Anti-inflammatory effects of recreational marijuana in virally suppressed youth with HIV-1 are reversed by use of tobacco products in combination ... View Full Text


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Article Info

DATE

2022-05-31

AUTHORS

Li Yin, Ashok R. Dinasarapu, Samiksha A. Borkar, Kai-Fen Chang, Kristina De Paris, Julie J. Kim-Chang, John W. Sleasman, Maureen M. Goodenow

ABSTRACT

BackgroundMarijuana’s putative anti-inflammatory properties may benefit HIV-associated comorbidities. How recreational marijuana use affects gene expression in peripheral blood cells (PBC) among youth with HIV-1 (YWH) is unknown.ApproachYWH with defined substance use (n = 54) receiving similar antiretroviral therapy (ART) were assigned to one of four analysis groups: YWH with detectable plasma HIV-1 (> 50 RNA copies/ml) who did not use substances (H+V+S−), and YWH with undetectable plasma HIV-1 who did not use substances (H+V−S−), or used marijuana alone (H+V−S+[M]), or marijuana in combination with tobacco (H+V−S+[M/T]). Non-substance using youth without HIV infection (H−S−, n = 25) provided a reference group. PBC mRNA was profiled by Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. Differentially expressed genes (DEG) within outcome groups were identified by Significance Analysis of Microarrays and used for Hierarchical Clustering, Principal Component Analysis, and Ingenuity Pathways Analysis.ResultsHIV-1 replication resulted in > 3000 DEG involving 27 perturbed pathways. Viral suppression reduced DEG to 313, normalized all 27 pathways, and down-regulated two additional pathways, while marijuana use among virally suppressed YWH resulted in 434 DEG and no perturbed pathways. Relative to H+V−S−, multiple DEG normalized in H+V−S+[M]. In contrast, H+V−S+[M/T] had 1140 DEG and 10 dysregulated pathways, including multiple proinflammatory genes and six pathways shared by H+V+S−.ConclusionsYWH receiving ART display unique transcriptome bioprofiles based on viral replication and substance use. In the context of HIV suppression, marijuana use, alone or combined with tobacco, has opposing effects on inflammatory gene expression. More... »

PAGES

10

References to SciGraph publications

  • 2018-07-30. GPR55 signalling promotes proliferation of pancreatic cancer cells and tumour growth in mice, and its inhibition increases effects of gemcitabine in ONCOGENE
  • 2014-02-23. Δ9-Tetrahydrocannabinol Treatment During Human Monocyte Differentiation Reduces Macrophage Susceptibility to HIV-1 Infection in JOURNAL OF NEUROIMMUNE PHARMACOLOGY
  • 2018-08-13. Mechanisms of signalling and biased agonism in G protein-coupled receptors in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2006-03-02. A simple method for assessing sample sizes in microarray experiments in BMC BIOINFORMATICS
  • 2011-12-20. Gene Array Studies in HIV-1 Infection in CURRENT HIV/AIDS REPORTS
  • 2017-02-14. Gene expression profiles associated with cigarette smoking and moist snuff consumption in BMC GENOMICS
  • 2004-11-08. Sample size for detecting differentially expressed genes in microarray experiments in BMC GENOMICS
  • 2015-10-13. Antiretroviral treatment initiation does not differentially alter neurocognitive functioning over time in youth with behaviorally acquired HIV in JOURNAL OF NEUROVIROLOGY
  • 2015-12-10. Identification of novel HIV-1 dependency factors in primary CCR4+CCR6+Th17 cells via a genome-wide transcriptional approach in RETROVIROLOGY
  • 2012-04-18. HIV and Inflammation: Mechanisms and Consequences in CURRENT HIV/AIDS REPORTS
  • 2005-05. Cannabinoid-based drugs as anti-inflammatory therapeutics in NATURE REVIEWS IMMUNOLOGY
  • 2015. Endocannabinoids and the Immune System in Health and Disease in ENDOCANNABINOIDS
  • 1998. The Role of Macrophages in THC-Induced Alteration of the Cytokine Network in DRUGS OF ABUSE, IMMUNOMODULATION, AND AIDS
  • 2013-12-27. Transcriptome analysis of primary monocytes from HIV-positive patients with differential responses to antiretroviral therapy in VIROLOGY JOURNAL
  • 2019-11-28. Transcriptomic analysis of monocytes from HIV-positive men on antiretroviral therapy reveals effects of tobacco smoking on interferon and stress response systems associated with depressive symptoms in HUMAN GENOMICS
  • 1998-03. Induction of HIV-1 Replication by Type 1-Like Cytokines, Interleukin (IL)-12 and IL-15: Effect on Viral Transcriptional Activation, Cellular Proliferation, and Endogenous Cytokine Production in JOURNAL OF CLINICAL IMMUNOLOGY
  • 2008-03. What is principal component analysis? in NATURE BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12977-022-00594-4

    DOI

    http://dx.doi.org/10.1186/s12977-022-00594-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1148265194

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/35642061


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    30 schema:description BackgroundMarijuana’s putative anti-inflammatory properties may benefit HIV-associated comorbidities. How recreational marijuana use affects gene expression in peripheral blood cells (PBC) among youth with HIV-1 (YWH) is unknown.ApproachYWH with defined substance use (n = 54) receiving similar antiretroviral therapy (ART) were assigned to one of four analysis groups: YWH with detectable plasma HIV-1 (> 50 RNA copies/ml) who did not use substances (H+V+S−), and YWH with undetectable plasma HIV-1 who did not use substances (H+V−S−), or used marijuana alone (H+V−S+[M]), or marijuana in combination with tobacco (H+V−S+[M/T]). Non-substance using youth without HIV infection (H−S−, n = 25) provided a reference group. PBC mRNA was profiled by Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. Differentially expressed genes (DEG) within outcome groups were identified by Significance Analysis of Microarrays and used for Hierarchical Clustering, Principal Component Analysis, and Ingenuity Pathways Analysis.ResultsHIV-1 replication resulted in > 3000 DEG involving 27 perturbed pathways. Viral suppression reduced DEG to 313, normalized all 27 pathways, and down-regulated two additional pathways, while marijuana use among virally suppressed YWH resulted in 434 DEG and no perturbed pathways. Relative to H+V−S−, multiple DEG normalized in H+V−S+[M]. In contrast, H+V−S+[M/T] had 1140 DEG and 10 dysregulated pathways, including multiple proinflammatory genes and six pathways shared by H+V+S−.ConclusionsYWH receiving ART display unique transcriptome bioprofiles based on viral replication and substance use. In the context of HIV suppression, marijuana use, alone or combined with tobacco, has opposing effects on inflammatory gene expression.
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