Development of gold nanoparticles biosensor for ultrasensitive diagnosis of foot and mouth disease virus View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-12

AUTHORS

Mervat E. Hamdy, Michele Del Carlo, Hussein A. Hussein, Taher A. Salah, Ayman H. El-Deeb, Mohamed M. Emara, Guilia Pezzoni, Dario Compagnone

ABSTRACT

BACKGROUND: Nano-PCR is a recent tool that is used in viral diseases diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the diagnosis of different diseases including viral diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV. RESULTS: The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivity of the AuNPs-FMDV biosensor was 10 copy number RNA standards in RT-PCR and 1 copy number RNA standard in real-time rRT-PCR with a 94.5% efficiency, 0.989 R2, a - 3.544 slope and 100% specificity (no cross-reactivity with SVDV). These findings were confirmed by the specific and sensitive recognition of 31 Egyptian FMDV clinical isolates that represents the three FMDV serotypes (O, A, and SAT2). CONCLUSIONS: The AuNPs-FMDV biosensor presents in this study demonstrates a superior analytical and clinical performance for FMDV diagnosis. In addition, this biosensor has a simple workflow and accelerates epidemiological surveillance, hence, it is qualified as an efficient FMDV diagnosis tool for quarantine stations and farms particularly in FMDV endemic areas. More... »

PAGES

48

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12951-018-0374-x

DOI

http://dx.doi.org/10.1186/s12951-018-0374-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1103951159

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29751767


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