Molecular characterization of β-lactamase genes in clinical isolates of carbapenem-resistant Acinetobacter baumannii View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-11-16

AUTHORS

Kevin M. Raible, Bhaswati Sen, Nancy Law, Tiffany E. Bias, Christopher L. Emery, Garth D. Ehrlich, Suresh G. Joshi

ABSTRACT

BackgroundAcinetobacter baumannii is a nosocomial pathogen which is establishing as a major cause of morbidity and mortality within the healthcare community. The success of this pathogen is largely due to its ability to rapidly gain resistance to antimicrobial therapies and its capability to persist in an abiotic environment through the production of a biofilm. Our tertiary-care hospital has showed high incidence of carbapenem-resistant Acinetobacter baumannii (CRAB) isolates.MethodsIn this study we explore both genotypic and phenotypic properties of 26 CRAB isolates: 16 isolates were collected from January 2010 to March 2011, and 10 were collected between February and May 2015.ResultsWe determined that all 26 CRAB isolates possessed multiple β-lactamase genes, including genes from Groups A, C, and D. Specifically, 42% of the isolates possesses the potentially plasmid-borne genes of OXA-23-like or OXA-40-like β-lactamase. The presence of mobile gene element integron cassettes and/or integrases in 88% of the isolates suggests a possible mechanism of dissemination of antibiotic resistance genes. Additionally, the location of insertion sequence (IS) ISAba1 in promotor region of of the OXA-51-like, ADC-7, and ampC genes was confirmed. Multilocus sequence typing (MLST) demonstrated that all 26 CRAB isolates were either sequence type (ST)-229 or ST-2. Interestingly, ST-2 went from being the minority CRAB strain in the 2010–2011 isolates to the predominant strain in the 2015 isolates (from 32 to 90%). We show that the ST-2 strains have an enhanced ability to produce biofilms in comparison to the ST-229 strains, and this fact has potentially led to more successful colonization of the clinical environment over time.ConclusionsThis study provides a longitudinal genetic and phenotypic survey of two CRAB sequence types, and suggests how their differing phenotypes may interact with the selective pressures of a hospital setting effecting strain dominance over a 5-year period. More... »

PAGES

75

Journal

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12941-017-0248-3

DOI

http://dx.doi.org/10.1186/s12941-017-0248-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1092710354

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29145853


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39 schema:description BackgroundAcinetobacter baumannii is a nosocomial pathogen which is establishing as a major cause of morbidity and mortality within the healthcare community. The success of this pathogen is largely due to its ability to rapidly gain resistance to antimicrobial therapies and its capability to persist in an abiotic environment through the production of a biofilm. Our tertiary-care hospital has showed high incidence of carbapenem-resistant Acinetobacter baumannii (CRAB) isolates.MethodsIn this study we explore both genotypic and phenotypic properties of 26 CRAB isolates: 16 isolates were collected from January 2010 to March 2011, and 10 were collected between February and May 2015.ResultsWe determined that all 26 CRAB isolates possessed multiple β-lactamase genes, including genes from Groups A, C, and D. Specifically, 42% of the isolates possesses the potentially plasmid-borne genes of OXA-23-like or OXA-40-like β-lactamase. The presence of mobile gene element integron cassettes and/or integrases in 88% of the isolates suggests a possible mechanism of dissemination of antibiotic resistance genes. Additionally, the location of insertion sequence (IS) ISAba1 in promotor region of of the OXA-51-like, ADC-7, and ampC genes was confirmed. Multilocus sequence typing (MLST) demonstrated that all 26 CRAB isolates were either sequence type (ST)-229 or ST-2. Interestingly, ST-2 went from being the minority CRAB strain in the 2010–2011 isolates to the predominant strain in the 2015 isolates (from 32 to 90%). We show that the ST-2 strains have an enhanced ability to produce biofilms in comparison to the ST-229 strains, and this fact has potentially led to more successful colonization of the clinical environment over time.ConclusionsThis study provides a longitudinal genetic and phenotypic survey of two CRAB sequence types, and suggests how their differing phenotypes may interact with the selective pressures of a hospital setting effecting strain dominance over a 5-year period.
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45 schema:keywords ADC-7
46 Acinetobacter baumannii
47 CRAB isolates
48 ConclusionsThis study
49 D. Specifically
50 ISAba1
51 MethodsIn
52 ST-2
53 Specifically
54 ability
55 abiotic environment
56 antibiotic resistance genes
57 antimicrobial therapy
58 baumannii
59 biofilms
60 capability
61 carbapenem-resistant Acinetobacter baumannii
62 cause
63 characterization
64 clinical environment
65 clinical isolates
66 colonization
67 community
68 comparison
69 dissemination
70 dominance
71 enhanced ability
72 environment
73 fact
74 genes
75 group A
76 healthcare community
77 high incidence
78 hospital
79 incidence
80 insertion sequence ISAba1
81 integrases
82 integrons
83 isolates
84 location
85 major cause
86 mechanism
87 molecular characterization
88 morbidity
89 mortality
90 multilocus sequence typing
91 multiple β-lactamase genes
92 nosocomial pathogen
93 pathogens
94 period
95 phenotype
96 phenotypic properties
97 phenotypic survey
98 plasmid-borne genes
99 possible mechanism
100 predominant strain
101 presence
102 pressure
103 production
104 promotor region
105 properties
106 region
107 resistance
108 resistance genes
109 selective pressure
110 sequence types
111 sequence typing
112 strain dominance
113 strains
114 study
115 success
116 successful colonization
117 survey
118 tertiary care hospital
119 therapy
120 time
121 types
122 typing
123 β-lactamase
124 β-lactamase genes
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