Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Elif Nurtop, Paola Mariela Saba Villarroel, Boris Pastorino, Laetitia Ninove, Jan Felix Drexler, Yelin Roca, Bouba Gake, Audrey Dubot-Peres, Gilda Grard, Christophe Peyrefitte, Stéphane Priet, Xavier de Lamballerie, Pierre Gallian

ABSTRACT

Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies. More... »

PAGES

192

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12985-018-1105-5

DOI

http://dx.doi.org/10.1186/s12985-018-1105-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1110911688

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30587193


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