Expression of SOX2, NANOG and OCT4 in a mouse model of lipopolysaccharide-induced acute uterine injury and intrauterine adhesions View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Li Xiao, Yong Song, Wei Huang, Shiyuan Yang, Jing Fu, Xue Feng, Min Zhou

ABSTRACT

BACKGROUND: Activation of inflammation-mediated endometrial injury is suggested to play a decisive role in pathogenesis of intrauterine adhesion (IUA). The stem cell theory of endometrial diseases has been given a hotspot, in that human endometrial stem cells have been isolated from the endometrium. Three transcription factors that play key roles in maintaining pluripotency and self-renewal in stem cells are sex-determining region Y-box2 (SOX2), Nanog homebox (NANOG), and octamer-binding protein (OCT4), which may be responsible for the damage or repair process of uterine endometrium. We aim to investigate the expression of SOX2, NANOG and OCT4 in a mouse model of acute uterine injury induced by peritoneal injection of lipopolysaccharide (LPS) and also analyze their changes in endometrium of women with IUA. METHODS: The mouse uterine horns were collected at 0 h, 6 h, 12 h, 18 h or 24 h after a single dose of LPS or PBS injection. Meanwhile, we recruited 19 women with IUA diagnosed by hysteroscopy and 16 disease-free women as control group. Endometrial tissue samples were collected. SOX2, NANOG, and OCT4 expression were analyzed with Quantitative Real-time Polymerase Chain Reaction and Western blotting assay. RESULTS: In a mouse model of acute uterine injury, there was significant upregulation of NANOG at 6 h, SOX2 and OCT4 at 12 h compared with the values before injection or PBS injection. NANOG expression reached a peak at 6 h, while SOX2 and OCT4 peaked later at 12 h after LPS treatment. NANOG mRNA and protein expressions were significantly higher in endometrium of IUA patients compared to those of the control group. CONCLUSIONS: Expression of pluripotency factors SOX2, NANOG and OCT4 increased in a mouse model of LPS-induced acute uterine injury. NANOG peaked earlier followed by the other two factors before returning to baseline levels. NANOG but not SOX2 and OCT4 expression was overexpressed in the endometrium of women with IUA. They may be involved in the formation or restoration of IUA, and their roles in pathogenesis of IUA need to be further studied. More... »

PAGES

14

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12958-017-0234-9

DOI

http://dx.doi.org/10.1186/s12958-017-0234-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084251416

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28253866


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1114", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Paediatrics and Reproductive Medicine", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Acute Disease", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adult", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blotting, Western", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disease Models, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endometrium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hysteroscopy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lipopolysaccharides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred BALB C", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nanog Homeobox Protein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Octamer Transcription Factor-3", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pregnancy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reverse Transcriptase Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "SOXB1 Transcription Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Time Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tissue Adhesions", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Uterine Diseases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Young Adult", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Ministry of Education of the People's Republic of China", 
          "id": "https://www.grid.ac/institutes/grid.419897.a", 
          "name": [
            "Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, 610041, Chengdu, Sichuan, People\u2019s Republic of China", 
            "Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, 610041, Chengdu, Sichuan, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xiao", 
        "givenName": "Li", 
        "id": "sg:person.0753353136.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753353136.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ministry of Education of the People's Republic of China", 
          "id": "https://www.grid.ac/institutes/grid.419897.a", 
          "name": [
            "Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, 610041, Chengdu, Sichuan, People\u2019s Republic of China", 
            "Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, 610041, Chengdu, Sichuan, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Song", 
        "givenName": "Yong", 
        "id": "sg:person.01067601536.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067601536.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ministry of Education of the People's Republic of China", 
          "id": "https://www.grid.ac/institutes/grid.419897.a", 
          "name": [
            "Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, 610041, Chengdu, Sichuan, People\u2019s Republic of China", 
            "Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, 610041, Chengdu, Sichuan, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huang", 
        "givenName": "Wei", 
        "id": "sg:person.01135714736.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01135714736.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ministry of Education of the People's Republic of China", 
          "id": "https://www.grid.ac/institutes/grid.419897.a", 
          "name": [
            "Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, 610041, Chengdu, Sichuan, People\u2019s Republic of China", 
            "Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, 610041, Chengdu, Sichuan, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yang", 
        "givenName": "Shiyuan", 
        "id": "sg:person.0744403555.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744403555.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ministry of Education of the People's Republic of China", 
          "id": "https://www.grid.ac/institutes/grid.419897.a", 
          "name": [
            "Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, 610041, Chengdu, Sichuan, People\u2019s Republic of China", 
            "Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, 610041, Chengdu, Sichuan, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fu", 
        "givenName": "Jing", 
        "id": "sg:person.01076740130.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076740130.88"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ministry of Education of the People's Republic of China", 
          "id": "https://www.grid.ac/institutes/grid.419897.a", 
          "name": [
            "Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, 610041, Chengdu, Sichuan, People\u2019s Republic of China", 
            "Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, 610041, Chengdu, Sichuan, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Feng", 
        "givenName": "Xue", 
        "id": "sg:person.010227654615.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010227654615.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ministry of Education of the People's Republic of China", 
          "id": "https://www.grid.ac/institutes/grid.419897.a", 
          "name": [
            "Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, 610041, Chengdu, Sichuan, People\u2019s Republic of China", 
            "Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, 610041, Chengdu, Sichuan, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhou", 
        "givenName": "Min", 
        "id": "sg:person.0654363513.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654363513.85"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s0092-8674(03)00392-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004929245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.25.14.6031-6046.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010655768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1477-7827-12-42", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010766935", 
          "https://doi.org/10.1186/1477-7827-12-42"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/art.30326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011464947"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2008.04.043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011546580"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2008.01.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012430287"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0022550", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014338900"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1001/jama.292.1.81", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018000275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.fertnstert.2012.11.033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020843696"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.fertnstert.2005.02.056", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020950216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humupd/dml045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020987779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2249.2003.02358.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024098340"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2008.07.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024126337"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2013.01.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027238187"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0015-0282(16)59942-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034429761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0005959", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035799849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10397-008-0421-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037990402", 
          "https://doi.org/10.1007/s10397-008-0421-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1677/joe.0.0560133", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039035363"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod60.5.1158", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041437339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod60.5.1158", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041437339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1620/tjem.224.47", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043981543"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humrep/del514", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044132609"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0047-6374(96)01701-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045933289"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1151526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048905674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2005.08.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049638413"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2005.08.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049638413"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/wnr.0b013e328363fd67", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053528693"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/wnr.0b013e328363fd67", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053528693"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.2119/molmed.2009.00068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1068965207", 
          "https://doi.org/10.2119/molmed.2009.00068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.2119/molmed.2009.00068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1068965207", 
          "https://doi.org/10.2119/molmed.2009.00068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/mmr.2011.719", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071520358"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077325009", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078425653", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-12", 
    "datePublishedReg": "2017-12-01", 
    "description": "BACKGROUND: Activation of inflammation-mediated endometrial injury is suggested to play a decisive role in pathogenesis of intrauterine adhesion (IUA). The stem cell theory of endometrial diseases has been given a hotspot, in that human endometrial stem cells have been isolated from the endometrium. Three transcription factors that play key roles in maintaining pluripotency and self-renewal in stem cells are sex-determining region Y-box2 (SOX2), Nanog homebox (NANOG), and octamer-binding protein (OCT4), which may be responsible for the damage or repair process of uterine endometrium. We aim to investigate the expression of SOX2, NANOG and OCT4 in a mouse model of acute uterine injury induced by peritoneal injection of lipopolysaccharide (LPS) and also analyze their changes in endometrium of women with IUA.\nMETHODS: The mouse uterine horns were collected at 0\u00a0h, 6\u00a0h, 12\u00a0h, 18\u00a0h or 24\u00a0h after a single dose of LPS or PBS injection. Meanwhile, we recruited 19 women with IUA diagnosed by hysteroscopy and 16 disease-free women as control group. Endometrial tissue samples were collected. SOX2, NANOG, and OCT4 expression were analyzed with Quantitative Real-time Polymerase Chain Reaction and Western blotting assay.\nRESULTS: In a mouse model of acute uterine injury, there was significant upregulation of NANOG at 6\u00a0h, SOX2 and OCT4 at 12\u00a0h compared with the values before injection or PBS injection. NANOG expression reached a peak at 6\u00a0h, while SOX2 and OCT4 peaked later at 12\u00a0h after LPS treatment. NANOG mRNA and protein expressions were significantly higher in endometrium of IUA patients compared to those of the control group.\nCONCLUSIONS: Expression of pluripotency factors SOX2, NANOG and OCT4 increased in a mouse model of LPS-induced acute uterine injury. NANOG peaked earlier followed by the other two factors before returning to baseline levels. NANOG but not SOX2 and OCT4 expression was overexpressed in the endometrium of women with IUA. They may be involved in the formation or restoration of IUA, and their roles in pathogenesis of IUA need to be further studied.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s12958-017-0234-9", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1031327", 
        "issn": [
          "1477-7827"
        ], 
        "name": "Reproductive Biology and Endocrinology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "name": "Expression of SOX2, NANOG and OCT4 in a mouse model of lipopolysaccharide-induced acute uterine injury and intrauterine adhesions", 
    "pagination": "14", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "53821c9e2c2eaa4d3a839b49e3c01cc793baba1161290820bc03cb5887997aa2"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28253866"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101153627"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s12958-017-0234-9"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1084251416"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s12958-017-0234-9", 
      "https://app.dimensions.ai/details/publication/pub.1084251416"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:39", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000346_0000000346/records_99836_00000004.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs12958-017-0234-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12958-017-0234-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12958-017-0234-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12958-017-0234-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12958-017-0234-9'


 

This table displays all metadata directly associated to this object as RDF triples.

283 TRIPLES      21 PREDICATES      79 URIs      42 LITERALS      30 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s12958-017-0234-9 schema:about N00db779d0e2d4e9da2f5ff103b3b1efd
2 N01e9b940dc3846c780f5eb32f821db18
3 N0cc407832fd14d94b585a8528e9e8ccb
4 N11460edeb7314644bf9d560e7dd6e82d
5 N1c21e3fd81534896bfadaa7fe4960b71
6 N2749b7fea8b949d39a4bd2f8855bc92f
7 N2d3464ba668e415f8fbf9b8261f6105d
8 N5a9df616d478488483105572b7b4af89
9 N68e1aedd457f425e868bed733ed7528b
10 N76b82a5ea8cd41249fafeefd5f970e70
11 N8fa57a839bf84817989d9d418accdec6
12 Nb174003dd7e840e9b89985efef6018c5
13 Nb48e87debcf5471399b63a9484043801
14 Nb99c5020cb794c53a7358523704b39ed
15 Nbd51f60298b34a5081504750a6e58514
16 Nc8be52d925834555a3f17fc33e3d5369
17 Nd8636eb12f894244a24db31b5f48adb9
18 Ndeaa2d2187e14cdfacb2dc67b0f325c4
19 Ne25553be188343c8a1c4a02c3d8a24fa
20 Nf466d52789fd4e0dac3bdea00191d206
21 Nf8d535d9945e45d691ae353fbc15a596
22 anzsrc-for:11
23 anzsrc-for:1114
24 schema:author Ndde73b36c1e5409d91d8fbb09c784947
25 schema:citation sg:pub.10.1007/s10397-008-0421-y
26 sg:pub.10.1186/1477-7827-12-42
27 sg:pub.10.2119/molmed.2009.00068
28 https://app.dimensions.ai/details/publication/pub.1077325009
29 https://app.dimensions.ai/details/publication/pub.1078425653
30 https://doi.org/10.1001/jama.292.1.81
31 https://doi.org/10.1002/art.30326
32 https://doi.org/10.1016/0047-6374(96)01701-0
33 https://doi.org/10.1016/j.cell.2005.08.020
34 https://doi.org/10.1016/j.cell.2008.01.015
35 https://doi.org/10.1016/j.cell.2008.04.043
36 https://doi.org/10.1016/j.fertnstert.2005.02.056
37 https://doi.org/10.1016/j.fertnstert.2012.11.033
38 https://doi.org/10.1016/j.stem.2008.07.001
39 https://doi.org/10.1016/j.stem.2013.01.007
40 https://doi.org/10.1016/s0015-0282(16)59942-7
41 https://doi.org/10.1016/s0092-8674(03)00392-1
42 https://doi.org/10.1093/humrep/del514
43 https://doi.org/10.1093/humupd/dml045
44 https://doi.org/10.1095/biolreprod60.5.1158
45 https://doi.org/10.1097/wnr.0b013e328363fd67
46 https://doi.org/10.1111/j.1365-2249.2003.02358.x
47 https://doi.org/10.1126/science.1151526
48 https://doi.org/10.1128/mcb.25.14.6031-6046.2005
49 https://doi.org/10.1371/journal.pone.0005959
50 https://doi.org/10.1371/journal.pone.0022550
51 https://doi.org/10.1620/tjem.224.47
52 https://doi.org/10.1677/joe.0.0560133
53 https://doi.org/10.3892/mmr.2011.719
54 schema:datePublished 2017-12
55 schema:datePublishedReg 2017-12-01
56 schema:description BACKGROUND: Activation of inflammation-mediated endometrial injury is suggested to play a decisive role in pathogenesis of intrauterine adhesion (IUA). The stem cell theory of endometrial diseases has been given a hotspot, in that human endometrial stem cells have been isolated from the endometrium. Three transcription factors that play key roles in maintaining pluripotency and self-renewal in stem cells are sex-determining region Y-box2 (SOX2), Nanog homebox (NANOG), and octamer-binding protein (OCT4), which may be responsible for the damage or repair process of uterine endometrium. We aim to investigate the expression of SOX2, NANOG and OCT4 in a mouse model of acute uterine injury induced by peritoneal injection of lipopolysaccharide (LPS) and also analyze their changes in endometrium of women with IUA. METHODS: The mouse uterine horns were collected at 0 h, 6 h, 12 h, 18 h or 24 h after a single dose of LPS or PBS injection. Meanwhile, we recruited 19 women with IUA diagnosed by hysteroscopy and 16 disease-free women as control group. Endometrial tissue samples were collected. SOX2, NANOG, and OCT4 expression were analyzed with Quantitative Real-time Polymerase Chain Reaction and Western blotting assay. RESULTS: In a mouse model of acute uterine injury, there was significant upregulation of NANOG at 6 h, SOX2 and OCT4 at 12 h compared with the values before injection or PBS injection. NANOG expression reached a peak at 6 h, while SOX2 and OCT4 peaked later at 12 h after LPS treatment. NANOG mRNA and protein expressions were significantly higher in endometrium of IUA patients compared to those of the control group. CONCLUSIONS: Expression of pluripotency factors SOX2, NANOG and OCT4 increased in a mouse model of LPS-induced acute uterine injury. NANOG peaked earlier followed by the other two factors before returning to baseline levels. NANOG but not SOX2 and OCT4 expression was overexpressed in the endometrium of women with IUA. They may be involved in the formation or restoration of IUA, and their roles in pathogenesis of IUA need to be further studied.
57 schema:genre research_article
58 schema:inLanguage en
59 schema:isAccessibleForFree true
60 schema:isPartOf N22ac05a034654ed9be8213626aaeb824
61 N6e6bf70a35384b9d8b1a34636227e4f1
62 sg:journal.1031327
63 schema:name Expression of SOX2, NANOG and OCT4 in a mouse model of lipopolysaccharide-induced acute uterine injury and intrauterine adhesions
64 schema:pagination 14
65 schema:productId N0b792b17fd9842e38d302040167736d5
66 N2f2ff92a0b7c4f42a9f15c09f05f7464
67 N388d6183d953462abba6a6edc1b738af
68 Nb7a0b79e97274a51b4a069ca750b27d2
69 Nc8d10abeef7a49bb9ec3d5db34a3d695
70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084251416
71 https://doi.org/10.1186/s12958-017-0234-9
72 schema:sdDatePublished 2019-04-11T09:39
73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
74 schema:sdPublisher N6bd52c6c7b0f43a6b718b9bebd1a2a81
75 schema:url https://link.springer.com/10.1186%2Fs12958-017-0234-9
76 sgo:license sg:explorer/license/
77 sgo:sdDataset articles
78 rdf:type schema:ScholarlyArticle
79 N00db779d0e2d4e9da2f5ff103b3b1efd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Reverse Transcriptase Polymerase Chain Reaction
81 rdf:type schema:DefinedTerm
82 N01e9b940dc3846c780f5eb32f821db18 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Lipopolysaccharides
84 rdf:type schema:DefinedTerm
85 N0a301d354b8743008ad441cfddfe0648 rdf:first sg:person.0654363513.85
86 rdf:rest rdf:nil
87 N0b792b17fd9842e38d302040167736d5 schema:name doi
88 schema:value 10.1186/s12958-017-0234-9
89 rdf:type schema:PropertyValue
90 N0cc407832fd14d94b585a8528e9e8ccb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Acute Disease
92 rdf:type schema:DefinedTerm
93 N11460edeb7314644bf9d560e7dd6e82d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Endometrium
95 rdf:type schema:DefinedTerm
96 N1c21e3fd81534896bfadaa7fe4960b71 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Gene Expression Regulation
98 rdf:type schema:DefinedTerm
99 N22ac05a034654ed9be8213626aaeb824 schema:issueNumber 1
100 rdf:type schema:PublicationIssue
101 N2749b7fea8b949d39a4bd2f8855bc92f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Blotting, Western
103 rdf:type schema:DefinedTerm
104 N2d3464ba668e415f8fbf9b8261f6105d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Animals
106 rdf:type schema:DefinedTerm
107 N2f2ff92a0b7c4f42a9f15c09f05f7464 schema:name pubmed_id
108 schema:value 28253866
109 rdf:type schema:PropertyValue
110 N388d6183d953462abba6a6edc1b738af schema:name readcube_id
111 schema:value 53821c9e2c2eaa4d3a839b49e3c01cc793baba1161290820bc03cb5887997aa2
112 rdf:type schema:PropertyValue
113 N3c45cef899254aeca2a454be4367f0dd rdf:first sg:person.01067601536.61
114 rdf:rest Ne407b9ecf91f4afaa4951d2fa87ad57b
115 N5a0055b56a5741839b4a8d8f8fb148ac rdf:first sg:person.0744403555.01
116 rdf:rest N7b2aca1422f441aa87cac3ef000632d8
117 N5a9df616d478488483105572b7b4af89 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Young Adult
119 rdf:type schema:DefinedTerm
120 N5bb316a95808481c9a4b578af553314b rdf:first sg:person.010227654615.01
121 rdf:rest N0a301d354b8743008ad441cfddfe0648
122 N68e1aedd457f425e868bed733ed7528b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Female
124 rdf:type schema:DefinedTerm
125 N6bd52c6c7b0f43a6b718b9bebd1a2a81 schema:name Springer Nature - SN SciGraph project
126 rdf:type schema:Organization
127 N6e6bf70a35384b9d8b1a34636227e4f1 schema:volumeNumber 15
128 rdf:type schema:PublicationVolume
129 N76b82a5ea8cd41249fafeefd5f970e70 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Hysteroscopy
131 rdf:type schema:DefinedTerm
132 N7b2aca1422f441aa87cac3ef000632d8 rdf:first sg:person.01076740130.88
133 rdf:rest N5bb316a95808481c9a4b578af553314b
134 N8fa57a839bf84817989d9d418accdec6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Pregnancy
136 rdf:type schema:DefinedTerm
137 Nb174003dd7e840e9b89985efef6018c5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Mice, Inbred BALB C
139 rdf:type schema:DefinedTerm
140 Nb48e87debcf5471399b63a9484043801 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Disease Models, Animal
142 rdf:type schema:DefinedTerm
143 Nb7a0b79e97274a51b4a069ca750b27d2 schema:name dimensions_id
144 schema:value pub.1084251416
145 rdf:type schema:PropertyValue
146 Nb99c5020cb794c53a7358523704b39ed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Nanog Homeobox Protein
148 rdf:type schema:DefinedTerm
149 Nbd51f60298b34a5081504750a6e58514 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Tissue Adhesions
151 rdf:type schema:DefinedTerm
152 Nc8be52d925834555a3f17fc33e3d5369 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Uterine Diseases
154 rdf:type schema:DefinedTerm
155 Nc8d10abeef7a49bb9ec3d5db34a3d695 schema:name nlm_unique_id
156 schema:value 101153627
157 rdf:type schema:PropertyValue
158 Nd8636eb12f894244a24db31b5f48adb9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Time Factors
160 rdf:type schema:DefinedTerm
161 Ndde73b36c1e5409d91d8fbb09c784947 rdf:first sg:person.0753353136.48
162 rdf:rest N3c45cef899254aeca2a454be4367f0dd
163 Ndeaa2d2187e14cdfacb2dc67b0f325c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Adult
165 rdf:type schema:DefinedTerm
166 Ne25553be188343c8a1c4a02c3d8a24fa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name SOXB1 Transcription Factors
168 rdf:type schema:DefinedTerm
169 Ne407b9ecf91f4afaa4951d2fa87ad57b rdf:first sg:person.01135714736.25
170 rdf:rest N5a0055b56a5741839b4a8d8f8fb148ac
171 Nf466d52789fd4e0dac3bdea00191d206 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Humans
173 rdf:type schema:DefinedTerm
174 Nf8d535d9945e45d691ae353fbc15a596 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
175 schema:name Octamer Transcription Factor-3
176 rdf:type schema:DefinedTerm
177 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
178 schema:name Medical and Health Sciences
179 rdf:type schema:DefinedTerm
180 anzsrc-for:1114 schema:inDefinedTermSet anzsrc-for:
181 schema:name Paediatrics and Reproductive Medicine
182 rdf:type schema:DefinedTerm
183 sg:journal.1031327 schema:issn 1477-7827
184 schema:name Reproductive Biology and Endocrinology
185 rdf:type schema:Periodical
186 sg:person.010227654615.01 schema:affiliation https://www.grid.ac/institutes/grid.419897.a
187 schema:familyName Feng
188 schema:givenName Xue
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010227654615.01
190 rdf:type schema:Person
191 sg:person.01067601536.61 schema:affiliation https://www.grid.ac/institutes/grid.419897.a
192 schema:familyName Song
193 schema:givenName Yong
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067601536.61
195 rdf:type schema:Person
196 sg:person.01076740130.88 schema:affiliation https://www.grid.ac/institutes/grid.419897.a
197 schema:familyName Fu
198 schema:givenName Jing
199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076740130.88
200 rdf:type schema:Person
201 sg:person.01135714736.25 schema:affiliation https://www.grid.ac/institutes/grid.419897.a
202 schema:familyName Huang
203 schema:givenName Wei
204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01135714736.25
205 rdf:type schema:Person
206 sg:person.0654363513.85 schema:affiliation https://www.grid.ac/institutes/grid.419897.a
207 schema:familyName Zhou
208 schema:givenName Min
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654363513.85
210 rdf:type schema:Person
211 sg:person.0744403555.01 schema:affiliation https://www.grid.ac/institutes/grid.419897.a
212 schema:familyName Yang
213 schema:givenName Shiyuan
214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744403555.01
215 rdf:type schema:Person
216 sg:person.0753353136.48 schema:affiliation https://www.grid.ac/institutes/grid.419897.a
217 schema:familyName Xiao
218 schema:givenName Li
219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753353136.48
220 rdf:type schema:Person
221 sg:pub.10.1007/s10397-008-0421-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1037990402
222 https://doi.org/10.1007/s10397-008-0421-y
223 rdf:type schema:CreativeWork
224 sg:pub.10.1186/1477-7827-12-42 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010766935
225 https://doi.org/10.1186/1477-7827-12-42
226 rdf:type schema:CreativeWork
227 sg:pub.10.2119/molmed.2009.00068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068965207
228 https://doi.org/10.2119/molmed.2009.00068
229 rdf:type schema:CreativeWork
230 https://app.dimensions.ai/details/publication/pub.1077325009 schema:CreativeWork
231 https://app.dimensions.ai/details/publication/pub.1078425653 schema:CreativeWork
232 https://doi.org/10.1001/jama.292.1.81 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018000275
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1002/art.30326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011464947
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/0047-6374(96)01701-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045933289
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/j.cell.2005.08.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049638413
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/j.cell.2008.01.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012430287
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/j.cell.2008.04.043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011546580
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/j.fertnstert.2005.02.056 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020950216
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/j.fertnstert.2012.11.033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020843696
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/j.stem.2008.07.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024126337
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/j.stem.2013.01.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027238187
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/s0015-0282(16)59942-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034429761
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/s0092-8674(03)00392-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004929245
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1093/humrep/del514 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044132609
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1093/humupd/dml045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020987779
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1095/biolreprod60.5.1158 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041437339
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1097/wnr.0b013e328363fd67 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053528693
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1111/j.1365-2249.2003.02358.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024098340
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1126/science.1151526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048905674
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1128/mcb.25.14.6031-6046.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010655768
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1371/journal.pone.0005959 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035799849
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1371/journal.pone.0022550 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014338900
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1620/tjem.224.47 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043981543
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1677/joe.0.0560133 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039035363
277 rdf:type schema:CreativeWork
278 https://doi.org/10.3892/mmr.2011.719 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071520358
279 rdf:type schema:CreativeWork
280 https://www.grid.ac/institutes/grid.419897.a schema:alternateName Ministry of Education of the People's Republic of China
281 schema:name Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, 610041, Chengdu, Sichuan, People’s Republic of China
282 Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, 610041, Chengdu, Sichuan, People’s Republic of China
283 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...