyotype analysis to confirm the absence of genetic instability. Here, we report a case of a male 67-year-old patient selected to join the study: "Autologous transplantation of mesenchymal cells for treatment of severe and refractory ischemic cardiomyopathy". He underwent nephrectomy for malignant tumor on the right kidney. Cytogenetic analysis on a bone marrow sample showed a normal karyotype: 46,XY[20]. However, the MSC at second passage showed a hyperdiploid clone, with clonal trisomies of chromosomes 4, 5, 10 and X. In order to investigate more, another sample from the same patient was used for a second cultivation and, at third passage, these cells showed a clonal translocation t(9;18)(p24;q11). The recurrent aberrations in MSC may indicate the beginning of a spontaneous transformation in culture, so, these cells were not used for cell therapy. Several analyses were performed at the Center for Cell Technology (152 samples), however this was the only case to show clonal cytogenetic abnormalities. Interestingly, two distinct clonal alterations were seen in two parallel cell cultivations from the same patient, suggesting a propensity for genetic instability. This highlights the need to evaluate these cells on a case-by-case basis, especially in patients with a history of cancer. Although there is controversy about the use of cells with cytogenetic abnormality for therapy, because their tumorigenic doubtful potential, we decided against the use of these cells for regenerative medicine. CASE PRESENTATION: Here, we report a case of a male 67-year-old patient selected to join the study: "Autologous transplantation of mesenchymal cells for treatment of severe and refractory ischemic cardiomyopathy". He underwent nephrectomy for malignant tumor on the right kidney. Cytogenetic analysis on a bone marrow sample showed a normal karyotype: 46,XY[20]. However, the MSC at second passage showed a hyperdiploid clone, with clonal trisomies of chromosomes 4, 5, 10 and X. In order to investigate more, another sample from the same patient was used for a second cultivation and, at third passage, these cells showed a clonal translocation t(9;18)(p24;q11). The recurrent aberrations in MSC may indicate the beginning of a spontaneous transformation in culture, so, these cells were not used for cell therapy. Several analyses were performed at the Center for Cell Technology(152 samples), however this was the only case to show clonal cytogenetic abnormalities. Interestingly, two distinct clonal alterations were seen in two parallel cell cultivations from the same patient, suggesting a propensity for genetic instability. This highlights the need to evaluate these cells on a case-by-case basis, especially in patients with a history of cancer. CONCLUSIONS: Although there is controversy about the use of cells with cytogenetic abnormality for therapy, because their tumorigenic doubtful potential, we decided against the use of these cells forregenerative medicine. More... »

PAGES

94

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s13039-015-0197-5

DOI

http://dx.doi.org/10.1186/s13039-015-0197-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027131946

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26628918


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1", 
          "id": "https://www.grid.ac/institutes/grid.412522.2", 
          "name": [
            "Centro de Tecnologia Celular (CTC), Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba, Paran\u00e1, Brazil", 
            "Cytogenetic Laboratory of Centro de Tecnololgia Celular (CTC), PUCPR, Curitiba, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Borgonovo", 
        "givenName": "Tamara", 
        "id": "sg:person.01350115572.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350115572.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1", 
          "id": "https://www.grid.ac/institutes/grid.412522.2", 
          "name": [
            "Centro de Tecnologia Celular (CTC), Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba, Paran\u00e1, Brazil", 
            "Cytogenetic Laboratory of Centro de Tecnololgia Celular (CTC), PUCPR, Curitiba, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Solarewicz", 
        "givenName": "Maria Marlene", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1", 
          "id": "https://www.grid.ac/institutes/grid.412522.2", 
          "name": [
            "Centro de Tecnologia Celular (CTC), Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba, Paran\u00e1, Brazil", 
            "Cytogenetic Laboratory of Centro de Tecnololgia Celular (CTC), PUCPR, Curitiba, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vaz", 
        "givenName": "Isadora May", 
        "id": "sg:person.0624511472.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0624511472.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1", 
          "id": "https://www.grid.ac/institutes/grid.412522.2", 
          "name": [
            "Centro de Tecnologia Celular (CTC), Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba, Paran\u00e1, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Daga", 
        "givenName": "Debora", 
        "id": "sg:person.01250421655.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250421655.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1", 
          "id": "https://www.grid.ac/institutes/grid.412522.2", 
          "name": [
            "Centro de Tecnologia Celular (CTC), Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba, Paran\u00e1, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rebelatto", 
        "givenName": "Carmen L\u00facia Kuniyoshi", 
        "id": "sg:person.01046012553.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046012553.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1", 
          "id": "https://www.grid.ac/institutes/grid.412522.2", 
          "name": [
            "Centro de Tecnologia Celular (CTC), Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba, Paran\u00e1, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Senegaglia", 
        "givenName": "Alexandra Cristina", 
        "id": "sg:person.0734663345.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0734663345.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Paran\u00e1", 
          "id": "https://www.grid.ac/institutes/grid.20736.30", 
          "name": [
            "Departamento de Gen\u00e9tica, Universidade Federal do Paran\u00e1 (UFPR), Curitiba, Paran\u00e1, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ribeiro", 
        "givenName": "Enilze", 
        "id": "sg:person.0601123544.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601123544.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Paran\u00e1", 
          "id": "https://www.grid.ac/institutes/grid.20736.30", 
          "name": [
            "Departamento de Gen\u00e9tica, Universidade Federal do Paran\u00e1 (UFPR), Curitiba, Paran\u00e1, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cavalli", 
        "givenName": "Iglenir Jo\u00e3o", 
        "id": "sg:person.01023352227.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023352227.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1", 
          "id": "https://www.grid.ac/institutes/grid.412522.2", 
          "name": [
            "Centro de Tecnologia Celular (CTC), Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba, Paran\u00e1, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brofman", 
        "givenName": "Paulo Slud", 
        "id": "sg:person.01307631013.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307631013.52"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.3181/0712-rm-356", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000878720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3181/0712-rm-356", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000878720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/14653240500319234", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004050442"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jcyt.2013.07.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011619437"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2011.12.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013350024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/14653240600855905", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025538709"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/scd.2014.0299", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029109709"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mrfmmm.2014.12.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032368348"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2011.12.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033494196"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bjhh.2014.03.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036910484"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jcyt.2013.03.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039469686"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.imlet.2015.06.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039882142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-3148.2012.01134.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050022782"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.5966/sctm.2014-0110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1073577507"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2015-12", 
    "datePublishedReg": "2015-12-01", 
    "description": "BACKGROUND: Use of human mesenchymal stromal cells (MSCs) is a promising strategy for cell therapy in injured tissues recovery. However, MSCs acquire genetic changes when cultivated in vitro that make them more susceptible to undergo neoplastic transformation. Therefore, genomic integrity of stem cells should be monitored carefully for the use in basic research and clinical trials, including karyotype analysis to confirm the absence of genetic instability. Here, we report a case of a male 67-year-old patient selected to join the study: \"Autologous transplantation of mesenchymal cells for treatment of severe and refractory ischemic cardiomyopathy\". He underwent nephrectomy for malignant tumor on the right kidney. Cytogenetic analysis on a bone marrow sample showed a normal karyotype: 46,XY[20]. However, the MSC at second passage showed a hyperdiploid clone, with clonal trisomies of chromosomes 4, 5, 10 and X. In order to investigate more, another sample from the same patient was used for a second cultivation and, at third passage, these cells showed a clonal translocation t(9;18)(p24;q11). The recurrent aberrations in MSC may indicate the beginning of a spontaneous transformation in culture, so, these cells were not used for cell therapy. Several analyses were performed at the Center for Cell Technology (152 samples), however this was the only case to show clonal cytogenetic abnormalities. Interestingly, two distinct clonal alterations were seen in two parallel cell cultivations from the same patient, suggesting a propensity for genetic instability. This highlights the need to evaluate these cells on a case-by-case basis, especially in patients with a history of cancer. Although there is controversy about the use of cells with cytogenetic abnormality for therapy, because their tumorigenic doubtful potential, we decided against the use of these cells for regenerative medicine.\nCASE PRESENTATION: Here, we report a case of a male 67-year-old patient selected to join the study: \"Autologous transplantation of mesenchymal cells for treatment of severe and refractory ischemic cardiomyopathy\". He underwent nephrectomy for malignant tumor on the right kidney. Cytogenetic analysis on a bone marrow sample showed a normal karyotype: 46,XY[20]. However, the MSC at second passage showed a hyperdiploid clone, with clonal trisomies of chromosomes 4, 5, 10 and X. In order to investigate more, another sample from the same patient was used for a second cultivation and, at third passage, these cells showed a clonal translocation t(9;18)(p24;q11). The recurrent aberrations in MSC may indicate the beginning of a spontaneous transformation in culture, so, these cells were not used for cell therapy. Several analyses were performed at the Center for Cell Technology(152 samples), however this was the only case to show clonal cytogenetic abnormalities. Interestingly, two distinct clonal alterations were seen in two parallel cell cultivations from the same patient, suggesting a propensity for genetic instability. This highlights the need to evaluate these cells on a case-by-case basis, especially in patients with a history of cancer.\nCONCLUSIONS: Although there is controversy about the use of cells with cytogenetic abnormality for therapy, because their tumorigenic doubtful potential, we decided against the use of these cells forregenerative medicine.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s13039-015-0197-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1039110", 
        "issn": [
          "1755-8166"
        ], 
        "name": "Molecular Cytogenetics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "8"
      }
    ], 
    "name": "Emergence of clonal chromosomal alterations during the mesenchymal stromal cell cultivation", 
    "pagination": "94", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "813f1261b79128ea75cc1c101d816b4f356655597b74eaa12e1889746e96aa6a"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "26628918"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101317942"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s13039-015-0197-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1027131946"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s13039-015-0197-5", 
      "https://app.dimensions.ai/details/publication/pub.1027131946"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89801_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1186%2Fs13039-015-0197-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s13039-015-0197-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s13039-015-0197-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s13039-015-0197-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s13039-015-0197-5'


 

This table displays all metadata directly associated to this object as RDF triples.

166 TRIPLES      21 PREDICATES      42 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s13039-015-0197-5 schema:about anzsrc-for:11
2 anzsrc-for:1103
3 schema:author N2820a5d8b2944b38baae1937c026ce8a
4 schema:citation https://doi.org/10.1016/j.bjhh.2014.03.006
5 https://doi.org/10.1016/j.imlet.2015.06.006
6 https://doi.org/10.1016/j.jcyt.2013.03.005
7 https://doi.org/10.1016/j.jcyt.2013.07.004
8 https://doi.org/10.1016/j.mrfmmm.2014.12.006
9 https://doi.org/10.1016/j.stem.2011.12.005
10 https://doi.org/10.1016/j.stem.2011.12.007
11 https://doi.org/10.1080/14653240500319234
12 https://doi.org/10.1080/14653240600855905
13 https://doi.org/10.1089/scd.2014.0299
14 https://doi.org/10.1111/j.1365-3148.2012.01134.x
15 https://doi.org/10.3181/0712-rm-356
16 https://doi.org/10.5966/sctm.2014-0110
17 schema:datePublished 2015-12
18 schema:datePublishedReg 2015-12-01
19 schema:description BACKGROUND: Use of human mesenchymal stromal cells (MSCs) is a promising strategy for cell therapy in injured tissues recovery. However, MSCs acquire genetic changes when cultivated in vitro that make them more susceptible to undergo neoplastic transformation. Therefore, genomic integrity of stem cells should be monitored carefully for the use in basic research and clinical trials, including karyotype analysis to confirm the absence of genetic instability. Here, we report a case of a male 67-year-old patient selected to join the study: "Autologous transplantation of mesenchymal cells for treatment of severe and refractory ischemic cardiomyopathy". He underwent nephrectomy for malignant tumor on the right kidney. Cytogenetic analysis on a bone marrow sample showed a normal karyotype: 46,XY[20]. However, the MSC at second passage showed a hyperdiploid clone, with clonal trisomies of chromosomes 4, 5, 10 and X. In order to investigate more, another sample from the same patient was used for a second cultivation and, at third passage, these cells showed a clonal translocation t(9;18)(p24;q11). The recurrent aberrations in MSC may indicate the beginning of a spontaneous transformation in culture, so, these cells were not used for cell therapy. Several analyses were performed at the Center for Cell Technology (152 samples), however this was the only case to show clonal cytogenetic abnormalities. Interestingly, two distinct clonal alterations were seen in two parallel cell cultivations from the same patient, suggesting a propensity for genetic instability. This highlights the need to evaluate these cells on a case-by-case basis, especially in patients with a history of cancer. Although there is controversy about the use of cells with cytogenetic abnormality for therapy, because their tumorigenic doubtful potential, we decided against the use of these cells for regenerative medicine. CASE PRESENTATION: Here, we report a case of a male 67-year-old patient selected to join the study: "Autologous transplantation of mesenchymal cells for treatment of severe and refractory ischemic cardiomyopathy". He underwent nephrectomy for malignant tumor on the right kidney. Cytogenetic analysis on a bone marrow sample showed a normal karyotype: 46,XY[20]. However, the MSC at second passage showed a hyperdiploid clone, with clonal trisomies of chromosomes 4, 5, 10 and X. In order to investigate more, another sample from the same patient was used for a second cultivation and, at third passage, these cells showed a clonal translocation t(9;18)(p24;q11). The recurrent aberrations in MSC may indicate the beginning of a spontaneous transformation in culture, so, these cells were not used for cell therapy. Several analyses were performed at the Center for Cell Technology(152 samples), however this was the only case to show clonal cytogenetic abnormalities. Interestingly, two distinct clonal alterations were seen in two parallel cell cultivations from the same patient, suggesting a propensity for genetic instability. This highlights the need to evaluate these cells on a case-by-case basis, especially in patients with a history of cancer. CONCLUSIONS: Although there is controversy about the use of cells with cytogenetic abnormality for therapy, because their tumorigenic doubtful potential, we decided against the use of these cells forregenerative medicine.
20 schema:genre research_article
21 schema:inLanguage en
22 schema:isAccessibleForFree true
23 schema:isPartOf N32f30cdf1bb24d96bef4b9ebf6641073
24 Ndc7454f09fa748a3bcd678646f0e6841
25 sg:journal.1039110
26 schema:name Emergence of clonal chromosomal alterations during the mesenchymal stromal cell cultivation
27 schema:pagination 94
28 schema:productId N0bc459d54a6240e496cfe9611ed962d0
29 N0c4ebf20fdfc42ef95571aa59684f70f
30 N324b37e7946642248a46ef19165375d2
31 N4cc855b18b6d47caa0f03b7cac91fa4d
32 Na36da82413a64edb948a416da72d8609
33 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027131946
34 https://doi.org/10.1186/s13039-015-0197-5
35 schema:sdDatePublished 2019-04-11T09:55
36 schema:sdLicense https://scigraph.springernature.com/explorer/license/
37 schema:sdPublisher N673ef75c6ee64c408bbc3ec4a74ae434
38 schema:url http://link.springer.com/10.1186%2Fs13039-015-0197-5
39 sgo:license sg:explorer/license/
40 sgo:sdDataset articles
41 rdf:type schema:ScholarlyArticle
42 N0b6df65ae78241559e75f4393091301e rdf:first sg:person.01250421655.85
43 rdf:rest Nd805b209053c48bd989c8713964d39af
44 N0bc459d54a6240e496cfe9611ed962d0 schema:name nlm_unique_id
45 schema:value 101317942
46 rdf:type schema:PropertyValue
47 N0c4ebf20fdfc42ef95571aa59684f70f schema:name pubmed_id
48 schema:value 26628918
49 rdf:type schema:PropertyValue
50 N2274e591f8ba4f24a761b8d792e63983 rdf:first sg:person.01307631013.52
51 rdf:rest rdf:nil
52 N2820a5d8b2944b38baae1937c026ce8a rdf:first sg:person.01350115572.39
53 rdf:rest N864e7b74b62c4a379a54071101f41554
54 N28825495c72b44a3979a812cbd87778f rdf:first sg:person.0624511472.44
55 rdf:rest N0b6df65ae78241559e75f4393091301e
56 N324b37e7946642248a46ef19165375d2 schema:name doi
57 schema:value 10.1186/s13039-015-0197-5
58 rdf:type schema:PropertyValue
59 N32f30cdf1bb24d96bef4b9ebf6641073 schema:volumeNumber 8
60 rdf:type schema:PublicationVolume
61 N3666385dbe3841fabd41764dc94b657b rdf:first sg:person.01023352227.34
62 rdf:rest N2274e591f8ba4f24a761b8d792e63983
63 N4cc855b18b6d47caa0f03b7cac91fa4d schema:name dimensions_id
64 schema:value pub.1027131946
65 rdf:type schema:PropertyValue
66 N673ef75c6ee64c408bbc3ec4a74ae434 schema:name Springer Nature - SN SciGraph project
67 rdf:type schema:Organization
68 N864e7b74b62c4a379a54071101f41554 rdf:first Nb91ec5bc50ff483ba95c29c62cbca078
69 rdf:rest N28825495c72b44a3979a812cbd87778f
70 N91dc717deef14139b6d38a2d717954e2 rdf:first sg:person.0601123544.97
71 rdf:rest N3666385dbe3841fabd41764dc94b657b
72 Na36da82413a64edb948a416da72d8609 schema:name readcube_id
73 schema:value 813f1261b79128ea75cc1c101d816b4f356655597b74eaa12e1889746e96aa6a
74 rdf:type schema:PropertyValue
75 Nb91ec5bc50ff483ba95c29c62cbca078 schema:affiliation https://www.grid.ac/institutes/grid.412522.2
76 schema:familyName Solarewicz
77 schema:givenName Maria Marlene
78 rdf:type schema:Person
79 Nd805b209053c48bd989c8713964d39af rdf:first sg:person.01046012553.55
80 rdf:rest Ndfd0140dc9a141f3ad87b746c1e8a2b0
81 Ndc7454f09fa748a3bcd678646f0e6841 schema:issueNumber 1
82 rdf:type schema:PublicationIssue
83 Ndfd0140dc9a141f3ad87b746c1e8a2b0 rdf:first sg:person.0734663345.55
84 rdf:rest N91dc717deef14139b6d38a2d717954e2
85 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
86 schema:name Medical and Health Sciences
87 rdf:type schema:DefinedTerm
88 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
89 schema:name Clinical Sciences
90 rdf:type schema:DefinedTerm
91 sg:journal.1039110 schema:issn 1755-8166
92 schema:name Molecular Cytogenetics
93 rdf:type schema:Periodical
94 sg:person.01023352227.34 schema:affiliation https://www.grid.ac/institutes/grid.20736.30
95 schema:familyName Cavalli
96 schema:givenName Iglenir João
97 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023352227.34
98 rdf:type schema:Person
99 sg:person.01046012553.55 schema:affiliation https://www.grid.ac/institutes/grid.412522.2
100 schema:familyName Rebelatto
101 schema:givenName Carmen Lúcia Kuniyoshi
102 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046012553.55
103 rdf:type schema:Person
104 sg:person.01250421655.85 schema:affiliation https://www.grid.ac/institutes/grid.412522.2
105 schema:familyName Daga
106 schema:givenName Debora
107 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250421655.85
108 rdf:type schema:Person
109 sg:person.01307631013.52 schema:affiliation https://www.grid.ac/institutes/grid.412522.2
110 schema:familyName Brofman
111 schema:givenName Paulo Slud
112 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307631013.52
113 rdf:type schema:Person
114 sg:person.01350115572.39 schema:affiliation https://www.grid.ac/institutes/grid.412522.2
115 schema:familyName Borgonovo
116 schema:givenName Tamara
117 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350115572.39
118 rdf:type schema:Person
119 sg:person.0601123544.97 schema:affiliation https://www.grid.ac/institutes/grid.20736.30
120 schema:familyName Ribeiro
121 schema:givenName Enilze
122 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601123544.97
123 rdf:type schema:Person
124 sg:person.0624511472.44 schema:affiliation https://www.grid.ac/institutes/grid.412522.2
125 schema:familyName Vaz
126 schema:givenName Isadora May
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0624511472.44
128 rdf:type schema:Person
129 sg:person.0734663345.55 schema:affiliation https://www.grid.ac/institutes/grid.412522.2
130 schema:familyName Senegaglia
131 schema:givenName Alexandra Cristina
132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0734663345.55
133 rdf:type schema:Person
134 https://doi.org/10.1016/j.bjhh.2014.03.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036910484
135 rdf:type schema:CreativeWork
136 https://doi.org/10.1016/j.imlet.2015.06.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039882142
137 rdf:type schema:CreativeWork
138 https://doi.org/10.1016/j.jcyt.2013.03.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039469686
139 rdf:type schema:CreativeWork
140 https://doi.org/10.1016/j.jcyt.2013.07.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011619437
141 rdf:type schema:CreativeWork
142 https://doi.org/10.1016/j.mrfmmm.2014.12.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032368348
143 rdf:type schema:CreativeWork
144 https://doi.org/10.1016/j.stem.2011.12.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033494196
145 rdf:type schema:CreativeWork
146 https://doi.org/10.1016/j.stem.2011.12.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013350024
147 rdf:type schema:CreativeWork
148 https://doi.org/10.1080/14653240500319234 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004050442
149 rdf:type schema:CreativeWork
150 https://doi.org/10.1080/14653240600855905 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025538709
151 rdf:type schema:CreativeWork
152 https://doi.org/10.1089/scd.2014.0299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029109709
153 rdf:type schema:CreativeWork
154 https://doi.org/10.1111/j.1365-3148.2012.01134.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1050022782
155 rdf:type schema:CreativeWork
156 https://doi.org/10.3181/0712-rm-356 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000878720
157 rdf:type schema:CreativeWork
158 https://doi.org/10.5966/sctm.2014-0110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1073577507
159 rdf:type schema:CreativeWork
160 https://www.grid.ac/institutes/grid.20736.30 schema:alternateName Federal University of Paraná
161 schema:name Departamento de Genética, Universidade Federal do Paraná (UFPR), Curitiba, Paraná, Brazil
162 rdf:type schema:Organization
163 https://www.grid.ac/institutes/grid.412522.2 schema:alternateName Pontifícia Universidade Católica do Paraná
164 schema:name Centro de Tecnologia Celular (CTC), Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Paraná, Brazil
165 Cytogenetic Laboratory of Centro de Tecnololgia Celular (CTC), PUCPR, Curitiba, Brazil
166 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...