Substitution of amino acid residue V1213 in the helicase domain of the genotype 3 hepatitis E virus reduces virus replication View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Dianjun Cao, Yan-Yan Ni, Xiang-Jin Meng

ABSTRACT

BACKGROUND: Genotype 3 hepatitis E virus (HEV) infection is generally associated with mild disease. However, recently eight genotype 3 HEV isolates were identified from patients with severe hepatitis. Importantly, three mutations (S605P, I978V and V1213A) in these genotype 3 isolates were found to be typical of genotype 4 HEV, which is sometime associated with more severe hepatitis. Therefore in this study we seek to determine if these unique mutations contribute to enhanced virus replication and thus potentially severe disease. METHODS: In the lack of an efficient cell culture system to study the effect of mutations on HEV replication, we developed a genotype 3 HEV replicon with Renilla luciferase (Rluc) as reporter and subsequently used it to construct numerous mutants, including swMu-1 (V1213A), swMu-2 (Q1246H), swMu-3 (V1213A and Q1246H), swMu-4 (S605P and I978V), and swMu-5 (V1213A, S605P and I978V). RNA transcripts from mutant replicons were transfected into Huh7 S10-3 liver cells to measure the effect of mutations on HEV replication efficiency. RESULTS: The results showed that the V1213A mutant had the highest reduction in HEV replication efficiency than other mutants. The V1213A and S605P + I978V mutations have a cumulative, if not synergistic, effect on HEV replication. The Q1246H mutant decreased HEV replication compared to the wild-type HEV Rluc replicon but replicated better than the V1213A mutant. The amino acid residue V1213 favors the replication of both genotypes 3 and 4 HEV strains, but not genotype 1 HEV. CONCLUSION: The results suggested that the V1213A mutation reduced HEV replication, but is likely not associated with the reported severe hepatitis caused by genotype 3 HEV isolates containing this mutation. More... »

PAGES

32

References to SciGraph publications

  • 2012-08. Molecular biology and replication of hepatitis E virus in EMERGING MICROBES & INFECTIONS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12985-018-0943-5

    DOI

    http://dx.doi.org/10.1186/s12985-018-0943-5

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1100913030

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29422085


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Substitution", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, Reporter", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genotype", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hepatitis E", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hepatitis E virus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mutation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Viral Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Virus Replication", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Virginia\u2013Maryland College of Veterinary Medicine", 
              "id": "https://www.grid.ac/institutes/grid.470073.7", 
              "name": [
                "Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, 1981 Kraft Drive, 24061-0913, Blacksburg, VA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Cao", 
            "givenName": "Dianjun", 
            "id": "sg:person.0640527462.71", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640527462.71"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Virginia\u2013Maryland College of Veterinary Medicine", 
              "id": "https://www.grid.ac/institutes/grid.470073.7", 
              "name": [
                "Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, 1981 Kraft Drive, 24061-0913, Blacksburg, VA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ni", 
            "givenName": "Yan-Yan", 
            "id": "sg:person.0733152350.42", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733152350.42"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Virginia\u2013Maryland College of Veterinary Medicine", 
              "id": "https://www.grid.ac/institutes/grid.470073.7", 
              "name": [
                "Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, 1981 Kraft Drive, 24061-0913, Blacksburg, VA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Meng", 
            "givenName": "Xiang-Jin", 
            "id": "sg:person.0727130151.68", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727130151.68"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/j.ebiom.2016.07.039", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003182606"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1473-3099(08)70255-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005992888"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.vetmic.2009.03.017", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008621650"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jvi.01475-10", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010998514"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jvi.00146-12", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011209822"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/jmv.20565", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011381837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/jmv.20565", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011381837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/cid/cit291", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013610468"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jvi.02259-06", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022521275"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.clinre.2014.07.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030163785"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.ppat.1005695", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030927748"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/hep.25505", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037998204"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jvi.01854-08", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038727803"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jvi.78.9.4838-4846.2004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042389255"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/emi.2012.7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043900386", 
              "https://doi.org/10.1038/emi.2012.7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.virusres.2011.01.016", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046806565"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/liv.12629", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049934244"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3201/eid1505.081100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053557356"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.hepres.2006.08.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1054736009"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/vir.0.043711-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060399058"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/vir.0.068429-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060399620"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/vir.0.18918-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060399819"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2018-12", 
        "datePublishedReg": "2018-12-01", 
        "description": "BACKGROUND: Genotype 3 hepatitis E virus (HEV) infection is generally associated with mild disease. However, recently eight genotype 3 HEV isolates were identified from patients with severe hepatitis. Importantly, three mutations (S605P, I978V and V1213A) in these genotype 3 isolates were found to be typical of genotype 4 HEV, which is sometime associated with more severe hepatitis. Therefore in this study we seek to determine if these unique mutations contribute to enhanced virus replication and thus potentially severe disease.\nMETHODS: In the lack of an efficient cell culture system to study the effect of mutations on HEV replication, we developed a genotype 3 HEV replicon with Renilla luciferase (Rluc) as reporter and subsequently used it to construct numerous mutants, including swMu-1 (V1213A), swMu-2 (Q1246H), swMu-3 (V1213A and Q1246H), swMu-4 (S605P and I978V), and swMu-5 (V1213A, S605P and I978V). RNA transcripts from mutant replicons were transfected into Huh7 S10-3 liver cells to measure the effect of mutations on HEV replication efficiency.\nRESULTS: The results showed that the V1213A mutant had the highest reduction in HEV replication efficiency than other mutants. The V1213A and S605P\u2009+\u2009I978V mutations have a cumulative, if not synergistic, effect on HEV replication. The Q1246H mutant decreased HEV replication compared to the wild-type HEV Rluc replicon but replicated better than the V1213A mutant. The amino acid residue V1213 favors the replication of both genotypes 3 and 4 HEV strains, but not genotype 1 HEV.\nCONCLUSION: The results suggested that the V1213A mutation reduced HEV replication, but is likely not associated with the reported severe hepatitis caused by genotype 3 HEV isolates containing this mutation.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1186/s12985-018-0943-5", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2455597", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2457986", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1034449", 
            "issn": [
              "1743-422X"
            ], 
            "name": "Virology Journal", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "15"
          }
        ], 
        "name": "Substitution of amino acid residue V1213 in the helicase domain of the genotype 3 hepatitis E virus reduces virus replication", 
        "pagination": "32", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "01051fe72d7e04480c4855fad8704700c0d53657740ea216cf539ec47fabc8c2"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "29422085"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101231645"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/s12985-018-0943-5"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1100913030"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/s12985-018-0943-5", 
          "https://app.dimensions.ai/details/publication/pub.1100913030"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T21:40", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000528.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1186/s12985-018-0943-5"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12985-018-0943-5'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12985-018-0943-5'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12985-018-0943-5'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12985-018-0943-5'


     

    This table displays all metadata directly associated to this object as RDF triples.

    194 TRIPLES      21 PREDICATES      61 URIs      32 LITERALS      20 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/s12985-018-0943-5 schema:about N10add7766eae4f2ebb41835dcb8918e4
    2 N2155ccdb9aee43e7a23d3964e6844132
    3 N69fdaad638d34d1fa3398172239669e4
    4 N7decb96706c143e5882e52b203cdfca2
    5 N89d44701a322452780730df14d124662
    6 Na40a0e60403044b78d202b3031c0012e
    7 Nda77274b2b544ef28dbf6330b4d041c1
    8 Ne756c05991a34baaade32211a487157e
    9 Ne819e70c20a443f3a3580573425a661b
    10 Nf2e218d867a54f2db41893995b5598a1
    11 Nfa80cbeb3b5a43d08ecb77a0804fb739
    12 anzsrc-for:11
    13 anzsrc-for:1108
    14 schema:author N60c5e414abfb4f1eb8474689589aa6a0
    15 schema:citation sg:pub.10.1038/emi.2012.7
    16 https://doi.org/10.1002/hep.25505
    17 https://doi.org/10.1002/jmv.20565
    18 https://doi.org/10.1016/j.clinre.2014.07.005
    19 https://doi.org/10.1016/j.ebiom.2016.07.039
    20 https://doi.org/10.1016/j.hepres.2006.08.002
    21 https://doi.org/10.1016/j.vetmic.2009.03.017
    22 https://doi.org/10.1016/j.virusres.2011.01.016
    23 https://doi.org/10.1016/s1473-3099(08)70255-x
    24 https://doi.org/10.1093/cid/cit291
    25 https://doi.org/10.1099/vir.0.043711-0
    26 https://doi.org/10.1099/vir.0.068429-0
    27 https://doi.org/10.1099/vir.0.18918-0
    28 https://doi.org/10.1111/liv.12629
    29 https://doi.org/10.1128/jvi.00146-12
    30 https://doi.org/10.1128/jvi.01475-10
    31 https://doi.org/10.1128/jvi.01854-08
    32 https://doi.org/10.1128/jvi.02259-06
    33 https://doi.org/10.1128/jvi.78.9.4838-4846.2004
    34 https://doi.org/10.1371/journal.ppat.1005695
    35 https://doi.org/10.3201/eid1505.081100
    36 schema:datePublished 2018-12
    37 schema:datePublishedReg 2018-12-01
    38 schema:description BACKGROUND: Genotype 3 hepatitis E virus (HEV) infection is generally associated with mild disease. However, recently eight genotype 3 HEV isolates were identified from patients with severe hepatitis. Importantly, three mutations (S605P, I978V and V1213A) in these genotype 3 isolates were found to be typical of genotype 4 HEV, which is sometime associated with more severe hepatitis. Therefore in this study we seek to determine if these unique mutations contribute to enhanced virus replication and thus potentially severe disease. METHODS: In the lack of an efficient cell culture system to study the effect of mutations on HEV replication, we developed a genotype 3 HEV replicon with Renilla luciferase (Rluc) as reporter and subsequently used it to construct numerous mutants, including swMu-1 (V1213A), swMu-2 (Q1246H), swMu-3 (V1213A and Q1246H), swMu-4 (S605P and I978V), and swMu-5 (V1213A, S605P and I978V). RNA transcripts from mutant replicons were transfected into Huh7 S10-3 liver cells to measure the effect of mutations on HEV replication efficiency. RESULTS: The results showed that the V1213A mutant had the highest reduction in HEV replication efficiency than other mutants. The V1213A and S605P + I978V mutations have a cumulative, if not synergistic, effect on HEV replication. The Q1246H mutant decreased HEV replication compared to the wild-type HEV Rluc replicon but replicated better than the V1213A mutant. The amino acid residue V1213 favors the replication of both genotypes 3 and 4 HEV strains, but not genotype 1 HEV. CONCLUSION: The results suggested that the V1213A mutation reduced HEV replication, but is likely not associated with the reported severe hepatitis caused by genotype 3 HEV isolates containing this mutation.
    39 schema:genre research_article
    40 schema:inLanguage en
    41 schema:isAccessibleForFree true
    42 schema:isPartOf Nf92fbe374f74466aaabeab0f0b8c0e63
    43 Nfe0419e22e924fb3aac20729838c84e7
    44 sg:journal.1034449
    45 schema:name Substitution of amino acid residue V1213 in the helicase domain of the genotype 3 hepatitis E virus reduces virus replication
    46 schema:pagination 32
    47 schema:productId N1bd6e5aa6d364df6b4ee1ba0f3989e7e
    48 N32fd95df04964c79a4e25b945dbbe823
    49 N506678bcdef84b68b51ecbf0b8bf29ab
    50 N619ee8865e0143b48d4e361b30348186
    51 N89a5d6e749164f25a0e744da8b163ec6
    52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100913030
    53 https://doi.org/10.1186/s12985-018-0943-5
    54 schema:sdDatePublished 2019-04-10T21:40
    55 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    56 schema:sdPublisher N442c37803caa4b0c873da4861c8d3128
    57 schema:url http://link.springer.com/10.1186/s12985-018-0943-5
    58 sgo:license sg:explorer/license/
    59 sgo:sdDataset articles
    60 rdf:type schema:ScholarlyArticle
    61 N10add7766eae4f2ebb41835dcb8918e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    62 schema:name Virus Replication
    63 rdf:type schema:DefinedTerm
    64 N1bd6e5aa6d364df6b4ee1ba0f3989e7e schema:name readcube_id
    65 schema:value 01051fe72d7e04480c4855fad8704700c0d53657740ea216cf539ec47fabc8c2
    66 rdf:type schema:PropertyValue
    67 N2155ccdb9aee43e7a23d3964e6844132 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    68 schema:name Genes, Reporter
    69 rdf:type schema:DefinedTerm
    70 N32fd95df04964c79a4e25b945dbbe823 schema:name pubmed_id
    71 schema:value 29422085
    72 rdf:type schema:PropertyValue
    73 N442c37803caa4b0c873da4861c8d3128 schema:name Springer Nature - SN SciGraph project
    74 rdf:type schema:Organization
    75 N506678bcdef84b68b51ecbf0b8bf29ab schema:name nlm_unique_id
    76 schema:value 101231645
    77 rdf:type schema:PropertyValue
    78 N573915051b214441aad8ce0de39fe7a3 rdf:first sg:person.0727130151.68
    79 rdf:rest rdf:nil
    80 N60c5e414abfb4f1eb8474689589aa6a0 rdf:first sg:person.0640527462.71
    81 rdf:rest Nf3cf6ce7f9d44df9a25e1877ed9f4626
    82 N619ee8865e0143b48d4e361b30348186 schema:name dimensions_id
    83 schema:value pub.1100913030
    84 rdf:type schema:PropertyValue
    85 N69fdaad638d34d1fa3398172239669e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    86 schema:name Viral Proteins
    87 rdf:type schema:DefinedTerm
    88 N7decb96706c143e5882e52b203cdfca2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    89 schema:name Genotype
    90 rdf:type schema:DefinedTerm
    91 N89a5d6e749164f25a0e744da8b163ec6 schema:name doi
    92 schema:value 10.1186/s12985-018-0943-5
    93 rdf:type schema:PropertyValue
    94 N89d44701a322452780730df14d124662 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    95 schema:name Hepatitis E
    96 rdf:type schema:DefinedTerm
    97 Na40a0e60403044b78d202b3031c0012e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    98 schema:name Hepatitis E virus
    99 rdf:type schema:DefinedTerm
    100 Nda77274b2b544ef28dbf6330b4d041c1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    101 schema:name Gene Expression
    102 rdf:type schema:DefinedTerm
    103 Ne756c05991a34baaade32211a487157e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Amino Acid Substitution
    105 rdf:type schema:DefinedTerm
    106 Ne819e70c20a443f3a3580573425a661b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Humans
    108 rdf:type schema:DefinedTerm
    109 Nf2e218d867a54f2db41893995b5598a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Cell Line
    111 rdf:type schema:DefinedTerm
    112 Nf3cf6ce7f9d44df9a25e1877ed9f4626 rdf:first sg:person.0733152350.42
    113 rdf:rest N573915051b214441aad8ce0de39fe7a3
    114 Nf92fbe374f74466aaabeab0f0b8c0e63 schema:issueNumber 1
    115 rdf:type schema:PublicationIssue
    116 Nfa80cbeb3b5a43d08ecb77a0804fb739 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name Mutation
    118 rdf:type schema:DefinedTerm
    119 Nfe0419e22e924fb3aac20729838c84e7 schema:volumeNumber 15
    120 rdf:type schema:PublicationVolume
    121 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    122 schema:name Medical and Health Sciences
    123 rdf:type schema:DefinedTerm
    124 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
    125 schema:name Medical Microbiology
    126 rdf:type schema:DefinedTerm
    127 sg:grant.2455597 http://pending.schema.org/fundedItem sg:pub.10.1186/s12985-018-0943-5
    128 rdf:type schema:MonetaryGrant
    129 sg:grant.2457986 http://pending.schema.org/fundedItem sg:pub.10.1186/s12985-018-0943-5
    130 rdf:type schema:MonetaryGrant
    131 sg:journal.1034449 schema:issn 1743-422X
    132 schema:name Virology Journal
    133 rdf:type schema:Periodical
    134 sg:person.0640527462.71 schema:affiliation https://www.grid.ac/institutes/grid.470073.7
    135 schema:familyName Cao
    136 schema:givenName Dianjun
    137 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640527462.71
    138 rdf:type schema:Person
    139 sg:person.0727130151.68 schema:affiliation https://www.grid.ac/institutes/grid.470073.7
    140 schema:familyName Meng
    141 schema:givenName Xiang-Jin
    142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727130151.68
    143 rdf:type schema:Person
    144 sg:person.0733152350.42 schema:affiliation https://www.grid.ac/institutes/grid.470073.7
    145 schema:familyName Ni
    146 schema:givenName Yan-Yan
    147 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733152350.42
    148 rdf:type schema:Person
    149 sg:pub.10.1038/emi.2012.7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043900386
    150 https://doi.org/10.1038/emi.2012.7
    151 rdf:type schema:CreativeWork
    152 https://doi.org/10.1002/hep.25505 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037998204
    153 rdf:type schema:CreativeWork
    154 https://doi.org/10.1002/jmv.20565 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011381837
    155 rdf:type schema:CreativeWork
    156 https://doi.org/10.1016/j.clinre.2014.07.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030163785
    157 rdf:type schema:CreativeWork
    158 https://doi.org/10.1016/j.ebiom.2016.07.039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003182606
    159 rdf:type schema:CreativeWork
    160 https://doi.org/10.1016/j.hepres.2006.08.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054736009
    161 rdf:type schema:CreativeWork
    162 https://doi.org/10.1016/j.vetmic.2009.03.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008621650
    163 rdf:type schema:CreativeWork
    164 https://doi.org/10.1016/j.virusres.2011.01.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046806565
    165 rdf:type schema:CreativeWork
    166 https://doi.org/10.1016/s1473-3099(08)70255-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005992888
    167 rdf:type schema:CreativeWork
    168 https://doi.org/10.1093/cid/cit291 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013610468
    169 rdf:type schema:CreativeWork
    170 https://doi.org/10.1099/vir.0.043711-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060399058
    171 rdf:type schema:CreativeWork
    172 https://doi.org/10.1099/vir.0.068429-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060399620
    173 rdf:type schema:CreativeWork
    174 https://doi.org/10.1099/vir.0.18918-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060399819
    175 rdf:type schema:CreativeWork
    176 https://doi.org/10.1111/liv.12629 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049934244
    177 rdf:type schema:CreativeWork
    178 https://doi.org/10.1128/jvi.00146-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011209822
    179 rdf:type schema:CreativeWork
    180 https://doi.org/10.1128/jvi.01475-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010998514
    181 rdf:type schema:CreativeWork
    182 https://doi.org/10.1128/jvi.01854-08 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038727803
    183 rdf:type schema:CreativeWork
    184 https://doi.org/10.1128/jvi.02259-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022521275
    185 rdf:type schema:CreativeWork
    186 https://doi.org/10.1128/jvi.78.9.4838-4846.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042389255
    187 rdf:type schema:CreativeWork
    188 https://doi.org/10.1371/journal.ppat.1005695 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030927748
    189 rdf:type schema:CreativeWork
    190 https://doi.org/10.3201/eid1505.081100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053557356
    191 rdf:type schema:CreativeWork
    192 https://www.grid.ac/institutes/grid.470073.7 schema:alternateName Virginia–Maryland College of Veterinary Medicine
    193 schema:name Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, 1981 Kraft Drive, 24061-0913, Blacksburg, VA, USA
    194 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...