Repression of HIV-1 reactivation mediated by CRISPR/dCas9-KRAB in lymphoid and myeloid cell models View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2022-06-22

AUTHORS

Lendel Correia da Costa, Larissa Maciel Bomfim, Uilla Victoria Torres Dittz, Camila de Almeida Velozo, Rodrigo Delvecchio da Cunha, Amilcar Tanuri

ABSTRACT

BackgroundDespite antiretroviral treatment efficacy, it does not lead to the complete eradication of HIV infection. Consequently, reactivation of the virus from latently infected cell reservoirs is a major challenge toward cure efforts. Two strategies targeting viral latency are currently under investigation: the “shock and kill” and the “block and lock.” The “Block and Lock” methodology aims to control HIV-1 latency reactivation, promoting a functional cure. We utilized the CRISPR/dCas9-KRAB platform, which was initially developed to suppress cellular genes transcription, to block drug-induced HIV-1 reactivation in latently infected T cells and myeloid cells.ResultsWe identified a set of five sgRNAs targeting the HIV-1 proviral genome (LTR1-LTR5), having the lowest nominated off-target activity, and transduced them into the latently infected lymphoid (J-Lat 10.6) and myeloid (U1) cell lines. One of the sgRNAs (LTR5), which binds specifically in the HIV-1 LTR NFκB binding site, was able to promote robust repression of HIV-1 reactivation in latently infected T cells stimulated with Phorbol 12-Myristate 13-Acetate (PMA) and Ingenol B (IngB), both potent protein kinase C (PKC) stimulators. Reactivation with HDAC inhibitors, such as SAHA and Panobinostat, showed the same strong inhibition of reactivation. Additionally, we observed a hundred times reduction of HIV-1 RNA expression levels in the latently infected myeloid cell line, U1 induced with IngB.ConclusionTaken together, our results show that the KRAB fused CRISPR/dCas9 system can robustly prevent the HIV-1 latency reactivation process, mediated by PMA or IngB and SAHA or Panobinostat, both in myeloid and lymphoid HIV-1 latently infected cells. In addition, we demonstrated that KRAB repressor protein is crucial to reactivation resistance phenotype, and we have identified some useful hotspots sequences in HIV-1 LTR for the design sgRNAs. More... »

PAGES

12

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12977-022-00600-9

DOI

http://dx.doi.org/10.1186/s12977-022-00600-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1148859130

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/35733180


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103 robust repression
104 sequence
105 set
106 sgRNA
107 sgRNAs
108 shock
109 sites
110 stimulator
111 strategies
112 strong inhibition
113 system
114 target activity
115 time reduction
116 transcription
117 treatment efficacy
118 viral latency
119 virus
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