Astrocytic A1/A2 paradigm participates in glycogen mobilization mediated neuroprotection on reperfusion injury after ischemic stroke View Full Text


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Article Info

DATE

2021-10-13

AUTHORS

Haiyun Guo, Ze Fan, Shiquan Wang, Lina Ma, Jin Wang, Doutong Yu, Zhen Zhang, Lin Wu, Zhengwu Peng, Wenming Liu, Wugang Hou, Yanhui Cai

ABSTRACT

BackgroundAstrocytic glycogen works as an essential energy reserve for surrounding neurons and is reported to accumulate excessively during cerebral ischemia/reperfusion (I/R) injury. Our previous study found that accumulated glycogen mobilization exhibits a neuroprotective effect against I/R damage. In addition, ischemia could transform astrocytes into A1-like (toxic) and A2-like (protective) subtypes. However, the underlying mechanism behind accumulated glycogen mobilization-mediated neuroprotection in cerebral reperfusion injury and its relationship with the astrocytic A1/A2 paradigm is unknown.MethodsAstrocytic glycogen phosphorylase, the rate-limiting enzyme in glycogen mobilization, was specifically overexpressed and knocked down in mice and in cultured astrocytes. The I/R injury was imitated using a middle cerebral artery occlusion/reperfusion model in mice and an oxygen–glucose deprivation/reoxygenation model in cultured cells. Alterations in A1-like and A2-like astrocytes and the expression of phosphorylated nuclear transcription factor-κB (NF-κB) and phosphorylated signal transducer and activator of transcription 3 (STAT3) were determined by RNA sequencing, immunofluorescence and immunoblotting. Metabolites, including glycogen, NADPH, glutathione and reactive oxygen species (ROS), were analyzed by biochemical analysis.ResultsHere, we observed that astrocytic glycogen mobilization inhibited A1-like astrocytes and enhanced A2-like astrocytes after reperfusion in an experimental ischemic stroke model in vivo and in vitro. In addition, glycogen mobilization could enhance the production of NADPH and glutathione by the pentose phosphate pathway (PPP) and reduce ROS levels during reperfusion. NF-κB inhibition and STAT3 activation caused by a decrease in ROS levels were responsible for glycogen mobilization-induced A1-like and A2-like astrocyte transformation after I/R. The astrocytic A1/A2 paradigm is closely correlated with glycogen mobilization-mediated neuroprotection in cerebral reperfusion injury.ConclusionsOur data suggest that ROS-mediated NF-κB inhibition and STAT3 activation are the key pathways for glycogen mobilization-induced neuroprotection and provide a promising metabolic target for brain reperfusion injury in ischemic stroke. More... »

PAGES

230

References to SciGraph publications

  • 2019-10-31. The Structure and the Regulation of Glycogen Phosphorylases in Brain in BRAIN GLYCOGEN METABOLISM
  • 2019-10-31. Regional Distribution of Glycogen in the Mouse Brain Visualized by Immunohistochemistry in BRAIN GLYCOGEN METABOLISM
  • 2016-03-30. Astrocyte scar formation aids central nervous system axon regeneration in NATURE
  • 2019-10-31. Major Advances in Brain Glycogen Research: Understanding of the Roles of Glycogen Have Evolved from Emergency Fuel Reserve to Dynamic, Regulated Participant in Diverse Brain Functions in BRAIN GLYCOGEN METABOLISM
  • 2019-10-31. Structure and Regulation of Glycogen Synthase in the Brain in BRAIN GLYCOGEN METABOLISM
  • 2017-05-25. Toll-Like Receptor 4 (TLR4) and Triggering Receptor Expressed on Myeloid Cells-2 (TREM-2) Activation Balance Astrocyte Polarization into a Proinflammatory Phenotype in MOLECULAR NEUROBIOLOGY
  • 2019-01-19. Effects of Treadmill Exercise on Motor and Cognitive Function Recovery of MCAO Mice Through the Caveolin-1/VEGF Signaling Pathway in Ischemic Penumbra in NEUROCHEMICAL RESEARCH
  • 2019-10-31. Glycogenolysis in Cerebral Cortex During Sensory Stimulation, Acute Hypoglycemia, and Exercise: Impact on Astrocytic Energetics, Aerobic Glycolysis, and Astrocyte-Neuron Interactions in BRAIN GLYCOGEN METABOLISM
  • 2018-11-17. Neuroprotective Mechanism of Hypoxic Post-conditioning Involves HIF1-Associated Regulation of the Pentose Phosphate Pathway in Rat Brain in NEUROCHEMICAL RESEARCH
  • 2014-11-16. Glycogen metabolism and the homeostatic regulation of sleep in METABOLIC BRAIN DISEASE
  • 2018-07-09. STAT3 on the brain in NATURE REVIEWS CANCER
  • 2021-02-15. Reactive astrocyte nomenclature, definitions, and future directions in NATURE NEUROSCIENCE
  • 2020-09-11. Cottonseed oil alleviates ischemic stroke injury by inhibiting the inflammatory activation of microglia and astrocyte in JOURNAL OF NEUROINFLAMMATION
  • 2006-06-18. Conditional ablation of Stat3 or Socs3 discloses a dual role for reactive astrocytes after spinal cord injury in NATURE MEDICINE
  • 2017-03-30. Mitochondrial complex I inhibition triggers a mitophagy-dependent ROS increase leading to necroptosis and ferroptosis in melanoma cells in CELL DEATH & DISEASE
  • 2017-01-18. Neurotoxic reactive astrocytes are induced by activated microglia in NATURE
  • 2018-07-12. The novel estrogenic receptor GPR30 alleviates ischemic injury by inhibiting TLR4-mediated microglial inflammation in JOURNAL OF NEUROINFLAMMATION
  • 2015-10-20. Targeting mitochondrial complex I using BAY 87-2243 reduces melanoma tumor growth in CANCER & METABOLISM
  • 2017-09-05. TREK-1 mediates isoflurane-induced cytotoxicity in astrocytes in BMC ANESTHESIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12974-021-02284-y

    DOI

    http://dx.doi.org/10.1186/s12974-021-02284-y

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1141858907

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/34645472


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        "description": "BackgroundAstrocytic glycogen works as an essential energy reserve for surrounding neurons and is reported to accumulate excessively during cerebral ischemia/reperfusion (I/R) injury. Our previous study found that accumulated glycogen mobilization exhibits a neuroprotective effect against I/R damage. In addition, ischemia could transform astrocytes into A1-like (toxic) and A2-like (protective) subtypes. However, the underlying mechanism behind accumulated glycogen mobilization-mediated neuroprotection in cerebral reperfusion injury and its relationship with the astrocytic A1/A2 paradigm is unknown.MethodsAstrocytic glycogen phosphorylase, the rate-limiting enzyme in glycogen mobilization, was specifically overexpressed and knocked down in mice and in cultured astrocytes. The I/R injury was imitated using a middle cerebral artery occlusion/reperfusion model in mice and an oxygen\u2013glucose deprivation/reoxygenation model in cultured cells. Alterations in A1-like and A2-like astrocytes and the expression of phosphorylated nuclear transcription factor-\u03baB (NF-\u03baB) and phosphorylated signal transducer and activator of transcription 3 (STAT3) were determined by RNA sequencing, immunofluorescence and immunoblotting. Metabolites, including glycogen, NADPH, glutathione and reactive oxygen species (ROS), were analyzed by biochemical analysis.ResultsHere, we observed that astrocytic glycogen mobilization inhibited A1-like astrocytes and enhanced A2-like astrocytes after reperfusion in an experimental ischemic stroke model in vivo and in vitro. In addition, glycogen mobilization could enhance the production of NADPH and glutathione by the pentose phosphate pathway (PPP) and reduce ROS levels during reperfusion. NF-\u03baB inhibition and STAT3 activation caused by a decrease in ROS levels were responsible for glycogen mobilization-induced A1-like and A2-like astrocyte transformation after I/R. The astrocytic A1/A2 paradigm is closely correlated with glycogen mobilization-mediated neuroprotection in cerebral reperfusion injury.ConclusionsOur data suggest that ROS-mediated NF-\u03baB inhibition and STAT3 activation are the key pathways for glycogen mobilization-induced neuroprotection and provide a promising metabolic target for brain reperfusion injury in ischemic stroke.", 
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