Establishment of urinary exosome-like vesicles isolation protocol for FHHNC patients and evaluation of different exosomal RNA extraction methods View Full Text


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Article Info

DATE

2018-12

AUTHORS

M. Vall-Palomar, J. Arévalo, G. Ariceta, A. Meseguer

ABSTRACT

BACKGROUND: Molecular and cellular pathophysiological events occurring in the majority of rare kidney diseases remain to be elucidated. Familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC) is a rare autosomal recessive disorder caused by mutations in either CLDN16 or CLDN19 genes. This disease is characterized by massive urinary wasting of magnesium and calcium, osmosis deregulation and polyuria. Patients with p.G20D homozygous mutation in CLDN19 gene exhibit different progression to kidney failure suggesting that beyond the pathogenic mutation itself, other molecular events are favoring disease progression. Due to the fact that biopsy is not clinically indicated in these patients, urinary exosome-like vesicles (uEVs) can be envisioned as a valuable non-invasive source of information of events occurring in the kidney. Exosome research has increased notably to identify novel disease biomarkers but there is no consensus standardized protocols for uEVs isolation in patients with polyuria. For this reason, this work was aimed to evaluate and refine different uEVs isolation methods based on differential centrifugation, the gold standard method. RESULTS: Characterization by NTA, cryo-TEM and immunoblotting techniques identified the most appropriate protocol to obtain the highest yield and purest uEVs enriched fraction possible from urine control samples and FHHNC patients. Moreover, we tested five different RNA extraction methods and evaluated the miRNA expression pattern by qRT-PCR. CONCLUSIONS: In summary, we have standardized the conditions to proceed with the identification of differentially expressed miRNAs in uEVs of FHHNC patients, or other renal diseases characterized by polyuria. More... »

PAGES

278

References to SciGraph publications

  • 2006-04. Collection, storage, preservation, and normalization of human urinary exosomes for biomarker discovery in KIDNEY INTERNATIONAL
  • 2013-06. RenalTube: a network tool for clinical and genetic diagnosis of primary tubulopathies in EUROPEAN JOURNAL OF PEDIATRICS
  • 2014. Isolation of Urinary Exosomes for RNA Biomarker Discovery Using a Simple, Fast, and Highly Scalable Method in RNA MAPPING
  • 2008-12. Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers in NATURE CELL BIOLOGY
  • 2007-12. Exosomal transfer of proteins and RNAs at synapses in the nervous system in BIOLOGY DIRECT
  • 2015-08. Haplotype analysis of CLDN19 single nucleotide polymorphisms in Spanish patients with familial hypomagnesemia with hypercalciuria and nephrocalcinosis in WORLD JOURNAL OF PEDIATRICS
  • 2014-08. Characterization and deep sequencing analysis of exosomal and non-exosomal miRNA in human urine in KIDNEY INTERNATIONAL
  • 2010-04. Tamm-Horsfall protein and urinary exosome isolation in KIDNEY INTERNATIONAL
  • 2000-06. Familial hypomagnesaemia with hypercalciuria and nephrocalcinosis maps to chromosome 3q27 and is associated with mutations in the PCLN-1 gene in EUROPEAN JOURNAL OF HUMAN GENETICS
  • 2002-10. Proteomic analysis of normal human urinary proteins isolated by acetone precipitation or ultracentrifugation in KIDNEY INTERNATIONAL
  • 2012-11. Comparison of protein, microRNA, and mRNA yields using different methods of urinary exosome isolation for the discovery of kidney disease biomarkers in KIDNEY INTERNATIONAL
  • 2010-01. Astrocytes and Glioblastoma cells release exosomes carrying mtDNA in JOURNAL OF NEURAL TRANSMISSION
  • 2010-07. Nucleic acids within urinary exosomes/microvesicles are potential biomarkers for renal disease in KIDNEY INTERNATIONAL
  • 2010-03-13. Isolation and Purification of Exosomes in Urine in THE URINARY PROTEOME
  • 2007-06. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells in NATURE CELL BIOLOGY
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    http://scigraph.springernature.com/pub.10.1186/s12967-018-1651-z

    DOI

    http://dx.doi.org/10.1186/s12967-018-1651-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1107530007

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30305086


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