Real-time reverse transcription PCR-based sequencing-independent pathotyping of Eurasian avian influenza A viruses of subtype H7 View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-07-24

AUTHORS

Annika Graaf, Martin Beer, Timm Harder

ABSTRACT

Low pathogenic avian influenza viruses (LPAIV) of the subtypes H5 and H7 are known to give rise to highly pathogenic (HP) phenotypes by spontaneous insertional mutations which convert a monobasic trypsin-sensitive endoproteolytical cleavage site (CS) within the hemagglutinin (HA) protein into a polybasic subtilisin-sensitive one. Sporadic outbreaks of notifiable LPAIV H7 infections are continuously recorded in Europe and in Asia, and some lineages showed zoonotic transmission. De novo generation of HPAIV H7 from LPAIV precursors has been reported several times over the past decade. Rapid differentiation between LP and HP H7 virus strains is required as a prerequisite to emplace appropriate control measures. Here, reverse transcription real-time PCR assays (RT-qPCR) were developed and evaluated that allow LP and HP pathotype identification and distinction by probe-assisted detection of the HACS. These new RT-qPCRs allow a sensitive and highly specific pathotype identification of Eurasian subtype H7 AIV in allantoic fluids as well as in diagnostic field samples. RT-qPCR assisted pathotyping presents a rapid and sensitive alternative to pathotyping by animal inoculation or nucleotide sequencing. More... »

PAGES

137

References to SciGraph publications

  • 2008. A Brief Introduction to the Avian Influenza Virus in AVIAN INFLUENZA VIRUS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12985-017-0808-3

    DOI

    http://dx.doi.org/10.1186/s12985-017-0808-3

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1090885775

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/28738896


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