Evaluation the reactivity of a peptide-based monoclonal antibody derived from OmpA with drug resistant pulsotypes of Acinetobacter baumannii as a ... View Full Text


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Article Info

DATE

2022-06-30

AUTHORS

Omid Yeganeh, Mahdi Shabani, Parviz Pakzad, Nariman Mosaffa, Ali Hashemi

ABSTRACT

BackgroundAcinetobacter baumannii is an opportunistic and antibiotic-resistant pathogen that predominantly causes nosocomial infections. There is urgent need for development nonantibiotic-based treatment strategies. We developed a novel monoclonal antibody (mAb) against a peptide of conserved outer membrane protein A (OmpA) and evaluated its reactivity with different pulsotypes of A. baumannii.MethodsPeptide derived from A. baumannii OmpA was conjugated to keyhole limpet hemocyanin and injected into BALB/c mice. Splenocytes of immunized mice were fused with SP2/0 myeloma cells followed by selection of antibody-producing hybridoma cells. After screening of different hybridoma colonies by ELISA, one monoclone was selected as 3F10-C9 and the antibody was tested for reaction with five different Acinetobacter pulsotypes that were resistant to carbapenem antibiotics. The affinity constant was measured by ELISA. The ELISA, western blotting, indirect immunofluorescence (IFA), and in vitro opsonophagocytosis assays were used to evaluate the reactivity of generated mAb.ResultsThe anti-OmpA antibody reacted with the immunizing peptide and had a high affinity (1.94 × 10−9 M) for its antigen in the ELISA. Specific binding of mAb to OmpA was confirmed in Western blot. IFA assays revealed that mAb recognized specific OmpA on the pulsotypes. Opsonophagocytosis assays showed that the mAb increased the bactericidal activity of macrophage cells. The antibody function was higher in the presence of serum complement.ConclusionsThe peptide-based mAb demonstrated optimal performance in laboratory experiments which may be appropriate in investigation on OmpA in Acinetobacter pathogenesis and development of passive immunization as a novel therapeutic approach. More... »

PAGES

30

References to SciGraph publications

  • 2017-12-27. Antigenic Properties of Iron Regulated Proteins in Acinetobacter baumannii: An In Silico Approach in INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
  • 2019-07-24. DNA vaccine encoding OmpA and Pal from Acinetobacter baumannii efficiently protects mice against pulmonary infection in MOLECULAR BIOLOGY REPORTS
  • 2021-10-26. Prevalence of β-lactamase-encoding genes and molecular typing of Acinetobacter baumannii isolates carrying carbapenemase OXA-24 in children in ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
  • 2016-12-05. Ultrastructural Characterization of the Lower Motor System in a Mouse Model of Krabbe Disease in SCIENTIFIC REPORTS
  • 2017-12-29. Comparison of efficacies of fetal bovine sera from different suppliers in cell culture experiments in COMPARATIVE CLINICAL PATHOLOGY
  • 2021-06-27. Multidrug-resistant Acinetobacter baumannii infections in COVID-19 patients hospitalized in intensive care unit in INFECTION
  • 2001-08-09. Monoclonal antibodies against the iron regulated outer membrane Proteins of Acinetobacter baumannii are bactericidal in BMC MICROBIOLOGY
  • 2021-01-12. Outer membrane vesicles containing OmpA induce mitochondrial fragmentation to promote pathogenesis of Acinetobacter baumannii in SCIENTIFIC REPORTS
  • 2021-08-27. Screening of small molecules attenuating biofilm formation of Acinetobacter baumannii by inhibition of ompA promoter activity in JOURNAL OF MICROBIOLOGY
  • 2020-01-30. Multi-drug resistance of blood stream, urinary tract and surgical site nosocomial infections of Acinetobacter baumannii and Pseudomonas aeruginosa among patients hospitalized at Felegehiwot referral hospital, Northwest Ethiopia: a cross-sectional study in BMC INFECTIOUS DISEASES
  • 2015. Peptide-Carrier Conjugation in PEPTIDE ANTIBODIES
  • 2021-04-27. A conserved region of Acinetobacter trimeric autotransporter adhesion, Ata, provokes suppression of Acinetobacter baumannii virulence in ARCHIVES OF MICROBIOLOGY
  • 2022-01-07. The characteristic and potential therapeutic effect of isolated multidrug-resistant Acinetobacter baumannii lytic phage in ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
  • 2020-01-18. Outer membrane protein A (OmpA) as a potential therapeutic target for Acinetobacter baumannii infection in JOURNAL OF BIOMEDICAL SCIENCE
  • 2016-02-08. Immunization with a 22-kDa outer membrane protein elicits protective immunity to multidrug-resistant Acinetobacter baumannii in SCIENTIFIC REPORTS
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    http://scigraph.springernature.com/pub.10.1186/s12941-022-00523-5

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    81 infection
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    91 monoclones
    92 myeloma cells
    93 need
    94 nosocomial infections
    95 novel monoclonal antibody
    96 novel therapeutic approaches
    97 opsonophagocytosis
    98 opsonophagocytosis assay
    99 optimal performance
    100 outer membrane protein A
    101 passive immunization
    102 pathogenesis
    103 pathogens
    104 peptides
    105 performance
    106 potential therapeutic approach
    107 presence
    108 protein A
    109 pulsotypes
    110 reaction
    111 reactivity
    112 selection
    113 serum complement
    114 specific binding
    115 splenocytes
    116 strategies
    117 therapeutic approaches
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