Adaptive minirhizotron for pepper roots observation and its installation based on root system architecture traits View Full Text


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Article Info

DATE

2019-12

AUTHORS

Wei Lu, Xiaochan Wang, Fengjie Wang

ABSTRACT

Background: Root is the principal part of plants to absorb water and nutrient, anchor the plant and affect yield and quality. Non-destructive detection of root traits is difficult to perform due to the hidden nature of the root. Therefore, improved methods to measure roots are necessary to support plant breeding, and optimization of cultivation and management. In this study, we present an adaptive minirhizotron along with installation patterns to focus on micro and local changes in multipoint of pepper roots. Results: The method is to improve minirhizotron by reducing its size to a microrhizotron (1.1 × 1.1 × 1.2 cm) and improving installation validity and rationality according to spatial distribution characteristics of Capsicum annuum root system. This adaptive minirhizotron could acquire root image in multipoint, and through image processing, root traits such as root length (including very fine roots or root hairs) and root width could be calculated. In order to install the microrhizotron reasonably and effectively, root system architecture (Capsicum annuum) was reconstructed using a three-dimensional caliper, and were quantified in circumferential distribution, vertical direction and root extension. The results showed that most lateral roots were constrained to 45° in horizontal direction to root initial position; Vertical angles were large, almost perpendicular to the root center line at initial position, and it became smaller when roots began to deepen. Root length density decreased with the increase of distance to plant center. According to Capsicum annuum root system traits, totally 8 installation methods were tested and verified to determine high probability of root interception. Horizontal angle 45° showed much higher interception probability than that of 90°. Vertical angle 45° has slightly higher root interception probability than that of 30°. Installation pattern horizontal angle 45° + radius 30 mm + vertical angle 45° showed the best performance in root interception with probability of 96.7%, followed by pattern horizontal angle 45° + radius 30 mm + vertical angle 30°. Comparison experiment showed that when root hair and very fine root were excluded, relative error was 12.1% between microrhizotron and soil sampling in root length, and 15.4% in root diameter. Microrhizotron was able to observe fine roots about 0.1 mm in diameter. Conclusion: A new adaptive minirhizotron has been established for nondestructive observation on local and micro changes of roots in multipoint, and its application and installation patterns has been suggested according to root architecture traits. The microrhizotron can be used to study a wide range of research questions focused on quantitative trait locus analysis, root width changes, and root hair growth. More... »

PAGES

29

References to SciGraph publications

  • 2017-12. Non-invasive imaging of plant roots in different soils using magnetic resonance imaging (MRI) in PLANT METHODS
  • 1994-04. Soil core and minirhizotron comparison for the determination of root length density in PLANT AND SOIL
  • 2001-02. Evaluating minirhizotron estimates of fine root longevity and production in the forest floor of a temperate broadleaf forest in PLANT AND SOIL
  • 2012-01. Quantitative estimates of root densities at minirhizotrons differ from those in the bulk soil in PLANT AND SOIL
  • 2011. Crop Responses to Soil Physical Conditions in ENCYCLOPEDIA OF AGROPHYSICS
  • 2015-12. Direct comparison of MRI and X-ray CT technologies for 3D imaging of root systems in soil: potential and challenges for root trait quantification in PLANT METHODS
  • 2012. Minirhizotrons in Modern Root Studies in MEASURING ROOTS
  • 1996-09. Root sampling methods - applications and limitations of the minirhizotron technique in PLANT AND SOIL
  • 2018-12. Phenotyping field-state wheat root system architecture for root foraging traits in response to environment×management interactions in SCIENTIFIC REPORTS
  • 1937-06. A Device for the Observation of Root Growth in the Soil in NATURE
  • 2014-01. Improved scaling of minirhizotron data using an empirically-derived depth of field and correcting for the underestimation of root diameters in PLANT AND SOIL
  • 2001-09. The relationships between static and dynamic variables in the description of root growth. Consequences for field interpretation of rooting variability in PLANT AND SOIL
  • 2017-12. An evaluation of inexpensive methods for root image acquisition when using rhizotrons in PLANT METHODS
  • 2012-03. Advancing the use of minirhizotrons in wetlands in PLANT AND SOIL
  • 2014-12. Rhizoslides: paper-based growth system for non-destructive, high throughput phenotyping of root development by means of image analysis in PLANT METHODS
  • Journal

    TITLE

    Plant Methods

    ISSUE

    1

    VOLUME

    15

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s13007-019-0414-z

    DOI

    http://dx.doi.org/10.1186/s13007-019-0414-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112965177

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30949230


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