Bead-based assays to simultaneously detect multiple human inherited blood disorders associated with malaria View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Lynn Grignard, Catherine Mair, Jonathan Curry, Laleta Mahey, Guide J. H. Bastiaens, Alfred B. Tiono, Joseph Okebe, Sam A. Coulibaly, Bronner P. Gonçalves, Muna Affara, Alphonse Ouédraogo, Edith C. Bougouma, Guillaume S. Sanou, Issa Nébié, Kjerstin H. W. Lanke, Sodiomon B. Sirima, Umberto d’Alessandro, Taane G. Clark, Susana Campino, Teun Bousema, Chris Drakeley

ABSTRACT

BACKGROUND: Glucose-6-phosphate dehydrogenase deficiency (G6PDd), haemoglobin C (HbC) and S (HbS) are inherited blood disorders (IBD) common in populations in malaria endemic areas. All are associated to some degree with protection against clinical malaria whilst additionally G6PDd is associated with haemolysis following treatment with 8-aminoquinolines. Measuring the prevalence of these inherited blood disorders in affected populations can improve understanding of disease epidemiology. Current methodologies in epidemiological studies commonly rely on individual target amplification and visualization; here a method is presented to simultaneously detect the polymorphisms and that can be expanded to include other single nucleotide polymorphisms (SNPs) of interest. METHODS: Human DNA from whole blood samples was amplified in a novel, multiplex PCR reaction and extended with SNP-specific probes in an allele specific primer extension (ASPE) to simultaneously detect four epidemiologically important human markers including G6PD SNPs (G202A and A376G) and common haemoglobin mutations (HbS and HbC). The products were hybridized to magnetic beads and the median fluorescence intensity (MFI) was read on MAGPIX® (Luminex corp.). Genotyping data was compared to phenotypical data generated by flow cytometry and to established genotyping methods. RESULTS: Seventy-five samples from Burkina Faso (n = 75/78, 96.2%) and 58 samples from The Gambia (n = 58/61, 95.1%) had a G6PD and a HBB genotype successfully assigned by the bead-based assay. Flow cytometry data available for n = 61 samples further supported the concordance between % G6PD normal/deficient cells and genotype. CONCLUSIONS: The bead based assay compares well to alternative measures of genotyping and phenotyping for G6PD. The screening is high throughput, adaptable to inclusion of multiple targets of interest and easily standardized. More... »

PAGES

14

References to SciGraph publications

  • 2010-04. Genetic variation in human HBB is associated with Plasmodium falciparum transmission in NATURE GENETICS
  • 2012-12. A novel cytofluorometric assay for the detection and quantification of glucose-6-phosphate dehydrogenase deficiency in SCIENTIFIC REPORTS
  • 1986-06. High frequencies of α-thalassaemia are the result of natural selection by malaria in NATURE
  • 2009-08. Allelic heterogeneity of G6PD deficiency in West Africa and severe malaria susceptibility in EUROPEAN JOURNAL OF HUMAN GENETICS
  • 2018-12. Haemolysis and haem oxygenase-1 induction during persistent “asymptomatic” malaria infection in Burkinabé children in MALARIA JOURNAL
  • 2018-12. Single low-dose primaquine for blocking transmission of Plasmodium falciparum malaria – a proposed model-derived age-based regimen for sub-Saharan Africa in BMC MEDICINE
  • 2010-11. Global distribution of the sickle cell gene and geographical confirmation of the malaria hypothesis in NATURE COMMUNICATIONS
  • 2001-11. Haemoglobin C protects against clinical Plasmodium falciparum malaria in NATURE
  • 2017-12. G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon in MALARIA JOURNAL
  • 2017-12. Hemoglobin variants shape the distribution of malaria parasites in human populations and their transmission potential in SCIENTIFIC REPORTS
  • 2014-12. The prevalence of glucose-6-phosphate dehydrogenase deficiency in Gambian school children in MALARIA JOURNAL
  • 2013-12. Spatial distribution of G6PD deficiency variants across malaria-endemic regions in MALARIA JOURNAL
  • 2013-12. Cryopreservation of glucose-6-phosphate dehydrogenase activity inside red blood cells: developing a specimen repository in support of development and evaluation of glucose-6-phosphate dehydrogenase deficiency tests in MALARIA JOURNAL
  • 2013-12. The distribution of haemoglobin C and its prevalence in newborns in Africa in SCIENTIFIC REPORTS
  • 2016-12. Sickle haemoglobin, haemoglobin C and malaria mortality feedbacks in MALARIA JOURNAL
  • 2017-12. The G6PD flow-cytometric assay is a reliable tool for diagnosis of G6PD deficiency in women and anaemic subjects in SCIENTIFIC REPORTS
  • 2015-12. Glucose-6-phosphate dehydrogenase deficiency among malaria patients of Honduras: a descriptive study of archival blood samples in MALARIA JOURNAL
  • 2018-12. Primaquine ineligibility in anti-relapse therapy of Plasmodium vivax malaria: the problem of G6PD deficiency and cytochrome P-450 2D6 polymorphisms in MALARIA JOURNAL
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1186/s12936-019-2648-7

    DOI

    http://dx.doi.org/10.1186/s12936-019-2648-7

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1111576835

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30665411


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Adolescent", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Adult", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Anemia, Sickle Cell", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Burkina Faso", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Child", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genotyping Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Glucosephosphate Dehydrogenase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Glucosephosphate Dehydrogenase Deficiency", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hemoglobin C", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hemoglobin C Disease", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hemoglobin, Sickle", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Malaria", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Male", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Middle Aged", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymorphism, Single Nucleotide", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Young Adult", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "London School of Hygiene & Tropical Medicine", 
              "id": "https://www.grid.ac/institutes/grid.8991.9", 
              "name": [
                "Department of Immunology and Infection, London School of Hygiene & Tropical Medicine, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Grignard", 
            "givenName": "Lynn", 
            "id": "sg:person.01163442506.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163442506.23"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "London School of Hygiene & Tropical Medicine", 
              "id": "https://www.grid.ac/institutes/grid.8991.9", 
              "name": [
                "Department of Immunology and Infection, London School of Hygiene & Tropical Medicine, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mair", 
            "givenName": "Catherine", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "LGC Genomics, Hoddesdon, Hertfordshire, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Curry", 
            "givenName": "Jonathan", 
            "id": "sg:person.011004004353.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011004004353.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "LGC Genomics, Hoddesdon, Hertfordshire, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mahey", 
            "givenName": "Laleta", 
            "id": "sg:person.011347720010.59", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011347720010.59"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Radboud University Nijmegen Medical Centre", 
              "id": "https://www.grid.ac/institutes/grid.10417.33", 
              "name": [
                "Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bastiaens", 
            "givenName": "Guide J. H.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "MalariaGEN", 
              "id": "https://www.grid.ac/institutes/grid.418150.9", 
              "name": [
                "Department of Biomedical Sciences, Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tiono", 
            "givenName": "Alfred B.", 
            "id": "sg:person.01010116700.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010116700.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Disease Control & Elimination Theme, Medical Research Council Unit at London School of Hygiene and Tropical Medicine, Fajara, The Gambia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Okebe", 
            "givenName": "Joseph", 
            "id": "sg:person.01313520764.52", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313520764.52"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "MalariaGEN", 
              "id": "https://www.grid.ac/institutes/grid.418150.9", 
              "name": [
                "Department of Biomedical Sciences, Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Coulibaly", 
            "givenName": "Sam A.", 
            "id": "sg:person.01240573700.51", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240573700.51"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "London School of Hygiene & Tropical Medicine", 
              "id": "https://www.grid.ac/institutes/grid.8991.9", 
              "name": [
                "Department of Immunology and Infection, London School of Hygiene & Tropical Medicine, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gon\u00e7alves", 
            "givenName": "Bronner P.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Disease Control & Elimination Theme, Medical Research Council Unit at London School of Hygiene and Tropical Medicine, Fajara, The Gambia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Affara", 
            "givenName": "Muna", 
            "id": "sg:person.01143433256.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143433256.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "MalariaGEN", 
              "id": "https://www.grid.ac/institutes/grid.418150.9", 
              "name": [
                "Department of Biomedical Sciences, Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ou\u00e9draogo", 
            "givenName": "Alphonse", 
            "id": "sg:person.013056523777.51", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013056523777.51"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "MalariaGEN", 
              "id": "https://www.grid.ac/institutes/grid.418150.9", 
              "name": [
                "Department of Biomedical Sciences, Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bougouma", 
            "givenName": "Edith C.", 
            "id": "sg:person.01323101204.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01323101204.55"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "MalariaGEN", 
              "id": "https://www.grid.ac/institutes/grid.418150.9", 
              "name": [
                "Department of Biomedical Sciences, Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sanou", 
            "givenName": "Guillaume S.", 
            "id": "sg:person.01101047413.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101047413.39"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "MalariaGEN", 
              "id": "https://www.grid.ac/institutes/grid.418150.9", 
              "name": [
                "Department of Biomedical Sciences, Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "N\u00e9bi\u00e9", 
            "givenName": "Issa", 
            "id": "sg:person.0635025104.24", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0635025104.24"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Radboud University Nijmegen Medical Centre", 
              "id": "https://www.grid.ac/institutes/grid.10417.33", 
              "name": [
                "Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lanke", 
            "givenName": "Kjerstin H. W.", 
            "id": "sg:person.01137322113.00", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137322113.00"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "MalariaGEN", 
              "id": "https://www.grid.ac/institutes/grid.418150.9", 
              "name": [
                "Department of Biomedical Sciences, Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sirima", 
            "givenName": "Sodiomon B.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "London School of Hygiene & Tropical Medicine", 
              "id": "https://www.grid.ac/institutes/grid.8991.9", 
              "name": [
                "Disease Control & Elimination Theme, Medical Research Council Unit at London School of Hygiene and Tropical Medicine, Fajara, The Gambia", 
                "Department of Disease Control, London School of Hygiene and Tropical Medicine, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "d\u2019Alessandro", 
            "givenName": "Umberto", 
            "id": "sg:person.01063334514.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063334514.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "London School of Hygiene & Tropical Medicine", 
              "id": "https://www.grid.ac/institutes/grid.8991.9", 
              "name": [
                "Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, London, UK", 
                "Department of Infectious Disease Epidemiology, London School of Hygiene and Tropical Medicine, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Clark", 
            "givenName": "Taane G.", 
            "id": "sg:person.010201615642.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010201615642.23"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "London School of Hygiene & Tropical Medicine", 
              "id": "https://www.grid.ac/institutes/grid.8991.9", 
              "name": [
                "Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Campino", 
            "givenName": "Susana", 
            "id": "sg:person.01320377661.56", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320377661.56"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Radboud University Nijmegen Medical Centre", 
              "id": "https://www.grid.ac/institutes/grid.10417.33", 
              "name": [
                "Department of Immunology and Infection, London School of Hygiene & Tropical Medicine, London, UK", 
                "Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bousema", 
            "givenName": "Teun", 
            "id": "sg:person.01023575701.67", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023575701.67"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "London School of Hygiene & Tropical Medicine", 
              "id": "https://www.grid.ac/institutes/grid.8991.9", 
              "name": [
                "Department of Immunology and Infection, London School of Hygiene & Tropical Medicine, London, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Drakeley", 
            "givenName": "Chris", 
            "id": "sg:person.016327373057.48", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016327373057.48"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1186/1475-2875-12-418", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004162306", 
              "https://doi.org/10.1186/1475-2875-12-418"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/321744a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006612157", 
              "https://doi.org/10.1038/321744a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/infdis/jiv098", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007915842"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35104556", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008333288", 
              "https://doi.org/10.1038/35104556"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35104556", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008333288", 
              "https://doi.org/10.1038/35104556"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-015-0823-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010212998", 
              "https://doi.org/10.1186/s12936-015-0823-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.7554/elife.15085", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013257417"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.mbs.2004.01.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013281796"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms1104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015059965", 
              "https://doi.org/10.1038/ncomms1104"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms1104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015059965", 
              "https://doi.org/10.1038/ncomms1104"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2141.1977.tb00589.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018570270"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1473-3099(13)70268-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018876102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-015-1077-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020238224", 
              "https://doi.org/10.1186/s12936-015-1077-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jcm.01683-06", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020946835"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pmed.1001339", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023509008"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng.554", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023842929", 
              "https://doi.org/10.1038/ng.554"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng.554", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023842929", 
              "https://doi.org/10.1038/ng.554"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1473-3099(15)00479-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023953646"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1473-3099(15)00479-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023953646"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1473-3099(15)00479-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023953646"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1473-3099(15)00479-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023953646"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bcmd.2012.01.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025191794"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ejhg.2009.8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025605016", 
              "https://doi.org/10.1038/ejhg.2009.8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep00299", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031129866", 
              "https://doi.org/10.1038/srep00299"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep01671", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032689339", 
              "https://doi.org/10.1038/srep01671"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/b978-0-12-407826-0.00004-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042166376"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-2875-13-148", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044080201", 
              "https://doi.org/10.1186/1475-2875-13-148"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-2875-12-286", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045318010", 
              "https://doi.org/10.1186/1475-2875-12-286"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pgen.1004960", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045872687"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jcm.00904-13", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050462485"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1369/0022155415580594", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050698334"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1369/0022155415580594", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050698334"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1086/338628", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1058637905"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0169930", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1074215508"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2144/01303dd04", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1074784217"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076027614", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080987014", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082484015", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4269/ajtmh.1996.54.111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1082882080"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-017-1889-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1086039400", 
              "https://doi.org/10.1186/s12936-017-1889-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-017-1889-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1086039400", 
              "https://doi.org/10.1186/s12936-017-1889-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4269/ajtmh.16-0779", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090917394"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4269/ajtmh.16-0779", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090917394"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-017-10045-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091331754", 
              "https://doi.org/10.1038/s41598-017-10045-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-017-10045-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091331754", 
              "https://doi.org/10.1038/s41598-017-10045-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-017-14627-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092371234", 
              "https://doi.org/10.1038/s41598-017-14627-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0190272", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1100338217"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12916-017-0990-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1100473369", 
              "https://doi.org/10.1186/s12916-017-0990-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-018-2190-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1100555169", 
              "https://doi.org/10.1186/s12936-018-2190-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pntd.0006230", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1103466211"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pntd.0006440", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1103488674"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-018-2402-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1105341291", 
              "https://doi.org/10.1186/s12936-018-2402-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12936-018-2402-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1105341291", 
              "https://doi.org/10.1186/s12936-018-2402-6"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2019-12", 
        "datePublishedReg": "2019-12-01", 
        "description": "BACKGROUND: Glucose-6-phosphate dehydrogenase deficiency (G6PDd), haemoglobin C (HbC) and S (HbS) are inherited blood disorders (IBD) common in populations in malaria endemic areas. All are associated to some degree with protection against clinical malaria whilst additionally G6PDd is associated with haemolysis following treatment with 8-aminoquinolines. Measuring the prevalence of these inherited blood disorders in affected populations can improve understanding of disease epidemiology. Current methodologies in epidemiological studies commonly rely on individual target amplification and visualization; here a method is presented to simultaneously detect the polymorphisms and that can be expanded to include other single nucleotide polymorphisms (SNPs) of interest.\nMETHODS: Human DNA from whole blood samples was amplified in a novel, multiplex PCR reaction and extended with SNP-specific probes in an allele specific primer extension (ASPE) to simultaneously detect four epidemiologically important human markers including G6PD SNPs (G202A and A376G) and common haemoglobin mutations (HbS and HbC). The products were hybridized to magnetic beads and the median fluorescence intensity (MFI) was read on MAGPIX\u00ae (Luminex corp.). Genotyping data was compared to phenotypical data generated by flow cytometry and to established genotyping methods.\nRESULTS: Seventy-five samples from Burkina Faso (n\u2009=\u200975/78, 96.2%) and 58 samples from The Gambia (n\u2009=\u200958/61, 95.1%) had a G6PD and a HBB genotype successfully assigned by the bead-based assay. Flow cytometry data available for n\u2009=\u200961 samples further supported the concordance between % G6PD normal/deficient cells and genotype.\nCONCLUSIONS: The bead based assay compares well to alternative measures of genotyping and phenotyping for G6PD. The screening is high throughput, adaptable to inclusion of multiple targets of interest and easily standardized.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1186/s12936-019-2648-7", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2751489", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.6583834", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.7389855", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1030597", 
            "issn": [
              "1475-2875"
            ], 
            "name": "Malaria Journal", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "18"
          }
        ], 
        "name": "Bead-based assays to simultaneously detect multiple human inherited blood disorders associated with malaria", 
        "pagination": "14", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "dc083f304170f94b1b7a88f9acde513678656e12f152b3223c5eae917e8bff5f"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30665411"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101139802"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1186/s12936-019-2648-7"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1111576835"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1186/s12936-019-2648-7", 
          "https://app.dimensions.ai/details/publication/pub.1111576835"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T11:02", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000352_0000000352/records_60351_00000003.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://link.springer.com/10.1186%2Fs12936-019-2648-7"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1186/s12936-019-2648-7'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1186/s12936-019-2648-7'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1186/s12936-019-2648-7'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1186/s12936-019-2648-7'


     

    This table displays all metadata directly associated to this object as RDF triples.

    438 TRIPLES      21 PREDICATES      88 URIs      38 LITERALS      26 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1186/s12936-019-2648-7 schema:about N178603b32c2a4b838963c61f8a691765
    2 N27dcb931fe4c42f385e2c12714212f2d
    3 N2b970bccc0f7459da9c361faf3d3d233
    4 N36c0e9e62245498d8216913d849ca594
    5 N3fc992a665384828ac51b80a7fba09d2
    6 N4491d45f674048df919665ade6973ca3
    7 N4a0f19504433411691590130d1d3e089
    8 N6c6c46cc016b4ff19eca768e1bc6fe40
    9 N7bc8fc00e25f439f8a4e6a2c814b0b4f
    10 N7fe14e2ea4284d5a8eabfbcf2ea1a9d2
    11 N8f0bbde7d33c43ff8271571233ea8968
    12 N93f07af1da7e4f63b1934542937adc6d
    13 Nbbee0f97e3f94e7ea8bc9ee4400a7c03
    14 Nbf0f30f4fc224807879634fc11771a16
    15 Nbf4fbb692b334aa6b912725abb33d61e
    16 Ne4220ced7a81449ab83d39d03a4841be
    17 Ne94161fe21df44cd8b67ab1ed56de2c3
    18 anzsrc-for:06
    19 anzsrc-for:0604
    20 schema:author N955ae6f0f418472398d64042076f3abc
    21 schema:citation sg:pub.10.1038/321744a0
    22 sg:pub.10.1038/35104556
    23 sg:pub.10.1038/ejhg.2009.8
    24 sg:pub.10.1038/ncomms1104
    25 sg:pub.10.1038/ng.554
    26 sg:pub.10.1038/s41598-017-10045-2
    27 sg:pub.10.1038/s41598-017-14627-y
    28 sg:pub.10.1038/srep00299
    29 sg:pub.10.1038/srep01671
    30 sg:pub.10.1186/1475-2875-12-286
    31 sg:pub.10.1186/1475-2875-12-418
    32 sg:pub.10.1186/1475-2875-13-148
    33 sg:pub.10.1186/s12916-017-0990-6
    34 sg:pub.10.1186/s12936-015-0823-z
    35 sg:pub.10.1186/s12936-015-1077-5
    36 sg:pub.10.1186/s12936-017-1889-6
    37 sg:pub.10.1186/s12936-018-2190-z
    38 sg:pub.10.1186/s12936-018-2402-6
    39 https://app.dimensions.ai/details/publication/pub.1076027614
    40 https://app.dimensions.ai/details/publication/pub.1080987014
    41 https://app.dimensions.ai/details/publication/pub.1082484015
    42 https://doi.org/10.1016/b978-0-12-407826-0.00004-7
    43 https://doi.org/10.1016/j.bcmd.2012.01.001
    44 https://doi.org/10.1016/j.mbs.2004.01.005
    45 https://doi.org/10.1016/s1473-3099(13)70268-8
    46 https://doi.org/10.1016/s1473-3099(15)00479-x
    47 https://doi.org/10.1086/338628
    48 https://doi.org/10.1093/infdis/jiv098
    49 https://doi.org/10.1111/j.1365-2141.1977.tb00589.x
    50 https://doi.org/10.1128/jcm.00904-13
    51 https://doi.org/10.1128/jcm.01683-06
    52 https://doi.org/10.1369/0022155415580594
    53 https://doi.org/10.1371/journal.pgen.1004960
    54 https://doi.org/10.1371/journal.pmed.1001339
    55 https://doi.org/10.1371/journal.pntd.0006230
    56 https://doi.org/10.1371/journal.pntd.0006440
    57 https://doi.org/10.1371/journal.pone.0169930
    58 https://doi.org/10.1371/journal.pone.0190272
    59 https://doi.org/10.2144/01303dd04
    60 https://doi.org/10.4269/ajtmh.16-0779
    61 https://doi.org/10.4269/ajtmh.1996.54.111
    62 https://doi.org/10.7554/elife.15085
    63 schema:datePublished 2019-12
    64 schema:datePublishedReg 2019-12-01
    65 schema:description BACKGROUND: Glucose-6-phosphate dehydrogenase deficiency (G6PDd), haemoglobin C (HbC) and S (HbS) are inherited blood disorders (IBD) common in populations in malaria endemic areas. All are associated to some degree with protection against clinical malaria whilst additionally G6PDd is associated with haemolysis following treatment with 8-aminoquinolines. Measuring the prevalence of these inherited blood disorders in affected populations can improve understanding of disease epidemiology. Current methodologies in epidemiological studies commonly rely on individual target amplification and visualization; here a method is presented to simultaneously detect the polymorphisms and that can be expanded to include other single nucleotide polymorphisms (SNPs) of interest. METHODS: Human DNA from whole blood samples was amplified in a novel, multiplex PCR reaction and extended with SNP-specific probes in an allele specific primer extension (ASPE) to simultaneously detect four epidemiologically important human markers including G6PD SNPs (G202A and A376G) and common haemoglobin mutations (HbS and HbC). The products were hybridized to magnetic beads and the median fluorescence intensity (MFI) was read on MAGPIX® (Luminex corp.). Genotyping data was compared to phenotypical data generated by flow cytometry and to established genotyping methods. RESULTS: Seventy-five samples from Burkina Faso (n = 75/78, 96.2%) and 58 samples from The Gambia (n = 58/61, 95.1%) had a G6PD and a HBB genotype successfully assigned by the bead-based assay. Flow cytometry data available for n = 61 samples further supported the concordance between % G6PD normal/deficient cells and genotype. CONCLUSIONS: The bead based assay compares well to alternative measures of genotyping and phenotyping for G6PD. The screening is high throughput, adaptable to inclusion of multiple targets of interest and easily standardized.
    66 schema:genre research_article
    67 schema:inLanguage en
    68 schema:isAccessibleForFree true
    69 schema:isPartOf N876fe71f9c0a4655acd329f4c08b8c49
    70 Nddae330d703c4af48de69d3f89c6b2e6
    71 sg:journal.1030597
    72 schema:name Bead-based assays to simultaneously detect multiple human inherited blood disorders associated with malaria
    73 schema:pagination 14
    74 schema:productId N13d2f85a07cc4428b0db34f47973c6db
    75 N55da25592c844118b154845e04f68539
    76 N96fc7b9e24e04949a8646a91f327d589
    77 Ne0b10bf33bc44ab5b83f00e6d3c2955e
    78 Neb47176ce64c4e628fb0b76a0a2b68bf
    79 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111576835
    80 https://doi.org/10.1186/s12936-019-2648-7
    81 schema:sdDatePublished 2019-04-11T11:02
    82 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    83 schema:sdPublisher N7c6f8466a5cd4ce7a594742b45182c63
    84 schema:url https://link.springer.com/10.1186%2Fs12936-019-2648-7
    85 sgo:license sg:explorer/license/
    86 sgo:sdDataset articles
    87 rdf:type schema:ScholarlyArticle
    88 N0465602ca1444d94a3a256ad14f875a0 rdf:first sg:person.01320377661.56
    89 rdf:rest N47bae539508149679e043d85563d0f6a
    90 N090f20b8d48b417283974d04bd8dcb21 schema:name Disease Control & Elimination Theme, Medical Research Council Unit at London School of Hygiene and Tropical Medicine, Fajara, The Gambia
    91 rdf:type schema:Organization
    92 N13d2f85a07cc4428b0db34f47973c6db schema:name dimensions_id
    93 schema:value pub.1111576835
    94 rdf:type schema:PropertyValue
    95 N178603b32c2a4b838963c61f8a691765 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Hemoglobin, Sickle
    97 rdf:type schema:DefinedTerm
    98 N27dcb931fe4c42f385e2c12714212f2d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Humans
    100 rdf:type schema:DefinedTerm
    101 N2b970bccc0f7459da9c361faf3d3d233 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Burkina Faso
    103 rdf:type schema:DefinedTerm
    104 N330ac3d3f309499fa9169c68f68c42be rdf:first sg:person.01323101204.55
    105 rdf:rest Nc56afd9bf0464687af88eafdae0cc12d
    106 N36c0e9e62245498d8216913d849ca594 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Child
    108 rdf:type schema:DefinedTerm
    109 N38bead7a779a415986c8e73633aa9f5f rdf:first Ned6e8b16d2f14a459053d559c7cf91bd
    110 rdf:rest Ncefdd965f127415f9b1d22100d9a1bd7
    111 N3afd2998fea641eda237c8480aa7fe20 schema:name Disease Control & Elimination Theme, Medical Research Council Unit at London School of Hygiene and Tropical Medicine, Fajara, The Gambia
    112 rdf:type schema:Organization
    113 N3fc992a665384828ac51b80a7fba09d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Hemoglobin C
    115 rdf:type schema:DefinedTerm
    116 N43818bb2449243a3878391506fb252ca schema:name LGC Genomics, Hoddesdon, Hertfordshire, UK
    117 rdf:type schema:Organization
    118 N4491d45f674048df919665ade6973ca3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Hemoglobin C Disease
    120 rdf:type schema:DefinedTerm
    121 N47bae539508149679e043d85563d0f6a rdf:first sg:person.01023575701.67
    122 rdf:rest N6b3c1b249073402d9b66ba3b825c4bcc
    123 N4a0f19504433411691590130d1d3e089 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    124 schema:name Polymorphism, Single Nucleotide
    125 rdf:type schema:DefinedTerm
    126 N55da25592c844118b154845e04f68539 schema:name nlm_unique_id
    127 schema:value 101139802
    128 rdf:type schema:PropertyValue
    129 N6b3c1b249073402d9b66ba3b825c4bcc rdf:first sg:person.016327373057.48
    130 rdf:rest rdf:nil
    131 N6c6c46cc016b4ff19eca768e1bc6fe40 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Glucosephosphate Dehydrogenase Deficiency
    133 rdf:type schema:DefinedTerm
    134 N7a6df9b426ef42eb8efcfa8c4b05c8f1 schema:name LGC Genomics, Hoddesdon, Hertfordshire, UK
    135 rdf:type schema:Organization
    136 N7bc8fc00e25f439f8a4e6a2c814b0b4f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Malaria
    138 rdf:type schema:DefinedTerm
    139 N7c6f8466a5cd4ce7a594742b45182c63 schema:name Springer Nature - SN SciGraph project
    140 rdf:type schema:Organization
    141 N7fe14e2ea4284d5a8eabfbcf2ea1a9d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    142 schema:name Middle Aged
    143 rdf:type schema:DefinedTerm
    144 N876fe71f9c0a4655acd329f4c08b8c49 schema:issueNumber 1
    145 rdf:type schema:PublicationIssue
    146 N8dbc9d604e20497993fe3a2a0ce74f02 rdf:first sg:person.0635025104.24
    147 rdf:rest Nd9b166d22f6849fa9cbcfcf2747051fb
    148 N8f0bbde7d33c43ff8271571233ea8968 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name Adolescent
    150 rdf:type schema:DefinedTerm
    151 N90a1ae502c9e4819afda4e51f8ffbf9a schema:affiliation https://www.grid.ac/institutes/grid.418150.9
    152 schema:familyName Sirima
    153 schema:givenName Sodiomon B.
    154 rdf:type schema:Person
    155 N93f07af1da7e4f63b1934542937adc6d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Adult
    157 rdf:type schema:DefinedTerm
    158 N955ae6f0f418472398d64042076f3abc rdf:first sg:person.01163442506.23
    159 rdf:rest N38bead7a779a415986c8e73633aa9f5f
    160 N96e3e38e8fed4f03bbb646cfd9b429ca rdf:first N97910fb96307475eb801f46acc4973d9
    161 rdf:rest Nb983e982187240bcad195df892076ded
    162 N96fc7b9e24e04949a8646a91f327d589 schema:name readcube_id
    163 schema:value dc083f304170f94b1b7a88f9acde513678656e12f152b3223c5eae917e8bff5f
    164 rdf:type schema:PropertyValue
    165 N9719f25e57c74b578f9c4833812d9a26 rdf:first Nef3bc6b1d8814218b9610101a9e51ee8
    166 rdf:rest Nc034ce804e904114bb4ce7e0b75eb264
    167 N97910fb96307475eb801f46acc4973d9 schema:affiliation https://www.grid.ac/institutes/grid.10417.33
    168 schema:familyName Bastiaens
    169 schema:givenName Guide J. H.
    170 rdf:type schema:Person
    171 N9fae443914324f619453c08f142c00cb rdf:first sg:person.010201615642.23
    172 rdf:rest N0465602ca1444d94a3a256ad14f875a0
    173 Nb983e982187240bcad195df892076ded rdf:first sg:person.01010116700.30
    174 rdf:rest Nbe5df0f3c9604b1dbe849270ec72e94d
    175 Nbbee0f97e3f94e7ea8bc9ee4400a7c03 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    176 schema:name Anemia, Sickle Cell
    177 rdf:type schema:DefinedTerm
    178 Nbe5df0f3c9604b1dbe849270ec72e94d rdf:first sg:person.01313520764.52
    179 rdf:rest Ne644bc883fe4491194f7bf971b88a673
    180 Nbf0f30f4fc224807879634fc11771a16 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    181 schema:name Glucosephosphate Dehydrogenase
    182 rdf:type schema:DefinedTerm
    183 Nbf4fbb692b334aa6b912725abb33d61e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    184 schema:name Young Adult
    185 rdf:type schema:DefinedTerm
    186 Nc034ce804e904114bb4ce7e0b75eb264 rdf:first sg:person.01143433256.31
    187 rdf:rest Nc0b9ab797d8b4360a420d05fd9e9d887
    188 Nc0b9ab797d8b4360a420d05fd9e9d887 rdf:first sg:person.013056523777.51
    189 rdf:rest N330ac3d3f309499fa9169c68f68c42be
    190 Nc536fd69796f4fa39d955e232fb01e7e rdf:first sg:person.01063334514.37
    191 rdf:rest N9fae443914324f619453c08f142c00cb
    192 Nc56afd9bf0464687af88eafdae0cc12d rdf:first sg:person.01101047413.39
    193 rdf:rest N8dbc9d604e20497993fe3a2a0ce74f02
    194 Ncefdd965f127415f9b1d22100d9a1bd7 rdf:first sg:person.011004004353.45
    195 rdf:rest Nedcf0666695d456ea54b6efc07be4b99
    196 Nd9b166d22f6849fa9cbcfcf2747051fb rdf:first sg:person.01137322113.00
    197 rdf:rest Ndd0454fb7d5946d1bae4525ed91e6fa7
    198 Ndd0454fb7d5946d1bae4525ed91e6fa7 rdf:first N90a1ae502c9e4819afda4e51f8ffbf9a
    199 rdf:rest Nc536fd69796f4fa39d955e232fb01e7e
    200 Nddae330d703c4af48de69d3f89c6b2e6 schema:volumeNumber 18
    201 rdf:type schema:PublicationVolume
    202 Ne0b10bf33bc44ab5b83f00e6d3c2955e schema:name pubmed_id
    203 schema:value 30665411
    204 rdf:type schema:PropertyValue
    205 Ne4220ced7a81449ab83d39d03a4841be schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    206 schema:name Genotyping Techniques
    207 rdf:type schema:DefinedTerm
    208 Ne644bc883fe4491194f7bf971b88a673 rdf:first sg:person.01240573700.51
    209 rdf:rest N9719f25e57c74b578f9c4833812d9a26
    210 Ne94161fe21df44cd8b67ab1ed56de2c3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    211 schema:name Male
    212 rdf:type schema:DefinedTerm
    213 Neb47176ce64c4e628fb0b76a0a2b68bf schema:name doi
    214 schema:value 10.1186/s12936-019-2648-7
    215 rdf:type schema:PropertyValue
    216 Ned6e8b16d2f14a459053d559c7cf91bd schema:affiliation https://www.grid.ac/institutes/grid.8991.9
    217 schema:familyName Mair
    218 schema:givenName Catherine
    219 rdf:type schema:Person
    220 Nedcf0666695d456ea54b6efc07be4b99 rdf:first sg:person.011347720010.59
    221 rdf:rest N96e3e38e8fed4f03bbb646cfd9b429ca
    222 Nef3bc6b1d8814218b9610101a9e51ee8 schema:affiliation https://www.grid.ac/institutes/grid.8991.9
    223 schema:familyName Gonçalves
    224 schema:givenName Bronner P.
    225 rdf:type schema:Person
    226 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    227 schema:name Biological Sciences
    228 rdf:type schema:DefinedTerm
    229 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    230 schema:name Genetics
    231 rdf:type schema:DefinedTerm
    232 sg:grant.2751489 http://pending.schema.org/fundedItem sg:pub.10.1186/s12936-019-2648-7
    233 rdf:type schema:MonetaryGrant
    234 sg:grant.6583834 http://pending.schema.org/fundedItem sg:pub.10.1186/s12936-019-2648-7
    235 rdf:type schema:MonetaryGrant
    236 sg:grant.7389855 http://pending.schema.org/fundedItem sg:pub.10.1186/s12936-019-2648-7
    237 rdf:type schema:MonetaryGrant
    238 sg:journal.1030597 schema:issn 1475-2875
    239 schema:name Malaria Journal
    240 rdf:type schema:Periodical
    241 sg:person.01010116700.30 schema:affiliation https://www.grid.ac/institutes/grid.418150.9
    242 schema:familyName Tiono
    243 schema:givenName Alfred B.
    244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010116700.30
    245 rdf:type schema:Person
    246 sg:person.010201615642.23 schema:affiliation https://www.grid.ac/institutes/grid.8991.9
    247 schema:familyName Clark
    248 schema:givenName Taane G.
    249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010201615642.23
    250 rdf:type schema:Person
    251 sg:person.01023575701.67 schema:affiliation https://www.grid.ac/institutes/grid.10417.33
    252 schema:familyName Bousema
    253 schema:givenName Teun
    254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023575701.67
    255 rdf:type schema:Person
    256 sg:person.01063334514.37 schema:affiliation https://www.grid.ac/institutes/grid.8991.9
    257 schema:familyName d’Alessandro
    258 schema:givenName Umberto
    259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063334514.37
    260 rdf:type schema:Person
    261 sg:person.011004004353.45 schema:affiliation N7a6df9b426ef42eb8efcfa8c4b05c8f1
    262 schema:familyName Curry
    263 schema:givenName Jonathan
    264 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011004004353.45
    265 rdf:type schema:Person
    266 sg:person.01101047413.39 schema:affiliation https://www.grid.ac/institutes/grid.418150.9
    267 schema:familyName Sanou
    268 schema:givenName Guillaume S.
    269 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101047413.39
    270 rdf:type schema:Person
    271 sg:person.011347720010.59 schema:affiliation N43818bb2449243a3878391506fb252ca
    272 schema:familyName Mahey
    273 schema:givenName Laleta
    274 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011347720010.59
    275 rdf:type schema:Person
    276 sg:person.01137322113.00 schema:affiliation https://www.grid.ac/institutes/grid.10417.33
    277 schema:familyName Lanke
    278 schema:givenName Kjerstin H. W.
    279 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137322113.00
    280 rdf:type schema:Person
    281 sg:person.01143433256.31 schema:affiliation N090f20b8d48b417283974d04bd8dcb21
    282 schema:familyName Affara
    283 schema:givenName Muna
    284 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143433256.31
    285 rdf:type schema:Person
    286 sg:person.01163442506.23 schema:affiliation https://www.grid.ac/institutes/grid.8991.9
    287 schema:familyName Grignard
    288 schema:givenName Lynn
    289 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163442506.23
    290 rdf:type schema:Person
    291 sg:person.01240573700.51 schema:affiliation https://www.grid.ac/institutes/grid.418150.9
    292 schema:familyName Coulibaly
    293 schema:givenName Sam A.
    294 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240573700.51
    295 rdf:type schema:Person
    296 sg:person.013056523777.51 schema:affiliation https://www.grid.ac/institutes/grid.418150.9
    297 schema:familyName Ouédraogo
    298 schema:givenName Alphonse
    299 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013056523777.51
    300 rdf:type schema:Person
    301 sg:person.01313520764.52 schema:affiliation N3afd2998fea641eda237c8480aa7fe20
    302 schema:familyName Okebe
    303 schema:givenName Joseph
    304 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313520764.52
    305 rdf:type schema:Person
    306 sg:person.01320377661.56 schema:affiliation https://www.grid.ac/institutes/grid.8991.9
    307 schema:familyName Campino
    308 schema:givenName Susana
    309 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320377661.56
    310 rdf:type schema:Person
    311 sg:person.01323101204.55 schema:affiliation https://www.grid.ac/institutes/grid.418150.9
    312 schema:familyName Bougouma
    313 schema:givenName Edith C.
    314 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01323101204.55
    315 rdf:type schema:Person
    316 sg:person.016327373057.48 schema:affiliation https://www.grid.ac/institutes/grid.8991.9
    317 schema:familyName Drakeley
    318 schema:givenName Chris
    319 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016327373057.48
    320 rdf:type schema:Person
    321 sg:person.0635025104.24 schema:affiliation https://www.grid.ac/institutes/grid.418150.9
    322 schema:familyName Nébié
    323 schema:givenName Issa
    324 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0635025104.24
    325 rdf:type schema:Person
    326 sg:pub.10.1038/321744a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006612157
    327 https://doi.org/10.1038/321744a0
    328 rdf:type schema:CreativeWork
    329 sg:pub.10.1038/35104556 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008333288
    330 https://doi.org/10.1038/35104556
    331 rdf:type schema:CreativeWork
    332 sg:pub.10.1038/ejhg.2009.8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025605016
    333 https://doi.org/10.1038/ejhg.2009.8
    334 rdf:type schema:CreativeWork
    335 sg:pub.10.1038/ncomms1104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015059965
    336 https://doi.org/10.1038/ncomms1104
    337 rdf:type schema:CreativeWork
    338 sg:pub.10.1038/ng.554 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023842929
    339 https://doi.org/10.1038/ng.554
    340 rdf:type schema:CreativeWork
    341 sg:pub.10.1038/s41598-017-10045-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091331754
    342 https://doi.org/10.1038/s41598-017-10045-2
    343 rdf:type schema:CreativeWork
    344 sg:pub.10.1038/s41598-017-14627-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1092371234
    345 https://doi.org/10.1038/s41598-017-14627-y
    346 rdf:type schema:CreativeWork
    347 sg:pub.10.1038/srep00299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031129866
    348 https://doi.org/10.1038/srep00299
    349 rdf:type schema:CreativeWork
    350 sg:pub.10.1038/srep01671 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032689339
    351 https://doi.org/10.1038/srep01671
    352 rdf:type schema:CreativeWork
    353 sg:pub.10.1186/1475-2875-12-286 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045318010
    354 https://doi.org/10.1186/1475-2875-12-286
    355 rdf:type schema:CreativeWork
    356 sg:pub.10.1186/1475-2875-12-418 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004162306
    357 https://doi.org/10.1186/1475-2875-12-418
    358 rdf:type schema:CreativeWork
    359 sg:pub.10.1186/1475-2875-13-148 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044080201
    360 https://doi.org/10.1186/1475-2875-13-148
    361 rdf:type schema:CreativeWork
    362 sg:pub.10.1186/s12916-017-0990-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100473369
    363 https://doi.org/10.1186/s12916-017-0990-6
    364 rdf:type schema:CreativeWork
    365 sg:pub.10.1186/s12936-015-0823-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1010212998
    366 https://doi.org/10.1186/s12936-015-0823-z
    367 rdf:type schema:CreativeWork
    368 sg:pub.10.1186/s12936-015-1077-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020238224
    369 https://doi.org/10.1186/s12936-015-1077-5
    370 rdf:type schema:CreativeWork
    371 sg:pub.10.1186/s12936-017-1889-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1086039400
    372 https://doi.org/10.1186/s12936-017-1889-6
    373 rdf:type schema:CreativeWork
    374 sg:pub.10.1186/s12936-018-2190-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1100555169
    375 https://doi.org/10.1186/s12936-018-2190-z
    376 rdf:type schema:CreativeWork
    377 sg:pub.10.1186/s12936-018-2402-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105341291
    378 https://doi.org/10.1186/s12936-018-2402-6
    379 rdf:type schema:CreativeWork
    380 https://app.dimensions.ai/details/publication/pub.1076027614 schema:CreativeWork
    381 https://app.dimensions.ai/details/publication/pub.1080987014 schema:CreativeWork
    382 https://app.dimensions.ai/details/publication/pub.1082484015 schema:CreativeWork
    383 https://doi.org/10.1016/b978-0-12-407826-0.00004-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042166376
    384 rdf:type schema:CreativeWork
    385 https://doi.org/10.1016/j.bcmd.2012.01.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025191794
    386 rdf:type schema:CreativeWork
    387 https://doi.org/10.1016/j.mbs.2004.01.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013281796
    388 rdf:type schema:CreativeWork
    389 https://doi.org/10.1016/s1473-3099(13)70268-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018876102
    390 rdf:type schema:CreativeWork
    391 https://doi.org/10.1016/s1473-3099(15)00479-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023953646
    392 rdf:type schema:CreativeWork
    393 https://doi.org/10.1086/338628 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058637905
    394 rdf:type schema:CreativeWork
    395 https://doi.org/10.1093/infdis/jiv098 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007915842
    396 rdf:type schema:CreativeWork
    397 https://doi.org/10.1111/j.1365-2141.1977.tb00589.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1018570270
    398 rdf:type schema:CreativeWork
    399 https://doi.org/10.1128/jcm.00904-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050462485
    400 rdf:type schema:CreativeWork
    401 https://doi.org/10.1128/jcm.01683-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020946835
    402 rdf:type schema:CreativeWork
    403 https://doi.org/10.1369/0022155415580594 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050698334
    404 rdf:type schema:CreativeWork
    405 https://doi.org/10.1371/journal.pgen.1004960 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045872687
    406 rdf:type schema:CreativeWork
    407 https://doi.org/10.1371/journal.pmed.1001339 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023509008
    408 rdf:type schema:CreativeWork
    409 https://doi.org/10.1371/journal.pntd.0006230 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103466211
    410 rdf:type schema:CreativeWork
    411 https://doi.org/10.1371/journal.pntd.0006440 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103488674
    412 rdf:type schema:CreativeWork
    413 https://doi.org/10.1371/journal.pone.0169930 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074215508
    414 rdf:type schema:CreativeWork
    415 https://doi.org/10.1371/journal.pone.0190272 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100338217
    416 rdf:type schema:CreativeWork
    417 https://doi.org/10.2144/01303dd04 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074784217
    418 rdf:type schema:CreativeWork
    419 https://doi.org/10.4269/ajtmh.16-0779 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090917394
    420 rdf:type schema:CreativeWork
    421 https://doi.org/10.4269/ajtmh.1996.54.111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082882080
    422 rdf:type schema:CreativeWork
    423 https://doi.org/10.7554/elife.15085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013257417
    424 rdf:type schema:CreativeWork
    425 https://www.grid.ac/institutes/grid.10417.33 schema:alternateName Radboud University Nijmegen Medical Centre
    426 schema:name Department of Immunology and Infection, London School of Hygiene & Tropical Medicine, London, UK
    427 Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands
    428 rdf:type schema:Organization
    429 https://www.grid.ac/institutes/grid.418150.9 schema:alternateName MalariaGEN
    430 schema:name Department of Biomedical Sciences, Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso
    431 rdf:type schema:Organization
    432 https://www.grid.ac/institutes/grid.8991.9 schema:alternateName London School of Hygiene & Tropical Medicine
    433 schema:name Department of Disease Control, London School of Hygiene and Tropical Medicine, London, UK
    434 Department of Immunology and Infection, London School of Hygiene & Tropical Medicine, London, UK
    435 Department of Infectious Disease Epidemiology, London School of Hygiene and Tropical Medicine, London, UK
    436 Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, London, UK
    437 Disease Control & Elimination Theme, Medical Research Council Unit at London School of Hygiene and Tropical Medicine, Fajara, The Gambia
    438 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...