Streamlined SMFA and mosquito dark-feeding regime significantly improve malaria transmission-blocking assay robustness and sensitivity View Full Text


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Article Info

DATE

2019-01-25

AUTHORS

Tibebu Habtewold, Sofia Tapanelli, Ellen K. G. Masters, Astrid Hoermann, Nikolai Windbichler, George K. Christophides

ABSTRACT

BACKGROUND: The development of malaria transmission-blocking strategies including the generation of malaria refractory mosquitoes to replace the wild populations through means of gene drives hold great promise. The standard membrane feeding assay (SMFA) that involves mosquito feeding on parasitized blood through an artificial membrane system is a vital tool for evaluating the efficacy of transmission-blocking interventions. However, despite the availability of several published protocols, the SMFA remains highly variable and broadly insensitive. METHODS: The SMFA protocol was optimized through coordinated culturing of Anopheles coluzzii mosquitoes and Plasmodium falciparum parasite coupled with placing mosquitoes under a strict dark regime before, during, and after the gametocyte feed. RESULTS: A detailed description of essential steps is provided toward synchronized generation of highly fit An. coluzzii mosquitoes and P. falciparum gametocytes in preparation for an SMFA. A dark-infection regime that emulates the natural vector-parasite interaction system is described, which results in a significant increase in the infection intensity and prevalence. Using this optimal SMFA pipeline, a series of putative transmission-blocking antimicrobial peptides (AMPs) were screened, confirming that melittin and magainin can interfere with P. falciparum development in the vector. CONCLUSION: A robust SMFA protocol that enhances the evaluation of interventions targeting human malaria transmission in laboratory setting is reported. Melittin and magainin are identified as highly potent antiparasitic AMPs that can be used for the generation of refractory Anopheles gambiae mosquitoes. More... »

PAGES

24

References to SciGraph publications

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  • 2013-12-10. Larval nutrition differentially affects adult fitness and Plasmodium development in the malaria vectors Anopheles gambiae and Anopheles stephensi in PARASITES & VECTORS
  • 2016-09-09. An inter-laboratory comparison of standard membrane-feeding assays for evaluation of malaria transmission-blocking vaccines in MALARIA JOURNAL
  • 2015-11-05. 2A self-cleaving peptide-based multi-gene expression system in the silkworm Bombyx mori in SCIENTIFIC REPORTS
  • 2015-05-22. Malaria medicines: a glass half full? in NATURE REVIEWS DRUG DISCOVERY
  • 2016-01-25. Systematic Review: Insight into Antimalarial Peptide in INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
  • 2017-05-02. The development of sexual stage malaria gametocytes in a Wave Bioreactor in PARASITES & VECTORS
  • 2018-03-20. The tripartite interactions between the mosquito, its microbiota and Plasmodium in PARASITES & VECTORS
  • 1968-04. Possible Use of Translocations to fix Desirable Genes in Insect Pest Populations in NATURE
  • 2012-08-25. Assessing Transmission Blockade in Plasmodium spp. in MALARIA
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12936-019-2663-8

    DOI

    http://dx.doi.org/10.1186/s12936-019-2663-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1111662062

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30683107


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    100 promise
    101 protocol
    102 putative transmission-blocking antimicrobial peptides
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    106 robust SMFA protocol
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    108 sensitivity
    109 series
    110 setting
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    112 standard membrane feeding
    113 step
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    116 synchronized generation
    117 system
    118 tool
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