Trim28 acts as restriction factor of prototype foamy virus replication by modulating H3K9me3 marks and destabilizing the viral transactivator Tas View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2021-12-13

AUTHORS

Peipei Yuan, Jun Yan, Shuang Wang, Yang Guo, Xueyan Xi, Song Han, Jun Yin, Biwen Peng, Xiaohua He, Jochen Bodem, Wanhong Liu

ABSTRACT

BackgroundPrototype foamy virus (PFV) is nonpathogenic complex retroviruses that express a transcriptional transactivator Tas, which is essential for the activity of viral long terminal repeat (LTR) promoter and internal promoter (IP). Tripartite motif-containing protein 28 (Trim28) is well known as a scaffold protein normally enriched in gene promoter region to repress transcription. We sought to determine if whether Trim28 could be enriched in PFV promoter region to participate the establishment of PFV latency infection.ResultsIn this study, we show that Trim28 restricts Tas-dependent transactivation activity of PFV promoter and negatively regulates PFV replication. Trim28 was found to be enriched in LTR instead of IP promoter regions of PFV genome and contribute to the maintenance of histone H3K9me3 marks on the LTR promoter. Furthermore, Trim28 interacts with Tas and colocalizes with Tas in the nucleus. Besides, we found that Trim28, an E3 ubiquitin ligase, binds directly to and promotes Tas for ubiquitination and degradation. And the RBCC domain of Trim28 is required for the ubiquitination and degradation of Tas.ConclusionsCollectively, our findings not only identify a host factor Trim28 negatively inhibits PFV replication by acting as transcriptional restriction factor enriched in viral LTR promoter through modulating H3K9me3 mark here, but also reveal that Trim28 mediated ubiquitin proteasome degradation of Tas as a mechanism underlying Trim28 restricts Tas-dependent transcription activity of PFV promoter and PFV replication. These findings provide new insights into the process of PFV latency establishment.Graphical Abstract More... »

PAGES

38

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12977-021-00584-y

DOI

http://dx.doi.org/10.1186/s12977-021-00584-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1143843927

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/34903241


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Histones", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spumavirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Terminal Repeat Sequences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trans-Activators", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tripartite Motif-Containing Protein 28", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virus Replication", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of Medicine, 442000, Shiyan, Hubei, China", 
          "id": "http://www.grid.ac/institutes/grid.443573.2", 
          "name": [
            "Department of Immunology, School of Basic Medical Sciences, Hubei University of Medicine, 442000, Shiyan, China", 
            "Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, No. 185, Donghu Road, Wuchang District, 430071, Wuhan, China", 
            "Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of Medicine, 442000, Shiyan, Hubei, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yuan", 
        "givenName": "Peipei", 
        "id": "sg:person.010310470205.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010310470205.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China", 
          "id": "http://www.grid.ac/institutes/grid.49470.3e", 
          "name": [
            "Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, No. 185, Donghu Road, Wuchang District, 430071, Wuhan, China", 
            "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yan", 
        "givenName": "Jun", 
        "id": "sg:person.016006464253.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016006464253.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China", 
          "id": "http://www.grid.ac/institutes/grid.49470.3e", 
          "name": [
            "Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, No. 185, Donghu Road, Wuchang District, 430071, Wuhan, China", 
            "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Shuang", 
        "id": "sg:person.011106050605.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011106050605.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of Medicine, 442000, Shiyan, Hubei, China", 
          "id": "http://www.grid.ac/institutes/grid.443573.2", 
          "name": [
            "Department of Immunology, School of Basic Medical Sciences, Hubei University of Medicine, 442000, Shiyan, China", 
            "Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of Medicine, 442000, Shiyan, Hubei, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Guo", 
        "givenName": "Yang", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of Medicine, 442000, Shiyan, Hubei, China", 
          "id": "http://www.grid.ac/institutes/grid.443573.2", 
          "name": [
            "Department of Immunology, School of Basic Medical Sciences, Hubei University of Medicine, 442000, Shiyan, China", 
            "Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of Medicine, 442000, Shiyan, Hubei, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xi", 
        "givenName": "Xueyan", 
        "id": "sg:person.0774406236.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0774406236.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China", 
          "id": "http://www.grid.ac/institutes/grid.49470.3e", 
          "name": [
            "Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, No. 185, Donghu Road, Wuchang District, 430071, Wuhan, China", 
            "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Han", 
        "givenName": "Song", 
        "id": "sg:person.01213247430.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01213247430.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China", 
          "id": "http://www.grid.ac/institutes/grid.49470.3e", 
          "name": [
            "Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, No. 185, Donghu Road, Wuchang District, 430071, Wuhan, China", 
            "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yin", 
        "givenName": "Jun", 
        "id": "sg:person.01130714452.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130714452.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China", 
          "id": "http://www.grid.ac/institutes/grid.49470.3e", 
          "name": [
            "Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, No. 185, Donghu Road, Wuchang District, 430071, Wuhan, China", 
            "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Peng", 
        "givenName": "Biwen", 
        "id": "sg:person.01327476030.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327476030.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China", 
          "id": "http://www.grid.ac/institutes/grid.49470.3e", 
          "name": [
            "Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, No. 185, Donghu Road, Wuchang District, 430071, Wuhan, China", 
            "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "He", 
        "givenName": "Xiaohua", 
        "id": "sg:person.0652205130.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652205130.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut f\u00fcr Virologie und Immunbiologie, Julius-Maximilians-Universit\u00e4t W\u00fcrzburg, 97078, W\u00fcrzburg, Germany", 
          "id": "http://www.grid.ac/institutes/grid.8379.5", 
          "name": [
            "Institut f\u00fcr Virologie und Immunbiologie, Julius-Maximilians-Universit\u00e4t W\u00fcrzburg, 97078, W\u00fcrzburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bodem", 
        "givenName": "Jochen", 
        "id": "sg:person.01322034213.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322034213.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China", 
          "id": "http://www.grid.ac/institutes/grid.49470.3e", 
          "name": [
            "Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, No. 185, Donghu Road, Wuchang District, 430071, Wuhan, China", 
            "Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Wanhong", 
        "id": "sg:person.01177027652.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177027652.93"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/s41598-021-82164-w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1134955401", 
          "https://doi.org/10.1038/s41598-021-82164-w"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00430-010-0158-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040523728", 
          "https://doi.org/10.1007/s00430-010-0158-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri2413", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051808948", 
          "https://doi.org/10.1038/nri2413"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08674", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026444453", 
          "https://doi.org/10.1038/nature08674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12977-017-0341-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084251556", 
          "https://doi.org/10.1186/s12977-017-0341-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41418-020-00701-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1133539876", 
          "https://doi.org/10.1038/s41418-020-00701-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07844", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043011516", 
          "https://doi.org/10.1038/nature07844"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2199-5-16", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018556451", 
          "https://doi.org/10.1186/1471-2199-5-16"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2021-12-13", 
    "datePublishedReg": "2021-12-13", 
    "description": "BackgroundPrototype foamy virus (PFV) is nonpathogenic complex retroviruses that express a transcriptional transactivator Tas, which is essential for the activity of viral long terminal repeat (LTR) promoter and internal promoter (IP). Tripartite motif-containing protein 28 (Trim28) is well known as a scaffold protein normally enriched in gene promoter region to repress transcription. We sought to determine if\u00a0whether\u00a0Trim28 could be enriched in PFV promoter region to participate the establishment of PFV latency infection.ResultsIn this study, we show that Trim28 restricts Tas-dependent transactivation activity of PFV promoter and negatively regulates PFV replication. Trim28 was found to be enriched in LTR instead of IP promoter regions of PFV genome and contribute to the maintenance of histone H3K9me3 marks on the LTR promoter. Furthermore, Trim28 interacts with Tas and colocalizes with Tas in\u00a0the\u00a0nucleus. Besides, we found that Trim28, an E3 ubiquitin ligase, binds directly to and promotes Tas for ubiquitination and degradation. And the RBCC domain of Trim28 is required for the\u00a0ubiquitination\u00a0and\u00a0degradation\u00a0of\u00a0Tas.ConclusionsCollectively, our findings not only identify a host factor Trim28 negatively inhibits PFV replication by acting as transcriptional restriction factor enriched in viral LTR promoter through modulating H3K9me3 mark here, but also reveal that Trim28 mediated ubiquitin proteasome degradation of Tas as a mechanism underlying Trim28 restricts Tas-dependent transcription activity of PFV promoter and PFV replication. These findings provide new insights into the process of PFV latency establishment.Graphical Abstract", 
    "genre": "article", 
    "id": "sg:pub.10.1186/s12977-021-00584-y", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.8333218", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1033800", 
        "issn": [
          "1742-4690"
        ], 
        "name": "Retrovirology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "18"
      }
    ], 
    "keywords": [
      "H3K9me3 marks", 
      "PFV replication", 
      "promoter region", 
      "internal promoter", 
      "viral long terminal repeat (LTR) promoter", 
      "LTR promoter", 
      "E3 ubiquitin ligase", 
      "long terminal repeat promoter", 
      "tripartite motif-containing protein 28", 
      "restriction factors", 
      "gene promoter region", 
      "viral LTR promoter", 
      "scaffold protein", 
      "ubiquitin ligase", 
      "RBCC domain", 
      "transactivation activity", 
      "TRIM28", 
      "transcription activity", 
      "protein 28", 
      "complex retroviruses", 
      "repeat promoter", 
      "promoter", 
      "latency infection", 
      "ubiquitination", 
      "replication", 
      "new insights", 
      "virus replication", 
      "latency establishment", 
      "genome", 
      "ligase", 
      "transcription", 
      "ubiquitin", 
      "degradation", 
      "marks", 
      "protein", 
      "binds", 
      "LTR", 
      "establishment", 
      "retroviruses", 
      "activity", 
      "region", 
      "domain", 
      "maintenance", 
      "nucleus", 
      "virus", 
      "insights", 
      "mechanism", 
      "factors", 
      "findings", 
      "infection", 
      "process", 
      "study", 
      "Ta", 
      "ResultsIn", 
      "prototype"
    ], 
    "name": "Trim28 acts as restriction factor of prototype foamy virus replication by modulating H3K9me3 marks and destabilizing the viral transactivator Tas", 
    "pagination": "38", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1143843927"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s12977-021-00584-y"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "34903241"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s12977-021-00584-y", 
      "https://app.dimensions.ai/details/publication/pub.1143843927"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-10-01T06:49", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_890.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1186/s12977-021-00584-y"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12977-021-00584-y'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12977-021-00584-y'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12977-021-00584-y'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12977-021-00584-y'


 

This table displays all metadata directly associated to this object as RDF triples.

259 TRIPLES      21 PREDICATES      96 URIs      80 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s12977-021-00584-y schema:about N0271a6541b5543738fb3f6d04bd7811f
2 N61ac82e2b684485386f8c0e0238b004f
3 N699cba16872442afb2c7764f7f88cc93
4 N74d8e2781e7a42968070eb9b27b1e81b
5 Nae7e64f0efd644dcb3863f5a5c8399ae
6 Nb26ad05250d74638b0aea4efee06c332
7 Nc20b15b37a9c48f0830ad75fcf31647b
8 Nee82553122014c2889fab09862a90d12
9 anzsrc-for:11
10 anzsrc-for:1108
11 schema:author Nc4bc246a86274f4a89b66f703560545e
12 schema:citation sg:pub.10.1007/s00430-010-0158-x
13 sg:pub.10.1038/nature07844
14 sg:pub.10.1038/nature08674
15 sg:pub.10.1038/nri2413
16 sg:pub.10.1038/s41418-020-00701-y
17 sg:pub.10.1038/s41598-021-82164-w
18 sg:pub.10.1186/1471-2199-5-16
19 sg:pub.10.1186/s12977-017-0341-x
20 schema:datePublished 2021-12-13
21 schema:datePublishedReg 2021-12-13
22 schema:description BackgroundPrototype foamy virus (PFV) is nonpathogenic complex retroviruses that express a transcriptional transactivator Tas, which is essential for the activity of viral long terminal repeat (LTR) promoter and internal promoter (IP). Tripartite motif-containing protein 28 (Trim28) is well known as a scaffold protein normally enriched in gene promoter region to repress transcription. We sought to determine if whether Trim28 could be enriched in PFV promoter region to participate the establishment of PFV latency infection.ResultsIn this study, we show that Trim28 restricts Tas-dependent transactivation activity of PFV promoter and negatively regulates PFV replication. Trim28 was found to be enriched in LTR instead of IP promoter regions of PFV genome and contribute to the maintenance of histone H3K9me3 marks on the LTR promoter. Furthermore, Trim28 interacts with Tas and colocalizes with Tas in the nucleus. Besides, we found that Trim28, an E3 ubiquitin ligase, binds directly to and promotes Tas for ubiquitination and degradation. And the RBCC domain of Trim28 is required for the ubiquitination and degradation of Tas.ConclusionsCollectively, our findings not only identify a host factor Trim28 negatively inhibits PFV replication by acting as transcriptional restriction factor enriched in viral LTR promoter through modulating H3K9me3 mark here, but also reveal that Trim28 mediated ubiquitin proteasome degradation of Tas as a mechanism underlying Trim28 restricts Tas-dependent transcription activity of PFV promoter and PFV replication. These findings provide new insights into the process of PFV latency establishment.Graphical Abstract
23 schema:genre article
24 schema:isAccessibleForFree true
25 schema:isPartOf N2fa6973fca3b416786caa68b711569d3
26 Nb990b1e8c7e74cbda74ef40868975f05
27 sg:journal.1033800
28 schema:keywords E3 ubiquitin ligase
29 H3K9me3 marks
30 LTR
31 LTR promoter
32 PFV replication
33 RBCC domain
34 ResultsIn
35 TRIM28
36 Ta
37 activity
38 binds
39 complex retroviruses
40 degradation
41 domain
42 establishment
43 factors
44 findings
45 gene promoter region
46 genome
47 infection
48 insights
49 internal promoter
50 latency establishment
51 latency infection
52 ligase
53 long terminal repeat promoter
54 maintenance
55 marks
56 mechanism
57 new insights
58 nucleus
59 process
60 promoter
61 promoter region
62 protein
63 protein 28
64 prototype
65 region
66 repeat promoter
67 replication
68 restriction factors
69 retroviruses
70 scaffold protein
71 study
72 transactivation activity
73 transcription
74 transcription activity
75 tripartite motif-containing protein 28
76 ubiquitin
77 ubiquitin ligase
78 ubiquitination
79 viral LTR promoter
80 viral long terminal repeat (LTR) promoter
81 virus
82 virus replication
83 schema:name Trim28 acts as restriction factor of prototype foamy virus replication by modulating H3K9me3 marks and destabilizing the viral transactivator Tas
84 schema:pagination 38
85 schema:productId N3de2570a09c34f3eb865bf8af1dae115
86 N472f931be94f44d49cc1fd0b6547dd37
87 N6bf8c267539f40f1a164657a9f53f640
88 schema:sameAs https://app.dimensions.ai/details/publication/pub.1143843927
89 https://doi.org/10.1186/s12977-021-00584-y
90 schema:sdDatePublished 2022-10-01T06:49
91 schema:sdLicense https://scigraph.springernature.com/explorer/license/
92 schema:sdPublisher N1183c4de41324c13bdc45daa8359592a
93 schema:url https://doi.org/10.1186/s12977-021-00584-y
94 sgo:license sg:explorer/license/
95 sgo:sdDataset articles
96 rdf:type schema:ScholarlyArticle
97 N0271a6541b5543738fb3f6d04bd7811f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Terminal Repeat Sequences
99 rdf:type schema:DefinedTerm
100 N1183c4de41324c13bdc45daa8359592a schema:name Springer Nature - SN SciGraph project
101 rdf:type schema:Organization
102 N17bf656f18164f2896972c151ab5bc15 rdf:first sg:person.0774406236.28
103 rdf:rest Nc679e8d8864a46488d7457ca9448b8a4
104 N2fa6973fca3b416786caa68b711569d3 schema:issueNumber 1
105 rdf:type schema:PublicationIssue
106 N35ad7f023fdc41eab459bbceca24528d rdf:first sg:person.01130714452.35
107 rdf:rest N75cfad1495b94f0f9a576f5a35438de9
108 N383649e075704c86896ae801ed581c2d rdf:first sg:person.0652205130.53
109 rdf:rest N8ad4fe86d1e54c568b9509a8d9256fb7
110 N3de2570a09c34f3eb865bf8af1dae115 schema:name doi
111 schema:value 10.1186/s12977-021-00584-y
112 rdf:type schema:PropertyValue
113 N472f931be94f44d49cc1fd0b6547dd37 schema:name dimensions_id
114 schema:value pub.1143843927
115 rdf:type schema:PropertyValue
116 N61ac82e2b684485386f8c0e0238b004f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Histones
118 rdf:type schema:DefinedTerm
119 N699cba16872442afb2c7764f7f88cc93 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Tripartite Motif-Containing Protein 28
121 rdf:type schema:DefinedTerm
122 N6bf8c267539f40f1a164657a9f53f640 schema:name pubmed_id
123 schema:value 34903241
124 rdf:type schema:PropertyValue
125 N74a2e7eccf1e4ddb8e2cf071c29e62f4 rdf:first sg:person.011106050605.31
126 rdf:rest Nf6bb6d6c0aae432e8065673dc2b426b4
127 N74d8e2781e7a42968070eb9b27b1e81b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Humans
129 rdf:type schema:DefinedTerm
130 N75cfad1495b94f0f9a576f5a35438de9 rdf:first sg:person.01327476030.18
131 rdf:rest N383649e075704c86896ae801ed581c2d
132 N8a85bc3b34ed44cb8a6dae7a6039c891 rdf:first sg:person.01177027652.93
133 rdf:rest rdf:nil
134 N8ad4fe86d1e54c568b9509a8d9256fb7 rdf:first sg:person.01322034213.24
135 rdf:rest N8a85bc3b34ed44cb8a6dae7a6039c891
136 Nae7e64f0efd644dcb3863f5a5c8399ae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Virus Replication
138 rdf:type schema:DefinedTerm
139 Nb1365362dc784c55bcb62853eedf2c78 rdf:first sg:person.016006464253.11
140 rdf:rest N74a2e7eccf1e4ddb8e2cf071c29e62f4
141 Nb26ad05250d74638b0aea4efee06c332 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Trans-Activators
143 rdf:type schema:DefinedTerm
144 Nb990b1e8c7e74cbda74ef40868975f05 schema:volumeNumber 18
145 rdf:type schema:PublicationVolume
146 Nc20b15b37a9c48f0830ad75fcf31647b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Cell Line
148 rdf:type schema:DefinedTerm
149 Nc43d915a51a54bc7a99b52b8f24a42b0 schema:affiliation grid-institutes:grid.443573.2
150 schema:familyName Guo
151 schema:givenName Yang
152 rdf:type schema:Person
153 Nc4bc246a86274f4a89b66f703560545e rdf:first sg:person.010310470205.24
154 rdf:rest Nb1365362dc784c55bcb62853eedf2c78
155 Nc679e8d8864a46488d7457ca9448b8a4 rdf:first sg:person.01213247430.58
156 rdf:rest N35ad7f023fdc41eab459bbceca24528d
157 Nee82553122014c2889fab09862a90d12 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Spumavirus
159 rdf:type schema:DefinedTerm
160 Nf6bb6d6c0aae432e8065673dc2b426b4 rdf:first Nc43d915a51a54bc7a99b52b8f24a42b0
161 rdf:rest N17bf656f18164f2896972c151ab5bc15
162 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
163 schema:name Medical and Health Sciences
164 rdf:type schema:DefinedTerm
165 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
166 schema:name Medical Microbiology
167 rdf:type schema:DefinedTerm
168 sg:grant.8333218 http://pending.schema.org/fundedItem sg:pub.10.1186/s12977-021-00584-y
169 rdf:type schema:MonetaryGrant
170 sg:journal.1033800 schema:issn 1742-4690
171 schema:name Retrovirology
172 schema:publisher Springer Nature
173 rdf:type schema:Periodical
174 sg:person.010310470205.24 schema:affiliation grid-institutes:grid.443573.2
175 schema:familyName Yuan
176 schema:givenName Peipei
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010310470205.24
178 rdf:type schema:Person
179 sg:person.011106050605.31 schema:affiliation grid-institutes:grid.49470.3e
180 schema:familyName Wang
181 schema:givenName Shuang
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011106050605.31
183 rdf:type schema:Person
184 sg:person.01130714452.35 schema:affiliation grid-institutes:grid.49470.3e
185 schema:familyName Yin
186 schema:givenName Jun
187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130714452.35
188 rdf:type schema:Person
189 sg:person.01177027652.93 schema:affiliation grid-institutes:grid.49470.3e
190 schema:familyName Liu
191 schema:givenName Wanhong
192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177027652.93
193 rdf:type schema:Person
194 sg:person.01213247430.58 schema:affiliation grid-institutes:grid.49470.3e
195 schema:familyName Han
196 schema:givenName Song
197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01213247430.58
198 rdf:type schema:Person
199 sg:person.01322034213.24 schema:affiliation grid-institutes:grid.8379.5
200 schema:familyName Bodem
201 schema:givenName Jochen
202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322034213.24
203 rdf:type schema:Person
204 sg:person.01327476030.18 schema:affiliation grid-institutes:grid.49470.3e
205 schema:familyName Peng
206 schema:givenName Biwen
207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327476030.18
208 rdf:type schema:Person
209 sg:person.016006464253.11 schema:affiliation grid-institutes:grid.49470.3e
210 schema:familyName Yan
211 schema:givenName Jun
212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016006464253.11
213 rdf:type schema:Person
214 sg:person.0652205130.53 schema:affiliation grid-institutes:grid.49470.3e
215 schema:familyName He
216 schema:givenName Xiaohua
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652205130.53
218 rdf:type schema:Person
219 sg:person.0774406236.28 schema:affiliation grid-institutes:grid.443573.2
220 schema:familyName Xi
221 schema:givenName Xueyan
222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0774406236.28
223 rdf:type schema:Person
224 sg:pub.10.1007/s00430-010-0158-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1040523728
225 https://doi.org/10.1007/s00430-010-0158-x
226 rdf:type schema:CreativeWork
227 sg:pub.10.1038/nature07844 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043011516
228 https://doi.org/10.1038/nature07844
229 rdf:type schema:CreativeWork
230 sg:pub.10.1038/nature08674 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026444453
231 https://doi.org/10.1038/nature08674
232 rdf:type schema:CreativeWork
233 sg:pub.10.1038/nri2413 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051808948
234 https://doi.org/10.1038/nri2413
235 rdf:type schema:CreativeWork
236 sg:pub.10.1038/s41418-020-00701-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1133539876
237 https://doi.org/10.1038/s41418-020-00701-y
238 rdf:type schema:CreativeWork
239 sg:pub.10.1038/s41598-021-82164-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1134955401
240 https://doi.org/10.1038/s41598-021-82164-w
241 rdf:type schema:CreativeWork
242 sg:pub.10.1186/1471-2199-5-16 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018556451
243 https://doi.org/10.1186/1471-2199-5-16
244 rdf:type schema:CreativeWork
245 sg:pub.10.1186/s12977-017-0341-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1084251556
246 https://doi.org/10.1186/s12977-017-0341-x
247 rdf:type schema:CreativeWork
248 grid-institutes:grid.443573.2 schema:alternateName Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of Medicine, 442000, Shiyan, Hubei, China
249 schema:name Department of Immunology, School of Basic Medical Sciences, Hubei University of Medicine, 442000, Shiyan, China
250 Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of Medicine, 442000, Shiyan, Hubei, China
251 Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, No. 185, Donghu Road, Wuchang District, 430071, Wuhan, China
252 rdf:type schema:Organization
253 grid-institutes:grid.49470.3e schema:alternateName Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China
254 schema:name Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, No. 185, Donghu Road, Wuchang District, 430071, Wuhan, China
255 Hubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan University, 430071, Wuhan, China
256 rdf:type schema:Organization
257 grid-institutes:grid.8379.5 schema:alternateName Institut für Virologie und Immunbiologie, Julius-Maximilians-Universität Würzburg, 97078, Würzburg, Germany
258 schema:name Institut für Virologie und Immunbiologie, Julius-Maximilians-Universität Würzburg, 97078, Würzburg, Germany
259 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...