The Epstein-Barr virus EBNA1 protein modulates the alternative splicing of cellular genes View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Simon Boudreault, Victoria E. S. Armero, Michelle S. Scott, Jean-Pierre Perreault, Martin Bisaillon

ABSTRACT

BACKGROUND: Alternative splicing (AS) is an important mRNA maturation step that allows increased variability and diversity of proteins in eukaryotes. AS is dysregulated in numerous diseases, and its implication in the carcinogenic process is well known. However, progress in understanding how oncogenic viruses modulate splicing, and how this modulation is involved in viral oncogenicity has been limited. Epstein-Barr virus (EBV) is involved in various cancers, and its EBNA1 oncoprotein is the only viral protein expressed in all EBV malignancies. METHODS: In the present study, the ability of EBNA1 to modulate the AS of cellular genes was assessed using a high-throughput RT-PCR approach to examine AS in 1238 cancer-associated genes. RNA immunoprecipitation coupled to RNA sequencing (RIP-Seq) assays were also performed to identify cellular mRNAs bound by EBNA1. RESULTS: Upon EBNA1 expression, we detected modifications to the AS profiles of 89 genes involved in cancer. Moreover, we show that EBNA1 modulates the expression levels of various splicing factors such as hnRNPA1, FOX-2, and SF1. Finally, RNA immunoprecipitation coupled to RIP-Seq assays demonstrate that EBNA1 immunoprecipitates specific cellular mRNAs, but not the ones that are spliced differently in EBNA1-expressing cells. CONCLUSION: The EBNA1 protein can modulate the AS profiles of numerous cellular genes. Interestingly, this modulation protein does not require the RNA binding activity of EBNA1. Overall, these findings underline the novel role of EBNA1 as a cellular splicing modulator. More... »

PAGES

29

Journal

TITLE

Virology Journal

ISSUE

1

VOLUME

16

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12985-019-1137-5

DOI

http://dx.doi.org/10.1186/s12985-019-1137-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112527677

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30832682


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