Effects of protein type and composition on postprandial markers of skeletal muscle anabolism, adipose tissue lipolysis, and hypothalamic gene expression View Full Text


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Article Info

DATE

2015-03-13

AUTHORS

Christopher Brooks Mobley, Carlton D Fox, Brian S Ferguson, Corrie A Pascoe, James C Healy, Jeremy S McAdam, Christopher M Lockwood, Michael D Roberts

ABSTRACT

BACKGROUND: We examined the acute effects of different dietary protein sources (0.19 g, dissolved in 1 ml of water) on skeletal muscle, adipose tissue and hypothalamic satiety-related markers in fasted, male Wistar rats (~250 g). METHODS: Oral gavage treatments included: a) whey protein concentrate (WPC, n = 15); b) 70:30 hydrolyzed whey-to-hydrolyzed egg albumin (70 W/30E, n = 15); c) 50 W/50E (n = 15); d) 30 W/70E (n = 15); and e) 1 ml of water with no protein as a fasting control (CTL, n = 14). RESULTS: Skeletal muscle analyses revealed that compared to CTL: a) phosphorylated (p) markers of mTOR signaling [p-mTOR (Ser2481) and p-rps6 (Ser235/236)] were elevated 2-4-fold in all protein groups 90 min post-treatment (p < 0.05); b) WPC and 70 W/30E increased muscle protein synthesis (MPS) 104% and 74% 180 min post-treatment, respectively (p < 0.05); and c) 70 W/30E increased p-AMPKα (Thr172) 90 and 180-min post-treatment as well as PGC-1α mRNA 90 min post-treatment. Subcutaneous (SQ) and omental fat (OMAT) analyses revealed: a) 70 W/30 W increased SQ fat phosphorylated hormone-sensitive lipase [p-HSL (Ser563)] 3.1-fold versus CTL and a 1.9-4.4-fold change versus all other test proteins 180 min post-treatment (p < 0.05); and b) WPC, 70 W/30E and 50 W/50E increased OMAT p-HSL 3.8-6.5-fold 180 min post-treatment versus CTL (p < 0.05). 70 W/30E and 30 W/70E increased hypothalamic POMC mRNA 90 min post-treatment versus CTL rats suggesting a satiety-related response may have occurred in the former groups. However, there was a compensatory increase in orexigenic AGRP mRNA in the 70 W/30E group 90 min post-treatment versus CTL rats, and there was a compensatory increase in orexigenic NPY mRNA in the 30 W/70E group 90 min post-treatment versus CTL rats. CONCLUSIONS: Higher amounts of whey versus egg protein stimulate the greatest post-treatment anabolic skeletal muscle response, though test proteins with higher amounts of WPH more favorably affected post-treatment markers related to adipose tissue lipolysis. More... »

PAGES

14

References to SciGraph publications

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    http://scigraph.springernature.com/pub.10.1186/s12970-015-0076-9

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    http://dx.doi.org/10.1186/s12970-015-0076-9

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/25792976


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    21 schema:description BACKGROUND: We examined the acute effects of different dietary protein sources (0.19 g, dissolved in 1 ml of water) on skeletal muscle, adipose tissue and hypothalamic satiety-related markers in fasted, male Wistar rats (~250 g). METHODS: Oral gavage treatments included: a) whey protein concentrate (WPC, n = 15); b) 70:30 hydrolyzed whey-to-hydrolyzed egg albumin (70 W/30E, n = 15); c) 50 W/50E (n = 15); d) 30 W/70E (n = 15); and e) 1 ml of water with no protein as a fasting control (CTL, n = 14). RESULTS: Skeletal muscle analyses revealed that compared to CTL: a) phosphorylated (p) markers of mTOR signaling [p-mTOR (Ser2481) and p-rps6 (Ser235/236)] were elevated 2-4-fold in all protein groups 90 min post-treatment (p < 0.05); b) WPC and 70 W/30E increased muscle protein synthesis (MPS) 104% and 74% 180 min post-treatment, respectively (p < 0.05); and c) 70 W/30E increased p-AMPKα (Thr172) 90 and 180-min post-treatment as well as PGC-1α mRNA 90 min post-treatment. Subcutaneous (SQ) and omental fat (OMAT) analyses revealed: a) 70 W/30 W increased SQ fat phosphorylated hormone-sensitive lipase [p-HSL (Ser563)] 3.1-fold versus CTL and a 1.9-4.4-fold change versus all other test proteins 180 min post-treatment (p < 0.05); and b) WPC, 70 W/30E and 50 W/50E increased OMAT p-HSL 3.8-6.5-fold 180 min post-treatment versus CTL (p < 0.05). 70 W/30E and 30 W/70E increased hypothalamic POMC mRNA 90 min post-treatment versus CTL rats suggesting a satiety-related response may have occurred in the former groups. However, there was a compensatory increase in orexigenic AGRP mRNA in the 70 W/30E group 90 min post-treatment versus CTL rats, and there was a compensatory increase in orexigenic NPY mRNA in the 30 W/70E group 90 min post-treatment versus CTL rats. CONCLUSIONS: Higher amounts of whey versus egg protein stimulate the greatest post-treatment anabolic skeletal muscle response, though test proteins with higher amounts of WPH more favorably affected post-treatment markers related to adipose tissue lipolysis.
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    28 schema:keywords AMPKα (Thr172) 90
    29 CTL
    30 CTL rats
    31 HSL 3.8
    32 NPY mRNA
    33 OMAT p
    34 PGC-1α mRNA 90
    35 POMC mRNA 90
    36 SQ fat phosphorylated hormone-sensitive lipase [p-HSL (Ser563)] 3.1-fold
    37 W/30E
    38 W/30E group 90
    39 W/50E
    40 W/70E group 90
    41 WPC
    42 WPH
    43 Wistar rats
    44 acute effects
    45 adipose tissue lipolysis
    46 affected post-treatment markers
    47 albumin
    48 amount
    49 anabolic skeletal muscle response
    50 anabolism
    51 analysis
    52 changes
    53 compensatory increase
    54 composition
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    56 control
    57 dietary protein source
    58 different dietary protein sources
    59 effect
    60 egg albumin
    61 egg proteins
    62 expression
    63 fat analysis
    64 fat phosphorylated hormone-sensitive lipase [p-HSL (Ser563)] 3.1-fold
    65 former group
    66 gavage treatment
    67 gene expression
    68 greatest post-treatment anabolic skeletal muscle response
    69 group
    70 group 90
    71 high amounts
    72 hormone-sensitive lipase [p-HSL (Ser563)] 3.1-fold
    73 hypothalamic POMC mRNA 90
    74 hypothalamic gene expression
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    77 lipase [p-HSL (Ser563)] 3.1-fold
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    79 mRNA
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    81 mTOR
    82 male Wistar rats
    83 markers
    84 min
    85 muscle
    86 muscle anabolism
    87 muscle analysis
    88 muscle protein synthesis (MPS) 104
    89 muscle responses
    90 omental fat (OMAT) analyses
    91 oral gavage treatment
    92 orexigenic NPY mRNA
    93 phosphorylated (p) markers
    94 phosphorylated hormone-sensitive lipase [p-HSL (Ser563)] 3.1-fold
    95 post-treatment anabolic skeletal muscle response
    96 post-treatment markers
    97 postprandial markers
    98 protein
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    100 protein groups 90
    101 protein source
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    103 protein types
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    105 rats
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    108 satiety-related responses
    109 skeletal muscle
    110 skeletal muscle anabolism
    111 skeletal muscle analysis
    112 skeletal muscle response
    113 source
    114 synthesis (MPS) 104
    115 test proteins
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    117 tissue
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    123 schema:name Effects of protein type and composition on postprandial markers of skeletal muscle anabolism, adipose tissue lipolysis, and hypothalamic gene expression
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