Plasma-derived exosomal miR-15a-5p as a promising diagnostic biomarker for early detection of endometrial carcinoma View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2021-03-29

AUTHORS

Lanyun Zhou, Wei Wang, Fenfen Wang, Siqi Yang, Jiaqi Hu, Bingjian Lu, Zimin Pan, Yu Ma, Mengyue Zheng, Liyuan Zhou, Shufeng Lei, Penghong Song, Pengyuan Liu, Weiguo Lu, Yan Lu

ABSTRACT

Endometrial cancer (EC) is a major cause of death among gynecologic malignancies. To improve early detection of EC in patients, we carried out a large plasma-derived exosomal microRNA (miRNA) studies for diagnostic biomarker discovery in EC. Small RNA sequencing was performed to identify candidate exosomal miRNAs as diagnostic biomarkers in 56 plasma samples from healthy subjects and EC patients. These miRNA candidates were further validated in 202 independent plasma samples by droplet digital PCR (ddPCR), 32 pairs of endometrial tumors and adjacent normal tissues by quantitative real-time PCR (qRT-PCR), and matched plasma samples of 12 patients before and after surgery by ddPCR. miR-15a-5p, miR-106b-5p, and miR107 were significantly upregulated in exomes isolated from plasma samples of EC patients compared with healthy subjects. Particularly, miR-15a-5p alone yielded an AUC value of 0.813 to distinguish EC patients with stage I from healthy subjects. The integration of miR-15a-5p and serum tumor markers (CEA and CA125) achieved a higher AUC value of 0.899. There was also a close connection between miR-15a-5p and clinical manifestations in EC patients. Its exosomal expression was not only associated with the depth of muscular infiltration and aggressiveness of EC, but also correlated with levels of reproductive hormones such as TTE and DHEAS. Collectively, plasma-derived exosomal miR-15a-5p is a promising and effective diagnostic biomarker for the early detection of endometrial cancer. More... »

PAGES

57

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12943-021-01352-4

DOI

http://dx.doi.org/10.1186/s12943-021-01352-4

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https://app.dimensions.ai/details/publication/pub.1136755712

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/33781255


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20 schema:description Endometrial cancer (EC) is a major cause of death among gynecologic malignancies. To improve early detection of EC in patients, we carried out a large plasma-derived exosomal microRNA (miRNA) studies for diagnostic biomarker discovery in EC. Small RNA sequencing was performed to identify candidate exosomal miRNAs as diagnostic biomarkers in 56 plasma samples from healthy subjects and EC patients. These miRNA candidates were further validated in 202 independent plasma samples by droplet digital PCR (ddPCR), 32 pairs of endometrial tumors and adjacent normal tissues by quantitative real-time PCR (qRT-PCR), and matched plasma samples of 12 patients before and after surgery by ddPCR. miR-15a-5p, miR-106b-5p, and miR107 were significantly upregulated in exomes isolated from plasma samples of EC patients compared with healthy subjects. Particularly, miR-15a-5p alone yielded an AUC value of 0.813 to distinguish EC patients with stage I from healthy subjects. The integration of miR-15a-5p and serum tumor markers (CEA and CA125) achieved a higher AUC value of 0.899. There was also a close connection between miR-15a-5p and clinical manifestations in EC patients. Its exosomal expression was not only associated with the depth of muscular infiltration and aggressiveness of EC, but also correlated with levels of reproductive hormones such as TTE and DHEAS. Collectively, plasma-derived exosomal miR-15a-5p is a promising and effective diagnostic biomarker for the early detection of endometrial cancer.
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27 DHEAS
28 EC patients
29 PCR
30 RNA sequencing
31 TTE
32 adjacent normal tissues
33 aggressiveness
34 biomarker discovery
35 biomarkers
36 cancer
37 candidate exosomal miRNAs
38 candidates
39 carcinoma
40 cause
41 clinical manifestations
42 close connection
43 connection
44 death
45 depth
46 detection
47 diagnostic biomarker discovery
48 diagnostic biomarkers
49 digital PCR
50 discovery
51 droplet digital PCR
52 early detection
53 effective diagnostic biomarkers
54 endometrial cancer
55 endometrial carcinoma
56 endometrial tumors
57 exome
58 exosomal expression
59 exosomal miRNAs
60 expression
61 gynecologic malignancies
62 healthy subjects
63 highest AUC value
64 hormone
65 infiltration
66 integration
67 levels
68 major cause
69 malignancy
70 manifestations
71 markers
72 miR
73 miR107
74 miRNA candidates
75 miRNAs
76 muscular infiltration
77 normal tissues
78 pairs
79 patients
80 plasma samples
81 promising diagnostic biomarker
82 quantitative real-time PCR
83 real-time PCR
84 reproductive hormones
85 samples
86 sequencing
87 serum tumor markers
88 small RNA sequencing
89 stage I
90 study
91 subjects
92 surgery
93 tissue
94 tumor markers
95 tumors
96 values
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