Immunohistochemical analysis revealed the expression of bone morphogenetic proteins-4, 6, 7, and 9 in human induced membrane samples treated with ... View Full Text


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Article Info

DATE

2022-01-15

AUTHORS

Takahiro Niikura, Takahiro Oda, Naoe Jimbo, Masato Komatsu, Keisuke Oe, Tomoaki Fukui, Tomoyuki Matsumoto, Shinya Hayashi, Takehiko Matsushita, Tomoo Itoh, Ryosuke Kuroda

ABSTRACT

BackgroundInduced membrane (IM) is the key component of Masquelet reconstruction surgery for the treatment of bone defects. IM is formed around the cement spacer and is known to secrete growth factors and osteoinductive factors. However, there is limited evidence available concerning the presence of osteoinductive factors in IM. This study aimed to investigate the existence of bone morphogenetic proteins (BMPs) in IM harvested from patients during the treatment of bone defects using the Masquelet technique.MethodsThis study involved six patients whose bone defects had been treated using the Masquelet technique. The affected sites were the femur (n = 3) and the tibia (n = 3). During the second-stage surgery, 1 cm2 pieces of IM were harvested. Histological sections of IM were immunostained with anti-BMP-4, 6, 7, and 9 antibodies. Human bone tissue served as the positive control.ResultsThe presence of BMP-4, 6, 7, and 9 was observed in all IM samples. Further, immunolocalization of BMP-4, 6, 7, and 9 was observed in blood vessels and fibroblasts in all IM samples. Immunolocalization of BMP-4, 6, 7, and 9 was also observed in bone tissue within the IM in one sample, in which osteogenesis inside the IM was observed.ConclusionsThis study showed that osteoinductive factors BMP-4, 6, 7, and 9 were present in the IM harvested from patients, providing evidence indicating that the Masquelet technique effectively contributes to healing large bone defects. Therefore, it may be possible for surgeons to omit the addition of BMPs to bone grafts, given the endogenous secretion of BMPs from the IM. More... »

PAGES

29

References to SciGraph publications

  • 2016-08-13. Comparative study suggests that human bone morphogenetic proteins have no influence on the outcome of operative treatment of aseptic clavicle non-unions in INTERNATIONAL ORTHOPAEDICS
  • 2006-02-28. Osteogenic protein-1 (BMP-7) accelerates healing of scaphoid non-union with proximal pole sclerosis in INTERNATIONAL ORTHOPAEDICS
  • 2014-08-22. Reconstruction of intercalary bone defects following bone tumor resection with segmental bone transport using an Ilizarov circular external fixator in JOURNAL OF ORTHOPAEDIC SCIENCE
  • 2013-12-19. The clinical use of bone morphogenetic proteins revisited: a novel biocompatible carrier device OSTEOGROW for bone healing in INTERNATIONAL ORTHOPAEDICS
  • 2019-04-11. The ‘diamond concept’ for long bone non-union management in JOURNAL OF ORTHOPAEDICS AND TRAUMATOLOGY
  • 2017-06-29. Clinical need for bone morphogenetic proteins in INTERNATIONAL ORTHOPAEDICS
  • 2021-04-13. Histological analysis of induced membranes in patients whose bone defects were treated with the Masquelet technique to identify factors affecting the vascularity of induced membranes in JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
  • 2015-09-17. Induction of granulation tissue for the secretion of growth factors and the promotion of bone defect repair in JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
  • 2019-04-16. Ilizarov bone transport and treatment of critical-sized tibial bone defects: a narrative review in JOURNAL OF ORTHOPAEDICS AND TRAUMATOLOGY
  • 2012-03-02. Vascularized fibular graft for bone defects after wide resection of musculoskeletal tumors in JOURNAL OF ORTHOPAEDIC SCIENCE
  • 2018-09-15. Bone morphogenetic proteins in fracture repair in INTERNATIONAL ORTHOPAEDICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13018-022-02922-y

    DOI

    http://dx.doi.org/10.1186/s13018-022-02922-y

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1144707875

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/35033126


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    30 schema:description BackgroundInduced membrane (IM) is the key component of Masquelet reconstruction surgery for the treatment of bone defects. IM is formed around the cement spacer and is known to secrete growth factors and osteoinductive factors. However, there is limited evidence available concerning the presence of osteoinductive factors in IM. This study aimed to investigate the existence of bone morphogenetic proteins (BMPs) in IM harvested from patients during the treatment of bone defects using the Masquelet technique.MethodsThis study involved six patients whose bone defects had been treated using the Masquelet technique. The affected sites were the femur (n = 3) and the tibia (n = 3). During the second-stage surgery, 1 cm2 pieces of IM were harvested. Histological sections of IM were immunostained with anti-BMP-4, 6, 7, and 9 antibodies. Human bone tissue served as the positive control.ResultsThe presence of BMP-4, 6, 7, and 9 was observed in all IM samples. Further, immunolocalization of BMP-4, 6, 7, and 9 was observed in blood vessels and fibroblasts in all IM samples. Immunolocalization of BMP-4, 6, 7, and 9 was also observed in bone tissue within the IM in one sample, in which osteogenesis inside the IM was observed.ConclusionsThis study showed that osteoinductive factors BMP-4, 6, 7, and 9 were present in the IM harvested from patients, providing evidence indicating that the Masquelet technique effectively contributes to healing large bone defects. Therefore, it may be possible for surgeons to omit the addition of BMPs to bone grafts, given the endogenous secretion of BMPs from the IM.
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    37 ConclusionsThis study
    38 IM
    39 IM samples
    40 Masquelet technique
    41 MethodsThis
    42 addition
    43 addition of BMP
    44 affected site
    45 analysis
    46 antibodies
    47 blood vessels
    48 bone defects
    49 bone graft
    50 bone morphogenetic protein
    51 bone morphogenetic protein 4
    52 bone tissue
    53 cement spacer
    54 cm2 pieces
    55 components
    56 control
    57 defects
    58 endogenous secretion
    59 evidence
    60 existence
    61 expression
    62 factors
    63 femur
    64 fibroblasts
    65 graft
    66 growth factor
    67 histological sections
    68 human bone tissue
    69 immunohistochemical analysis
    70 immunolocalization
    71 key component
    72 large bone defects
    73 limited evidence
    74 membrane
    75 membrane samples
    76 morphogenetic proteins
    77 osteogenesis
    78 osteoinductive factors
    79 patients
    80 pieces
    81 positive control
    82 presence
    83 protein
    84 protein 4
    85 reconstruction surgery
    86 samples
    87 second-stage surgery
    88 secretion
    89 sections
    90 sites
    91 spacer
    92 study
    93 surgeons
    94 surgery
    95 technique
    96 tibia
    97 tissue
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    100 schema:name Immunohistochemical analysis revealed the expression of bone morphogenetic proteins-4, 6, 7, and 9 in human induced membrane samples treated with the Masquelet technique
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