Effects of oral phosphatidic acid feeding with or without whey protein on muscle protein synthesis and anabolic signaling in rodent ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-08-16

AUTHORS

C. Brooks Mobley, Troy A. Hornberger, Carlton D. Fox, James C. Healy, Brian S. Ferguson, Ryan P. Lowery, Rachel M. McNally, Christopher M. Lockwood, Jeffrey R. Stout, Andreas N. Kavazis, Jacob M. Wilson, Michael D. Roberts

ABSTRACT

BACKGROUND: Phosphatidic acid (PA) is a diacyl-glycerophospholipid that acts as a signaling molecule in numerous cellular processes. Recently, PA has been proposed to stimulate skeletal muscle protein accretion, but mechanistic studies are lacking. Furthermore, it is unknown whether co-ingesting PA with other leucine-containing ingredients can enhance intramuscular anabolic signaling mechanisms. Thus, the purpose of this study was to examine if oral PA feeding acutely increases anabolic signaling markers and muscle protein synthesis (MPS) in gastrocnemius with and without whey protein concentrate (WPC). METHODS: Overnight fasted male Wistar rats (~250 g) were randomly assigned to four groups: control (CON, n = 6-13), PA (29 mg; n = 8), WPC (197 mg; n = 8), or PA + WPC (n = 8). Three hours post-feeding, gastrocnemius muscle was removed for markers of Akt-mTOR signaling, gene expression patterns related to skeletal muscle mass regulation and metabolism, and MPS analysis via the SUnSET method. RESULTS: Compared to CON rats, PA, WPC and PA + WPC resulted in a significant elevation in the phosphorylation of mTOR (Ser2481) and rps6 (Ser235/236) (p < 0.05) in the gastrocnemius though there were no differences between the supplemented groups. MPS levels in the gastrocnemius were significantly (p < 0.05) elevated in WPC versus CON rats, and tended to be elevated in PA versus CON rats (p = 0.08), though MPS was less in PA + WPC versus WPC rats (p < 0.05) in spite of robust increases in mTOR pathway activity markers in the former group. C2C12 myoblast data agreed with the in vivo data herein showing that PA increased MPS levels 51% (p < 0.001) phosphorylated p70s6k (Thr389) levels 67% (p < 0.001). CONCLUSIONS: Our results are the first in vivo evidence to demonstrate that PA tends to increases MPS 3 h post-feeding, though PA may delay WPC-mediated MPS kinetics within a 3 h post-feeding window. More... »

PAGES

32

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12970-015-0094-7

DOI

http://dx.doi.org/10.1186/s12970-015-0094-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040094664

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26279644


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30 schema:description BACKGROUND: Phosphatidic acid (PA) is a diacyl-glycerophospholipid that acts as a signaling molecule in numerous cellular processes. Recently, PA has been proposed to stimulate skeletal muscle protein accretion, but mechanistic studies are lacking. Furthermore, it is unknown whether co-ingesting PA with other leucine-containing ingredients can enhance intramuscular anabolic signaling mechanisms. Thus, the purpose of this study was to examine if oral PA feeding acutely increases anabolic signaling markers and muscle protein synthesis (MPS) in gastrocnemius with and without whey protein concentrate (WPC). METHODS: Overnight fasted male Wistar rats (~250 g) were randomly assigned to four groups: control (CON, n = 6-13), PA (29 mg; n = 8), WPC (197 mg; n = 8), or PA + WPC (n = 8). Three hours post-feeding, gastrocnemius muscle was removed for markers of Akt-mTOR signaling, gene expression patterns related to skeletal muscle mass regulation and metabolism, and MPS analysis via the SUnSET method. RESULTS: Compared to CON rats, PA, WPC and PA + WPC resulted in a significant elevation in the phosphorylation of mTOR (Ser2481) and rps6 (Ser235/236) (p < 0.05) in the gastrocnemius though there were no differences between the supplemented groups. MPS levels in the gastrocnemius were significantly (p < 0.05) elevated in WPC versus CON rats, and tended to be elevated in PA versus CON rats (p = 0.08), though MPS was less in PA + WPC versus WPC rats (p < 0.05) in spite of robust increases in mTOR pathway activity markers in the former group. C2C12 myoblast data agreed with the in vivo data herein showing that PA increased MPS levels 51% (p < 0.001) phosphorylated p70s6k (Thr389) levels 67% (p < 0.001). CONCLUSIONS: Our results are the first in vivo evidence to demonstrate that PA tends to increases MPS 3 h post-feeding, though PA may delay WPC-mediated MPS kinetics within a 3 h post-feeding window.
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37 schema:keywords Akt-mTOR
38 C2C12 myoblast data
39 CON rats
40 MPS 3 h
41 MPS analysis
42 MPS kinetics
43 MPS levels
44 MPS levels 51
45 RPS6
46 SUnSET method
47 WPC rats
48 Wistar rats
49 accretion
50 acid
51 acid feeding
52 activity markers
53 anabolic
54 analysis
55 cellular processes
56 co-ingesting PA
57 concentrate
58 control
59 data
60 differences
61 effect
62 elevation
63 evidence
64 expression patterns
65 feeding
66 former group
67 gastrocnemius
68 gastrocnemius muscle
69 gene expression patterns
70 group
71 hours
72 increase
73 ingredients
74 intramuscular anabolic
75 kinetics
76 leucine-containing ingredients
77 levels
78 levels 51
79 mTOR
80 mTOR pathway activity markers
81 male Wistar rats
82 markers
83 mass regulation
84 mechanism
85 mechanistic studies
86 metabolism
87 method
88 molecules
89 muscle
90 muscle mass regulation
91 muscle protein accretion
92 muscle protein synthesis
93 myoblast data
94 numerous cellular processes
95 oral PA
96 oral phosphatidic acid feeding
97 pathway activity markers
98 patterns
99 phosphatidic acid
100 phosphatidic acid feeding
101 phosphorylation
102 phosphorylation of mTOR
103 post-feeding window
104 process
105 protein
106 protein accretion
107 protein concentrate
108 protein synthesis
109 purpose
110 rats
111 regulation
112 results
113 robust increase
114 rodent skeletal muscle
115 significant elevation
116 skeletal muscle
117 skeletal muscle mass regulation
118 skeletal muscle protein accretion
119 spite
120 study
121 synthesis
122 vivo data
123 vivo evidence
124 whey protein concentrate
125 whey proteins
126 window
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