Hemin enhances the cardioprotective effects of mesenchymal stem cell-derived exosomes against infarction via amelioration of cardiomyocyte senescence View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2021-10-21

AUTHORS

Huifeng Zheng, Xiaoting Liang, Qian Han, Zhuang Shao, Yuxiao Zhang, Linli Shi, Yimei Hong, Weifeng Li, Cong Mai, Qingwen Mo, Qingling Fu, Xiaoxue Ma, Fang Lin, Mimi Li, Bei Hu, Xin Li, Yuelin Zhang

ABSTRACT

BACKGROUND: Application of mesenchymal stem cell-derived exosomes (MSC-EXO) has emerged as a novel therapeutic strategy for myocardial infarction (MI). Our previous study showed that pretreatment with hemin, a potent heme oxygenase-1 (HO-1) inducer, enhanced the cardioprotective effects of MSCs in a mouse model of MI. This study aimed to investigate the therapeutic effects of EXO derived from hemin-pretreated MSCs (Hemin-MSC-EXO) in MI and explore the potential mechanisms. METHODS: MSC-EXO and Hemin-MSC-EXO were collected and characterized. MSC-EXO and Hemin-MSC-EXO were intramuscularly injected into the peri-infarct region in a mouse model of MI. Heart function of mice was assessed by echocardiography. The mitochondrial morphology of neonatal mice cardiomyocytes (NMCMs) under serum deprivation and hypoxic (SD/H) conditions was examined by Mitotracker staining. The cellular senescence of NMCMs was determined by senescence-associated-β-galactosidase assay. A loss-of-function approach was adopted to determine the role of Hemin-MSC-exosomal-miR-183-5p in the regulation of cardiomyocyte senescence RESULTS: EXO were successfully isolated from the supernatant of MSCs and Hemin-pretreated MSCs. Compared with MSC-EXO, injection of Hemin-MSC-EXO significantly improved cardiac function and reduced fibrosis. Both MSC-EXO and Hemin-MSC-EXO ameliorated cardiomyocyte senescence and mitochondrial fission in vitro and in vivo, and the latter exhibited better protective effects. MicroRNA sequencing revealed a higher level of miR-183-5p in Hemin-MSC-EXO than in MSC-EXO. MiR-183-5p knockdown partially abrogated the protective effects of Hemin-MSC-EXO in attenuating mitochondrial fission and cellular senescence of cardiomyocytes induced by SD/H. High mobility group box-1 (HMGB1) abundance was lower in Hemin-MSC-EXO-treated than MSC-EXO-treated mouse hearts, and HMGB1 was identified as one of the potential target genes of miR-183-5p. Mechanistically, Hemin-MSC-EXO inhibited SD/H-induced cardiomyocyte senescence partially by delivering miR-183-5p into recipient cardiomyocytes via regulation of the HMGB1/ERK pathway. Furthermore, knockdown of miR-183-5p reduced the Hemin-MSC-EXO-mediated cardioprotective effects in a mouse model of MI. CONCLUSION: Our results reveal that Hemin-MSC-EXO are superior to MSC-EXO in treating MI. Exosomal miR-183-5p mediates, at least partially, the cardioprotective effects of Hemin-MSC-EXO by inhibiting cardiomyocyte senescence via regulation of the HMGB1/ERK pathway. This study highlights that MSC-EXO have high translational value in repairing cardiac dysfunction following infarction. More... »

PAGES

332

References to SciGraph publications

  • 2019-12-17. LncRNA KLF3-AS1 in human mesenchymal stem cell-derived exosomes ameliorates pyroptosis of cardiomyocytes and myocardial infarction through miR-138-5p/Sirt1 axis in STEM CELL RESEARCH & THERAPY
  • 2015-07-17. E2F1-dependent miR-421 regulates mitochondrial fragmentation and myocardial infarction by targeting Pink1 in NATURE COMMUNICATIONS
  • 2021-02-27. Macrophage migration inhibitory factor facilitates the therapeutic efficacy of mesenchymal stem cells derived exosomes in acute myocardial infarction through upregulating miR-133a-3p in JOURNAL OF NANOBIOTECHNOLOGY
  • 2020-07-03. Myocardial ischaemia–reperfusion injury and cardioprotection in perspective in NATURE REVIEWS CARDIOLOGY
  • 2020-05-05. Exosomal miR-25-3p from mesenchymal stem cells alleviates myocardial infarction by targeting pro-apoptotic proteins and EZH2 in CELL DEATH & DISEASE
  • 2018-09-26. HMGB1 promotes ERK-mediated mitochondrial Drp1 phosphorylation for chemoresistance through RAGE in colorectal cancer in CELL DEATH & DISEASE
  • 2021-05-21. Exosomes derived from pioglitazone-pretreated MSCs accelerate diabetic wound healing through enhancing angiogenesis in JOURNAL OF NANOBIOTECHNOLOGY
  • 2021-02-26. Inhibition of HMGB1 reduced high glucose-induced BMSCs apoptosis via activation of AMPK and regulation of mitochondrial functions in JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
  • 2019-05-10. Cardioprotective effects of microRNA-18a on acute myocardial infarction by promoting cardiomyocyte autophagy and suppressing cellular senescence via brain derived neurotrophic factor in CELL & BIOSCIENCE
  • 2015-05-21. Activation of NRG1-ERBB4 signaling potentiates mesenchymal stem cell-mediated myocardial repairs following myocardial infarction in CELL DEATH & DISEASE
  • 2019-11-04. Quantitative proteomic analyses reveal that GPX4 downregulation during myocardial infarction contributes to ferroptosis in cardiomyocytes in CELL DEATH & DISEASE
  • 2020-05-21. Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamics in NATURE COMMUNICATIONS
  • 2020-10-25. GDF11 inhibits cardiomyocyte pyroptosis and exerts cardioprotection in acute myocardial infarction mice by upregulation of transcription factor HOXA3 in CELL DEATH & DISEASE
  • 2019-03-07. Potent immunomodulation and angiogenic effects of mesenchymal stem cells versus cardiomyocytes derived from pluripotent stem cells for treatment of heart failure in STEM CELL RESEARCH & THERAPY
  • 2020-08-28. HIF-1α overexpression in mesenchymal stem cell-derived exosomes mediates cardioprotection in myocardial infarction by enhanced angiogenesis in STEM CELL RESEARCH & THERAPY
  • 2020-06-08. Hypoxia-challenged MSC-derived exosomes deliver miR-210 to attenuate post-infarction cardiac apoptosis in STEM CELL RESEARCH & THERAPY
  • 2017-07-25. A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure in NATURE COMMUNICATIONS
  • 2018-06-05. Therapeutic approaches for cardiac regeneration and repair in NATURE REVIEWS CARDIOLOGY
  • 2015-07-10. Intracoronary infusion of Wharton’s jelly-derived mesenchymal stem cells in acute myocardial infarction: double-blind, randomized controlled trial in BMC MEDICINE
  • 2021-07-07. Hypoxia adipose stem cell-derived exosomes promote high-quality healing of diabetic wound involves activation of PI3K/Akt pathways in JOURNAL OF NANOBIOTECHNOLOGY
  • 2020-08-27. Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages in NATURE COMMUNICATIONS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12951-021-01077-y

    DOI

    http://dx.doi.org/10.1186/s12951-021-01077-y

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1142047962

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/34674708


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