Trigger factor assisted self-assembly of canine parvovirus VP2 protein into virus-like particles in Escherichia coli with high immunogenicity View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Liangliang Nan, Yunchao Liu, Pengchao Ji, Hua Feng, Chen Chen, Juan Wang, Dongmin Liu, Yinglei Cui, Yanwei Wang, Yafei Li, Enmin Zhou, Gaiping Zhang

ABSTRACT

Canine parvovirus (CPV) has been considered to be an important pathogen, which can cause acute infectious disease in canids. Although current vaccines are effective in preventing CPV infection, safety problems still remain unsolved. In this study, a subunit vaccine against CPV based on virus-like particles (VLPs) with good safety and immunogenicity is reported. Soluble CPV VP2 protein was produced by co-expression of chaperone trigger factor (Tf16) in Escherichia coli (E.coli), and assembled into CPV VLPs which could be affected by NaCl and pH. At 250 mM NaCl pH 8.0, the VLPs co-expressed with Tf16 had similar size (25 nm) and shape with the authentic virus capsid under the transmission electron microscopy (TEM), which is also in accordance with the dynamic light scattering (DLS) data. Immunization with these particles could induce high-titer hemagglutination inhibition (1:12288) and neutralizing antibodies (1:6144) in guinea pigs. Splenic cells of them could secrete IFN-γ and IL-4 after stimulation by CPV. Thus, the VLPs produced by the new approach with high yield and immunogenicity could be a potential candidate for CPV vaccine. More... »

PAGES

103

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12985-018-1013-8

DOI

http://dx.doi.org/10.1186/s12985-018-1013-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1104988688

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29921294


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Neutralizing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Capsid Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytokines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dogs", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Guinea Pigs", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hemagglutination Inhibition Tests", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neutralization Tests", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Parvoviridae Infections", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Parvovirus, Canine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Vaccines, Subunit", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Vaccines, Virus-Like Particle", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virion", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Henan Academy of Agricultural Sciences", 
          "id": "https://www.grid.ac/institutes/grid.495707.8", 
          "name": [
            "College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, China", 
            "Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nan", 
        "givenName": "Liangliang", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Henan Academy of Agricultural Sciences", 
          "id": "https://www.grid.ac/institutes/grid.495707.8", 
          "name": [
            "Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Yunchao", 
        "id": "sg:person.01132702727.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132702727.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Henan Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.108266.b", 
          "name": [
            "College of Animal Science and Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ji", 
        "givenName": "Pengchao", 
        "id": "sg:person.01023006375.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023006375.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Henan Academy of Agricultural Sciences", 
          "id": "https://www.grid.ac/institutes/grid.495707.8", 
          "name": [
            "Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Feng", 
        "givenName": "Hua", 
        "id": "sg:person.015146521315.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015146521315.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "North West Agriculture and Forestry University", 
          "id": "https://www.grid.ac/institutes/grid.144022.1", 
          "name": [
            "College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "Chen", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Henan Zhongze Biological Engineering Co., Ltd., 450019, Zhengzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Juan", 
        "id": "sg:person.016447073754.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016447073754.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Henan Zhongze Biological Engineering Co., Ltd., 450019, Zhengzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Dongmin", 
        "id": "sg:person.01043254311.65", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043254311.65"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Henan Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.108266.b", 
          "name": [
            "Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China", 
            "College of Animal Science and Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cui", 
        "givenName": "Yinglei", 
        "id": "sg:person.07660007136.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07660007136.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Henan Academy of Agricultural Sciences", 
          "id": "https://www.grid.ac/institutes/grid.495707.8", 
          "name": [
            "Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Yanwei", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Henan Academy of Agricultural Sciences", 
          "id": "https://www.grid.ac/institutes/grid.495707.8", 
          "name": [
            "College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, China", 
            "Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Yafei", 
        "id": "sg:person.016356275561.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016356275561.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "North West Agriculture and Forestry University", 
          "id": "https://www.grid.ac/institutes/grid.144022.1", 
          "name": [
            "College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhou", 
        "givenName": "Enmin", 
        "id": "sg:person.01320617331.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320617331.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Henan Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.108266.b", 
          "name": [
            "College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, China", 
            "Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China", 
            "College of Animal Science and Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Gaiping", 
        "id": "sg:person.01156231352.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156231352.38"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1126/science.1068408", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001702524"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vaccine.2007.01.106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006422993"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biochi.2011.12.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010969495"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2003.11.142", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011826588"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.antiviral.2017.01.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015388846"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(02)00259-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015652703"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/viro.2000.0734", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017362803"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jsb.2003.11.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019930687"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1748-5827.2010.00959a.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020010839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1748-5827.2010.00959a.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020010839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jps.20696", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021311079"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-013-5485-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022666055", 
          "https://doi.org/10.1007/s00253-013-5485-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0079575", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026674161"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vaccine.2013.05.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027825530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vaccine.2013.05.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027825530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cvsm.2010.07.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029628269"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1159/000150482", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031667962"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11274-007-9493-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033815890", 
          "https://doi.org/10.1007/s11274-007-9493-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jviromet.2007.07.021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033912200"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.meegid.2015.12.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035128383"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-319-23603-2_15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035744132", 
          "https://doi.org/10.1007/978-3-319-23603-2_15"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jbiosc.2013.08.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036203984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/cvi.00090-07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039358064"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11262-009-0378-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046364065", 
          "https://doi.org/10.1007/s11262-009-0378-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11262-009-0378-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046364065", 
          "https://doi.org/10.1007/s11262-009-0378-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.77.19.10250-10259.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051178647"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/0022-1317-69-5-1111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060356745"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/0022-1317-82-12-3021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060361833"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jn/121.9.1418", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077728325"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077895867", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080649960", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081644691", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083254917", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0175035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084309822"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/pbi.12783", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1086256923"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-12", 
    "datePublishedReg": "2018-12-01", 
    "description": "Canine parvovirus (CPV) has been considered to be an important pathogen, which can cause acute infectious disease in canids. Although current vaccines are effective in preventing CPV infection, safety problems still remain unsolved. In this study, a subunit vaccine against CPV based on virus-like particles (VLPs) with good safety and immunogenicity is reported. Soluble CPV VP2 protein was produced by co-expression of chaperone trigger factor (Tf16) in Escherichia coli (E.coli), and assembled into CPV VLPs which could be affected by NaCl and pH. At 250\u00a0mM NaCl pH\u00a08.0, the VLPs co-expressed with Tf16 had similar size (25\u00a0nm) and shape with the authentic virus capsid under the transmission electron microscopy (TEM), which is also in accordance with the dynamic light scattering (DLS) data. Immunization with these particles could induce high-titer hemagglutination inhibition (1:12288) and neutralizing antibodies (1:6144) in guinea pigs. Splenic cells of them could secrete IFN-\u03b3 and IL-4 after stimulation by CPV. Thus, the VLPs produced by the new approach with high yield and immunogenicity could be a potential candidate for CPV vaccine.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s12985-018-1013-8", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1034449", 
        "issn": [
          "1743-422X"
        ], 
        "name": "Virology Journal", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "name": "Trigger factor assisted self-assembly of canine parvovirus VP2 protein into virus-like particles in Escherichia coli with high immunogenicity", 
    "pagination": "103", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "52cf3f78c140588d255b7e0bee1f6a1a33559803c7e916f703355a60a663b04f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29921294"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101231645"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s12985-018-1013-8"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1104988688"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s12985-018-1013-8", 
      "https://app.dimensions.ai/details/publication/pub.1104988688"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T16:01", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000572.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs12985-018-1013-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12985-018-1013-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12985-018-1013-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12985-018-1013-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12985-018-1013-8'


 

This table displays all metadata directly associated to this object as RDF triples.

323 TRIPLES      21 PREDICATES      79 URIs      39 LITERALS      27 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s12985-018-1013-8 schema:about N0a0e0b8994c544a4ad50b7aeff121240
2 N0fdc923922ed4adeb0a8c3d49f3f1dad
3 N3e3a4212e35d4989bc37d07bfedfc959
4 N44a06667f5de410c8495cbbf12daff80
5 N45e0c67965954404b1f87cf09dd6d89d
6 N5a94df5637ae4b62934b793617619be6
7 N5af7200209be46baa9c5db2eb5dcfd96
8 N6e72a672deef404bbc5e0d4f244a37c4
9 N7c28c18f90e44d0ba0528a401927faa8
10 N89632a040e644fec9bca3267e0f36279
11 N958080e168a3477e8a62baab64861072
12 Nc301d035d4ac4cabae1521f185004f7f
13 Nc9f3ccbf50f64dd09df27e7ff3239ff0
14 Nd9e3ed5cb00f4e7ca80fb78549e52a1f
15 Ndeffa3addfbc47d28464da1cecdbcb8b
16 Ndfd936d6d333473298e5721c91f1a1e5
17 Nee29073902b04a3fb4ec8b9b39bf519f
18 Nfb525630d53c4be287715cd712448928
19 anzsrc-for:11
20 anzsrc-for:1108
21 schema:author Nbf0361c89f014d1d91ba9bd9d12533f5
22 schema:citation sg:pub.10.1007/978-3-319-23603-2_15
23 sg:pub.10.1007/s00253-013-5485-6
24 sg:pub.10.1007/s11262-009-0378-6
25 sg:pub.10.1007/s11274-007-9493-5
26 https://app.dimensions.ai/details/publication/pub.1077895867
27 https://app.dimensions.ai/details/publication/pub.1080649960
28 https://app.dimensions.ai/details/publication/pub.1081644691
29 https://app.dimensions.ai/details/publication/pub.1083254917
30 https://doi.org/10.1002/jps.20696
31 https://doi.org/10.1006/viro.2000.0734
32 https://doi.org/10.1016/j.antiviral.2017.01.003
33 https://doi.org/10.1016/j.bbrc.2003.11.142
34 https://doi.org/10.1016/j.biochi.2011.12.006
35 https://doi.org/10.1016/j.cvsm.2010.07.007
36 https://doi.org/10.1016/j.jbiosc.2013.08.015
37 https://doi.org/10.1016/j.jsb.2003.11.006
38 https://doi.org/10.1016/j.jviromet.2007.07.021
39 https://doi.org/10.1016/j.meegid.2015.12.009
40 https://doi.org/10.1016/j.vaccine.2007.01.106
41 https://doi.org/10.1016/j.vaccine.2013.05.023
42 https://doi.org/10.1016/s0022-2836(02)00259-0
43 https://doi.org/10.1093/jn/121.9.1418
44 https://doi.org/10.1099/0022-1317-69-5-1111
45 https://doi.org/10.1099/0022-1317-82-12-3021
46 https://doi.org/10.1111/j.1748-5827.2010.00959a.x
47 https://doi.org/10.1111/pbi.12783
48 https://doi.org/10.1126/science.1068408
49 https://doi.org/10.1128/cvi.00090-07
50 https://doi.org/10.1128/jvi.77.19.10250-10259.2003
51 https://doi.org/10.1159/000150482
52 https://doi.org/10.1371/journal.pone.0079575
53 https://doi.org/10.1371/journal.pone.0175035
54 schema:datePublished 2018-12
55 schema:datePublishedReg 2018-12-01
56 schema:description Canine parvovirus (CPV) has been considered to be an important pathogen, which can cause acute infectious disease in canids. Although current vaccines are effective in preventing CPV infection, safety problems still remain unsolved. In this study, a subunit vaccine against CPV based on virus-like particles (VLPs) with good safety and immunogenicity is reported. Soluble CPV VP2 protein was produced by co-expression of chaperone trigger factor (Tf16) in Escherichia coli (E.coli), and assembled into CPV VLPs which could be affected by NaCl and pH. At 250 mM NaCl pH 8.0, the VLPs co-expressed with Tf16 had similar size (25 nm) and shape with the authentic virus capsid under the transmission electron microscopy (TEM), which is also in accordance with the dynamic light scattering (DLS) data. Immunization with these particles could induce high-titer hemagglutination inhibition (1:12288) and neutralizing antibodies (1:6144) in guinea pigs. Splenic cells of them could secrete IFN-γ and IL-4 after stimulation by CPV. Thus, the VLPs produced by the new approach with high yield and immunogenicity could be a potential candidate for CPV vaccine.
57 schema:genre research_article
58 schema:inLanguage en
59 schema:isAccessibleForFree true
60 schema:isPartOf Nd476898a1ada46c1a849277e56ce70f0
61 Nf21a5996f52844a6a6482dcc0e91b3fc
62 sg:journal.1034449
63 schema:name Trigger factor assisted self-assembly of canine parvovirus VP2 protein into virus-like particles in Escherichia coli with high immunogenicity
64 schema:pagination 103
65 schema:productId N0c793352138c4a6a8e2e0dc31e1d21c1
66 N564ab0a1332247c3a851cce363078b7f
67 N83783edde1974ce0ba62ce2c96e1e287
68 Ndac5224c30f14a44bf1c7ddc209378b4
69 Nec01f03fbd184f0b8445c6524e4753b2
70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104988688
71 https://doi.org/10.1186/s12985-018-1013-8
72 schema:sdDatePublished 2019-04-10T16:01
73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
74 schema:sdPublisher N4fb470fd30564d68a4b80981a17e15a6
75 schema:url https://link.springer.com/10.1186%2Fs12985-018-1013-8
76 sgo:license sg:explorer/license/
77 sgo:sdDataset articles
78 rdf:type schema:ScholarlyArticle
79 N0a0e0b8994c544a4ad50b7aeff121240 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Cell Line
81 rdf:type schema:DefinedTerm
82 N0c793352138c4a6a8e2e0dc31e1d21c1 schema:name pubmed_id
83 schema:value 29921294
84 rdf:type schema:PropertyValue
85 N0fdc923922ed4adeb0a8c3d49f3f1dad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Cytokines
87 rdf:type schema:DefinedTerm
88 N1559cc1f33864aa38c3307842cfc3db3 schema:affiliation https://www.grid.ac/institutes/grid.495707.8
89 schema:familyName Wang
90 schema:givenName Yanwei
91 rdf:type schema:Person
92 N244a5afccf4640f486c40550f57b30b3 rdf:first sg:person.01043254311.65
93 rdf:rest N2de7eb3234454d5eb83f2abb23f93a65
94 N2de7eb3234454d5eb83f2abb23f93a65 rdf:first sg:person.07660007136.97
95 rdf:rest Nf173527d268e4b8a9b276815f1bed5ff
96 N3e3a4212e35d4989bc37d07bfedfc959 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Parvoviridae Infections
98 rdf:type schema:DefinedTerm
99 N44a06667f5de410c8495cbbf12daff80 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Antibodies, Viral
101 rdf:type schema:DefinedTerm
102 N45e0c67965954404b1f87cf09dd6d89d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Female
104 rdf:type schema:DefinedTerm
105 N4fb470fd30564d68a4b80981a17e15a6 schema:name Springer Nature - SN SciGraph project
106 rdf:type schema:Organization
107 N564ab0a1332247c3a851cce363078b7f schema:name readcube_id
108 schema:value 52cf3f78c140588d255b7e0bee1f6a1a33559803c7e916f703355a60a663b04f
109 rdf:type schema:PropertyValue
110 N5a94df5637ae4b62934b793617619be6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Virion
112 rdf:type schema:DefinedTerm
113 N5abd05f689674840abb411a2f0d2182b rdf:first sg:person.015146521315.34
114 rdf:rest N8fb934eff8804061b1f88eeb4fa7edc0
115 N5af7200209be46baa9c5db2eb5dcfd96 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Hemagglutination Inhibition Tests
117 rdf:type schema:DefinedTerm
118 N63e1152afb6f466b99514998a7b4d7e4 rdf:first sg:person.01023006375.96
119 rdf:rest N5abd05f689674840abb411a2f0d2182b
120 N6e72a672deef404bbc5e0d4f244a37c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Animals
122 rdf:type schema:DefinedTerm
123 N6f314e156f8c46d69f77dd0bc990fb60 rdf:first sg:person.016356275561.19
124 rdf:rest Neb509baebce8431b87d6b77b19643111
125 N751565ce7175474983ae477069e58899 schema:name Henan Zhongze Biological Engineering Co., Ltd., 450019, Zhengzhou, China
126 rdf:type schema:Organization
127 N751e06a300ee40e9af40020ccad68c50 rdf:first sg:person.01156231352.38
128 rdf:rest rdf:nil
129 N778d338f3f82439ab28d7b80458a99f4 schema:affiliation https://www.grid.ac/institutes/grid.495707.8
130 schema:familyName Nan
131 schema:givenName Liangliang
132 rdf:type schema:Person
133 N7c28c18f90e44d0ba0528a401927faa8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Cats
135 rdf:type schema:DefinedTerm
136 N83783edde1974ce0ba62ce2c96e1e287 schema:name dimensions_id
137 schema:value pub.1104988688
138 rdf:type schema:PropertyValue
139 N89632a040e644fec9bca3267e0f36279 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Vaccines, Virus-Like Particle
141 rdf:type schema:DefinedTerm
142 N8fb934eff8804061b1f88eeb4fa7edc0 rdf:first Nf893f2f98829487c881c250ed06fc084
143 rdf:rest Ndba9ed0512ec49eeae8d91bde5fb8bf8
144 N958080e168a3477e8a62baab64861072 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Neutralization Tests
146 rdf:type schema:DefinedTerm
147 Nbf0361c89f014d1d91ba9bd9d12533f5 rdf:first N778d338f3f82439ab28d7b80458a99f4
148 rdf:rest Nd227e9f3e74a40268882d00084f3e90c
149 Nc301d035d4ac4cabae1521f185004f7f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Parvovirus, Canine
151 rdf:type schema:DefinedTerm
152 Nc6b9aa34faf543609ce3d123c1d44d43 schema:name Henan Zhongze Biological Engineering Co., Ltd., 450019, Zhengzhou, China
153 rdf:type schema:Organization
154 Nc9f3ccbf50f64dd09df27e7ff3239ff0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Dogs
156 rdf:type schema:DefinedTerm
157 Nd227e9f3e74a40268882d00084f3e90c rdf:first sg:person.01132702727.06
158 rdf:rest N63e1152afb6f466b99514998a7b4d7e4
159 Nd476898a1ada46c1a849277e56ce70f0 schema:volumeNumber 15
160 rdf:type schema:PublicationVolume
161 Nd9e3ed5cb00f4e7ca80fb78549e52a1f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Capsid Proteins
163 rdf:type schema:DefinedTerm
164 Ndac5224c30f14a44bf1c7ddc209378b4 schema:name nlm_unique_id
165 schema:value 101231645
166 rdf:type schema:PropertyValue
167 Ndba9ed0512ec49eeae8d91bde5fb8bf8 rdf:first sg:person.016447073754.76
168 rdf:rest N244a5afccf4640f486c40550f57b30b3
169 Ndeffa3addfbc47d28464da1cecdbcb8b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Antibodies, Neutralizing
171 rdf:type schema:DefinedTerm
172 Ndfd936d6d333473298e5721c91f1a1e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Escherichia coli
174 rdf:type schema:DefinedTerm
175 Neb509baebce8431b87d6b77b19643111 rdf:first sg:person.01320617331.85
176 rdf:rest N751e06a300ee40e9af40020ccad68c50
177 Nec01f03fbd184f0b8445c6524e4753b2 schema:name doi
178 schema:value 10.1186/s12985-018-1013-8
179 rdf:type schema:PropertyValue
180 Nee29073902b04a3fb4ec8b9b39bf519f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Vaccines, Subunit
182 rdf:type schema:DefinedTerm
183 Nf173527d268e4b8a9b276815f1bed5ff rdf:first N1559cc1f33864aa38c3307842cfc3db3
184 rdf:rest N6f314e156f8c46d69f77dd0bc990fb60
185 Nf21a5996f52844a6a6482dcc0e91b3fc schema:issueNumber 1
186 rdf:type schema:PublicationIssue
187 Nf893f2f98829487c881c250ed06fc084 schema:affiliation https://www.grid.ac/institutes/grid.144022.1
188 schema:familyName Chen
189 schema:givenName Chen
190 rdf:type schema:Person
191 Nfb525630d53c4be287715cd712448928 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Guinea Pigs
193 rdf:type schema:DefinedTerm
194 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
195 schema:name Medical and Health Sciences
196 rdf:type schema:DefinedTerm
197 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
198 schema:name Medical Microbiology
199 rdf:type schema:DefinedTerm
200 sg:journal.1034449 schema:issn 1743-422X
201 schema:name Virology Journal
202 rdf:type schema:Periodical
203 sg:person.01023006375.96 schema:affiliation https://www.grid.ac/institutes/grid.108266.b
204 schema:familyName Ji
205 schema:givenName Pengchao
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023006375.96
207 rdf:type schema:Person
208 sg:person.01043254311.65 schema:affiliation N751565ce7175474983ae477069e58899
209 schema:familyName Liu
210 schema:givenName Dongmin
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043254311.65
212 rdf:type schema:Person
213 sg:person.01132702727.06 schema:affiliation https://www.grid.ac/institutes/grid.495707.8
214 schema:familyName Liu
215 schema:givenName Yunchao
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132702727.06
217 rdf:type schema:Person
218 sg:person.01156231352.38 schema:affiliation https://www.grid.ac/institutes/grid.108266.b
219 schema:familyName Zhang
220 schema:givenName Gaiping
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156231352.38
222 rdf:type schema:Person
223 sg:person.01320617331.85 schema:affiliation https://www.grid.ac/institutes/grid.144022.1
224 schema:familyName Zhou
225 schema:givenName Enmin
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320617331.85
227 rdf:type schema:Person
228 sg:person.015146521315.34 schema:affiliation https://www.grid.ac/institutes/grid.495707.8
229 schema:familyName Feng
230 schema:givenName Hua
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015146521315.34
232 rdf:type schema:Person
233 sg:person.016356275561.19 schema:affiliation https://www.grid.ac/institutes/grid.495707.8
234 schema:familyName Li
235 schema:givenName Yafei
236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016356275561.19
237 rdf:type schema:Person
238 sg:person.016447073754.76 schema:affiliation Nc6b9aa34faf543609ce3d123c1d44d43
239 schema:familyName Wang
240 schema:givenName Juan
241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016447073754.76
242 rdf:type schema:Person
243 sg:person.07660007136.97 schema:affiliation https://www.grid.ac/institutes/grid.108266.b
244 schema:familyName Cui
245 schema:givenName Yinglei
246 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07660007136.97
247 rdf:type schema:Person
248 sg:pub.10.1007/978-3-319-23603-2_15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035744132
249 https://doi.org/10.1007/978-3-319-23603-2_15
250 rdf:type schema:CreativeWork
251 sg:pub.10.1007/s00253-013-5485-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022666055
252 https://doi.org/10.1007/s00253-013-5485-6
253 rdf:type schema:CreativeWork
254 sg:pub.10.1007/s11262-009-0378-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046364065
255 https://doi.org/10.1007/s11262-009-0378-6
256 rdf:type schema:CreativeWork
257 sg:pub.10.1007/s11274-007-9493-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033815890
258 https://doi.org/10.1007/s11274-007-9493-5
259 rdf:type schema:CreativeWork
260 https://app.dimensions.ai/details/publication/pub.1077895867 schema:CreativeWork
261 https://app.dimensions.ai/details/publication/pub.1080649960 schema:CreativeWork
262 https://app.dimensions.ai/details/publication/pub.1081644691 schema:CreativeWork
263 https://app.dimensions.ai/details/publication/pub.1083254917 schema:CreativeWork
264 https://doi.org/10.1002/jps.20696 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021311079
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1006/viro.2000.0734 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017362803
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1016/j.antiviral.2017.01.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015388846
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1016/j.bbrc.2003.11.142 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011826588
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1016/j.biochi.2011.12.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010969495
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1016/j.cvsm.2010.07.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029628269
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1016/j.jbiosc.2013.08.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036203984
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1016/j.jsb.2003.11.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019930687
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1016/j.jviromet.2007.07.021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033912200
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1016/j.meegid.2015.12.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035128383
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1016/j.vaccine.2007.01.106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006422993
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1016/j.vaccine.2013.05.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027825530
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1016/s0022-2836(02)00259-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015652703
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1093/jn/121.9.1418 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077728325
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1099/0022-1317-69-5-1111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060356745
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1099/0022-1317-82-12-3021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060361833
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1111/j.1748-5827.2010.00959a.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020010839
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1111/pbi.12783 schema:sameAs https://app.dimensions.ai/details/publication/pub.1086256923
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1126/science.1068408 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001702524
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1128/cvi.00090-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039358064
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1128/jvi.77.19.10250-10259.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051178647
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1159/000150482 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031667962
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1371/journal.pone.0079575 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026674161
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1371/journal.pone.0175035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084309822
311 rdf:type schema:CreativeWork
312 https://www.grid.ac/institutes/grid.108266.b schema:alternateName Henan Agricultural University
313 schema:name College of Animal Science and Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, China
314 College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, China
315 Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China
316 rdf:type schema:Organization
317 https://www.grid.ac/institutes/grid.144022.1 schema:alternateName North West Agriculture and Forestry University
318 schema:name College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, China
319 rdf:type schema:Organization
320 https://www.grid.ac/institutes/grid.495707.8 schema:alternateName Henan Academy of Agricultural Sciences
321 schema:name College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, China
322 Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China
323 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...