Structure of Rhoptry Neck Protein 2 is essential for the interaction in vitro with Apical Membrane Antigen 1 in Plasmodium ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Perla Salgado-Mejias, Flavio L. Alves, Kátia S. Françoso, Karin A. Riske, Emerson R. Silva, Antonio Miranda, Irene S. Soares

ABSTRACT

BACKGROUND: In several Apicomplexa, the formation of moving junctions (MJs) at the interface between the external membranes of the invading parasite and the host cell is essential for the process of parasite invasion. In Plasmodium falciparum and Toxoplasma gondii, the MJ is composed of the Apical Membrane Antigen 1 (AMA1) and Rhoptry Neck Proteins (RONs) complex; specifically, AMA1 interacts with RON2 during host cell invasion. METHODS: Recombinant proteins based on Plasmodium vivax RON2 (A2033-P2100) and its synthetic peptide fragments, one cyclic and one linear, based on PvRON2 (D2035-T2074) were generated and used to evaluate the interaction with P. vivax AMA1 (PvAMA1) by the far western blot, surface plasmon resonance (SPR), and isothermal titration microcalorimetry (ITC) methods. The structural studies of peptides were performed by circular dichroism, and the structural analysis of the complex of PvAMA1 with peptides based on PvRON2 (D2035-T2074) was conducted with small-angle X-ray scattering (SAXS). RESULTS: Surface plasmon resonance (KD = 23.91 ± 2.078 μmol/L) and ITC (K = 3 × 105 mol/L) studies conclusively showed an interaction between the cyclic peptide based on PvRON2 and PvAMA1-His6. In contrast, the linear peptide and recombinant PvRON2 (GST fusion protein) did not show an interaction with PvAMA1. However, the interaction among recombinant proteins PvRON2.2 and PvAMA1-His6 was possible to show by far western blot. CONCLUSIONS: The results show that the PvRON2 structure, particularly the S-S bond between C2051 and C2063, is determinant for the existence of the interaction between PvAMA1 and PvRON2. More... »

PAGES

25

Identifiers

URI

http://scigraph.springernature.com/pub.10.1186/s12936-019-2649-6

DOI

http://dx.doi.org/10.1186/s12936-019-2649-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111658737

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30683104


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of La Frontera", 
          "id": "https://www.grid.ac/institutes/grid.412163.3", 
          "name": [
            "Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo, SP, Brazil", 
            "Department of Chemical Sciences and Natural Resources, Faculty of Engineering and Science, University of La Frontera, Temuco, Chile"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Salgado-Mejias", 
        "givenName": "Perla", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Sao Paulo", 
          "id": "https://www.grid.ac/institutes/grid.411249.b", 
          "name": [
            "Departamento de Biof\u00edsica, Universidade Federal de S\u00e3o Paulo, S\u00e3o Paulo, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Alves", 
        "givenName": "Flavio L.", 
        "id": "sg:person.0641645700.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641645700.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Sao Paulo", 
          "id": "https://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fran\u00e7oso", 
        "givenName": "K\u00e1tia S.", 
        "id": "sg:person.0654031122.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654031122.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Sao Paulo", 
          "id": "https://www.grid.ac/institutes/grid.411249.b", 
          "name": [
            "Departamento de Biof\u00edsica, Universidade Federal de S\u00e3o Paulo, S\u00e3o Paulo, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Riske", 
        "givenName": "Karin A.", 
        "id": "sg:person.01005453373.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005453373.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Sao Paulo", 
          "id": "https://www.grid.ac/institutes/grid.411249.b", 
          "name": [
            "Departamento de Biof\u00edsica, Universidade Federal de S\u00e3o Paulo, S\u00e3o Paulo, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Silva", 
        "givenName": "Emerson R.", 
        "id": "sg:person.01212263304.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01212263304.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Sao Paulo", 
          "id": "https://www.grid.ac/institutes/grid.411249.b", 
          "name": [
            "Departamento de Biof\u00edsica, Universidade Federal de S\u00e3o Paulo, S\u00e3o Paulo, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miranda", 
        "givenName": "Antonio", 
        "id": "sg:person.01204557425.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204557425.94"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Sao Paulo", 
          "id": "https://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Soares", 
        "givenName": "Irene S.", 
        "id": "sg:person.01154630736.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154630736.77"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1371/journal.ppat.1001276", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000674463"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/iai.01169-13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001035369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s002188989100081x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002181194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1085/jgp.201010508", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003073627"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vaccine.2010.07.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004051231"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/chir.10153", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006785869"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi011043a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009059228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi011043a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009059228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pt.2012.01.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013427731"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/14760584.2016.1236689", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013716837"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/14760584.2016.1236689", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013716837"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/14760584.2016.1236689", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013716837"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1409928111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015054511"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ijpara.2016.09.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017301707"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pt.2013.03.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021447724"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07327", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022364060", 
          "https://doi.org/10.1038/nature07327"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ijpara.2004.11.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023446312"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2875-11-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024029582", 
          "https://doi.org/10.1186/1475-2875-11-11"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms3261", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024177574", 
          "https://doi.org/10.1038/ncomms3261"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1110303108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025430856"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/9781118120392.ch15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028638070"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2007.459", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029258323", 
          "https://doi.org/10.1038/nprot.2007.459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0021889809000338", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030433643"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.ppat.1002755", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033142820"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/iai.05048-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036571401"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1590/s0074-02762007005000040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039343787"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1462-5822.2011.01597.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041314520"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m109.080770", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046983101"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.micinf.2008.07.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049975258"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(96)01279-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053038111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1204988", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062464662"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4269/ajtmh.2006.75.582", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077308792"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0183198", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091229449"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/cmi.12835", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101247519"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "BACKGROUND: In several Apicomplexa, the formation of moving junctions (MJs) at the interface between the external membranes of the invading parasite and the host cell is essential for the process of parasite invasion. In Plasmodium falciparum and Toxoplasma gondii, the MJ is composed of the Apical Membrane Antigen 1 (AMA1) and Rhoptry Neck Proteins (RONs) complex; specifically, AMA1 interacts with RON2 during host cell invasion.\nMETHODS: Recombinant proteins based on Plasmodium vivax RON2 (A2033-P2100) and its synthetic peptide fragments, one cyclic and one linear, based on PvRON2 (D2035-T2074) were generated and used to evaluate the interaction with P. vivax AMA1 (PvAMA1) by the far western blot, surface plasmon resonance (SPR), and isothermal titration microcalorimetry (ITC) methods. The structural studies of peptides were performed by circular dichroism, and the structural analysis of the complex of PvAMA1 with peptides based on PvRON2 (D2035-T2074) was conducted with small-angle X-ray scattering (SAXS).\nRESULTS: Surface plasmon resonance (KD\u2009=\u200923.91\u2009\u00b1\u20092.078\u00a0\u03bcmol/L) and ITC (K\u2009=\u20093\u00a0\u00d7\u00a0105 mol/L) studies conclusively showed an interaction between the cyclic peptide based on PvRON2 and PvAMA1-His6. In contrast, the linear peptide and recombinant PvRON2 (GST fusion protein) did not show an interaction with PvAMA1. However, the interaction among recombinant proteins PvRON2.2 and PvAMA1-His6 was possible to show by far western blot.\nCONCLUSIONS: The results show that the PvRON2 structure, particularly the S-S bond between C2051 and C2063, is determinant for the existence of the interaction between PvAMA1 and PvRON2.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1186/s12936-019-2649-6", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.4478566", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.4480548", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1030597", 
        "issn": [
          "1475-2875"
        ], 
        "name": "Malaria Journal", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "18"
      }
    ], 
    "name": "Structure of Rhoptry Neck Protein 2 is essential for the interaction in vitro with Apical Membrane Antigen 1 in Plasmodium vivax", 
    "pagination": "25", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "6db2bf52b89744603a5b9e6719cef2e603d1c93b84763ba95715394a376bc0d8"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30683104"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101139802"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1186/s12936-019-2649-6"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1111658737"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1186/s12936-019-2649-6", 
      "https://app.dimensions.ai/details/publication/pub.1111658737"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T08:59", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000326_0000000326/records_68477_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1186%2Fs12936-019-2649-6"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12936-019-2649-6'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12936-019-2649-6'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12936-019-2649-6'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12936-019-2649-6'


 

This table displays all metadata directly associated to this object as RDF triples.

217 TRIPLES      21 PREDICATES      60 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1186/s12936-019-2649-6 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N9b72f7dfeb944da38e196e78c78ad51b
4 schema:citation sg:pub.10.1038/nature07327
5 sg:pub.10.1038/ncomms3261
6 sg:pub.10.1038/nprot.2007.459
7 sg:pub.10.1186/1475-2875-11-11
8 https://doi.org/10.1002/9781118120392.ch15
9 https://doi.org/10.1002/chir.10153
10 https://doi.org/10.1016/j.ijpara.2004.11.003
11 https://doi.org/10.1016/j.ijpara.2016.09.006
12 https://doi.org/10.1016/j.micinf.2008.07.023
13 https://doi.org/10.1016/j.pt.2012.01.005
14 https://doi.org/10.1016/j.pt.2013.03.012
15 https://doi.org/10.1016/j.vaccine.2010.07.017
16 https://doi.org/10.1016/s0014-5793(96)01279-3
17 https://doi.org/10.1021/bi011043a
18 https://doi.org/10.1073/pnas.1110303108
19 https://doi.org/10.1073/pnas.1409928111
20 https://doi.org/10.1074/jbc.m109.080770
21 https://doi.org/10.1080/14760584.2016.1236689
22 https://doi.org/10.1085/jgp.201010508
23 https://doi.org/10.1107/s0021889809000338
24 https://doi.org/10.1107/s002188989100081x
25 https://doi.org/10.1111/cmi.12835
26 https://doi.org/10.1111/j.1462-5822.2011.01597.x
27 https://doi.org/10.1126/science.1204988
28 https://doi.org/10.1128/iai.01169-13
29 https://doi.org/10.1128/iai.05048-11
30 https://doi.org/10.1371/journal.pone.0183198
31 https://doi.org/10.1371/journal.ppat.1001276
32 https://doi.org/10.1371/journal.ppat.1002755
33 https://doi.org/10.1590/s0074-02762007005000040
34 https://doi.org/10.4269/ajtmh.2006.75.582
35 schema:datePublished 2019-12
36 schema:datePublishedReg 2019-12-01
37 schema:description BACKGROUND: In several Apicomplexa, the formation of moving junctions (MJs) at the interface between the external membranes of the invading parasite and the host cell is essential for the process of parasite invasion. In Plasmodium falciparum and Toxoplasma gondii, the MJ is composed of the Apical Membrane Antigen 1 (AMA1) and Rhoptry Neck Proteins (RONs) complex; specifically, AMA1 interacts with RON2 during host cell invasion. METHODS: Recombinant proteins based on Plasmodium vivax RON2 (A2033-P2100) and its synthetic peptide fragments, one cyclic and one linear, based on PvRON2 (D2035-T2074) were generated and used to evaluate the interaction with P. vivax AMA1 (PvAMA1) by the far western blot, surface plasmon resonance (SPR), and isothermal titration microcalorimetry (ITC) methods. The structural studies of peptides were performed by circular dichroism, and the structural analysis of the complex of PvAMA1 with peptides based on PvRON2 (D2035-T2074) was conducted with small-angle X-ray scattering (SAXS). RESULTS: Surface plasmon resonance (KD = 23.91 ± 2.078 μmol/L) and ITC (K = 3 × 105 mol/L) studies conclusively showed an interaction between the cyclic peptide based on PvRON2 and PvAMA1-His6. In contrast, the linear peptide and recombinant PvRON2 (GST fusion protein) did not show an interaction with PvAMA1. However, the interaction among recombinant proteins PvRON2.2 and PvAMA1-His6 was possible to show by far western blot. CONCLUSIONS: The results show that the PvRON2 structure, particularly the S-S bond between C2051 and C2063, is determinant for the existence of the interaction between PvAMA1 and PvRON2.
38 schema:genre research_article
39 schema:inLanguage en
40 schema:isAccessibleForFree true
41 schema:isPartOf N081423381fc74e2e9eb66bebc9a7eedc
42 Ne04f452c919b41838b8d1ff093a15dad
43 sg:journal.1030597
44 schema:name Structure of Rhoptry Neck Protein 2 is essential for the interaction in vitro with Apical Membrane Antigen 1 in Plasmodium vivax
45 schema:pagination 25
46 schema:productId N2703ba8674cf49c1ba77c374c2a4c3ed
47 N8ffd2dec20054a78bf294e49c9bdb745
48 Nd6f26f919770466799f799a9f79c4420
49 Ndbbe3e1cb15341fea37cb600890f5fe6
50 Neb94e89c76044c8b907792cfc14948d5
51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111658737
52 https://doi.org/10.1186/s12936-019-2649-6
53 schema:sdDatePublished 2019-04-11T08:59
54 schema:sdLicense https://scigraph.springernature.com/explorer/license/
55 schema:sdPublisher Nda2ac019f1b24285b59d8a85771bd82d
56 schema:url https://link.springer.com/10.1186%2Fs12936-019-2649-6
57 sgo:license sg:explorer/license/
58 sgo:sdDataset articles
59 rdf:type schema:ScholarlyArticle
60 N081423381fc74e2e9eb66bebc9a7eedc schema:volumeNumber 18
61 rdf:type schema:PublicationVolume
62 N1c48eb0cd39e40f9a1f74613a2399b19 rdf:first sg:person.01154630736.77
63 rdf:rest rdf:nil
64 N2703ba8674cf49c1ba77c374c2a4c3ed schema:name nlm_unique_id
65 schema:value 101139802
66 rdf:type schema:PropertyValue
67 N5a6d600ca39f4484b960e2e21706cf73 rdf:first sg:person.01204557425.94
68 rdf:rest N1c48eb0cd39e40f9a1f74613a2399b19
69 N5ae61355ef03405e8cf3ececac9a4555 rdf:first sg:person.0654031122.06
70 rdf:rest Na3631ca35ed74ff7acadaa03f90a148d
71 N774ba6012c574875a9fe6d5db52270d6 schema:affiliation https://www.grid.ac/institutes/grid.412163.3
72 schema:familyName Salgado-Mejias
73 schema:givenName Perla
74 rdf:type schema:Person
75 N7d19f35d9d62437b85e4a7d4e955c2bd rdf:first sg:person.0641645700.61
76 rdf:rest N5ae61355ef03405e8cf3ececac9a4555
77 N8db47515361f43c3b153524ffe077538 rdf:first sg:person.01212263304.52
78 rdf:rest N5a6d600ca39f4484b960e2e21706cf73
79 N8ffd2dec20054a78bf294e49c9bdb745 schema:name readcube_id
80 schema:value 6db2bf52b89744603a5b9e6719cef2e603d1c93b84763ba95715394a376bc0d8
81 rdf:type schema:PropertyValue
82 N9b72f7dfeb944da38e196e78c78ad51b rdf:first N774ba6012c574875a9fe6d5db52270d6
83 rdf:rest N7d19f35d9d62437b85e4a7d4e955c2bd
84 Na3631ca35ed74ff7acadaa03f90a148d rdf:first sg:person.01005453373.09
85 rdf:rest N8db47515361f43c3b153524ffe077538
86 Nd6f26f919770466799f799a9f79c4420 schema:name doi
87 schema:value 10.1186/s12936-019-2649-6
88 rdf:type schema:PropertyValue
89 Nda2ac019f1b24285b59d8a85771bd82d schema:name Springer Nature - SN SciGraph project
90 rdf:type schema:Organization
91 Ndbbe3e1cb15341fea37cb600890f5fe6 schema:name dimensions_id
92 schema:value pub.1111658737
93 rdf:type schema:PropertyValue
94 Ne04f452c919b41838b8d1ff093a15dad schema:issueNumber 1
95 rdf:type schema:PublicationIssue
96 Neb94e89c76044c8b907792cfc14948d5 schema:name pubmed_id
97 schema:value 30683104
98 rdf:type schema:PropertyValue
99 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
100 schema:name Biological Sciences
101 rdf:type schema:DefinedTerm
102 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
103 schema:name Biochemistry and Cell Biology
104 rdf:type schema:DefinedTerm
105 sg:grant.4478566 http://pending.schema.org/fundedItem sg:pub.10.1186/s12936-019-2649-6
106 rdf:type schema:MonetaryGrant
107 sg:grant.4480548 http://pending.schema.org/fundedItem sg:pub.10.1186/s12936-019-2649-6
108 rdf:type schema:MonetaryGrant
109 sg:journal.1030597 schema:issn 1475-2875
110 schema:name Malaria Journal
111 rdf:type schema:Periodical
112 sg:person.01005453373.09 schema:affiliation https://www.grid.ac/institutes/grid.411249.b
113 schema:familyName Riske
114 schema:givenName Karin A.
115 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005453373.09
116 rdf:type schema:Person
117 sg:person.01154630736.77 schema:affiliation https://www.grid.ac/institutes/grid.11899.38
118 schema:familyName Soares
119 schema:givenName Irene S.
120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154630736.77
121 rdf:type schema:Person
122 sg:person.01204557425.94 schema:affiliation https://www.grid.ac/institutes/grid.411249.b
123 schema:familyName Miranda
124 schema:givenName Antonio
125 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204557425.94
126 rdf:type schema:Person
127 sg:person.01212263304.52 schema:affiliation https://www.grid.ac/institutes/grid.411249.b
128 schema:familyName Silva
129 schema:givenName Emerson R.
130 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01212263304.52
131 rdf:type schema:Person
132 sg:person.0641645700.61 schema:affiliation https://www.grid.ac/institutes/grid.411249.b
133 schema:familyName Alves
134 schema:givenName Flavio L.
135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641645700.61
136 rdf:type schema:Person
137 sg:person.0654031122.06 schema:affiliation https://www.grid.ac/institutes/grid.11899.38
138 schema:familyName Françoso
139 schema:givenName Kátia S.
140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654031122.06
141 rdf:type schema:Person
142 sg:pub.10.1038/nature07327 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022364060
143 https://doi.org/10.1038/nature07327
144 rdf:type schema:CreativeWork
145 sg:pub.10.1038/ncomms3261 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024177574
146 https://doi.org/10.1038/ncomms3261
147 rdf:type schema:CreativeWork
148 sg:pub.10.1038/nprot.2007.459 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029258323
149 https://doi.org/10.1038/nprot.2007.459
150 rdf:type schema:CreativeWork
151 sg:pub.10.1186/1475-2875-11-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024029582
152 https://doi.org/10.1186/1475-2875-11-11
153 rdf:type schema:CreativeWork
154 https://doi.org/10.1002/9781118120392.ch15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028638070
155 rdf:type schema:CreativeWork
156 https://doi.org/10.1002/chir.10153 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006785869
157 rdf:type schema:CreativeWork
158 https://doi.org/10.1016/j.ijpara.2004.11.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023446312
159 rdf:type schema:CreativeWork
160 https://doi.org/10.1016/j.ijpara.2016.09.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017301707
161 rdf:type schema:CreativeWork
162 https://doi.org/10.1016/j.micinf.2008.07.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049975258
163 rdf:type schema:CreativeWork
164 https://doi.org/10.1016/j.pt.2012.01.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013427731
165 rdf:type schema:CreativeWork
166 https://doi.org/10.1016/j.pt.2013.03.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021447724
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1016/j.vaccine.2010.07.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004051231
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1016/s0014-5793(96)01279-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053038111
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1021/bi011043a schema:sameAs https://app.dimensions.ai/details/publication/pub.1009059228
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1073/pnas.1110303108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025430856
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1073/pnas.1409928111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015054511
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1074/jbc.m109.080770 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046983101
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1080/14760584.2016.1236689 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013716837
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1085/jgp.201010508 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003073627
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1107/s0021889809000338 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030433643
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1107/s002188989100081x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002181194
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1111/cmi.12835 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101247519
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1111/j.1462-5822.2011.01597.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1041314520
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1126/science.1204988 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062464662
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1128/iai.01169-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001035369
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1128/iai.05048-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036571401
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1371/journal.pone.0183198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091229449
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1371/journal.ppat.1001276 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000674463
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1371/journal.ppat.1002755 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033142820
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1590/s0074-02762007005000040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039343787
205 rdf:type schema:CreativeWork
206 https://doi.org/10.4269/ajtmh.2006.75.582 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077308792
207 rdf:type schema:CreativeWork
208 https://www.grid.ac/institutes/grid.11899.38 schema:alternateName University of Sao Paulo
209 schema:name Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil
210 rdf:type schema:Organization
211 https://www.grid.ac/institutes/grid.411249.b schema:alternateName Federal University of Sao Paulo
212 schema:name Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, SP, Brazil
213 rdf:type schema:Organization
214 https://www.grid.ac/institutes/grid.412163.3 schema:alternateName University of La Frontera
215 schema:name Department of Chemical Sciences and Natural Resources, Faculty of Engineering and Science, University of La Frontera, Temuco, Chile
216 Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil
217 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...